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The map label for this gene is outF [H]

Identifier: 170023255

GI number: 170023255

Start: 1133683

End: 1134885

Strand: Direct

Name: outF [H]

Synonym: YPK_1006

Alternate gene names: 170023255

Gene position: 1133683-1134885 (Clockwise)

Preceding gene: 170023254

Following gene: 170023256

Centisome position: 24.18

GC content: 34.0

Gene sequence:

>1203_bases
ATGGCTGTATTTAAATATGTTGCCATCAGCAGAAGTGGAACCAAGATAACAGGTGATATTGATGCGGAAAACATACGTAT
AGCCCGTTATCTCCTTTACAAAAAAAACATGCATGTCTTAAGTATAAAAAAAAGAATTTTACTTTTTAATAAGTATGTGG
TCAAAAAAAATAGTAATAAGACGGATTTGGTATTAATTACACGGCAGATAGCGACATTAGTAAATGCTTCAATGCCACTA
GATGAGGTTTTAGATATTGTTGGGAAACAAAATAGTAAAAGTAAAATGATTGAAATAATTCAAAGGATTAGAGTTAATAT
ACAGGAAGGGCATTCATTCGCTGATGCGCTATCTCCGTTTTCAGCAGTATTTAGCCCATTATACAAAACGATGGTTACCG
CTGGAGAGGTATCAGGGCATCTGGGATTAGTGCTGGTTCGTCTTGCTGATCATATTGAGCAGACACAAAAAATACAAAGA
AAAATCATTCAGGCACTTATCTACCCTTGTGTGTTGGTTTTAATATCACTAAGTGTCATTATCATATTATTGACAGCGGT
TGTACCGAACATAGTAGAGCAGTTTTCTTTTTCAGAGACGGCGTTACCATTATCAACAAAAGTATTGATGATACTCAGTT
ATAGTATAAAAGAAAATGTTATTTTTATCATGGCTATAGGTGTCTCGGCAGTGATTTTTTTAAATAGATTACTAAAAATA
AATAAGATAAATGTCTTTTTTCATAGGCACTATTTAAGTTTGCCAATGCTGGGTAATATGTTTGTTCGTATAAATACATC
TCGTTATCTTAGAACATTAACAACACTACATTCTAATGGTGTGACAATCGTACAGGCAATGAGTATTAGTAATGCGGTAT
TAACGAATGTATATATAAAAAATAAGTTAAACATTTCAGTTAAATTGGTCAGTGAGGGGTGTAGTTTATCGTCATCTTTA
GTTGATAGTGGAGTATTCCCCCCGATCATATTACATATGATCATATCGGGGGAGCGTAGTGGTGAGTTGGACCATATGCT
AGAAACTGTGGCGGGTGTCCAGGAAGAAGAACTGATGAATCAAATAAGTATAGTAATGTCATTGTTAGAGCCAACCATAA
TTATAGTTATGGCCGCGTTTATTTCATTTGTTATTCTGTCAATTCTTCAGCCAATACTTGAAATTAATAGCCTTGTCATG
TAA

Upstream 100 bases:

>100_bases
AGTGGGGCTATAAACAGCCTTCTATCTGATGGGATAAGCAAAGTTATTGCAGGGTTAACCACTATAGAGGAACTGGTAAG
AATATGCCAAGAGGATGATA

Downstream 100 bases:

>100_bases
TTAATTTTATGAGGGAATAATGGCAAACAAAAAAACAAAAGGATTTACATTGTTGGAAATTATGGTGGTTATTGTTATTC
TGGGCCTACTTGCTAGCTTA

Product: type II secretion system protein

Products: NA

Alternate protein names: Pectic enzymes secretion protein outF [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MAVFKYVAISRSGTKITGDIDAENIRIARYLLYKKNMHVLSIKKRILLFNKYVVKKNSNKTDLVLITRQIATLVNASMPL
DEVLDIVGKQNSKSKMIEIIQRIRVNIQEGHSFADALSPFSAVFSPLYKTMVTAGEVSGHLGLVLVRLADHIEQTQKIQR
KIIQALIYPCVLVLISLSVIIILLTAVVPNIVEQFSFSETALPLSTKVLMILSYSIKENVIFIMAIGVSAVIFLNRLLKI
NKINVFFHRHYLSLPMLGNMFVRINTSRYLRTLTTLHSNGVTIVQAMSISNAVLTNVYIKNKLNISVKLVSEGCSLSSSL
VDSGVFPPIILHMIISGERSGELDHMLETVAGVQEEELMNQISIVMSLLEPTIIIVMAAFISFVILSILQPILEINSLVM

Sequences:

>Translated_400_residues
MAVFKYVAISRSGTKITGDIDAENIRIARYLLYKKNMHVLSIKKRILLFNKYVVKKNSNKTDLVLITRQIATLVNASMPL
DEVLDIVGKQNSKSKMIEIIQRIRVNIQEGHSFADALSPFSAVFSPLYKTMVTAGEVSGHLGLVLVRLADHIEQTQKIQR
KIIQALIYPCVLVLISLSVIIILLTAVVPNIVEQFSFSETALPLSTKVLMILSYSIKENVIFIMAIGVSAVIFLNRLLKI
NKINVFFHRHYLSLPMLGNMFVRINTSRYLRTLTTLHSNGVTIVQAMSISNAVLTNVYIKNKLNISVKLVSEGCSLSSSL
VDSGVFPPIILHMIISGERSGELDHMLETVAGVQEEELMNQISIVMSLLEPTIIIVMAAFISFVILSILQPILEINSLVM
>Mature_399_residues
AVFKYVAISRSGTKITGDIDAENIRIARYLLYKKNMHVLSIKKRILLFNKYVVKKNSNKTDLVLITRQIATLVNASMPLD
EVLDIVGKQNSKSKMIEIIQRIRVNIQEGHSFADALSPFSAVFSPLYKTMVTAGEVSGHLGLVLVRLADHIEQTQKIQRK
IIQALIYPCVLVLISLSVIIILLTAVVPNIVEQFSFSETALPLSTKVLMILSYSIKENVIFIMAIGVSAVIFLNRLLKIN
KINVFFHRHYLSLPMLGNMFVRINTSRYLRTLTTLHSNGVTIVQAMSISNAVLTNVYIKNKLNISVKLVSEGCSLSSSLV
DSGVFPPIILHMIISGERSGELDHMLETVAGVQEEELMNQISIVMSLLEPTIIIVMAAFISFVILSILQPILEINSLVM

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of the multiple pectic enzymes [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=405, Percent_Identity=38.5185185185185, Blast_Score=292, Evalue=2e-80,
Organism=Escherichia coli, GI1786295, Length=395, Percent_Identity=23.5443037974684, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 44565; Mature: 44434

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS00874 T2SP_F

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVFKYVAISRSGTKITGDIDAENIRIARYLLYKKNMHVLSIKKRILLFNKYVVKKNSNK
CCEEEEEEEECCCCEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEECCCCC
TDLVLITRQIATLVNASMPLDEVLDIVGKQNSKSKMIEIIQRIRVNIQEGHSFADALSPF
CEEEEHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
SAVFSPLYKTMVTAGEVSGHLGLVLVRLADHIEQTQKIQRKIIQALIYPCVLVLISLSVI
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IILLTAVVPNIVEQFSFSETALPLSTKVLMILSYSIKENVIFIMAIGVSAVIFLNRLLKI
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHH
NKINVFFHRHYLSLPMLGNMFVRINTSRYLRTLTTLHSNGVTIVQAMSISNAVLTNVYIK
HHEEEEEEHHHHCCCCCCCEEEEEEHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHEEEE
NKLNISVKLVSEGCSLSSSLVDSGVFPPIILHMIISGERSGELDHMLETVAGVQEEELMN
CCCCEEEEEECCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHH
QISIVMSLLEPTIIIVMAAFISFVILSILQPILEINSLVM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AVFKYVAISRSGTKITGDIDAENIRIARYLLYKKNMHVLSIKKRILLFNKYVVKKNSNK
CEEEEEEEECCCCEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEECCCCC
TDLVLITRQIATLVNASMPLDEVLDIVGKQNSKSKMIEIIQRIRVNIQEGHSFADALSPF
CEEEEHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
SAVFSPLYKTMVTAGEVSGHLGLVLVRLADHIEQTQKIQRKIIQALIYPCVLVLISLSVI
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IILLTAVVPNIVEQFSFSETALPLSTKVLMILSYSIKENVIFIMAIGVSAVIFLNRLLKI
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHH
NKINVFFHRHYLSLPMLGNMFVRINTSRYLRTLTTLHSNGVTIVQAMSISNAVLTNVYIK
HHEEEEEEHHHHCCCCCCCEEEEEEHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHEEEE
NKLNISVKLVSEGCSLSSSLVDSGVFPPIILHMIISGERSGELDHMLETVAGVQEEELMN
CCCCEEEEEECCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHH
QISIVMSLLEPTIIIVMAAFISFVILSILQPILEINSLVM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8326859 [H]