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The map label for this gene is cycB [H]

Identifier: 170023217

GI number: 170023217

Start: 1085839

End: 1087074

Strand: Direct

Name: cycB [H]

Synonym: YPK_0968

Alternate gene names: 170023217

Gene position: 1085839-1087074 (Clockwise)

Preceding gene: 170023208

Following gene: 170023218

Centisome position: 23.15

GC content: 47.57

Gene sequence:

>1236_bases
ATGAAAATCAATAAAATCACTGCGCTTGTTTTAACCGCACTGGCAGTCACCCAGTTTGCTGGTTTCGCTGCCCACGCTGC
TACACAGCAATTAACAGTTTGGGAAGACATCAAAAAATCTGCCTGTATTAAAGAGGCTATCGCTGATTTTGAAAAACAGC
ATCAGGTTAAGGTCAATGTGCTGGAAATGCCTTACGCACAACAAATTGAAAAACTCCGCCTTGATGGCCCTGCAGGTATC
GGCCCTGATGTGTTGGTGATTCCCAATGATCAGTTAGGTGGTGCGGTAGTGCAAGGTTTGCTGACACCGCTCAGCGTTGA
TCCAACCATAGTCACTACTTTTACTAAACCTTCTATCGCTGCCTTCACCATGGATAATGCCCTCTACGGTTTACCGAAAG
CCGTGGAAACGCTGGTGATGATCTACAACAAAGACATGCTGCCAACGCCGTTAGCTACCTTGGATGAGTACGCCGCATTC
TCTAAGAAACAACGCGCAGAAAATAAATATGGTCTGTTGGCGAAGTTCGATCAGATCTATTACAGCTGGGGAGCGATTGA
GCCAATGGGCGGTTACATCTTTGGTAAAGATGCTAACGGTAGCTTGAAGGCTAACGATATCGGGCTAAATACGCCAGGGG
CTGTTGAGGCCGTAACCTATTTGAAAACATTCTATGCTAACGGTCTGTTTCCAATTGGCACCATCGGTGATAACGGCTTG
AATGCTATTGACTCATTATTCACTGAGAAAAAAGCGGCTGCGGTAATTAACGGGCCATGGGCATTCCAACCGTATGAAGC
CGCTGGTATTAACTTTGGTGTGTCACCACTGCCAGCATTACCGAACGGCAAAGATATGAGCTCCTTCCTCGGTGTGAAAG
GGTATGTCGTTTCTACCTGGAGCAAAGATAAGGCACTCGCCCAGCAGTTCATCGAATTTATTAACCAACCGCAATACGTG
AAAACCCGCTATCAGGTCACCAAAGAGATCCCCGCGTTGACGGCCATGATTGACGATCCATTGATTAAAAATGATGAAAA
AGCCAGTGCGGTAGCCATTCAGGCAAGCCGTGCCTCTGCGATGCCTGGTATTCCAGAAATGGGCGAAGTGTGGGGACCTG
CGAACTCAGCATTGGAGCTAAGCGTAACGGGCAAACAGGAGCCTAAAGTCGCTCTCGATAACGCCGTTAAGCAGATCAAT
ATGCAAATCGAGGCCATGCAGGCCAGTAATCAGTAA

Upstream 100 bases:

>100_bases
TGTTCTGCAGATGGCGTAGATGTGGTGTTTGTCATTTTGTATTTGTATTTAATCCCGAGCGTTAAACACAATAATGTTAT
ACCTTCACCCGAGGCTAATA

Downstream 100 bases:

>100_bases
TCAGACTATTTAACAGGTCAGTATGAATTTAGGTGGTGTTCAATTCCCCCACGTGCCGTTGGTATGCGATGGTTTTTGTG
CACCACCTGTGTTTCAAACT

Product: extracellular solute-binding protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MKINKITALVLTALAVTQFAGFAAHAATQQLTVWEDIKKSACIKEAIADFEKQHQVKVNVLEMPYAQQIEKLRLDGPAGI
GPDVLVIPNDQLGGAVVQGLLTPLSVDPTIVTTFTKPSIAAFTMDNALYGLPKAVETLVMIYNKDMLPTPLATLDEYAAF
SKKQRAENKYGLLAKFDQIYYSWGAIEPMGGYIFGKDANGSLKANDIGLNTPGAVEAVTYLKTFYANGLFPIGTIGDNGL
NAIDSLFTEKKAAAVINGPWAFQPYEAAGINFGVSPLPALPNGKDMSSFLGVKGYVVSTWSKDKALAQQFIEFINQPQYV
KTRYQVTKEIPALTAMIDDPLIKNDEKASAVAIQASRASAMPGIPEMGEVWGPANSALELSVTGKQEPKVALDNAVKQIN
MQIEAMQASNQ

Sequences:

>Translated_411_residues
MKINKITALVLTALAVTQFAGFAAHAATQQLTVWEDIKKSACIKEAIADFEKQHQVKVNVLEMPYAQQIEKLRLDGPAGI
GPDVLVIPNDQLGGAVVQGLLTPLSVDPTIVTTFTKPSIAAFTMDNALYGLPKAVETLVMIYNKDMLPTPLATLDEYAAF
SKKQRAENKYGLLAKFDQIYYSWGAIEPMGGYIFGKDANGSLKANDIGLNTPGAVEAVTYLKTFYANGLFPIGTIGDNGL
NAIDSLFTEKKAAAVINGPWAFQPYEAAGINFGVSPLPALPNGKDMSSFLGVKGYVVSTWSKDKALAQQFIEFINQPQYV
KTRYQVTKEIPALTAMIDDPLIKNDEKASAVAIQASRASAMPGIPEMGEVWGPANSALELSVTGKQEPKVALDNAVKQIN
MQIEAMQASNQ
>Mature_411_residues
MKINKITALVLTALAVTQFAGFAAHAATQQLTVWEDIKKSACIKEAIADFEKQHQVKVNVLEMPYAQQIEKLRLDGPAGI
GPDVLVIPNDQLGGAVVQGLLTPLSVDPTIVTTFTKPSIAAFTMDNALYGLPKAVETLVMIYNKDMLPTPLATLDEYAAF
SKKQRAENKYGLLAKFDQIYYSWGAIEPMGGYIFGKDANGSLKANDIGLNTPGAVEAVTYLKTFYANGLFPIGTIGDNGL
NAIDSLFTEKKAAAVINGPWAFQPYEAAGINFGVSPLPALPNGKDMSSFLGVKGYVVSTWSKDKALAQQFIEFINQPQYV
KTRYQVTKEIPALTAMIDDPLIKNDEKASAVAIQASRASAMPGIPEMGEVWGPANSALELSVTGKQEPKVALDNAVKQIN
MQIEAMQASNQ

Specific function: Binding protein for cyclodextrin; involved in its cellular uptake. Interacts with all natural cyclodextrins:alpha, beta and gamma [H]

COG id: COG2182

COG function: function code G; Maltose-binding periplasmic proteins/domains

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790466, Length=398, Percent_Identity=29.3969849246231, Blast_Score=144, Evalue=1e-35,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006060
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 44284; Mature: 44284

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKINKITALVLTALAVTQFAGFAAHAATQQLTVWEDIKKSACIKEAIADFEKQHQVKVNV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
LEMPYAQQIEKLRLDGPAGIGPDVLVIPNDQLGGAVVQGLLTPLSVDPTIVTTFTKPSIA
ECCCHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHCCCCCCCEEEEEECCCCEE
AFTMDNALYGLPKAVETLVMIYNKDMLPTPLATLDEYAAFSKKQRAENKYGLLAKFDQIY
EEEECCHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
YSWGAIEPMGGYIFGKDANGSLKANDIGLNTPGAVEAVTYLKTFYANGLFPIGTIGDNGL
HHCCCCCCCCCEEEECCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCHH
NAIDSLFTEKKAAAVINGPWAFQPYEAAGINFGVSPLPALPNGKDMSSFLGVKGYVVSTW
HHHHHHHHHHHCCEEECCCCCCCCCHHCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEECC
SKDKALAQQFIEFINQPQYVKTRYQVTKEIPALTAMIDDPLIKNDEKASAVAIQASRASA
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHCCC
MPGIPEMGEVWGPANSALELSVTGKQEPKVALDNAVKQINMQIEAMQASNQ
CCCCCCHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHEEEECCCCC
>Mature Secondary Structure
MKINKITALVLTALAVTQFAGFAAHAATQQLTVWEDIKKSACIKEAIADFEKQHQVKVNV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
LEMPYAQQIEKLRLDGPAGIGPDVLVIPNDQLGGAVVQGLLTPLSVDPTIVTTFTKPSIA
ECCCHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHCCCCCCCEEEEEECCCCEE
AFTMDNALYGLPKAVETLVMIYNKDMLPTPLATLDEYAAFSKKQRAENKYGLLAKFDQIY
EEEECCHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
YSWGAIEPMGGYIFGKDANGSLKANDIGLNTPGAVEAVTYLKTFYANGLFPIGTIGDNGL
HHCCCCCCCCCEEEECCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCHH
NAIDSLFTEKKAAAVINGPWAFQPYEAAGINFGVSPLPALPNGKDMSSFLGVKGYVVSTW
HHHHHHHHHHHCCEEECCCCCCCCCHHCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEECC
SKDKALAQQFIEFINQPQYVKTRYQVTKEIPALTAMIDDPLIKNDEKASAVAIQASRASA
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHCCC
MPGIPEMGEVWGPANSALELSVTGKQEPKVALDNAVKQINMQIEAMQASNQ
CCCCCCHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]