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The map label for this gene is intS [H]

Identifier: 170023174

GI number: 170023174

Start: 1044904

End: 1046118

Strand: Direct

Name: intS [H]

Synonym: YPK_0925

Alternate gene names: 170023174

Gene position: 1044904-1046118 (Clockwise)

Preceding gene: 170023173

Following gene: 170023192

Centisome position: 22.28

GC content: 48.72

Gene sequence:

>1215_bases
ATGAAGCTAAACGCCCGGCAGGTTGAAACAGCCAAACCCAAAGACAAAGCCTATAAGCTCGCGGATGGGGGCGGTTTGTA
CTTATTGGTCAATACCAATGGCTCGCGTTATTGGCGTTTAAAGTACCGCTTTGCGGGGAAAGAGAAGTTGTTGGCCGTGG
GGGTGTATCCAGACGTGTCGTTAGCGGTTGCCAGAGTAAAACGTGACGAGGCTAAGAAGATTGTCGCTGGTGGCGGTGAT
CCCAGCCAGAACAAACAACAGGAAAAACTAGCCCGACAAGGTGAAGCGACGAATACCTTTGAAGCTATCACCCGAGAGTG
GTATCAACGGCGTTACGATAGGTGGTCTGAATCTTATCGTTTAGAAATGATGAGCACGTTTGAAAGTGACGTTTTTCCAT
ATATCGGTTACCGGCCAATCAAAGAAATTAAACCGCTTGAGCTGATGGCCGTGCTGTCCAAGTTAGAAAAACGGGGTGCC
ACTGAGAAAATGCGTAAGGTTCGGCAGCGATGTGGTGAGGTTTGGAAATACGCCATCATCACAGGCCGAGCCGAATATAA
TCCCGCCCCTGACCTTGCCAGTGCTTTTGCTCCTCATAAACGCGAACACTATCCACATCTTACGATTAATGAAATCCCTG
AATTTCTTTCCAGCCTTGCCAGTTATAGCGGCAGCATGTTGGTTAAGTTGGCTATGCGGTTGTTGATCCTCACCGGTACC
CGCCCAGGTGAATTGCGGCAGGCCGAATGGGCAGAGTTTGATTTTGATAATGCCTTATGGGAGATCCCCGCGGTGCGAAT
GAAGATGCGTCGGCCACATATGGTGCCGCTTCCGGATCAAGCCATTACGATACTAAAGCAGATTCAACCTATCAGCGGGC
GCTATCAGTTTGTGTTCCCTGGCAGAATACAGCACAGCAAGCCTATCAGTGAGATGACACTCAATGTACTAATCCGACGT
ATCGGTTATGGCGGCAGAGCCACTGGACATGGTTTTCGCCACACAATGAGCACTATTTTGCATGAGCAGGGTTTCAATAC
GGCCTGGATAGAAACACAACTGGCTCATGTCGATAAAAACAGTATTCGCGGTACTTATAATCACGCTCAATATCTGGATG
GTCGCAGAGAAATGCTGCAATGGTATGCCGACTATATGGATAGTCTGGAAAATGGCGAGAATGTGATTCATGGCAGATTT
GGGAAACGGGCATAG

Upstream 100 bases:

>100_bases
TCTAGTTATTCCTATATACATGTTCGTCTATTGAAATCTATTTATAGCGGGGGGTATATATGGGGGTATCAAATTATACC
CACTTAAAGGGTACCCCCAG

Downstream 100 bases:

>100_bases
GGGAGGGATATTTGCTATCTGGCTGAAATAGTTATCCAAACTTTTTAAAGGCATCTCACGACGCGTTTATATTTAAACTA
ACAACTTGAAATTAAAGGAT

Product: integrase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MKLNARQVETAKPKDKAYKLADGGGLYLLVNTNGSRYWRLKYRFAGKEKLLAVGVYPDVSLAVARVKRDEAKKIVAGGGD
PSQNKQQEKLARQGEATNTFEAITREWYQRRYDRWSESYRLEMMSTFESDVFPYIGYRPIKEIKPLELMAVLSKLEKRGA
TEKMRKVRQRCGEVWKYAIITGRAEYNPAPDLASAFAPHKREHYPHLTINEIPEFLSSLASYSGSMLVKLAMRLLILTGT
RPGELRQAEWAEFDFDNALWEIPAVRMKMRRPHMVPLPDQAITILKQIQPISGRYQFVFPGRIQHSKPISEMTLNVLIRR
IGYGGRATGHGFRHTMSTILHEQGFNTAWIETQLAHVDKNSIRGTYNHAQYLDGRREMLQWYADYMDSLENGENVIHGRF
GKRA

Sequences:

>Translated_404_residues
MKLNARQVETAKPKDKAYKLADGGGLYLLVNTNGSRYWRLKYRFAGKEKLLAVGVYPDVSLAVARVKRDEAKKIVAGGGD
PSQNKQQEKLARQGEATNTFEAITREWYQRRYDRWSESYRLEMMSTFESDVFPYIGYRPIKEIKPLELMAVLSKLEKRGA
TEKMRKVRQRCGEVWKYAIITGRAEYNPAPDLASAFAPHKREHYPHLTINEIPEFLSSLASYSGSMLVKLAMRLLILTGT
RPGELRQAEWAEFDFDNALWEIPAVRMKMRRPHMVPLPDQAITILKQIQPISGRYQFVFPGRIQHSKPISEMTLNVLIRR
IGYGGRATGHGFRHTMSTILHEQGFNTAWIETQLAHVDKNSIRGTYNHAQYLDGRREMLQWYADYMDSLENGENVIHGRF
GKRA
>Mature_404_residues
MKLNARQVETAKPKDKAYKLADGGGLYLLVNTNGSRYWRLKYRFAGKEKLLAVGVYPDVSLAVARVKRDEAKKIVAGGGD
PSQNKQQEKLARQGEATNTFEAITREWYQRRYDRWSESYRLEMMSTFESDVFPYIGYRPIKEIKPLELMAVLSKLEKRGA
TEKMRKVRQRCGEVWKYAIITGRAEYNPAPDLASAFAPHKREHYPHLTINEIPEFLSSLASYSGSMLVKLAMRLLILTGT
RPGELRQAEWAEFDFDNALWEIPAVRMKMRRPHMVPLPDQAITILKQIQPISGRYQFVFPGRIQHSKPISEMTLNVLIRR
IGYGGRATGHGFRHTMSTILHEQGFNTAWIETQLAHVDKNSIRGTYNHAQYLDGRREMLQWYADYMDSLENGENVIHGRF
GKRA

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788690, Length=386, Percent_Identity=44.559585492228, Blast_Score=354, Evalue=4e-99,
Organism=Escherichia coli, GI145693166, Length=393, Percent_Identity=42.4936386768448, Blast_Score=300, Evalue=9e-83,
Organism=Escherichia coli, GI1788974, Length=396, Percent_Identity=37.6262626262626, Blast_Score=292, Evalue=3e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 46546; Mature: 46546

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNARQVETAKPKDKAYKLADGGGLYLLVNTNGSRYWRLKYRFAGKEKLLAVGVYPDVS
CCCCCCCCCCCCCHHHCEEEECCCCEEEEEECCCCEEEEEEEEECCCCEEEEEECCCCHH
LAVARVKRDEAKKIVAGGGDPSQNKQQEKLARQGEATNTFEAITREWYQRRYDRWSESYR
HHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LEMMSTFESDVFPYIGYRPIKEIKPLELMAVLSKLEKRGATEKMRKVRQRCGEVWKYAII
HHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEHEE
TGRAEYNPAPDLASAFAPHKREHYPHLTINEIPEFLSSLASYSGSMLVKLAMRLLILTGT
ECCCCCCCCCCHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
RPGELRQAEWAEFDFDNALWEIPAVRMKMRRPHMVPLPDQAITILKQIQPISGRYQFVFP
CCCCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEC
GRIQHSKPISEMTLNVLIRRIGYGGRATGHGFRHTMSTILHEQGFNTAWIETQLAHVDKN
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCHH
SIRGTYNHAQYLDGRREMLQWYADYMDSLENGENVIHGRFGKRA
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCC
>Mature Secondary Structure
MKLNARQVETAKPKDKAYKLADGGGLYLLVNTNGSRYWRLKYRFAGKEKLLAVGVYPDVS
CCCCCCCCCCCCCHHHCEEEECCCCEEEEEECCCCEEEEEEEEECCCCEEEEEECCCCHH
LAVARVKRDEAKKIVAGGGDPSQNKQQEKLARQGEATNTFEAITREWYQRRYDRWSESYR
HHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LEMMSTFESDVFPYIGYRPIKEIKPLELMAVLSKLEKRGATEKMRKVRQRCGEVWKYAII
HHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEHEE
TGRAEYNPAPDLASAFAPHKREHYPHLTINEIPEFLSSLASYSGSMLVKLAMRLLILTGT
ECCCCCCCCCCHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
RPGELRQAEWAEFDFDNALWEIPAVRMKMRRPHMVPLPDQAITILKQIQPISGRYQFVFP
CCCCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEC
GRIQHSKPISEMTLNVLIRRIGYGGRATGHGFRHTMSTILHEQGFNTAWIETQLAHVDKN
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCHH
SIRGTYNHAQYLDGRREMLQWYADYMDSLENGENVIHGRFGKRA
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]