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The map label for this gene is gshB [H]

Identifier: 170023087

GI number: 170023087

Start: 960231

End: 961190

Strand: Reverse

Name: gshB [H]

Synonym: YPK_0838

Alternate gene names: 170023087

Gene position: 961190-960231 (Counterclockwise)

Preceding gene: 170023088

Following gene: 170023086

Centisome position: 20.5

GC content: 49.17

Gene sequence:

>960_bases
ATGATTAAGCTCGGTATCGTAATGGACCCTATTTCGTCCATTAACATCAAGAAAGACACCAGTTTCGCTATGCTATTAGA
AGCGCAGCGCCGTGGCTGGGAATTGCATTACATGGAGATGGGGGATCTCTATATGCGTGGGGGTGATGGCCGCGCACGTA
CCCGCCGACTGAATGTGAAGCAGGATAAAGATAACTGGTTCAGTTTTGGGGCTGAGCAGGACCTGCCGCTCTATGATCTT
GATGTCATTCTGATGCGTAAAGATCCGCCGTTTGATACCGAGTTTATCTATGCCACCTATATTCTGGAACGTGCAGAAGA
CAAAGGCACACTGGTGGTGAATAAGCCGCAAAGCCTACGCGATTGTAACGAAAAACTGTTTACGGCTTGGTTCCCAGAGT
TAACCCCAGATACGCTGGTCAGCCGCAGTAAAGACCATATCCGCAAATTCCACCAGGAACACGGCGATATCATCCTGAAA
CCACTGGATGGCATGGGGGGTACCTCAATATTCAGAGTAAAACAAGATGACCCTAACCTCTCGGTTATCATTGAAACACT
GACCGAATTAGGCAGCCGCTTCTGCATGGCACAAAACTTCTTACCGGCCATTAAAGAAGGCGATAAACGTGTGCTAGTGG
TTGATGGCGAGCCTGTGCCTTATTGTCTGGCACGTATTCCCGCCCAAGGTGAAACCCGTGGCAATCTGGCTGCCGGTGGC
CGTGGCGAAGCTCGCCCATTGAGTGAAAGTGATTGGGAAATTGCCCGCACTGTTGCACCAATACTGAAGCAAAAAGGCTT
AATTTTTGTTGGGTTGGATATTATTGGTGATCGCTTGACTGAAATTAACGTCACCAGCCCGACCTGCGTGCGTGAAATCG
AAGCGGCCTTCCCCGAGATTTCCATTACCGGTATGTTAATGGACGCCATTGAAAGACGTTTGGCTGACAAAAAGGCCTAA

Upstream 100 bases:

>100_bases
CGAGTGCTGCGAACAGAAACTACCGCCCTTACCGCAATTACTGCGTTGCAGGTGCGGTTTGGCGATCTGGGATAAACCCC
TTTCCCACTAGGAGAAAAGA

Downstream 100 bases:

>100_bases
TACATAAAACGGGGTGTGGTGTTTTTCTAAGCGCTGCCCCCGTGACTTTATCCTCATTTATCCGCATACTGGCACCCGTT
CATCCATTAACTCAGCGACT

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MIKLGIVMDPISSINIKKDTSFAMLLEAQRRGWELHYMEMGDLYMRGGDGRARTRRLNVKQDKDNWFSFGAEQDLPLYDL
DVILMRKDPPFDTEFIYATYILERAEDKGTLVVNKPQSLRDCNEKLFTAWFPELTPDTLVSRSKDHIRKFHQEHGDIILK
PLDGMGGTSIFRVKQDDPNLSVIIETLTELGSRFCMAQNFLPAIKEGDKRVLVVDGEPVPYCLARIPAQGETRGNLAAGG
RGEARPLSESDWEIARTVAPILKQKGLIFVGLDIIGDRLTEINVTSPTCVREIEAAFPEISITGMLMDAIERRLADKKA

Sequences:

>Translated_319_residues
MIKLGIVMDPISSINIKKDTSFAMLLEAQRRGWELHYMEMGDLYMRGGDGRARTRRLNVKQDKDNWFSFGAEQDLPLYDL
DVILMRKDPPFDTEFIYATYILERAEDKGTLVVNKPQSLRDCNEKLFTAWFPELTPDTLVSRSKDHIRKFHQEHGDIILK
PLDGMGGTSIFRVKQDDPNLSVIIETLTELGSRFCMAQNFLPAIKEGDKRVLVVDGEPVPYCLARIPAQGETRGNLAAGG
RGEARPLSESDWEIARTVAPILKQKGLIFVGLDIIGDRLTEINVTSPTCVREIEAAFPEISITGMLMDAIERRLADKKA
>Mature_319_residues
MIKLGIVMDPISSINIKKDTSFAMLLEAQRRGWELHYMEMGDLYMRGGDGRARTRRLNVKQDKDNWFSFGAEQDLPLYDL
DVILMRKDPPFDTEFIYATYILERAEDKGTLVVNKPQSLRDCNEKLFTAWFPELTPDTLVSRSKDHIRKFHQEHGDIILK
PLDGMGGTSIFRVKQDDPNLSVIIETLTELGSRFCMAQNFLPAIKEGDKRVLVVDGEPVPYCLARIPAQGETRGNLAAGG
RGEARPLSESDWEIARTVAPILKQKGLIFVGLDIIGDRLTEINVTSPTCVREIEAAFPEISITGMLMDAIERRLADKKA

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=317, Percent_Identity=80.4416403785489, Blast_Score=533, Evalue=1e-153,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 35986; Mature: 35986

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLGIVMDPISSINIKKDTSFAMLLEAQRRGWELHYMEMGDLYMRGGDGRARTRRLNVK
CEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCEEEECCCCCCEEEECCCC
QDKDNWFSFGAEQDLPLYDLDVILMRKDPPFDTEFIYATYILERAEDKGTLVVNKPQSLR
CCCCCCEECCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEECCHHHH
DCNEKLFTAWFPELTPDTLVSRSKDHIRKFHQEHGDIILKPLDGMGGTSIFRVKQDDPNL
HHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCCCCH
SVIIETLTELGSRFCMAQNFLPAIKEGDKRVLVVDGEPVPYCLARIPAQGETRGNLAAGG
HHHHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEECCCCCCHHHHCCCCCCCCCCCCCCCC
RGEARPLSESDWEIARTVAPILKQKGLIFVGLDIIGDRLTEINVTSPTCVREIEAAFPEI
CCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEHHHHCCCCEEEECCCCHHHHHHHHHCCCE
SITGMLMDAIERRLADKKA
EEHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIKLGIVMDPISSINIKKDTSFAMLLEAQRRGWELHYMEMGDLYMRGGDGRARTRRLNVK
CEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCEEEECCCCCCEEEECCCC
QDKDNWFSFGAEQDLPLYDLDVILMRKDPPFDTEFIYATYILERAEDKGTLVVNKPQSLR
CCCCCCEECCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEECCHHHH
DCNEKLFTAWFPELTPDTLVSRSKDHIRKFHQEHGDIILKPLDGMGGTSIFRVKQDDPNL
HHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCCCCH
SVIIETLTELGSRFCMAQNFLPAIKEGDKRVLVVDGEPVPYCLARIPAQGETRGNLAAGG
HHHHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEECCCCCCHHHHCCCCCCCCCCCCCCCC
RGEARPLSESDWEIARTVAPILKQKGLIFVGLDIIGDRLTEINVTSPTCVREIEAAFPEI
CCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEHHHHCCCCEEEECCCCHHHHHHHHHCCCE
SITGMLMDAIERRLADKKA
EEHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]