Click here to switch to the map view.

The map label for this gene is 170023084

Identifier: 170023084

GI number: 170023084

Start: 958066

End: 958950

Strand: Reverse

Name: 170023084

Synonym: YPK_0835

Alternate gene names: NA

Gene position: 958950-958066 (Counterclockwise)

Preceding gene: 170023085

Following gene: 170023083

Centisome position: 20.45

GC content: 50.62

Gene sequence:

>885_bases
ATGACAAAAGTGACTGTTGCTGCGACTCAAATGGCCTGTTCTTGGGACCTGCCGAAAAACATTGAAAATGCCGAAAAATT
GGTACGACAGGCACATGCCAGAGGCGCACAGATCATCCTGATTCAGGAGCTGTTTGCCGCACCTTACTTCTGCATTGATC
AAAGCCCAGAGCACTACGCACTGGCCCAAGAGCTGGATAATAGTCCATTAATTAAACACTTCTCCAAGCTTGCCGCTGAG
CTGGAAGTGGTACTACCGCTGAGTTTCTTTGAGAAAGCCAATAATGCTTACTATAACTCGCTAGTCATGATTGACGCCGA
TGGCTCGGTGCTGGACGTCTACCGTAAAACCCACATTCCTAATGGCCCGGCTTATCAGGAGAAACAGTTCTTTATCCCTG
GAGATACCGGTTTTAAAGTTTGGCAAACCCGTTATGCCAAAGTGGGCGTAGGCATCTGTTGGGATCAGTGGTTCCCTGAA
ACCGCGCGCAGCCTGGCACTGCTTGGGGCAGAAGTTATCTTCTACCCAACAGCAATCGGCTCAGAACCTGCTTACCCTGA
AATTGACAGCCAACCGCACTGGACCCGTGTCCAGCAAGGCCATGCAGCCGCAAACCTGGTTCCAGTGATTGCCTCTAACC
GCATCGGTACTGAGAAAAGTAAATACATTGATGGGCTGGAGATGACGTTCTACGGCTCCTCGTTTATTGCTGACCAAACT
GGCGCGCTGTTAGCTCAAGCTAACAAAACTGACGAAGCGGTTCTGGTGCATGAATTTGATTTGCAGGAGATCGCCGCCCA
ACGTGCTTCATGGGGCTTGTTCCGTGATCGCCGCCCAGAGATGTACCAAACATTAGCCACGTCAGACGGCAAGACCCGGA
GATAA

Upstream 100 bases:

>100_bases
TGTATACTTATAACTTTGTATACTGCGGCGATGCCAATCAGCAGTTTAAAATATTGCACACCTCTTGCAGGTGTTTTTTT
ACATTTATTGAGGTTTTCTC

Downstream 100 bases:

>100_bases
TTTATGTCGGTGCAAGACACGATGTTACAACAACAGGCATTACCCGGCACACCGCGGCAGGATGGTTTCTTTATGCCTGC
AGAATGGGCACCGCAGGATG

Product: N-carbamoylputrescine amidase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MTKVTVAATQMACSWDLPKNIENAEKLVRQAHARGAQIILIQELFAAPYFCIDQSPEHYALAQELDNSPLIKHFSKLAAE
LEVVLPLSFFEKANNAYYNSLVMIDADGSVLDVYRKTHIPNGPAYQEKQFFIPGDTGFKVWQTRYAKVGVGICWDQWFPE
TARSLALLGAEVIFYPTAIGSEPAYPEIDSQPHWTRVQQGHAAANLVPVIASNRIGTEKSKYIDGLEMTFYGSSFIADQT
GALLAQANKTDEAVLVHEFDLQEIAAQRASWGLFRDRRPEMYQTLATSDGKTRR

Sequences:

>Translated_294_residues
MTKVTVAATQMACSWDLPKNIENAEKLVRQAHARGAQIILIQELFAAPYFCIDQSPEHYALAQELDNSPLIKHFSKLAAE
LEVVLPLSFFEKANNAYYNSLVMIDADGSVLDVYRKTHIPNGPAYQEKQFFIPGDTGFKVWQTRYAKVGVGICWDQWFPE
TARSLALLGAEVIFYPTAIGSEPAYPEIDSQPHWTRVQQGHAAANLVPVIASNRIGTEKSKYIDGLEMTFYGSSFIADQT
GALLAQANKTDEAVLVHEFDLQEIAAQRASWGLFRDRRPEMYQTLATSDGKTRR
>Mature_293_residues
TKVTVAATQMACSWDLPKNIENAEKLVRQAHARGAQIILIQELFAAPYFCIDQSPEHYALAQELDNSPLIKHFSKLAAEL
EVVLPLSFFEKANNAYYNSLVMIDADGSVLDVYRKTHIPNGPAYQEKQFFIPGDTGFKVWQTRYAKVGVGICWDQWFPET
ARSLALLGAEVIFYPTAIGSEPAYPEIDSQPHWTRVQQGHAAANLVPVIASNRIGTEKSKYIDGLEMTFYGSSFIADQTG
ALLAQANKTDEAVLVHEFDLQEIAAQRASWGLFRDRRPEMYQTLATSDGKTRR

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=290, Percent_Identity=28.6206896551724, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI5031947, Length=292, Percent_Identity=27.7397260273973, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI297632348, Length=292, Percent_Identity=27.7397260273973, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI297632350, Length=292, Percent_Identity=27.7397260273973, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI7706509, Length=270, Percent_Identity=27.4074074074074, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI297632346, Length=203, Percent_Identity=30.0492610837438, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17533173, Length=270, Percent_Identity=28.8888888888889, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17556280, Length=286, Percent_Identity=24.4755244755245, Blast_Score=78, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6323383, Length=291, Percent_Identity=26.8041237113402, Blast_Score=106, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6322335, Length=306, Percent_Identity=26.797385620915, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21358471, Length=270, Percent_Identity=28.5185185185185, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21355835, Length=288, Percent_Identity=23.9583333333333, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17933642, Length=283, Percent_Identity=23.6749116607774, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 32909; Mature: 32777

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKVTVAATQMACSWDLPKNIENAEKLVRQAHARGAQIILIQELFAAPYFCIDQSPEHYA
CCEEEEEEEHEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEHHHCCCEEEECCCCCHHH
LAQELDNSPLIKHFSKLAAELEVVLPLSFFEKANNAYYNSLVMIDADGSVLDVYRKTHIP
HHHHCCCCHHHHHHHHHHHHHHHEECHHHHHHCCCCEEEEEEEEECCCCEEEHHHHHCCC
NGPAYQEKQFFIPGDTGFKVWQTRYAKVGVGICWDQWFPETARSLALLGAEVIFYPTAIG
CCCCCCCCEEEECCCCCHHHHHHHHHHHCCEEEHHHCCHHHHHHHHHHCCEEEEEEECCC
SEPAYPEIDSQPHWTRVQQGHAAANLVPVIASNRIGTEKSKYIDGLEMTFYGSSFIADQT
CCCCCCCCCCCCCHHHHHCCCCHHHEEEEEECCCCCCCHHHHHCCEEEEEECCCHHCCCC
GALLAQANKTDEAVLVHEFDLQEIAAQRASWGLFRDRRPEMYQTLATSDGKTRR
CHHEEECCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TKVTVAATQMACSWDLPKNIENAEKLVRQAHARGAQIILIQELFAAPYFCIDQSPEHYA
CEEEEEEEHEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEHHHCCCEEEECCCCCHHH
LAQELDNSPLIKHFSKLAAELEVVLPLSFFEKANNAYYNSLVMIDADGSVLDVYRKTHIP
HHHHCCCCHHHHHHHHHHHHHHHEECHHHHHHCCCCEEEEEEEEECCCCEEEHHHHHCCC
NGPAYQEKQFFIPGDTGFKVWQTRYAKVGVGICWDQWFPETARSLALLGAEVIFYPTAIG
CCCCCCCCEEEECCCCCHHHHHHHHHHHCCEEEHHHCCHHHHHHHHHHCCEEEEEEECCC
SEPAYPEIDSQPHWTRVQQGHAAANLVPVIASNRIGTEKSKYIDGLEMTFYGSSFIADQT
CCCCCCCCCCCCCHHHHHCCCCHHHEEEEEECCCCCCCHHHHHCCEEEEEECCCHHCCCC
GALLAQANKTDEAVLVHEFDLQEIAAQRASWGLFRDRRPEMYQTLATSDGKTRR
CHHEEECCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]