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The map label for this gene is bepF [H]

Identifier: 170022987

GI number: 170022987

Start: 796508

End: 797800

Strand: Direct

Name: bepF [H]

Synonym: YPK_0738

Alternate gene names: 170022987

Gene position: 796508-797800 (Clockwise)

Preceding gene: 170022984

Following gene: 170022988

Centisome position: 16.99

GC content: 53.29

Gene sequence:

>1293_bases
ATGTTTACACTGAATTGGGTTCGGTCCACGCCAATTTTTTACGTGCCGCGAACCCTACGCTCTCGGCTAATCTTACGCTC
TCCGCGAATGGGGCCTTTGCGGGCAAAGCAACACCGCCATTCTTTACTGTCCCATGCGCTTTTTCTGCTCATACTCGGGG
CGGGCTCTGTGAGTGCGGCCCCCGCGCCTTTACCTGCCGTAACCGTCGCGGTAGTCGCGTCAATAACACCAGACAATGCC
GTGCAATATCTTGGGCGGATTGAGGCGATTCAAGCGGTTGATGTGACTACCCGCACCGAAGGATTTATCGCCCGCCGTCT
GTTTACTGAAGGGAAAATGGTCAAGCAGGGGGAACTGTTATATGAAATTGATCCCGCTTTGCATCAAGCATCGGTGGCGC
AAGCACAGGCGCAACTCGACAGCGCGACTGCCAGCGCCAATCATGCACAGGTGAACCTCACGCGTTTACAACGCTTGGGT
AATAACCGTTCTGTCAGCCAGGCGGAGGTGGATGAAGCACAGGCACAGCGAGATATCAGCCGGGCAGCGGTGGCGCAGGC
GCAGGCTAACCTACAGATTCAGCAGTTACAGCTCAGCTTCACCCAAATTCATGCGCCAATCAGTGGGCAAATGGGTCACA
GCCGTTTTAATGTGGGCAGCCTGATTAATCCCGCTAGCGGCACCTTGGTCAATATTGTGCAGCTCGATCCGATTCGGGTG
GCGATTGCCGTCAATGAACGGGATTACATTACCGCGACCTATCAACGCGCTGTCGCCCGTGAAAAAACCACTGAAAATCC
CTTGCTCCTTCTGGGGCGTTTTACCCCTCGCATTCAACTGGCAAATGGTAAGCAATATCCAGAGAGTGGTATTTTTGAAT
CGGTGGATAACCAAATTGATAAGCAGACGGGAACGGTCATTATCCGTACTCGTTTTGCCAACCCTCGCCATTTGTTGCTG
CCCGGTGGCGTAGTCAATGTGTCATTGGCTGCAGACGACGCCAAACCTGTGACGGTCATCCCGATTGCTGCTTTACAACA
GAATAAACAGGGCCATTTTGTGCTGGTAGTGACCGATAAAGCGGTGGTCGAAGTGCGCCCAGTGACCCTAGGTGAACAGT
TCGAACAGCAATATGCGGTGACCGATGGGGTGAAGGTGGGTGAAAAAGTCATTGTGGCGGGGCTACAGCATGTCCGACCG
GGTATGGCGGTGAACGCCAGCACGGTTAATGATGGAACTGTAAATGATGGAACTGTAAATGATCGCGTTGCAGCGCCATT
GACGGCTAATTAA

Upstream 100 bases:

>100_bases
TCTTGTTTAACGTGGATCTTCGTTTCATGTGATCTTATTTAATAAGATCAGGTTTAACACCAAGCCGTTCTATCCCGCCG
AGAGTAAGGAATAAATCACC

Downstream 100 bases:

>100_bases
GGGGAAGACCAGATGTTGCATTTCTTTATTCGTCGCCCCAAGTTTGCCATTGTTATCGCATTGGTGATCACCCTGGTTGG
CTGGGTTTCACTGTATGTTA

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MFTLNWVRSTPIFYVPRTLRSRLILRSPRMGPLRAKQHRHSLLSHALFLLILGAGSVSAAPAPLPAVTVAVVASITPDNA
VQYLGRIEAIQAVDVTTRTEGFIARRLFTEGKMVKQGELLYEIDPALHQASVAQAQAQLDSATASANHAQVNLTRLQRLG
NNRSVSQAEVDEAQAQRDISRAAVAQAQANLQIQQLQLSFTQIHAPISGQMGHSRFNVGSLINPASGTLVNIVQLDPIRV
AIAVNERDYITATYQRAVAREKTTENPLLLLGRFTPRIQLANGKQYPESGIFESVDNQIDKQTGTVIIRTRFANPRHLLL
PGGVVNVSLAADDAKPVTVIPIAALQQNKQGHFVLVVTDKAVVEVRPVTLGEQFEQQYAVTDGVKVGEKVIVAGLQHVRP
GMAVNASTVNDGTVNDGTVNDRVAAPLTAN

Sequences:

>Translated_430_residues
MFTLNWVRSTPIFYVPRTLRSRLILRSPRMGPLRAKQHRHSLLSHALFLLILGAGSVSAAPAPLPAVTVAVVASITPDNA
VQYLGRIEAIQAVDVTTRTEGFIARRLFTEGKMVKQGELLYEIDPALHQASVAQAQAQLDSATASANHAQVNLTRLQRLG
NNRSVSQAEVDEAQAQRDISRAAVAQAQANLQIQQLQLSFTQIHAPISGQMGHSRFNVGSLINPASGTLVNIVQLDPIRV
AIAVNERDYITATYQRAVAREKTTENPLLLLGRFTPRIQLANGKQYPESGIFESVDNQIDKQTGTVIIRTRFANPRHLLL
PGGVVNVSLAADDAKPVTVIPIAALQQNKQGHFVLVVTDKAVVEVRPVTLGEQFEQQYAVTDGVKVGEKVIVAGLQHVRP
GMAVNASTVNDGTVNDGTVNDRVAAPLTAN
>Mature_430_residues
MFTLNWVRSTPIFYVPRTLRSRLILRSPRMGPLRAKQHRHSLLSHALFLLILGAGSVSAAPAPLPAVTVAVVASITPDNA
VQYLGRIEAIQAVDVTTRTEGFIARRLFTEGKMVKQGELLYEIDPALHQASVAQAQAQLDSATASANHAQVNLTRLQRLG
NNRSVSQAEVDEAQAQRDISRAAVAQAQANLQIQQLQLSFTQIHAPISGQMGHSRFNVGSLINPASGTLVNIVQLDPIRV
AIAVNERDYITATYQRAVAREKTTENPLLLLGRFTPRIQLANGKQYPESGIFESVDNQIDKQTGTVIIRTRFANPRHLLL
PGGVVNVSLAADDAKPVTVIPIAALQQNKQGHFVLVVTDKAVVEVRPVTLGEQFEQQYAVTDGVKVGEKVIVAGLQHVRP
GMAVNASTVNDGTVNDGTVNDRVAAPLTAN

Specific function: May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789665, Length=349, Percent_Identity=32.9512893982808, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1789929, Length=375, Percent_Identity=29.8666666666667, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1786668, Length=370, Percent_Identity=31.8918918918919, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI87082036, Length=322, Percent_Identity=33.5403726708075, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI87081796, Length=391, Percent_Identity=23.5294117647059, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 46504; Mature: 46504

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTLNWVRSTPIFYVPRTLRSRLILRSPRMGPLRAKQHRHSLLSHALFLLILGAGSVSAA
CEEEEEECCCCEEEECHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
PAPLPAVTVAVVASITPDNAVQYLGRIEAIQAVDVTTRTEGFIARRLFTEGKMVKQGELL
CCCCCCEEEEHEEECCCHHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHCCCEEECCCEE
YEIDPALHQASVAQAQAQLDSATASANHAQVNLTRLQRLGNNRSVSQAEVDEAQAQRDIS
EEECHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHCCCCCCCCHHHHHHHHHHHHHH
RAAVAQAQANLQIQQLQLSFTQIHAPISGQMGHSRFNVGSLINPASGTLVNIVQLDPIRV
HHHHHHHHCCCEEEHEEEEHHHEECCCCCCCCCCEECCCCCCCCCCCCEEEEEEECCEEE
AIAVNERDYITATYQRAVAREKTTENPLLLLGRFTPRIQLANGKQYPESGIFESVDNQID
EEEECCCCEEHHHHHHHHHHHCCCCCCEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHH
KQTGTVIIRTRFANPRHLLLPGGVVNVSLAADDAKPVTVIPIAALQQNKQGHFVLVVTDK
HCCCEEEEEEECCCCCEEEECCCEEEEEEECCCCCCEEEEEEHHHCCCCCCCEEEEEECC
AVVEVRPVTLGEQFEQQYAVTDGVKVGEKVIVAGLQHVRPGMAVNASTVNDGTVNDGTVN
EEEEEECCCCCHHHHHHHHHHCCCHHCHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCC
DRVAAPLTAN
CCCCCCCCCC
>Mature Secondary Structure
MFTLNWVRSTPIFYVPRTLRSRLILRSPRMGPLRAKQHRHSLLSHALFLLILGAGSVSAA
CEEEEEECCCCEEEECHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
PAPLPAVTVAVVASITPDNAVQYLGRIEAIQAVDVTTRTEGFIARRLFTEGKMVKQGELL
CCCCCCEEEEHEEECCCHHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHCCCEEECCCEE
YEIDPALHQASVAQAQAQLDSATASANHAQVNLTRLQRLGNNRSVSQAEVDEAQAQRDIS
EEECHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHCCCCCCCCHHHHHHHHHHHHHH
RAAVAQAQANLQIQQLQLSFTQIHAPISGQMGHSRFNVGSLINPASGTLVNIVQLDPIRV
HHHHHHHHCCCEEEHEEEEHHHEECCCCCCCCCCEECCCCCCCCCCCCEEEEEEECCEEE
AIAVNERDYITATYQRAVAREKTTENPLLLLGRFTPRIQLANGKQYPESGIFESVDNQID
EEEECCCCEEHHHHHHHHHHHCCCCCCEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHH
KQTGTVIIRTRFANPRHLLLPGGVVNVSLAADDAKPVTVIPIAALQQNKQGHFVLVVTDK
HCCCEEEEEEECCCCCEEEECCCEEEEEEECCCCCCEEEEEEHHHCCCCCCCEEEEEECC
AVVEVRPVTLGEQFEQQYAVTDGVKVGEKVIVAGLQHVRPGMAVNASTVNDGTVNDGTVN
EEEEEECCCCCHHHHHHHHHHCCCHHCHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCC
DRVAAPLTAN
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]