Click here to switch to the map view.

The map label for this gene is greB

Identifier: 170022432

GI number: 170022432

Start: 222609

End: 223124

Strand: Direct

Name: greB

Synonym: YPK_0171

Alternate gene names: 170022432

Gene position: 222609-223124 (Clockwise)

Preceding gene: 170022427

Following gene: 170022433

Centisome position: 4.75

GC content: 49.03

Gene sequence:

>516_bases
ATGGCAAAAAGTAACTACATTACCCGCGAAGGCTGGCAGGCGCTGGACCGTGAATTGCATTATCTGTGGCGAGAAGAGCG
CCCGGTCGTCACTCAGGCGGTTTCTGAAGCCGCTGCTATGGGTGACCGTTCAGAAAATGCTGAATATATTTATGGAAAGA
AGCGGCTGAGAGAAATCGATCGCCGGGTACGCTTTCTCGCCAAGCGCCTTGAAGTGCTGAAAATTGTTGACCCCGATCCT
CGCCAAGAAGGTAAAGTCTTTTTTGGGGCCTGGGTGCGGGTGGAAAATGAGCAAGAAGAGCAACGCATCTTCCGTTTGGT
GGGGCCAGATGAGTTTGACCCGGCCAAAAAATGGATTTCAATTGATTCACCGGTCGCCCGTGCATTGATAGGGAAACAAG
TCGATGACGAGGTCACCGTGCAAACGCCTAATGGTGAGGCAACCTATTGGATATTAGAGATTCGTTATCGGCCATTTGAT
GAAGATAGGCCATTTAATGAAGATATTGATAATTAG

Upstream 100 bases:

>100_bases
AGTTATTATAATGAAAAATGGCTAACTTGATGAAAAATAACGATTATGGTGAAGATAACAACGATGATAAAAATAGCGAC
GATGGCAAAAAGTAACGATT

Downstream 100 bases:

>100_bases
GCGTTTGATAATTAAGCATACGAATAAGCTTTGTAACAATTTCAACTAGAATTTATACCATTAATAACTGTCTGTTAGAG
TAAGTTTTTCTAACAATGTG

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB

Number of amino acids: Translated: 171; Mature: 170

Protein sequence:

>171_residues
MAKSNYITREGWQALDRELHYLWREERPVVTQAVSEAAAMGDRSENAEYIYGKKRLREIDRRVRFLAKRLEVLKIVDPDP
RQEGKVFFGAWVRVENEQEEQRIFRLVGPDEFDPAKKWISIDSPVARALIGKQVDDEVTVQTPNGEATYWILEIRYRPFD
EDRPFNEDIDN

Sequences:

>Translated_171_residues
MAKSNYITREGWQALDRELHYLWREERPVVTQAVSEAAAMGDRSENAEYIYGKKRLREIDRRVRFLAKRLEVLKIVDPDP
RQEGKVFFGAWVRVENEQEEQRIFRLVGPDEFDPAKKWISIDSPVARALIGKQVDDEVTVQTPNGEATYWILEIRYRPFD
EDRPFNEDIDN
>Mature_170_residues
AKSNYITREGWQALDRELHYLWREERPVVTQAVSEAAAMGDRSENAEYIYGKKRLREIDRRVRFLAKRLEVLKIVDPDPR
QEGKVFFGAWVRVENEQEEQRIFRLVGPDEFDPAKKWISIDSPVARALIGKQVDDEVTVQTPNGEATYWILEIRYRPFDE
DRPFNEDIDN

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082261, Length=154, Percent_Identity=60.3896103896104, Blast_Score=182, Evalue=1e-47,
Organism=Escherichia coli, GI87082225, Length=152, Percent_Identity=35.5263157894737, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GREB_YERPE (Q8ZJH2)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AD0017
- RefSeq:   NP_671208.1
- RefSeq:   NP_991537.1
- RefSeq:   YP_002345221.1
- ProteinModelPortal:   Q8ZJH2
- SMR:   Q8ZJH2
- GeneID:   1148862
- GeneID:   1172981
- GeneID:   2766812
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO0135
- KEGG:   ypk:y3915
- KEGG:   ypm:YP_0136
- HOGENOM:   HBG753387
- OMA:   KRFRIVG
- ProtClustDB:   PRK01885
- BioCyc:   YPES187410:Y3915-MONOMER
- BioCyc:   YPES214092:YPO0135-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01461

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 20214; Mature: 20083

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSNYITREGWQALDRELHYLWREERPVVTQAVSEAAAMGDRSENAEYIYGKKRLREID
CCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHH
RRVRFLAKRLEVLKIVDPDPRQEGKVFFGAWVRVENEQEEQRIFRLVGPDEFDPAKKWIS
HHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHCCCCCCCCHHHHCC
IDSPVARALIGKQVDDEVTVQTPNGEATYWILEIRYRPFDEDRPFNEDIDN
CCCHHHHHHHCCCCCCCEEEECCCCCEEEEEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKSNYITREGWQALDRELHYLWREERPVVTQAVSEAAAMGDRSENAEYIYGKKRLREID
CCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHH
RRVRFLAKRLEVLKIVDPDPRQEGKVFFGAWVRVENEQEEQRIFRLVGPDEFDPAKKWIS
HHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHCCCCCCCCHHHHCC
IDSPVARALIGKQVDDEVTVQTPNGEATYWILEIRYRPFDEDRPFNEDIDN
CCCHHHHHHHCCCCCCCEEEECCCCCEEEEEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430