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The map label for this gene is leuS [H]

Identifier: 169834477

GI number: 169834477

Start: 316248

End: 318749

Strand: Direct

Name: leuS [H]

Synonym: SPH_0372

Alternate gene names: 169834477

Gene position: 316248-318749 (Clockwise)

Preceding gene: 169833783

Following gene: 169834287

Centisome position: 14.08

GC content: 44.64

Gene sequence:

>2502_bases
ATGAGTTTTTACAATCATAAAGAAATTGAGCCTAAGTGGCAGGGCTACTGGGCAGAACATCATACATTTAAGACAGGAAC
AGATGCATCAAAACCTAAGTTTTATGCGCTTGATATGTTCCCTTATCCGTCTGGAGCTGGTCTGCACGTAGGACACCCAG
AAGGTTATACTGCAACCGATATCCTCAGTCGTTACAAACGTGCGCAAGGCTACAATGTTCTTCACCCAATGGGTTGGGAT
GCTTTTGGTTTGCCTGCAGAGCAATACGCTATGGATACTGGTAATGACCCAGCAGAATTTACAGCGGAAAACATTGCCAA
CTTCAAACGTCAAATCAATGCGCTTGGATTTTCTTATGACTGGGATCGTGAAGTCAATACAACCGATCCAAACTACTACA
AGTGGACGCAATGGATTTTCACTAAGCTTTATGAAAAAGGTTTGGCCTATGAAGCTGAAGTGCCAGTAAACTGGGTTGAG
GAATTGGGAACAGCCATCGCTAACGAAGAAGTTCTTCCTGACGGAACCTCTGAGCGTGGGGGCTATCCAGTTGTCCGCAA
ACCAATGCGCCAATGGATGCTCAAAATCACGGCCTACGCAGAGCGCTTGCTCAATGACTTAGATGAACTAGATTGGCCAG
AGTCTATCAAGGACATGCAACGCAACTGGATTGGTAAATCAACTGGTGCCAATGTAACTTTTAAGGTTAAGGGAACAGAC
AAGGAATTTACAGTCTTTACTACTCGTCCTGATACTCTTTTCGGTGCGACTTTCACTGTTTTGGCTCCTGAACATGAATT
AGTAGATGCCATCACAAGCACAGAGCAAGCAGAGGCAGTTGCAGACTATAAACACCAAGCTAGCCTCAAATCTGACTTGG
CTCGTACAGACCTTGCCAAAGAAAAAACTGGTGTCTGGACAGGTGCTTATGCCATCAATCCTGTCAATGGTAAGGAAATT
CCAATCTGGATTGCGGATTACGTTCTTGCTAGCTATGGTACAGGTGCGGTAATGGCTGTGCCTGCCCACGATCAACGTGA
CTGGGAATTTGCCAAACAATTTGACCTTCCAATCGTCGAAGTACTTGAAGGTGGAAATGTTGAAGAAGCTGCCTACACAG
AAGATGGCCTTCACGTTAATTCAGACTTCCTAGATGGACTCAACAAAGAAGACGCTATTGCCAAGATTGTGGCTTGGTTG
GAAGAAAAAGGCTGTGGACAAGAGAAGGTAACCTACCGTCTCCGCGACTGGCTCTTTAGCCGTCAACGTTACTGGGGTGA
GCCAATCCCAATCATTCATTGGGAAGATGGAACTTCAACAGCTGTCCCTGAAAGTGAATTGCCACTTGTCTTGCCTGTAA
CCAAGGATATCCGTCCCTCAGGTACTGGTGAAAGTCCACTAGCTAACTTGACAGATTGGCTTGAAGTGACTCGTGAAGAT
GGTGTCAAAGGTCGTCGTGAAACCAACACTATGCCACAATGGGCTGGTTCAAGCTGGTACTACCTCCGCTATATTGATCC
ACACAATACTGAGAAGTTGGCTGATGAGGACCTCCTCAAACAATGGTTGCCAGTAGATATCTACGTGGGTGGTGCAGAGC
ATGCTGTTCTTCACTTGCTTTACGCTCGTTTCTGGCACAAATTCCTCTATGACCTCGGTGTTGTTCCGACTAAGGAACCA
TTCCAAAAACTCTTTAACCAAGGGATGATTTTGGGAACAAGCTACCGTGACCACCGTGGTGCTCTTGTGGCAACCGACAA
GGTTGAAAAACGTGATGGTTCCTTCTTCCATGTAGAAACAGGGGAAGAGTTGGAGCAAGCGCCAGCCAAGATGTCTAAAT
CGCTCAAGAACGTTGTTAACCCAGACGATGTGGTGGAACAATACGGTGCCGATACCCTTCGTGTTTATGAAATGTTTATG
GGACCACTCGATGCTTCGATTGCTTGGTCAGAAGAAGGTTTGGAAGGAAGCCGTAAGTTCCTTGACCGAGTTTACCGTTT
GATTACAAGTAAAGAAATCCTTGCGGAAAACAATGGTGCTCTTGACAAGGTTTACAACGAAACAGTCAAAGCTGTTACTG
AGCAAATTGAGTCTCTCAAATTCAACACAGCTATTGCCCAACTTATGGTCTTTGTCAATGCTGCTAACAAGGAAGATAAG
CTTTATGTTGACTATGCCAAAGGCTTTATTCAATTGATTGCACCATTTGCACCTCACTTGGCAGAAGAACTCTGGCAAAC
AGTCGCAGAAACAGGTGAGTCAATCTCTTATGTAGCTTGGCCAACTTGGGACGAAAGCAAATTGGTTGAAGATGAAATTG
AAATTGTCGTCCAAATCAAAGGAAAAGTTCGTGCCAAACTCATGGTTGCTAAAGATCTATCACGTGAAGAATTACAAGAA
ATCGCTTTAGCTGATGAAAAAGTCAAAGCAGAAATTGACGGTAAGGAAATCGTGAAAGTAATTGCGGTACCGAATAAACT
CGTTAATATCGTCGTTAAATAA

Upstream 100 bases:

>100_bases
TCAAAATTAATGAGATACAGTATACAGTTTTTCCTTTAAACACATTTCAAATTCCCTCAAAAATGGTATAATAGTAACAT
CACAAAATTGGAGAGAGACC

Downstream 100 bases:

>100_bases
CGAGTTTATTAGCTCTATCTGCCACCTTCAATAGTCCACTGGACTATTGAAGCCAACTAAATTAGTTAACATTGTTGTGA
AATAAAAAGAAGTAACTCTA

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 833; Mature: 832

Protein sequence:

>833_residues
MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPSGAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWD
AFGLPAEQYAMDTGNDPAEFTAENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWPESIKDMQRNWIGKSTGANVTFKVKGTD
KEFTVFTTRPDTLFGATFTVLAPEHELVDAITSTEQAEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEI
PIWIADYVLASYGTGAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDAIAKIVAWL
EEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLVLPVTKDIRPSGTGESPLANLTDWLEVTRED
GVKGRRETNTMPQWAGSSWYYLRYIDPHNTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEP
FQKLFNQGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDVVEQYGADTLRVYEMFM
GPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALDKVYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDK
LYVDYAKGFIQLIAPFAPHLAEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREELQE
IALADEKVKAEIDGKEIVKVIAVPNKLVNIVVK

Sequences:

>Translated_833_residues
MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPSGAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWD
AFGLPAEQYAMDTGNDPAEFTAENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWPESIKDMQRNWIGKSTGANVTFKVKGTD
KEFTVFTTRPDTLFGATFTVLAPEHELVDAITSTEQAEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEI
PIWIADYVLASYGTGAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDAIAKIVAWL
EEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLVLPVTKDIRPSGTGESPLANLTDWLEVTRED
GVKGRRETNTMPQWAGSSWYYLRYIDPHNTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEP
FQKLFNQGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDVVEQYGADTLRVYEMFM
GPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALDKVYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDK
LYVDYAKGFIQLIAPFAPHLAEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREELQE
IALADEKVKAEIDGKEIVKVIAVPNKLVNIVVK
>Mature_832_residues
SFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPSGAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDA
FGLPAEQYAMDTGNDPAEFTAENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVEE
LGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWPESIKDMQRNWIGKSTGANVTFKVKGTDK
EFTVFTTRPDTLFGATFTVLAPEHELVDAITSTEQAEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEIP
IWIADYVLASYGTGAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDAIAKIVAWLE
EKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLVLPVTKDIRPSGTGESPLANLTDWLEVTREDG
VKGRRETNTMPQWAGSSWYYLRYIDPHNTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPF
QKLFNQGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDVVEQYGADTLRVYEMFMG
PLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALDKVYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKL
YVDYAKGFIQLIAPFAPHLAEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREELQEI
ALADEKVKAEIDGKEIVKVIAVPNKLVNIVVK

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=881, Percent_Identity=33.4846765039728, Blast_Score=486, Evalue=1e-137,
Organism=Homo sapiens, GI94721239, Length=181, Percent_Identity=28.7292817679558, Blast_Score=76, Evalue=2e-13,
Organism=Homo sapiens, GI94721241, Length=181, Percent_Identity=28.7292817679558, Blast_Score=76, Evalue=2e-13,
Organism=Escherichia coli, GI1786861, Length=866, Percent_Identity=42.9561200923788, Blast_Score=667, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=398, Percent_Identity=27.1356783919598, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71997517, Length=775, Percent_Identity=31.0967741935484, Blast_Score=354, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI71997510, Length=775, Percent_Identity=31.0967741935484, Blast_Score=354, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI212645227, Length=372, Percent_Identity=30.3763440860215, Blast_Score=162, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI71980946, Length=150, Percent_Identity=32.6666666666667, Blast_Score=68, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323414, Length=860, Percent_Identity=35.6976744186046, Blast_Score=471, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6321531, Length=434, Percent_Identity=27.1889400921659, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6319395, Length=180, Percent_Identity=28.3333333333333, Blast_Score=68, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6325217, Length=159, Percent_Identity=31.4465408805031, Blast_Score=64, Evalue=1e-10,
Organism=Drosophila melanogaster, GI21355409, Length=750, Percent_Identity=34.1333333333333, Blast_Score=397, Evalue=1e-110,
Organism=Drosophila melanogaster, GI17864482, Length=456, Percent_Identity=23.9035087719298, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24653289, Length=456, Percent_Identity=23.9035087719298, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI281366294, Length=375, Percent_Identity=25.0666666666667, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 94384; Mature: 94253

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPSGAGLHVGHPEGYTATD
CCCCCCCCCCCHHCCEEHHCCCCCCCCCCCCCEEEEEEECCCCCCCCEECCCCCCCCHHH
ILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTAENIANFKRQINALGFSYD
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
WDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVEELGTAIANEEVLPDGTSERG
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHCCCCCCCCCCCCCC
GYPVVRKPMRQWMLKITAYAERLLNDLDELDWPESIKDMQRNWIGKSTGANVTFKVKGTD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEEECCC
KEFTVFTTRPDTLFGATFTVLAPEHELVDAITSTEQAEAVADYKHQASLKSDLARTDLAK
CEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKTGVWTGAYAINPVNGKEIPIWIADYVLASYGTGAVMAVPAHDQRDWEFAKQFDLPIVE
HHCCCEECCEEECCCCCCCCEEEHHHHHHHHCCCCCEEEECCCCCCCHHHHHHCCCCHHH
VLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDAIAKIVAWLEEKGCGQEKVTYRLRDWLFS
HHCCCCCCHHHCCCCCCEECHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
RQRYWGEPIPIIHWEDGTSTAVPESELPLVLPVTKDIRPSGTGESPLANLTDWLEVTRED
HHHHCCCCCCEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHC
GVKGRRETNTMPQWAGSSWYYLRYIDPHNTEKLADEDLLKQWLPVDIYVGGAEHAVLHLL
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHH
YARFWHKFLYDLGVVPTKEPFQKLFNQGMILGTSYRDHRGALVATDKVEKRDGSFFHVET
HHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEECCCCCCCCCEEEECCHHCCCCCEEEEEC
GEELEQAPAKMSKSLKNVVNPDDVVEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKF
CHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHCCCCCCEEECCCCCCHHHHH
LDRVYRLITSKEILAENNGALDKVYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDK
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
LYVDYAKGFIQLIAPFAPHLAEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIK
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHEEEEEEEC
GKVRAKLMVAKDLSREELQEIALADEKVKAEIDGKEIVKVIAVPNKLVNIVVK
CCCEEEEHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHEEEECC
>Mature Secondary Structure 
SFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPSGAGLHVGHPEGYTATD
CCCCCCCCCCHHCCEEHHCCCCCCCCCCCCCEEEEEEECCCCCCCCEECCCCCCCCHHH
ILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTAENIANFKRQINALGFSYD
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
WDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVEELGTAIANEEVLPDGTSERG
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHCCCCCCCCCCCCCC
GYPVVRKPMRQWMLKITAYAERLLNDLDELDWPESIKDMQRNWIGKSTGANVTFKVKGTD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEEECCC
KEFTVFTTRPDTLFGATFTVLAPEHELVDAITSTEQAEAVADYKHQASLKSDLARTDLAK
CEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKTGVWTGAYAINPVNGKEIPIWIADYVLASYGTGAVMAVPAHDQRDWEFAKQFDLPIVE
HHCCCEECCEEECCCCCCCCEEEHHHHHHHHCCCCCEEEECCCCCCCHHHHHHCCCCHHH
VLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDAIAKIVAWLEEKGCGQEKVTYRLRDWLFS
HHCCCCCCHHHCCCCCCEECHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
RQRYWGEPIPIIHWEDGTSTAVPESELPLVLPVTKDIRPSGTGESPLANLTDWLEVTRED
HHHHCCCCCCEEEECCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHC
GVKGRRETNTMPQWAGSSWYYLRYIDPHNTEKLADEDLLKQWLPVDIYVGGAEHAVLHLL
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHH
YARFWHKFLYDLGVVPTKEPFQKLFNQGMILGTSYRDHRGALVATDKVEKRDGSFFHVET
HHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEECCCCCCCCCEEEECCHHCCCCCEEEEEC
GEELEQAPAKMSKSLKNVVNPDDVVEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKF
CHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHCCCCCCEEECCCCCCHHHHH
LDRVYRLITSKEILAENNGALDKVYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDK
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
LYVDYAKGFIQLIAPFAPHLAEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIK
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHEEEEEEEC
GKVRAKLMVAKDLSREELQEIALADEKVKAEIDGKEIVKVIAVPNKLVNIVVK
CCCEEEEHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA