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The map label for this gene is thrS [H]

Identifier: 169834472

GI number: 169834472

Start: 1603699

End: 1605642

Strand: Direct

Name: thrS [H]

Synonym: SPH_1742

Alternate gene names: 169834472

Gene position: 1603699-1605642 (Clockwise)

Preceding gene: 169833926

Following gene: 169833574

Centisome position: 71.41

GC content: 45.42

Gene sequence:

>1944_bases
ATGATTAACATTACTTTCCCAGATGGCGCTGTTCGTGAATTCGAATCTGGCGTAACAACTTTTGAAATTGCCCAATCTAT
CAGCAATTCCCTAGCTAAAAAAGCCTTGGCTGGTAAATTCAACGGCAAACTCATCGACACTACTCGCGCTATCACTGAAG
ATGGAAGCATCGAAATTGTGACACCTGATCACGAAGATGCCCTTCCAATCTTGCGTCACTCAGCAGCTCACTTGTTCGCC
CAAGCAGCTCGTCGTCTTTTCCCAGACATTCACTTGGGAGTTGGTCCAGCCATCGAAGATGGTTTCTACTACGATACTGA
CAACACAGCTGGTCAAATCTCTAACGAAGACCTTCCTCGTATCGAAGAAGAAATGCAAAAAATCGTCAAAGAAAACTTCC
CATCTATTCGTGAAGAAGTGACTAAAGACGAGGCACGTGAAATCTTCAAAAATGACCCTTACAAGTTGGAATTGATTGAA
GAACACTCAGAAGACGAAGGCGGTTTGACTATCTATCGTCAGGGTGAATATGTAGACCTCTGCCGTGGACCTCACGTTCC
ATCAACAGGTCGTATCCAAATCTTCCACCTTCTCCATGTAGCTGGTGCGTACTGGCGTGGAAACAGCGACAACGCTATGA
TGCAACGTATCTACGGTACAGCTTGGTTTGACAAGAAAGACTTGAAAAACTACCTTCAAATGCGTGAAGAAGCTAAGGAA
CGTGACCACCGTAAACTTGGTAAAGAGCTTGACCTCTTTATGATTTCACAAGAAGTGGGACAAGGTTTGCCATTCTGGTT
GCCAAATGGTGCGACTATCCGTCGTGAATTGGAACGCTACATCGTAAACAAAGAGTTGGCTTCTGGCTACCAACACGTCT
ACACTCCACCACTTGCTTCTGTTGAGCTTTACAAGACTTCTGGTCACTGGGATCATTACCAAGAAGACATGTTCCCAACC
ATGGACATGGGTGACGGGGAAGAATTTGTCCTTCGTCCAATGAACTGTCCGCACCACATCCAAGTTTTCAAACACCATGT
TCACTCTTACCGTGAATTGCCAATCCGTATCGCTGAAATCGGTATGATGCACCGTTACGAAAAATCTGGTGCCCTCACTG
GCCTTCAACGTGTACGTGAAATGTCACTCAACGACGGTCACCTATTCGTTACTCCAGAACAAATCCAAGAAGAATTCCAA
CGTGCCCTTCAGTTGATTATCGATGTTTATGAAGACTTCAACTTGACTGACTACCGCTTCCGCCTCTCTCTTCGTGACCC
TCAAGATACTCATAAGTACTTTGATAACGATGAGATGTGGGAAAATGCCCAAACCATGCTTCGTGCAGCTCTTGATGAAA
TGGGCGTGGACTACTTTGAAGCCGAAGGTGAAGCAGCCTTCTACGGACCAAAATTGGATATCCAGATTAAAACTGCCCTT
GGAAAAGAAGAAACCCTTTCTACTATCCAACTTGATTTCTTGTTGCCAGAACGCTTCGACCTCAAATACATCGGAGCTGA
TGGCGAAGATCACCGTCCAGTCATGATCCACCGTGGGGTTATCTCAACTATGGAACGCTTCACAGCTATCTTGATTGAGA
ACTACAAGGGGGCCTTCCCAACATGGCTGGCACCACACCAAGTAACCCTCATCCCAGTATCTAACGAAAAACACGTGGAC
TACGCTTGGGAAGTGGCCAAGAAACTCCGTGACCGCGGTGTCCGTGCAGACGTAGATGAGCGCAATGAAAAAATGCAGTT
CAAGATTCGTGCTTCACAAACCAGCAAGATTCCTTACCAATTAATTGTTGGAGACAAAGAAATGGAAGACGAAACAGTCA
ACGTTCGTCGCTACGGCCAAAAAGAAACACAAACTGTCTCAGTTGATAATTTTGTTCAAGCTATCCTAGCTGATATCGCC
AACAAATCACGCGTTGAGAAATAA

Upstream 100 bases:

>100_bases
CTCTGATAAAGAAAAGCAATTGAACGTTAGGACTTGGAAGTAGTCTGCTGATAAAAAATCAATGCTTAGAAACTATGAAA
TAATAAAAAAGGAGAACATC

Downstream 100 bases:

>100_bases
GAGTCTAGCATAAAAGCCTCCAATCTGGAGGCTTTTTCTCATCTATTTTTACTCAAGGACTAAGTTCACTTGAGCAAACT
GAATCCGCACTGTCGTTCCT

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 647; Mature: 647

Protein sequence:

>647_residues
MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKLIDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFA
QAARRLFPDIHLGVGPAIEDGFYYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIYGTAWFDKKDLKNYLQMREEAKE
RDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRELERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPT
MDMGDGEEFVLRPMNCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTPEQIQEEFQ
RALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAALDEMGVDYFEAEGEAAFYGPKLDIQIKTAL
GKEETLSTIQLDFLLPERFDLKYIGADGEDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVD
YAWEVAKKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQTVSVDNFVQAILADIA
NKSRVEK

Sequences:

>Translated_647_residues
MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKLIDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFA
QAARRLFPDIHLGVGPAIEDGFYYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIYGTAWFDKKDLKNYLQMREEAKE
RDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRELERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPT
MDMGDGEEFVLRPMNCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTPEQIQEEFQ
RALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAALDEMGVDYFEAEGEAAFYGPKLDIQIKTAL
GKEETLSTIQLDFLLPERFDLKYIGADGEDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVD
YAWEVAKKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQTVSVDNFVQAILADIA
NKSRVEK
>Mature_647_residues
MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKLIDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFA
QAARRLFPDIHLGVGPAIEDGFYYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIYGTAWFDKKDLKNYLQMREEAKE
RDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRELERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPT
MDMGDGEEFVLRPMNCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTPEQIQEEFQ
RALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAALDEMGVDYFEAEGEAAFYGPKLDIQIKTAL
GKEETLSTIQLDFLLPERFDLKYIGADGEDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVD
YAWEVAKKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQTVSVDNFVQAILADIA
NKSRVEK

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38570111, Length=621, Percent_Identity=39.9355877616747, Blast_Score=452, Evalue=1e-127,
Organism=Homo sapiens, GI38202255, Length=618, Percent_Identity=39.8058252427184, Blast_Score=452, Evalue=1e-127,
Organism=Homo sapiens, GI20070344, Length=633, Percent_Identity=36.0189573459716, Blast_Score=398, Evalue=1e-111,
Organism=Escherichia coli, GI1788013, Length=642, Percent_Identity=41.4330218068536, Blast_Score=533, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI71984192, Length=633, Percent_Identity=39.9684044233807, Blast_Score=462, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI71984184, Length=633, Percent_Identity=39.9684044233807, Blast_Score=462, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6322112, Length=658, Percent_Identity=37.0820668693009, Blast_Score=436, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6322654, Length=394, Percent_Identity=37.0558375634518, Blast_Score=258, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24583839, Length=636, Percent_Identity=38.9937106918239, Blast_Score=457, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24583842, Length=636, Percent_Identity=38.9937106918239, Blast_Score=457, Evalue=1e-128,
Organism=Drosophila melanogaster, GI28574141, Length=636, Percent_Identity=38.9937106918239, Blast_Score=457, Evalue=1e-128,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 74684; Mature: 74684

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKLIDTTRAITEDGSIEIV
CEEEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHCCCCCEEEE
TPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGFYYDTDNTAGQISNEDLPR
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHH
IEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIEEHSEDEGGLTIYRQGEYVDL
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEECCCEEEE
CRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIYGTAWFDKKDLKNYLQMREEAKE
CCCCCCCCCCCEEHEEHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
RDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRELERYIVNKELASGYQHVYTPPLAS
HHHHHHCCHHHHEEEEHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCC
VELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPMNCPHHIQVFKHHVHSYRELPIRIAEI
EEEEECCCCCCHHHHHCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHH
GMMHRYEKSGALTGLQRVREMSLNDGHLFVTPEQIQEEFQRALQLIIDVYEDFNLTDYRF
HHHHHHHHCCHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
RLSLRDPQDTHKYFDNDEMWENAQTMLRAALDEMGVDYFEAEGEAAFYGPKLDIQIKTAL
EEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEECCEEEEEEEECC
GKEETLSTIQLDFLLPERFDLKYIGADGEDHRPVMIHRGVISTMERFTAILIENYKGAFP
CCCHHHHEEEEEEECCCCCCEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCC
TWLAPHQVTLIPVSNEKHVDYAWEVAKKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQ
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCEE
LIVGDKEMEDETVNVRRYGQKETQTVSVDNFVQAILADIANKSRVEK
EEECCCCCCCCHHHHHHCCCCCCCEECHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKLIDTTRAITEDGSIEIV
CEEEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHCCCCCEEEE
TPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGFYYDTDNTAGQISNEDLPR
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHH
IEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIEEHSEDEGGLTIYRQGEYVDL
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEECCCEEEE
CRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIYGTAWFDKKDLKNYLQMREEAKE
CCCCCCCCCCCEEHEEHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
RDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRELERYIVNKELASGYQHVYTPPLAS
HHHHHHCCHHHHEEEEHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCC
VELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPMNCPHHIQVFKHHVHSYRELPIRIAEI
EEEEECCCCCCHHHHHCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHH
GMMHRYEKSGALTGLQRVREMSLNDGHLFVTPEQIQEEFQRALQLIIDVYEDFNLTDYRF
HHHHHHHHCCHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
RLSLRDPQDTHKYFDNDEMWENAQTMLRAALDEMGVDYFEAEGEAAFYGPKLDIQIKTAL
EEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEECCEEEEEEEECC
GKEETLSTIQLDFLLPERFDLKYIGADGEDHRPVMIHRGVISTMERFTAILIENYKGAFP
CCCHHHHEEEEEEECCCCCCEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCC
TWLAPHQVTLIPVSNEKHVDYAWEVAKKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQ
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCEE
LIVGDKEMEDETVNVRRYGQKETQTVSVDNFVQAILADIANKSRVEK
EEECCCCCCCCHHHHHHCCCCCCCEECHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA