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The map label for this gene is cysS [H]

Identifier: 169834403

GI number: 169834403

Start: 636292

End: 637665

Strand: Direct

Name: cysS [H]

Synonym: SPH_0689

Alternate gene names: 169834403

Gene position: 636292-637665 (Clockwise)

Preceding gene: 169833934

Following gene: 169834221

Centisome position: 28.33

GC content: 44.76

Gene sequence:

>1374_bases
TTGGATTTTGGCTTTCCTGACTATTATTTTATGATTAAAATCTATGACACTATGTCTCGTGATTTGCGAGAATTTGTCCC
GATTGAGGACGGCAAAGTCAAGATGTATGTTTGTGGGCCAACCGTGTATAACTATATCCACGTGGGGAATGCTCGTTCGA
CGGTAGCTTTTGATACCATTCGTCGCTATTTTGAGTACCGTGGGTATGAGGTTGCCTATATTTCCAATTTTACAGATGTG
GATGATAAGATTATCAACCGTGCCAAGGAAGAGGGCATCACGCCTCAGGAGGTTGCGGACAAGTACATCGCTGCCTTTCG
TGAGGATGTGACGGCCTTGGGCGTGAAACCTGCGACTCGCCATCCGCGTGTGGTGGAGTTTATGGATGACATTATCCGCT
TTGTGGAAGATTTGATTGAAAAAGGCTTTGCCTACGAGAGTCAAGGGGATGTTTACTTCCGTGTGGAAAAATCCCACAAC
TATGCCAAGTTGGCTAACAAAACCTTGGAAGATTTGGAGCTGGGTGCTTCAGGTCGTACCGATGAAGAAACAGCTCGTAA
GGAAAATCCTGTAGACTTTGCCCTCTGGAAATCTGCCAAACCAGGCGAGATTTCTTGGGACAGTCCTTGGGGACCTGGAC
GTCCGGGTTGGCATATTGAGTGTTCAGTCATGTCGACAGAGATTTTAGGTGATACCATTGATATCCACGGTGGTGGAGCT
GACCTAGAGTTTCCACACCACACCAATGAAATTGCCCAGTCTGAAGCTAAAACAGGCAAGGCTTTTGCTAACTACTGGAT
GCACAATGGCTTTGTCAATATCGACAATGTCAAAATGTCTAAGTCCTTGGGTAACTTCATTACGGTACACGATGCCCTTA
AAACTCTTGATGGGCAAGTACTTCGTTTCTTCTTTGCGACCCAGCACTACCGTAAGCCTATCAACTTCACGGAAAAGGCA
GTTCGCGATGCCGAGACCAATCTCAAGTATCTGAAGAATACTTACGAACAACCATTTACAGGAAATGTGGATGCCCAAGA
GTTACAAAACTTTAAAGATAAGTTTGTAGCAGCTATGGATGAAGATTTTAATGCTGCCAACGGTATCACAGTTGTCTTTG
AAATGGCCAAATGGATCAACTCAGGGAACTATGATGCAAGTGTCAAGCAAGCTCTTGCAGATATGTTAGAAATTTTTGGA
ATTGTCTTTGTTGAGGAAGTTTTGGATGCAGAGATTGAAGACTTGATTCAAAAACGCCAAGAGGCGCGTGCCAATCGTGA
CTTTGCGACAGCAGACCAAATCCGTGACCAATTGGTTACTCAAGGAATTAAGCTCCTTGATACCAAGGATGGAGTGAGGT
GGACACGTGATTGA

Upstream 100 bases:

>100_bases
CAGGTGGTTTATCTGAATGAGAAAGGAGCAAGGGATTCCGAAGTGTAGAGACATTCGGACTGAAATTCAATTGAACTCTT
AGTGATGAAATTAACTGTTC

Downstream 100 bases:

>100_bases
TGTCAATCTCATTAACGGGATTGCGCTAGCCTTTGAGGGGGATGCGGTGTATTCTATGTATATTCGCCGTCACCTCATCC
TCAAAGGTATGACCAAACCC

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MDFGFPDYYFMIKIYDTMSRDLREFVPIEDGKVKMYVCGPTVYNYIHVGNARSTVAFDTIRRYFEYRGYEVAYISNFTDV
DDKIINRAKEEGITPQEVADKYIAAFREDVTALGVKPATRHPRVVEFMDDIIRFVEDLIEKGFAYESQGDVYFRVEKSHN
YAKLANKTLEDLELGASGRTDEETARKENPVDFALWKSAKPGEISWDSPWGPGRPGWHIECSVMSTEILGDTIDIHGGGA
DLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNFITVHDALKTLDGQVLRFFFATQHYRKPINFTEKA
VRDAETNLKYLKNTYEQPFTGNVDAQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFG
IVFVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD

Sequences:

>Translated_457_residues
MDFGFPDYYFMIKIYDTMSRDLREFVPIEDGKVKMYVCGPTVYNYIHVGNARSTVAFDTIRRYFEYRGYEVAYISNFTDV
DDKIINRAKEEGITPQEVADKYIAAFREDVTALGVKPATRHPRVVEFMDDIIRFVEDLIEKGFAYESQGDVYFRVEKSHN
YAKLANKTLEDLELGASGRTDEETARKENPVDFALWKSAKPGEISWDSPWGPGRPGWHIECSVMSTEILGDTIDIHGGGA
DLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNFITVHDALKTLDGQVLRFFFATQHYRKPINFTEKA
VRDAETNLKYLKNTYEQPFTGNVDAQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFG
IVFVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD
>Mature_457_residues
MDFGFPDYYFMIKIYDTMSRDLREFVPIEDGKVKMYVCGPTVYNYIHVGNARSTVAFDTIRRYFEYRGYEVAYISNFTDV
DDKIINRAKEEGITPQEVADKYIAAFREDVTALGVKPATRHPRVVEFMDDIIRFVEDLIEKGFAYESQGDVYFRVEKSHN
YAKLANKTLEDLELGASGRTDEETARKENPVDFALWKSAKPGEISWDSPWGPGRPGWHIECSVMSTEILGDTIDIHGGGA
DLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNFITVHDALKTLDGQVLRFFFATQHYRKPINFTEKA
VRDAETNLKYLKNTYEQPFTGNVDAQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFG
IVFVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI13375695, Length=491, Percent_Identity=34.4195519348269, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI303304987, Length=339, Percent_Identity=35.9882005899705, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI62240992, Length=339, Percent_Identity=35.9882005899705, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI10835051, Length=339, Percent_Identity=35.9882005899705, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI21269877, Length=339, Percent_Identity=35.9882005899705, Blast_Score=207, Evalue=2e-53,
Organism=Escherichia coli, GI1786737, Length=464, Percent_Identity=42.8879310344828, Blast_Score=351, Evalue=6e-98,
Organism=Caenorhabditis elegans, GI17509795, Length=245, Percent_Identity=40, Blast_Score=178, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17509797, Length=245, Percent_Identity=40, Blast_Score=178, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6324082, Length=232, Percent_Identity=45.6896551724138, Blast_Score=201, Evalue=2e-52,
Organism=Drosophila melanogaster, GI19922218, Length=506, Percent_Identity=33.00395256917, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI19922364, Length=237, Percent_Identity=40.5063291139241, Blast_Score=191, Evalue=8e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e [H]

EC number: =6.1.1.16 [H]

Molecular weight: Translated: 52266; Mature: 52266

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFGFPDYYFMIKIYDTMSRDLREFVPIEDGKVKMYVCGPTVYNYIHVGNARSTVAFDTI
CCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCEEEEEECCHHEEEEEECCCCCHHHHHHH
RRYFEYRGYEVAYISNFTDVDDKIINRAKEEGITPQEVADKYIAAFREDVTALGVKPATR
HHHHHHCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
HPRVVEFMDDIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLEDLELGASGRT
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCC
DEETARKENPVDFALWKSAKPGEISWDSPWGPGRPGWHIECSVMSTEILGDTIDIHGGGA
CHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEEHHHCCCEEEECCCCC
DLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNFITVHDALKTLDGQV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCEEHHHHHCCEEHHHHHHHHHHHHH
LRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVDAQELQNFKDKFVAAMD
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHC
EDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIVFVEEVLDAEIEDLIQKRQ
CCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD
HHHCCCCCHHHHHHHHHHHHCCHHEEECCCCCCCCCC
>Mature Secondary Structure
MDFGFPDYYFMIKIYDTMSRDLREFVPIEDGKVKMYVCGPTVYNYIHVGNARSTVAFDTI
CCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCEEEEEECCHHEEEEEECCCCCHHHHHHH
RRYFEYRGYEVAYISNFTDVDDKIINRAKEEGITPQEVADKYIAAFREDVTALGVKPATR
HHHHHHCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
HPRVVEFMDDIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLEDLELGASGRT
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCC
DEETARKENPVDFALWKSAKPGEISWDSPWGPGRPGWHIECSVMSTEILGDTIDIHGGGA
CHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEEHHHCCCEEEECCCCC
DLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNFITVHDALKTLDGQV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCEEHHHHHCCEEHHHHHHHHHHHHH
LRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVDAQELQNFKDKFVAAMD
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHC
EDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIVFVEEVLDAEIEDLIQKRQ
CCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD
HHHCCCCCHHHHHHHHHHHHCCHHEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]