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The map label for this gene is pheT [H]

Identifier: 169834362

GI number: 169834362

Start: 623903

End: 626308

Strand: Direct

Name: pheT [H]

Synonym: SPH_0680

Alternate gene names: 169834362

Gene position: 623903-626308 (Clockwise)

Preceding gene: 169832560

Following gene: 169833219

Centisome position: 27.78

GC content: 45.68

Gene sequence:

>2406_bases
ATGCTTGTATCTTATAAATGGTTAAAAGAATTGGTGGACATTGATGTGCCATCACAAGAGTTGGCTGAAAAAATGTCAAC
TACAGGAATCGAGGTAGAGGGTGTCGAATCACCAGCTGCTGGTCTCTCAAAAATTGTCGTCGGTGAGGTCTTGTCTTGCG
AAGATGTGCCAGAGACTCACCTCCATGTTTGTCAGGTTAACGTTGGCGAAGAAGAAGAGCGTCAGATCGTTTGTGGTGCC
CCAAATGTGCGTGCTGGGATCAAGGTCATGGTGGCTCTTCCAGGAGCTCGTATCGCTGATAACTACAAAATCAAAAAAGG
AAAAATCCGTGGTTTGGAGTCACTTGGAATGATCTGTTCACTTGGTGAATTGGGAATTTCTGACTCAGTTGTGCCTAAGG
AATTCGCAGATGGCATCCAAATCTTGCCTGAAGATGCCGTGCCAGGTGAGGAAGTCTTTTCTTACCTAGACTTGGATGAT
GAAATCATCGAACTTTCCATCACACCAAACCGTGCAGATGCCCTTTCTATGTGTGGAGTGGCTCACGAAGTGGCAGCCAT
CTATGACAAGGCAGTCAACTTTAAAAAATTTACTCTAACAGAAACTAATGAAGCTGCGGCAGATGCCCTTTCTGTCAGCA
TTGAGACAGACAAGGCGCCTTACTATGCAGCTCGTATCTTGGACAATGTGACTATCGCACCAAGTCCACAATGGTTGCAA
AACCTTCTCATGAACGAAGGCATCCGTCCCATCAATAACGTTGTAGACGTGACAAACTACATCCTGCTCTACTTTGGTCA
ACCTATGCATGCTTTTGACTTGGACACATTTGAAGGGACTGACATCCGTGTGCGTGAAGCGCGTGATGGTGAAAAATTAG
TGACCCTGGACGGTGAAGAACGAGACTTGGCTGAGACAGACCTCGTGATTACAGTTGCTGACAAACCAGTAGCCCTTGCC
GGTGTTATGGGTGGTCAGGCTACAGAAATTTCTGAAAAATCTAGTCGTGTTATCCTTGAAGCTGCTGTTTTTAATGGCAA
ATCTATCCGTAAGACAAGTGGTCGCCTGAACCTTCGTTCTGAGTCATCTTCTCGCTTTGAAAAAGGAATTAATGTGGCAA
CAGTTAATGAAGCCCTTGATGCGGCAGCTAGCATGATTGCAGAGCTTGCAGGTGCGACGGTGCGTAAGGGTATCGTTTCA
GCGGGTGAGCTTGATACTTCAGATGTAGAAGTTTCTTCAACTCTTGCTGATGTTAACCGTGTCCTCGGAACTGAGCTGTC
TTATGCTGATGTCGAAGACGTCTTCCGTCGTCTTGGCTTTGGTCTTTCTGGAAATGCAGACAGCTTTACAGTCAGCGTAC
CACGTCGTCGTTGGGATATCACAATCGAAGCTGATCTCTTTGAAGAAATCGCTCGTATCTATGGATATGACCGCTTGCCA
ACCAGCCTTCCAAAAGACGATGGTACAGCTGGTGAATTGACTGTGACACAAAAACTCCGCCGTCAAGTTCGTACCATTGC
TGAAGGAGCAGGTTTGACAGAAATCATCACCTATGCTCTGACAACTCCTGAAAAAGCAGTTGAGTTCACAGCTCAACCAA
GTAACCTTACGGAACTCATGTGGCCAATGACTGTGGATCGTTCAGTCCTCCGTCAAAATATGATTTCAGGGATCCTTGAT
ACCGTTGCCTACAACGTGGCTCGTAAGAATAAAAACTTGGCCCTTTACGAGATTGGAAAAGTCTTTGAACAAACAGGTAA
TCCAAAAGAAGAACTTCCAAATGAAATCAACAGTTTTGCCTTTGCCTTGACAGGCTTGGTTGCTGAAAAAGATTTCCAAA
CAGTAGCAGTTCCAGTTGATTTCTTCTATGCTAAAGGAATCCTTGAAGCCCTATTTACTCGCTTGGGACTAGAAGTGACC
TATACAGCAACAGCTGAGATTGCCAGCCTTCACCCAGGACGTACAGCAGTCATTTCACTCGGTGACCAAGTTCTTGGTTT
TCTTGGCCAAGTGCATCCAGTCACTGCTAAGGCCTACGATATTCCAGAAACGTATGTGGCTGAGCTCAACCTTTCAGTCA
TCGAAGCTGCCCTTCAACCTGCTGCTCCATTTGTAGAAATCACCAAATTCCCAGCAGTCAGCCGTGACGTTGCCCTTCTC
CTCAAGGCAGAAGTAACTCACCAAGAAGTTGTAGATGCTATCCAAGCTGCCGGCGTGAAACGTTTGACAGATATCAAACT
CTTTGACGTCTTCTCAGGTGAGAAATTGGGACTTGGTATGAAGTCCATGGCTTATAGTTTGACCTTCCAAAATCCAGAAG
ATAGCTTAACGGACGAAGAAGTCGCACGCTATATGGAAAAAATCCAAGCATCGCTCGAAGAAAAAGTGAATGCAGAAGTG
CGTTAA

Upstream 100 bases:

>100_bases
TTACTGAGAAAGAAATTAAGGTAAGAAGAGGATTCTTCTATTGAAATGATAGGAAAGGAAAAGAACTAGAGTGACAACTG
TCATTCTGGAGAACTAAATT

Downstream 100 bases:

>100_bases
ACGATAGTAGGATACAAATTCCCCCTCTAGTTTATGAACACTAGAGGGGTGATTTTATATAAAATAATTAAAATGTGATT
TCTGTAAAAATACATCTATG

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 801; Mature: 801

Protein sequence:

>801_residues
MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSKIVVGEVLSCEDVPETHLHVCQVNVGEEEERQIVCGA
PNVRAGIKVMVALPGARIADNYKIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDD
EIIELSITPNRADALSMCGVAHEVAAIYDKAVNFKKFTLTETNEAAADALSVSIETDKAPYYAARILDNVTIAPSPQWLQ
NLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDTFEGTDIRVREARDGEKLVTLDGEERDLAETDLVITVADKPVALA
GVMGGQATEISEKSSRVILEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASMIAELAGATVRKGIVS
AGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFTVSVPRRRWDITIEADLFEEIARIYGYDRLP
TSLPKDDGTAGELTVTQKLRRQVRTIAEGAGLTEIITYALTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILD
TVAYNVARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTVAVPVDFFYAKGILEALFTRLGLEVT
YTATAEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIPETYVAELNLSVIEAALQPAAPFVEITKFPAVSRDVALL
LKAEVTHQEVVDAIQAAGVKRLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNAEV
R

Sequences:

>Translated_801_residues
MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSKIVVGEVLSCEDVPETHLHVCQVNVGEEEERQIVCGA
PNVRAGIKVMVALPGARIADNYKIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDD
EIIELSITPNRADALSMCGVAHEVAAIYDKAVNFKKFTLTETNEAAADALSVSIETDKAPYYAARILDNVTIAPSPQWLQ
NLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDTFEGTDIRVREARDGEKLVTLDGEERDLAETDLVITVADKPVALA
GVMGGQATEISEKSSRVILEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASMIAELAGATVRKGIVS
AGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFTVSVPRRRWDITIEADLFEEIARIYGYDRLP
TSLPKDDGTAGELTVTQKLRRQVRTIAEGAGLTEIITYALTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILD
TVAYNVARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTVAVPVDFFYAKGILEALFTRLGLEVT
YTATAEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIPETYVAELNLSVIEAALQPAAPFVEITKFPAVSRDVALL
LKAEVTHQEVVDAIQAAGVKRLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNAEV
R
>Mature_801_residues
MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSKIVVGEVLSCEDVPETHLHVCQVNVGEEEERQIVCGA
PNVRAGIKVMVALPGARIADNYKIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDD
EIIELSITPNRADALSMCGVAHEVAAIYDKAVNFKKFTLTETNEAAADALSVSIETDKAPYYAARILDNVTIAPSPQWLQ
NLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDTFEGTDIRVREARDGEKLVTLDGEERDLAETDLVITVADKPVALA
GVMGGQATEISEKSSRVILEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASMIAELAGATVRKGIVS
AGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFTVSVPRRRWDITIEADLFEEIARIYGYDRLP
TSLPKDDGTAGELTVTQKLRRQVRTIAEGAGLTEIITYALTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILD
TVAYNVARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTVAVPVDFFYAKGILEALFTRLGLEVT
YTATAEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIPETYVAELNLSVIEAALQPAAPFVEITKFPAVSRDVALL
LKAEVTHQEVVDAIQAAGVKRLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNAEV
R

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=816, Percent_Identity=35.5392156862745, Blast_Score=424, Evalue=1e-119,
Organism=Drosophila melanogaster, GI21355211, Length=297, Percent_Identity=23.9057239057239, Blast_Score=73, Evalue=1e-12,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 87131; Mature: 87131

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSKIVVGEVLSCEDVPETH
CCCCHHHHHHHHCCCCCHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCC
LHVCQVNVGEEEERQIVCGAPNVRAGIKVMVALPGARIADNYKIKKGKIRGLESLGMICS
EEEEEEECCCCCCCEEEECCCCCCCCEEEEEECCCCEECCCCEEECCCCCCHHHHHHHHH
LGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDDEIIELSITPNRADALSMCGV
HCCCCCCCCCCCHHHHCCCEECCCCCCCHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHH
AHEVAAIYDKAVNFKKFTLTETNEAAADALSVSIETDKAPYYAARILDNVTIAPSPQWLQ
HHHHHHHHHHHCCCEEEEEECCCHHHHHEEEEEEECCCCCHHHHHHHCCCCCCCCHHHHH
NLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDTFEGTDIRVREARDGEKLVTLDGEE
HHHHHCCCCHHHHHHHHHHHEEEEECCCCCEECCCCCCCCCEEEEECCCCCEEEEECCCC
RDLAETDLVITVADKPVALAGVMGGQATEISEKSSRVILEAAVFNGKSIRKTSGRLNLRS
CCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCEEEEEEEEECCCCEEECCCCEEECC
ESSSRFEKGINVATVNEALDAAASMIAELAGATVRKGIVSAGELDTSDVEVSSTLADVNR
CCHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
VLGTELSYADVEDVFRRLGFGLSGNADSFTVSVPRRRWDITIEADLFEEIARIYGYDRLP
HHCCCCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEEEHHHHHHHHHHHCCCCCC
TSLPKDDGTAGELTVTQKLRRQVRTIAEGAGLTEIITYALTTPEKAVEFTAQPSNLTELM
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHEEECCCCCHHHHH
WPMTVDRSVLRQNMISGILDTVAYNVARKNKNLALYEIGKVFEQTGNPKEELPNEINSFA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCHHHHHHHHHHHH
FALTGLVAEKDFQTVAVPVDFFYAKGILEALFTRLGLEVTYTATAEIASLHPGRTAVISL
HHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHCCCCCEEEEEC
GDQVLGFLGQVHPVTAKAYDIPETYVAELNLSVIEAALQPAAPFVEITKFPAVSRDVALL
CHHHHHHHCCCCCCEEEECCCCHHHHHHCCHHHHHHHHCCCCCCEEECCCCCCCCCHHHH
LKAEVTHQEVVDAIQAAGVKRLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEE
HHHHCHHHHHHHHHHHCCCHHHHCEEEEEECCCCCCCCCHHHHEEEEEECCCCCCCCHHH
VARYMEKIQASLEEKVNAEVR
HHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSKIVVGEVLSCEDVPETH
CCCCHHHHHHHHCCCCCHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCC
LHVCQVNVGEEEERQIVCGAPNVRAGIKVMVALPGARIADNYKIKKGKIRGLESLGMICS
EEEEEEECCCCCCCEEEECCCCCCCCEEEEEECCCCEECCCCEEECCCCCCHHHHHHHHH
LGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDDEIIELSITPNRADALSMCGV
HCCCCCCCCCCCHHHHCCCEECCCCCCCHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHH
AHEVAAIYDKAVNFKKFTLTETNEAAADALSVSIETDKAPYYAARILDNVTIAPSPQWLQ
HHHHHHHHHHHCCCEEEEEECCCHHHHHEEEEEEECCCCCHHHHHHHCCCCCCCCHHHHH
NLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDTFEGTDIRVREARDGEKLVTLDGEE
HHHHHCCCCHHHHHHHHHHHEEEEECCCCCEECCCCCCCCCEEEEECCCCCEEEEECCCC
RDLAETDLVITVADKPVALAGVMGGQATEISEKSSRVILEAAVFNGKSIRKTSGRLNLRS
CCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCEEEEEEEEECCCCEEECCCCEEECC
ESSSRFEKGINVATVNEALDAAASMIAELAGATVRKGIVSAGELDTSDVEVSSTLADVNR
CCHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
VLGTELSYADVEDVFRRLGFGLSGNADSFTVSVPRRRWDITIEADLFEEIARIYGYDRLP
HHCCCCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEEEHHHHHHHHHHHCCCCCC
TSLPKDDGTAGELTVTQKLRRQVRTIAEGAGLTEIITYALTTPEKAVEFTAQPSNLTELM
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHEEECCCCCHHHHH
WPMTVDRSVLRQNMISGILDTVAYNVARKNKNLALYEIGKVFEQTGNPKEELPNEINSFA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCHHHHHHHHHHHH
FALTGLVAEKDFQTVAVPVDFFYAKGILEALFTRLGLEVTYTATAEIASLHPGRTAVISL
HHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHCCCCCEEEEEC
GDQVLGFLGQVHPVTAKAYDIPETYVAELNLSVIEAALQPAAPFVEITKFPAVSRDVALL
CHHHHHHHCCCCCCEEEECCCCHHHHHHCCHHHHHHHHCCCCCCEEECCCCCCCCCHHHH
LKAEVTHQEVVDAIQAAGVKRLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEE
HHHHCHHHHHHHHHHHCCCHHHHCEEEEEECCCCCCCCCHHHHEEEEEECCCCCCCCHHH
VARYMEKIQASLEEKVNAEVR
HHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]