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The map label for this gene is hsdS [H]

Identifier: 169834252

GI number: 169834252

Start: 917193

End: 918215

Strand: Direct

Name: hsdS [H]

Synonym: SPH_0997

Alternate gene names: 169834252

Gene position: 917193-918215 (Clockwise)

Preceding gene: 169832442

Following gene: 169833652

Centisome position: 40.84

GC content: 31.96

Gene sequence:

>1023_bases
ATGAAGAAAGTGAAATTAGGGCAAGTGGCTACTTTTATCAATGGCTATGCTTTTAAACCTCAAGATTGGTCATCTGAAGG
GAAAGAGATTATCAGAATACAGAATCTGACTAAAACTTCAAAAGGGATAAATTATTATTCGGGTACGATTGATAAAAAAT
ACATTGTTGAAGCTGGTGATATTCTTATTTCGTGGTCGGGTACATTAGGGGTTTTTCAGTGGTGTGGAAGATCCGCAGTG
TTAAATCAGCACATTTTTAAAGTGGTTTTTGATAAGATAGATATAGATAAATCGTATTTTAAATATGTTGTTGAAAAAGG
TTTACAAGATGCTGTAAAACATACTCATGGTTCCACAATGAAACATTTAACTAAAAAATATTTTGATAATATTATGGTTT
CATATACAAATTTGGGAGAGCAACAAAGAATTGCAAGTGAGCTGGATTTGTTAAGCAAGCTGATTTTAAGGAGACAAGAA
CAACTTGAAGAGCTTAATCTACTTGTAAAATCCCGATTTAACGAGATGTTTGGGGATCCTTTAAATAATAACAAGAAATT
TGCAGTAAAAACGGGACAACAGTGTTTTAAATTTTCAAGTGGAAAATTTCTTGATAAACATGATAGAGTTTTTGAAGGTT
ATCCTGCCTATGGTGGAAATGGCATTGCTTGGAAAAGTCGAAAATATTTAATTGATAATCCTACTATCATCATTGGTCGT
GTAGGTGCATATTGTGGTAATGTAAGAACTACCCACGGTAAGGTATGGATAAGTGACAATGCTATATATATAAAAGAATT
TAAGAATTCCGATTTCAATCTTGTATTTTTATTAGAATTAATGAAAGTAATTGATTTTAGTAAATTTGCTGATTTCTCAG
GTCAACCAAAGATAACTCAAAAACCATTAGAAAATCAGAAATATATCCTCCCCCCTCTAGCCCTCCAAAATGAGTTCGCA
GACTTTGTAGTTCAGATCGACAAATCACAATCAGAAATTTATTATCCATGTTTTAAGGAATAA

Upstream 100 bases:

>100_bases
AACCCTGGCCTATGCCATGGTCAATCAAAATAATGTCAAGCATTCACACCAAAAATTCATCTCTTAAAAGCAAATCCCGA
TATTCCGAGGAGGTGGTTGC

Downstream 100 bases:

>100_bases
TAATTATAGTCGAGTGATATCAGGAAGTGCTCAGCCTCAGTTACCAATTACAAAATTAAAAAAAATACTTCTCCCCCTCC
CCCCACTAGCCCTCCAAAAT

Product: restriction modification system DNA specificity subunit

Products: NA

Alternate protein names: S.EcoBI; Type I restriction enzyme EcoBI specificity protein; S protein [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MKKVKLGQVATFINGYAFKPQDWSSEGKEIIRIQNLTKTSKGINYYSGTIDKKYIVEAGDILISWSGTLGVFQWCGRSAV
LNQHIFKVVFDKIDIDKSYFKYVVEKGLQDAVKHTHGSTMKHLTKKYFDNIMVSYTNLGEQQRIASELDLLSKLILRRQE
QLEELNLLVKSRFNEMFGDPLNNNKKFAVKTGQQCFKFSSGKFLDKHDRVFEGYPAYGGNGIAWKSRKYLIDNPTIIIGR
VGAYCGNVRTTHGKVWISDNAIYIKEFKNSDFNLVFLLELMKVIDFSKFADFSGQPKITQKPLENQKYILPPLALQNEFA
DFVVQIDKSQSEIYYPCFKE

Sequences:

>Translated_340_residues
MKKVKLGQVATFINGYAFKPQDWSSEGKEIIRIQNLTKTSKGINYYSGTIDKKYIVEAGDILISWSGTLGVFQWCGRSAV
LNQHIFKVVFDKIDIDKSYFKYVVEKGLQDAVKHTHGSTMKHLTKKYFDNIMVSYTNLGEQQRIASELDLLSKLILRRQE
QLEELNLLVKSRFNEMFGDPLNNNKKFAVKTGQQCFKFSSGKFLDKHDRVFEGYPAYGGNGIAWKSRKYLIDNPTIIIGR
VGAYCGNVRTTHGKVWISDNAIYIKEFKNSDFNLVFLLELMKVIDFSKFADFSGQPKITQKPLENQKYILPPLALQNEFA
DFVVQIDKSQSEIYYPCFKE
>Mature_340_residues
MKKVKLGQVATFINGYAFKPQDWSSEGKEIIRIQNLTKTSKGINYYSGTIDKKYIVEAGDILISWSGTLGVFQWCGRSAV
LNQHIFKVVFDKIDIDKSYFKYVVEKGLQDAVKHTHGSTMKHLTKKYFDNIMVSYTNLGEQQRIASELDLLSKLILRRQE
QLEELNLLVKSRFNEMFGDPLNNNKKFAVKTGQQCFKFSSGKFLDKHDRVFEGYPAYGGNGIAWKSRKYLIDNPTIIIGR
VGAYCGNVRTTHGKVWISDNAIYIKEFKNSDFNLVFLLELMKVIDFSKFADFSGQPKITQKPLENQKYILPPLALQNEFA
DFVVQIDKSQSEIYYPCFKE

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 39113; Mature: 39113

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVKLGQVATFINGYAFKPQDWSSEGKEIIRIQNLTKTSKGINYYSGTIDKKYIVEAGD
CCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHHCCCCEECCCCCCEEEEEECC
ILISWSGTLGVFQWCGRSAVLNQHIFKVVFDKIDIDKSYFKYVVEKGLQDAVKHTHGSTM
EEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHH
KHLTKKYFDNIMVSYTNLGEQQRIASELDLLSKLILRRQEQLEELNLLVKSRFNEMFGDP
HHHHHHHHHHHEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LNNNKKFAVKTGQQCFKFSSGKFLDKHDRVFEGYPAYGGNGIAWKSRKYLIDNPTIIIGR
CCCCCEEEEECCHHHHHCCCCCCCCCCCCHHCCCCCCCCCCEEECCCEEEECCCEEEEEC
VGAYCGNVRTTHGKVWISDNAIYIKEFKNSDFNLVFLLELMKVIDFSKFADFSGQPKITQ
HHHHHCCCEEECCEEEEECCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
KPLENQKYILPPLALQNEFADFVVQIDKSQSEIYYPCFKE
CCCCCCCEECCCHHHHHHHHHHHEEECCCCCCEECCCCCC
>Mature Secondary Structure
MKKVKLGQVATFINGYAFKPQDWSSEGKEIIRIQNLTKTSKGINYYSGTIDKKYIVEAGD
CCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHHCCCCEECCCCCCEEEEEECC
ILISWSGTLGVFQWCGRSAVLNQHIFKVVFDKIDIDKSYFKYVVEKGLQDAVKHTHGSTM
EEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHH
KHLTKKYFDNIMVSYTNLGEQQRIASELDLLSKLILRRQEQLEELNLLVKSRFNEMFGDP
HHHHHHHHHHHEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LNNNKKFAVKTGQQCFKFSSGKFLDKHDRVFEGYPAYGGNGIAWKSRKYLIDNPTIIIGR
CCCCCEEEEECCHHHHHCCCCCCCCCCCCHHCCCCCCCCCCEEECCCEEEECCCEEEEEC
VGAYCGNVRTTHGKVWISDNAIYIKEFKNSDFNLVFLLELMKVIDFSKFADFSGQPKITQ
HHHHHCCCEEECCEEEEECCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
KPLENQKYILPPLALQNEFADFVVQIDKSQSEIYYPCFKE
CCCCCCCEECCCHHHHHHHHHHHEEECCCCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6304321 [H]