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The map label for this gene is malX [H]

Identifier: 169834248

GI number: 169834248

Start: 2106011

End: 2107282

Strand: Direct

Name: malX [H]

Synonym: SPH_2297

Alternate gene names: 169834248

Gene position: 2106011-2107282 (Clockwise)

Preceding gene: 169833581

Following gene: 169833655

Centisome position: 93.78

GC content: 41.19

Gene sequence:

>1272_bases
ATGTCATCTAAATTCACGAAGAGCGCTGCTGTGCTTGGAACTGTTACACTTGCTAGCTTGCTTTTGGTAGCTTGCGGAAG
CAAAACTGCTGATAAGCCTGCTGATTCTGGTTCATCTGAAGCCAAAGAACTCACTGTATATGTAGACGAAGGATATAAGA
GCTATATTGAAGAGATTGCTAAAGCTTATGAAAAAGAAGCTGGCGTAAAAATTACTCTTAAAACTGGTGATGCTCTAGGA
GGTCTTGATAAACTTTCACTTGACAACCAATCTGGTAATGTCCCTGATATTATGATGGCTCCATACGACCGTGTAGGTAG
CCTTGGTTCTGACGGACAACTTTCAGAAGTGAAATTGAGCGATGGTGCTAAAACAGACGACACAACTAAATCTCTTGTAA
CTGCTGCTAATGGTAAAGTTTACGGTGCTCCTGCCGTGATCGAGTCACTTGTTATGTACTACAACAAAGACTTGGTAAAA
GATGCTCCAAAAACATTTACTGACTTGGAAAACCTTGCTAAAGATAGCAAATATGCCTTCGCTGGAGAAGATGGCAAAAC
TACTGCCTTCCTAGCTGACTGGACAAACTTCTACTATGCATACGGACTTCTTGCTGGTAACGGCGGATACGTATTCGGAC
AAAACGGTAAAGATCCTAAAGACATCGGTCTTGCCAACGACGGTTCTATCGCAGGTATCAACTACGCTAAATCTTGGTAT
GAAAAATGGCCTAAAGGTATGCAAGATACAGAAGGTGCTGGAAACTTGATCCAAACTCATTTCCAAGAAGGTAAAACAGC
TGCTATCATCGACGGACCTTGGAAAGCTCAAGCTTTCAAAGATGCTAAAGTAAACTACGGAGTTGCAACTATTCCAACTC
TTCCAAATGGAAAAGAATATGCTGCATTCGGTGGTGGTAAAGCCTGGGTCATCCCTCAAGCCGTTAAGAACCTTGAAGCC
GCACAAAAATTTGTAGACTTCCTTGTTTCAACTGAACAACAAAAAGCCTTATACGATAAGACTAATGAAATCCCAGCTAA
TACTGAAGCTCGTTCATACGCTGAAGGTAAAAACGATGAGTTGACAACAGCTGTTATCAAACAGTTCAAGAGCACTCAGC
CACTGCCAAACATCTCTCAAATGTCTGCAGTTTGGGATCCAGCGAAAAACATGCTCTTTGATGCTGTAAGTGGTCAAAAA
GATGCTAAAACAGCTGCTAACGATGCTGTAACATTGATTAAAGAAACAATCAAACAAAAATTTGGTGAATAA

Upstream 100 bases:

>100_bases
CTTGCAACCGTTTTCTATTTGTGCTATACTAAGCTCATAAAGGAAAACGTTTGCGTTTTTCTTGTCAATAAAACTTGTTA
TTCTTTAGGAGGAATACACT

Downstream 100 bases:

>100_bases
CAAATTTGTTCAAGGGGGGTGGAAATCAAATCCCCCTTTGAATTTATCAATAGAAACACAAATAATTTAGCTTTCTTATA
AAAAAGCGGTATCCTATGAA

Product: maltose/maltodextrin-binding protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MSSKFTKSAAVLGTVTLASLLLVACGSKTADKPADSGSSEAKELTVYVDEGYKSYIEEIAKAYEKEAGVKITLKTGDALG
GLDKLSLDNQSGNVPDIMMAPYDRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
DAPKTFTDLENLAKDSKYAFAGEDGKTTAFLADWTNFYYAYGLLAGNGGYVFGQNGKDPKDIGLANDGSIAGINYAKSWY
EKWPKGMQDTEGAGNLIQTHFQEGKTAAIIDGPWKAQAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEA
AQKFVDFLVSTEQQKALYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKSTQPLPNISQMSAVWDPAKNMLFDAVSGQK
DAKTAANDAVTLIKETIKQKFGE

Sequences:

>Translated_423_residues
MSSKFTKSAAVLGTVTLASLLLVACGSKTADKPADSGSSEAKELTVYVDEGYKSYIEEIAKAYEKEAGVKITLKTGDALG
GLDKLSLDNQSGNVPDIMMAPYDRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
DAPKTFTDLENLAKDSKYAFAGEDGKTTAFLADWTNFYYAYGLLAGNGGYVFGQNGKDPKDIGLANDGSIAGINYAKSWY
EKWPKGMQDTEGAGNLIQTHFQEGKTAAIIDGPWKAQAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEA
AQKFVDFLVSTEQQKALYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKSTQPLPNISQMSAVWDPAKNMLFDAVSGQK
DAKTAANDAVTLIKETIKQKFGE
>Mature_422_residues
SSKFTKSAAVLGTVTLASLLLVACGSKTADKPADSGSSEAKELTVYVDEGYKSYIEEIAKAYEKEAGVKITLKTGDALGG
LDKLSLDNQSGNVPDIMMAPYDRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVKD
APKTFTDLENLAKDSKYAFAGEDGKTTAFLADWTNFYYAYGLLAGNGGYVFGQNGKDPKDIGLANDGSIAGINYAKSWYE
KWPKGMQDTEGAGNLIQTHFQEGKTAAIIDGPWKAQAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEAA
QKFVDFLVSTEQQKALYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKSTQPLPNISQMSAVWDPAKNMLFDAVSGQKD
AKTAANDAVTLIKETIKQKFGE

Specific function: Binding protein for maltose and maltodextrin; involved in its cellular uptake [H]

COG id: COG2182

COG function: function code G; Maltose-binding periplasmic proteins/domains

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790466, Length=381, Percent_Identity=30.9711286089239, Blast_Score=143, Evalue=2e-35,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006060
- InterPro:   IPR006059
- InterPro:   IPR006061 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 45287; Mature: 45156

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01037 SBP_BACTERIAL_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKFTKSAAVLGTVTLASLLLVACGSKTADKPADSGSSEAKELTVYVDEGYKSYIEEIA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHH
KAYEKEAGVKITLKTGDALGGLDKLSLDNQSGNVPDIMMAPYDRVGSLGSDGQLSEVKLS
HHHHHHCCEEEEEECCCCCCCCCCEECCCCCCCCCCEEECCCHHHCCCCCCCCCEEEEEC
DGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVKDAPKTFTDLENLAKDSKYAF
CCCCCCCHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEE
AGEDGKTTAFLADWTNFYYAYGLLAGNGGYVFGQNGKDPKDIGLANDGSIAGINYAKSWY
ECCCCCEEEEEEHHHHHHHHEEEEECCCCEEECCCCCCHHHCCCCCCCCEECCHHHHHHH
EKWPKGMQDTEGAGNLIQTHFQEGKTAAIIDGPWKAQAFKDAKVNYGVATIPTLPNGKEY
HHCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCHHHCCCCCCCCEEECCCCCCCCCE
AAFGGGKAWVIPQAVKNLEAAQKFVDFLVSTEQQKALYDKTNEIPANTEARSYAEGKNDE
EEECCCCEEECHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHCCCCCCH
LTTAVIKQFKSTQPLPNISQMSAVWDPAKNMLFDAVSGQKDAKTAANDAVTLIKETIKQK
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
FGE
HCC
>Mature Secondary Structure 
SSKFTKSAAVLGTVTLASLLLVACGSKTADKPADSGSSEAKELTVYVDEGYKSYIEEIA
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHH
KAYEKEAGVKITLKTGDALGGLDKLSLDNQSGNVPDIMMAPYDRVGSLGSDGQLSEVKLS
HHHHHHCCEEEEEECCCCCCCCCCEECCCCCCCCCCEEECCCHHHCCCCCCCCCEEEEEC
DGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVKDAPKTFTDLENLAKDSKYAF
CCCCCCCHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEE
AGEDGKTTAFLADWTNFYYAYGLLAGNGGYVFGQNGKDPKDIGLANDGSIAGINYAKSWY
ECCCCCEEEEEEHHHHHHHHEEEEECCCCEEECCCCCCHHHCCCCCCCCEECCHHHHHHH
EKWPKGMQDTEGAGNLIQTHFQEGKTAAIIDGPWKAQAFKDAKVNYGVATIPTLPNGKEY
HHCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCHHHCCCCCCCCEEECCCCCCCCCE
AAFGGGKAWVIPQAVKNLEAAQKFVDFLVSTEQQKALYDKTNEIPANTEARSYAEGKNDE
EEECCCCEEECHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHCCCCCCH
LTTAVIKQFKSTQPLPNISQMSAVWDPAKNMLFDAVSGQKDAKTAANDAVTLIKETIKQK
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
FGE
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11463916; 2684766 [H]