Click here to switch to the map view.

The map label for this gene is glyA [H]

Identifier: 169834123

GI number: 169834123

Start: 1036628

End: 1037884

Strand: Direct

Name: glyA [H]

Synonym: SPH_1127

Alternate gene names: 169834123

Gene position: 1036628-1037884 (Clockwise)

Preceding gene: 169833340

Following gene: 169833494

Centisome position: 46.16

GC content: 42.0

Gene sequence:

>1257_bases
ATGATTTTTGACAAAGATGATTTTAAAGCATATGATGCTGATCTCTGGAATGCTATTGCCAAAGAAGAAGAACGCCAACA
AAATAATATCGAGTTAATTGCTTCGGAAAACGTAGTTTCCAAGGCTGTTATGGCAGCTCAAGGGTCTATCTTGACAAATA
AATATGCCGAGGGTTACCCAGGACGCCGTTATTATGGTGGAACTGATGTAGTAGACGTTGTAGAGACTCTTGCTATTGAA
CGCGCAAAAGAAATTTTCGGTGCTAAATTTGCCAATGTCCAACCACATTCAGGAAGTCAGGCCAACTGTGCGGCTTACAT
ATCCTTGATTGAGCCAGGTGATACGGTTATGGGAATGGATTTGGCATCAGGTGGTCATTTGACTCATGGGGCTCCTGTTA
GCTTCTCTGGTCAAACCTACAACTTTGTTTCTTATAGTGTTGATCCTGAAACGGAACTCTTAGACTTTGATGCTATCTTG
AAACAAGCCCAAGAAGTAAAACCAAAACTGATTGTAGCTGGTGCTTCAGCATATTCTCAAATTATCGATTTTTCAAAATT
CCGTGAAATCGCAGATGCTGTCGGTGCGAAGCTCATGGTGGACATGGCCCATATCGCTGGTTTGGTTGCGGCTGGCCTTC
ATCCAAGCCCAGTTCCATACGCTCATATCACAACAACAACGACCCACAAAACCCTTCGTGGACCTCGTGGTGGTTTGATT
TTGACCAATGACGAAGAACTTGCTAAAAAAATTAATTCAGCTATTTTCCCAGGTATTCAGGGCGGTCCTTTAGAGCATGT
TGTGGCGGCTAAGGCAGTTTCCTTCAAAGAAGTTTTGGATCCAGCCTTCAAGGAATATGCTGCCAATGTAATTAAGAACA
GCAAGGCTATGGCAGATGTCTTCTTGCAAGACCCTGATTTCCGTATTATTTCAGGTGGAACTGAAAACCATCTCTTCCTT
GTTGATGTGACTAAGGTTGTAGAAAACGGAAAAGTTGCTCAAAACTTGCTGGATGAAGTCAATATTACCCTAAATAAAAA
TTCAATCCCTTACGAAACCTTGTCACCATTCAAGACAAGTGGGATTCGTATCGGAGCAGCAGCTATTACTGCACGTGGAT
TTGGTGAAGAAGAAAGTCGCAAAGTGGCTGAACTCATCATTAAAACCCTTAAGAATTCAGAAAATGAGGCTGTATTAGAA
GAAGTGAGAAGTGCAGTCAAAGAATTGACAGATGCCTTCCCATTATACGAGGAATAA

Upstream 100 bases:

>100_bases
TGTGATAAAATGCAGAAACGGTTTATTCGCATCTTTTAGTTTGATTTTCTTATTGTAAAATCAAACTAATGGACTAGTCA
CACAATAAAGGAGAAGACCT

Downstream 100 bases:

>100_bases
AACTTTTATGGACATTTATATTAAGAAAGCCATTATTCACCAGTTCAGTCCGGATGATACCGAGCTGTTCTTAGCAGATA
AGTTTCTCAATATTACTCCA

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVMAAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIE
RAKEIFGAKFANVQPHSGSQANCAAYISLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPVPYAHITTTTTHKTLRGPRGGLI
LTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFKEVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFL
VDVTKVVENGKVAQNLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKNSENEAVLE
EVRSAVKELTDAFPLYEE

Sequences:

>Translated_418_residues
MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVMAAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIE
RAKEIFGAKFANVQPHSGSQANCAAYISLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPVPYAHITTTTTHKTLRGPRGGLI
LTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFKEVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFL
VDVTKVVENGKVAQNLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKNSENEAVLE
EVRSAVKELTDAFPLYEE
>Mature_418_residues
MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVMAAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIE
RAKEIFGAKFANVQPHSGSQANCAAYISLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPVPYAHITTTTTHKTLRGPRGGLI
LTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFKEVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFL
VDVTKVVENGKVAQNLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKNSENEAVLE
EVRSAVKELTDAFPLYEE

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI19923315, Length=448, Percent_Identity=42.4107142857143, Blast_Score=358, Evalue=4e-99,
Organism=Homo sapiens, GI261862352, Length=448, Percent_Identity=42.4107142857143, Blast_Score=358, Evalue=4e-99,
Organism=Homo sapiens, GI261862350, Length=448, Percent_Identity=42.4107142857143, Blast_Score=358, Evalue=4e-99,
Organism=Homo sapiens, GI261862348, Length=448, Percent_Identity=42.4107142857143, Blast_Score=358, Evalue=4e-99,
Organism=Homo sapiens, GI261862346, Length=443, Percent_Identity=42.2121896162528, Blast_Score=342, Evalue=4e-94,
Organism=Homo sapiens, GI22547186, Length=427, Percent_Identity=42.1545667447307, Blast_Score=318, Evalue=8e-87,
Organism=Homo sapiens, GI22547189, Length=413, Percent_Identity=39.9515738498789, Blast_Score=283, Evalue=3e-76,
Organism=Escherichia coli, GI1788902, Length=408, Percent_Identity=59.0686274509804, Blast_Score=478, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI25144732, Length=426, Percent_Identity=43.6619718309859, Blast_Score=347, Evalue=6e-96,
Organism=Caenorhabditis elegans, GI25144729, Length=426, Percent_Identity=43.6619718309859, Blast_Score=347, Evalue=1e-95,
Organism=Saccharomyces cerevisiae, GI6319739, Length=426, Percent_Identity=43.8967136150235, Blast_Score=352, Evalue=6e-98,
Organism=Saccharomyces cerevisiae, GI6323087, Length=438, Percent_Identity=41.0958904109589, Blast_Score=325, Evalue=1e-89,
Organism=Drosophila melanogaster, GI24640005, Length=449, Percent_Identity=42.5389755011136, Blast_Score=357, Evalue=9e-99,
Organism=Drosophila melanogaster, GI221329721, Length=449, Percent_Identity=42.5389755011136, Blast_Score=356, Evalue=1e-98,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 45255; Mature: 45255

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVMAAQGSILTNKYAEGYP
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCHHHHHHHCCCC
GRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQANCAAYISLIEPGDTVMGMD
CCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEE
LASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAILKQAQEVKPKLIVAGASAYSQ
ECCCCCCCCCCCCEECCCEEEEEEEECCCCHHHCCHHHHHHHHHHCCCEEEEECCHHHHH
IIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPVPYAHITTTTTHKTLRGPRGGLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHCCCCCCEE
LTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFKEVLDPAFKEYAANVIKNSKAMADV
EECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
FLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQNLLDEVNITLNKNSIPYETLSPFKTS
HCCCCCEEEEECCCCCCEEEEEHHHHHCCCHHHHHHHHHCEEEECCCCCCCHHCCCCCCC
GIRIGAAAITARGFGEEESRKVAELIIKTLKNSENEAVLEEVRSAVKELTDAFPLYEE
CEEEEHHHHEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVMAAQGSILTNKYAEGYP
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCHHHHHHHCCCC
GRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQANCAAYISLIEPGDTVMGMD
CCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEE
LASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAILKQAQEVKPKLIVAGASAYSQ
ECCCCCCCCCCCCEECCCEEEEEEEECCCCHHHCCHHHHHHHHHHCCCEEEEECCHHHHH
IIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPVPYAHITTTTTHKTLRGPRGGLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHCCCCCCEE
LTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFKEVLDPAFKEYAANVIKNSKAMADV
EECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
FLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQNLLDEVNITLNKNSIPYETLSPFKTS
HCCCCCEEEEECCCCCCEEEEEHHHHHCCCHHHHHHHHHCEEEECCCCCCCHHCCCCCCC
GIRIGAAAITARGFGEEESRKVAELIIKTLKNSENEAVLEEVRSAVKELTDAFPLYEE
CEEEEHHHHEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA