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The map label for this gene is nusA [H]

Identifier: 169834095

GI number: 169834095

Start: 597322

End: 598458

Strand: Direct

Name: nusA [H]

Synonym: SPH_0650

Alternate gene names: 169834095

Gene position: 597322-598458 (Clockwise)

Preceding gene: 169833553

Following gene: 169833344

Centisome position: 26.6

GC content: 43.18

Gene sequence:

>1137_bases
ATGAGTAAAGAAATGCTAGAGGCCTTCCGCATTTTGGAAGAAGACAAGGGAATCAAAAAAGAAGATATCATCGACGCAGT
AGTAGAGTCGCTTCGTTCCGCTTATCGCAGACGCTATGGTCAGTCAGACAGCGTAGCTATTGACTTCAACGAAAAAACAG
GTGACTTTACAGTTTATACTGTCCGTGAAGTTGTTGATGAAGTATTTGATAGCCGTTTGGAAATCAGCTTGAAAGATGCT
CTTGCCATTAATTCAGCTTATGAACTTGGAGACAAAATCAAGTTTGAAGAAGCACCAGCTGAGTTTGGTCGTGTAGCAGC
CCAATCTGCCAAACAAACCATCATGGAAAAAATGCGCAAGCAAACACGTGCCATCACTTACAATACTTACAAAGAACATG
AGCAAGAAATCATGTCTGGTACAGTAGAACGCTTTGACAACCGCTTTATCTATGTCAACCTTGGTAGCATCGAAGCCCAA
TTGTCAAAACAAGACCAAATTCCTGGAGAAGTTTTTGCTTCTCATGATCGTATCGAAGTTTATGTTTACAAGGTTGAAGA
CAACCCTCGTGGTGTGAACGTCTTTGTTAGCCGTAGCCATCCAGAAATGATCAAACGTTTGATGGAGCAAGAAATTCCAG
AAGTTTATGATGGAACTGTTGAAATCATGAGCGTGGCTCGTGAAGCAGGTGACCGTACGAAGGTTGCTGTTCGTAGCCAC
AATCCAAACGTGGATGCTATCGGTACAATCGTTGGACGTGGTGGTGCTAATATCAAGAAGATTACTAGCAAATTCCACCC
AGCTCGTTACGATGCTAAAAATGACCGCATGGTACCAATCGAAGAAAATATCGATGTTATCGAGTGGGTAGCAGATCCAG
CTGAATTTATCTACAATGCCATCGCTCCTGCTGAGGTTGACCAAGTTATCTTTGATGAAAACGACAGCAAACGTGCCTTG
GTGGTTGTTCCAGATAACAAGCTTTCTCTTGCCATTGGTCGTCGTGGACAAAACGTGCGCTTGGCGGCTCACTTGACTGG
TTACCGTATCGATATCAAGTCTGCTAGCGAATTTGAAGCCATGGAAGACGCTGCTTCAGTAGAGTTGGAAGTAGAAAACG
ATACTGTAGAAGAATAA

Upstream 100 bases:

>100_bases
ACCGTCCGAATTCCATACAGTTTAGTATCAAAAGCACGTTTAGCAGTTAAATTATAGAAAAAGAAAGGATAGCTTTTGAG
GATTCAAAAGTGAAGAAAAC

Downstream 100 bases:

>100_bases
AAGCTGCTAGAGGAGGGAAAGATGAAAACAAGAAAAATCCCTTTGCGCAAGTCTGTTGTGTCTAACGAAGTGATTGATAA
GCGTGATTTGCTCCGCATTG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDSVAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDA
LAINSAYELGDKIKFEEAPAEFGRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDGTVEIMSVAREAGDRTKVAVRSH
NPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDRMVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRAL
VVVPDNKLSLAIGRRGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE

Sequences:

>Translated_378_residues
MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDSVAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDA
LAINSAYELGDKIKFEEAPAEFGRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDGTVEIMSVAREAGDRTKVAVRSH
NPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDRMVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRAL
VVVPDNKLSLAIGRRGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE
>Mature_377_residues
SKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDSVAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDAL
AINSAYELGDKIKFEEAPAEFGRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQL
SKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDGTVEIMSVAREAGDRTKVAVRSHN
PNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDRMVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALV
VVPDNKLSLAIGRRGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=365, Percent_Identity=33.1506849315069, Blast_Score=222, Evalue=3e-59,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 42798; Mature: 42666

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS50084 KH_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDSVAIDFNEKTGDFTVYT
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEE
VREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEFGRVAAQSAKQTIMEKMRK
HHHHHHHHHCCCEEEEEHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHH
QTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQLSKQDQIPGEVFASHDRIEV
HHHEEEECHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHCCCCCHHHHCCCCEEEE
YVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDGTVEIMSVAREAGDRTKVAVRSH
EEEEECCCCCCEEEEEECCCHHHHHHHHHHHCHHHHCCHHHHHHHHHHCCCCCEEEEECC
NPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDRMVPIEENIDVIEWVADPAEFIYNA
CCCCCEEEEEEECCCCCHHHHHHHCCCCCCCCCCCCEEEECCCCCCEEHHCCHHHHHHHH
IAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGRRGQNVRLAAHLTGYRIDIKSASEFEA
CCHHHHHHHEECCCCCCEEEEEECCCCEEEEECCCCCCEEEEEEEEEEEEEECCCHHHHH
MEDAASVELEVENDTVEE
HHCCCEEEEEECCCCCCC
>Mature Secondary Structure 
SKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDSVAIDFNEKTGDFTVYT
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEE
VREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEFGRVAAQSAKQTIMEKMRK
HHHHHHHHHCCCEEEEEHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHH
QTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQLSKQDQIPGEVFASHDRIEV
HHHEEEECHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHCCCCCHHHHCCCCEEEE
YVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDGTVEIMSVAREAGDRTKVAVRSH
EEEEECCCCCCEEEEEECCCHHHHHHHHHHHCHHHHCCHHHHHHHHHHCCCCCEEEEECC
NPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDRMVPIEENIDVIEWVADPAEFIYNA
CCCCCEEEEEEECCCCCHHHHHHHCCCCCCCCCCCCEEEECCCCCCEEHHCCHHHHHHHH
IAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGRRGQNVRLAAHLTGYRIDIKSASEFEA
CCHHHHHHHEECCCCCCEEEEEECCCCEEEEECCCCCCEEEEEEEEEEEEEECCCHHHHH
MEDAASVELEVENDTVEE
HHCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8491709; 9384377 [H]