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The map label for this gene is gdh [H]

Identifier: 169834065

GI number: 169834065

Start: 1336233

End: 1337579

Strand: Direct

Name: gdh [H]

Synonym: SPH_1446

Alternate gene names: 169834065

Gene position: 1336233-1337579 (Clockwise)

Preceding gene: 169834464

Following gene: 169833730

Centisome position: 59.5

GC content: 42.61

Gene sequence:

>1347_bases
ATGACATCTGCTAAAGAATATATCCAAAGCGTGTTTGAAACTGTGAAAGCTCGTAACGGGCACGAGGCTGAATTCCTCCA
AGCTGTTGAAGAATTTTTCAACACTTTGGAACCTGTATTTGAAAAACACCCTGAGTATATCGAAGAAAATATCTTGGCAC
GTATTACTGAGCCTGAGCGCGTGATTTCTTTCCGTGTTCCTTGGGTTGACCGTAATGGAAAAGTTCAAGTAAACCGTGGT
TACCGTGTTCAATTCAACTCAGCTGTTGGACCATACAAAGGTGGACTTCGTTTCCACCCAACTGTAAACCAAGGGATCTT
GAAATTCCTCGGATTTGAACAAATCTTTAAAAATGTCTTGACTGGACTTCCTATCGGTGGAGGTAAAGGTGGATCAGACT
TCGATCCTAAAGGTAAAACAGATGCTGAAGTGATGCGCTTCTGCCAAAGCTTCATGACTGAATTGCAAAAACACATCGGA
CCATCACTTGACGTACCTGCTGGTGATATCGGTGTTGGTGGACGTGAAATTGGTTACCTTTACGGTCAATACAAACGTCT
TAACCAATTTGATGCTGGTGTCTTGACTGGTAAACCTCTTGGATTTGGTGGTAGCTTGATTCGTCCAGAAGCAACTGGTT
ACGGTTTGGTTTACTATACTGAAGAAATGCTCAAAGCTAACGGTAACAGCTTTGCTGGTAAGAAAGTCGTTATTTCAGGT
TCTGGTAACGTTGCTCAATATGCTCTTCAAAAAGCAACTGAACTCGGTGCAACTGTTATTTCTGTTTCTGACTCAAATGG
TTATGTCATCGATGAAAATGGTATTGACTTCGATCTTTTGGTTGATGTTAAAGAAAAACGTCGTGCTCGTTTGACTGAGT
ATGCAGCTGAAAAAGCAACTGCAACCTACCACGAAGGTACTGTATGGACTTACGCTGGAAACTATGACATTGCTCTTCCA
TGTGCCACTCAAAACGAAATCAACGGTGAAGCAGCTAAACGTTTGGTTGCTCAAGGCGTTATCTGTGTATCTGAAGGTGC
CAACATGCCGAGCGACCTTGATGCCATCAAAGTTTACAAAGAAAATGGTATCTTCTACGGACCTGCCAAAGCTGCCAACG
CTGGTGGTGTAGCCGTTTCAGCCCTTGAAATGAGCCAAAATAGTCTTCGCCTCTCATGGACTCGTGAAGAAGTTGATGGC
CGTCTCAAAGACATCATGACAAACATCTTCAACACAGCTAAAACGACTTCAGAAACATATGGTCTTGATAAAGACTACCT
TGCAGGAGCAAACATTGCTGCCTTTGAAAATGTAGCAAACGCTATGATTGCCCAAGGTATTGTTTAA

Upstream 100 bases:

>100_bases
TGAAAGAATTTTTAGAAAATTTCTGTTTTTTCTTTACAGTGAAAAAAAATTCTGCTATAATGTCTTATGTAATAAAATAT
GACAATAAAAGGAGAACGAT

Downstream 100 bases:

>100_bases
GATTTATTTGCTTAATCCTCAATCACTACGATTGGGGATTTTTAGTTGAGTTAGACAGTTGGGAAATTCTAATTAAATGA
AATAAAAACTGAACAAATTG

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHPEYIEENILARITEPERVISFRVPWVDRNGKVQVNRG
YRVQFNSAVGPYKGGLRFHPTVNQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVYYTEEMLKANGNSFAGKKVVISG
SGNVAQYALQKATELGATVISVSDSNGYVIDENGIDFDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALP
CATQNEINGEAAKRLVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLSWTREEVDG
RLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV

Sequences:

>Translated_448_residues
MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHPEYIEENILARITEPERVISFRVPWVDRNGKVQVNRG
YRVQFNSAVGPYKGGLRFHPTVNQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVYYTEEMLKANGNSFAGKKVVISG
SGNVAQYALQKATELGATVISVSDSNGYVIDENGIDFDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALP
CATQNEINGEAAKRLVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLSWTREEVDG
RLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV
>Mature_447_residues
TSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHPEYIEENILARITEPERVISFRVPWVDRNGKVQVNRGY
RVQFNSAVGPYKGGLRFHPTVNQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIGP
SLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVYYTEEMLKANGNSFAGKKVVISGS
GNVAQYALQKATELGATVISVSDSNGYVIDENGIDFDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPC
ATQNEINGEAAKRLVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLSWTREEVDGR
LKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI4885281, Length=398, Percent_Identity=29.1457286432161, Blast_Score=152, Evalue=9e-37,
Organism=Homo sapiens, GI31377775, Length=412, Percent_Identity=28.8834951456311, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI1788059, Length=444, Percent_Identity=55.1801801801802, Blast_Score=503, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17544676, Length=360, Percent_Identity=29.7222222222222, Blast_Score=129, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6319256, Length=455, Percent_Identity=53.6263736263736, Blast_Score=432, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6324951, Length=453, Percent_Identity=53.4216335540839, Blast_Score=430, Evalue=1e-121,
Organism=Drosophila melanogaster, GI24649283, Length=407, Percent_Identity=29.97542997543, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI45549226, Length=359, Percent_Identity=31.7548746518106, Blast_Score=145, Evalue=8e-35,
Organism=Drosophila melanogaster, GI45553475, Length=359, Percent_Identity=31.7548746518106, Blast_Score=144, Evalue=9e-35,
Organism=Drosophila melanogaster, GI24649460, Length=294, Percent_Identity=32.6530612244898, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 48811; Mature: 48679

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHPEYIEENILARITEPER
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCC
VISFRVPWVDRNGKVQVNRGYRVQFNSAVGPYKGGLRFHPTVNQGILKFLGFEQIFKNVL
EEEEECCEECCCCEEEECCCEEEEECCCCCCCCCCEEECCCHHHHHHHHHCHHHHHHHHH
TGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIGPSLDVPAGDIGVGGREIGYL
HCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
YGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVYYTEEMLKANGNSFAGKKVVISG
HHHHHHHHHCCCCEECCCCCCCCCCEECCCCCCCEEEEEEHHHHHCCCCCCCCCEEEEEC
SGNVAQYALQKATELGATVISVSDSNGYVIDENGIDFDLLVDVKEKRRARLTEYAAEKAT
CCCHHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCEEEEEECHHHHHHHHHHHHHHHHC
ATYHEGTVWTYAGNYDIALPCATQNEINGEAAKRLVAQGVICVSEGANMPSDLDAIKVYK
CEEECCEEEEEECCEEEEEECCCCCCCCHHHHHHHHHHCCEEEECCCCCCCCCHHHEEEE
ENGIFYGPAKAANAGGVAVSALEMSQNSLRLSWTREEVDGRLKDIMTNIFNTAKTTSETY
CCCEEECCCCCCCCCCEEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHC
GLDKDYLAGANIAAFENVANAMIAQGIV
CCCHHHHCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHPEYIEENILARITEPER
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCC
VISFRVPWVDRNGKVQVNRGYRVQFNSAVGPYKGGLRFHPTVNQGILKFLGFEQIFKNVL
EEEEECCEECCCCEEEECCCEEEEECCCCCCCCCCEEECCCHHHHHHHHHCHHHHHHHHH
TGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIGPSLDVPAGDIGVGGREIGYL
HCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
YGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVYYTEEMLKANGNSFAGKKVVISG
HHHHHHHHHCCCCEECCCCCCCCCCEECCCCCCCEEEEEEHHHHHCCCCCCCCCEEEEEC
SGNVAQYALQKATELGATVISVSDSNGYVIDENGIDFDLLVDVKEKRRARLTEYAAEKAT
CCCHHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCEEEEEECHHHHHHHHHHHHHHHHC
ATYHEGTVWTYAGNYDIALPCATQNEINGEAAKRLVAQGVICVSEGANMPSDLDAIKVYK
CEEECCEEEEEECCEEEEEECCCCCCCCHHHHHHHHHHCCEEEECCCCCCCCCHHHEEEE
ENGIFYGPAKAANAGGVAVSALEMSQNSLRLSWTREEVDGRLKDIMTNIFNTAKTTSETY
CCCEEECCCCCCCCCCEEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHC
GLDKDYLAGANIAAFENVANAMIAQGIV
CCCHHHHCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]