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The map label for this gene is dapE [H]

Identifier: 169834042

GI number: 169834042

Start: 229560

End: 230933

Strand: Direct

Name: dapE [H]

Synonym: SPH_0262

Alternate gene names: 169834042

Gene position: 229560-230933 (Clockwise)

Preceding gene: 169833558

Following gene: 169833932

Centisome position: 10.22

GC content: 46.14

Gene sequence:

>1374_bases
ATGGTTTTTCCTAGCGAACAAGAACAGATTGAAAAATTTGAAAAGGATCATGTAGCCCAGCATTATTTTGAGGTTTTGCG
TACCTTGATTTCTAAGAAATCAGTCTTTGCCCAGCAGGTTGGACTCAAGGAAGTCGCAAATTATCTGGGTGAGATTTTCA
AGCGTGTTGGAGCTGAAGTGGAGATTGATGAGAGCTATACAGCGCCCTTTGTCATGGCACATTTCAAGAGTTCGCGTCCA
GATGCCAAGACCTTGATTTTCTATAACCACTATGACACTGTGCCAGCGGATGGGGATCAGGTCTGGACAGAGGATCCTTT
TACGCTTTCGGTCCGCAATGGCTTCATGTATGGGCGTGGAGTTGATGACGACAAGGGTCATATCACAGCTCGCTTGAGTG
CTTTGAGAAAATATATGCAGCACCATGATGATTTACCTGTCAATATCAGCTTTATCATGGAGGGAGCGGAGGAATCGGCT
TCAACAGACCTAGATAAGTATTTGGAAAAGCATGCAGACAAACTCCGTGGGGCGGATTTGTTGGTCTGGGAACAAGGGAC
CAAAAATGCCTTGGAACAGCTGGAAATTTCTGGTGGCAATAAGGGGATTGTGACCTTTGATGCCAAGGTAAAAAGCGCTG
ATGTGGATATCCACTCGAGTTATGGTGGTGTTGTGGAATCAGCTCCTTGGTATCTCCTCCAAGCCTTACAGTCTCTTCGT
GCTGCGGATGGCCGTATCTTGGTTGAAGGCTTGTACGAAGAAGTACAAGAGCCCAATGAACGAGAAATGGCCTTGCTAGA
AACTTATGGTCAACGAAACCCAGAGGAAGTTAGTCGGATTTATGGATTGGAGTTGCCTCTCTTACAGGAGGAGCGGATGG
CCTTTCTAAAACGTTTCTTTTTCGAGCCAGCGCTTAATATCGAAGGAATCCAGTCTGGTTATCAAGGTCAGGGTGTTAAG
ACTATTTTACCTGCAGAAGCCAGTGCCAAGCTAGAGGTTCGTCTGGTTCCGGGCCTAGAACCGCATGATGTTCTGGAAAA
AATTCGGAAACAGCTAGACAAAAATGGCTTTGATAAGGTAGAATTATACTATACCTTGGGAGAGATGAGCTATCGAAGCG
ATATGAGCGCACCAGCCATTCTCAATGTGATCGAGTTGGCCAAGAAATTCTATCCACAGGGCGTTTCAGTCTTGCCGACG
ACAGCGGGGACAGGACCTATGCATACGGTCTTTGATGCCCTAGAGGTACCAATGGTTGCATTCGGTCTAGGAAATGCCAA
TAGCCGAGACCACGGTGGAGATGAAAATGTGCGAATCGCTGATTATTACACCCATATCGAATTAGTAGAGGAGCTGATTA
GAAGCTATGAGTAG

Upstream 100 bases:

>100_bases
ATAAGAAACAGGGAGGTGGGAGAGAGAATTCCACCTCTTGCTTTTGTATAGAGCACGGAGTGTAAAGAAGACTATTCGTT
CATAGAAAGGTAGAGAGAAT

Downstream 100 bases:

>100_bases
AGATATTATCAAGTTAGATCAGATCGATGTGACTTTTCACCAAAAGAAGAGAACCATTACAGCGGTTAAGGATGTGACCA
TTCACATCCAAGAAGGGGAT

Product: acetylornithine deacetylase/succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKEVANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRP
DAKTLIFYNHYDTVPADGDQVWTEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIHSSYGGVVESAPWYLLQALQSLR
AADGRILVEGLYEEVQEPNEREMALLETYGQRNPEEVSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVK
TILPAEASAKLEVRLVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYPQGVSVLPT
TAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVEELIRSYE

Sequences:

>Translated_457_residues
MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKEVANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRP
DAKTLIFYNHYDTVPADGDQVWTEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIHSSYGGVVESAPWYLLQALQSLR
AADGRILVEGLYEEVQEPNEREMALLETYGQRNPEEVSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVK
TILPAEASAKLEVRLVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYPQGVSVLPT
TAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVEELIRSYE
>Mature_457_residues
MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKEVANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRP
DAKTLIFYNHYDTVPADGDQVWTEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIHSSYGGVVESAPWYLLQALQSLR
AADGRILVEGLYEEVQEPNEREMALLETYGQRNPEEVSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVK
TILPAEASAKLEVRLVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYPQGVSVLPT
TAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVEELIRSYE

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI21071039, Length=313, Percent_Identity=33.2268370607029, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI271398239, Length=388, Percent_Identity=29.3814432989691, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI271398277, Length=313, Percent_Identity=27.4760383386581, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17563146, Length=335, Percent_Identity=29.5522388059701, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI193211059, Length=382, Percent_Identity=25.3926701570681, Blast_Score=129, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6321159, Length=399, Percent_Identity=26.0651629072682, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6319758, Length=455, Percent_Identity=23.5164835164835, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24585880, Length=368, Percent_Identity=27.1739130434783, Blast_Score=132, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 51487; Mature: 51487

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKEVANYLGEIFKRVGAEV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCE
EIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVWTEDPFTLSVRNGFMYGRG
EECCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCEEECCC
VDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESASTDLDKYLEKHADKLRGADL
CCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHCCHHHHHHHHHHHHHCCCEE
LVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIHSSYGGVVESAPWYLLQALQSLR
EEECCCHHHHHHHHEECCCCCEEEEEECEEEECCCEEECCCCCCHHCCHHHHHHHHHHHH
AADGRILVEGLYEEVQEPNEREMALLETYGQRNPEEVSRIYGLELPLLQEERMAFLKRFF
HCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHH
FEPALNIEGIQSGYQGQGVKTILPAEASAKLEVRLVPGLEPHDVLEKIRKQLDKNGFDKV
HCCCCCHHHHCCCCCCCCCEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCEE
ELYYTLGEMSYRSDMSAPAILNVIELAKKFYPQGVSVLPTTAGTGPMHTVFDALEVPMVA
EEEEEECCHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHCCHHE
FGLGNANSRDHGGDENVRIADYYTHIELVEELIRSYE
EECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKEVANYLGEIFKRVGAEV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCE
EIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVWTEDPFTLSVRNGFMYGRG
EECCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCEEECCC
VDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESASTDLDKYLEKHADKLRGADL
CCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHCCHHHHHHHHHHHHHCCCEE
LVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIHSSYGGVVESAPWYLLQALQSLR
EEECCCHHHHHHHHEECCCCCEEEEEECEEEECCCEEECCCCCCHHCCHHHHHHHHHHHH
AADGRILVEGLYEEVQEPNEREMALLETYGQRNPEEVSRIYGLELPLLQEERMAFLKRFF
HCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHH
FEPALNIEGIQSGYQGQGVKTILPAEASAKLEVRLVPGLEPHDVLEKIRKQLDKNGFDKV
HCCCCCHHHHCCCCCCCCCEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCEE
ELYYTLGEMSYRSDMSAPAILNVIELAKKFYPQGVSVLPTTAGTGPMHTVFDALEVPMVA
EEEEEECCHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHCCHHE
FGLGNANSRDHGGDENVRIADYYTHIELVEELIRSYE
EECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA