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The map label for this gene is brnQ [C]

Identifier: 169833916

GI number: 169833916

Start: 667394

End: 668719

Strand: Direct

Name: brnQ [C]

Synonym: SPH_0719

Alternate gene names: 169833916

Gene position: 667394-668719 (Clockwise)

Preceding gene: 169834469

Following gene: 169832514

Centisome position: 29.72

GC content: 42.53

Gene sequence:

>1326_bases
ATGGCTAAAAAAGGTACCCTAACAGGTTTGCTCCTGTTTGGAATATTTTTTGGTGCGGGGAACTTGATTTTTCCGCCTTC
TCTAGGTGCTCTATCTGGAGAACATTTTCTTCCTGCCATCGCAGGTTTTGTCTTTTCAGGCGTTGGTATCGCCGTCTTGA
CCCTTATTATTGGAACGCTAAATCCTAAAGGATATATCTACGAGATTTCAACGAAGATAGCGCCTTGGTTTGCGACTCTT
TACCTCTCAGTTCTTTACTTGTCAATCGGTCCATTCTTTGCTACCCCACGTACTGCTACAACAGCTTACGAAGTAGGGAT
TAGCCCCCTTTTGTCGGATGCAAATAAAGGACTTGGCTTGATTGTATTTACGGTTCTGTATTTTGCGGCAGCCTATTTGA
TTTCGCTTAATCCATCAAAAATCTTAGACCGCATTGGACGTATTTTAACGCCAGTCTTTGCAATTTTGATTGTTATCTTG
GTCGTTCTGGGAGCTATCAAATATGGTGGAACAAGTCCTCAAGCTGCTTCAGCTGCTTATCAAGCTTCTGCCTTTGGTAC
AGGTTTCCTAGAAGGTTACAATACCTTGGACGCCCTTGCCTCAGTGGCCTTTAGCGTAATCGCAGTTCAAACCTTGAAAC
AACTTGGATTTTCAAGTAAGAAAGAATACATTTCAACTATTTGGGTTGTTGGTATCGTTGTTGCCCTTGCCTTCAGCGCT
CTTTACATCGGTTTAGGTTTTCTTGGAAATCATTTCCCAGTACCAGCTGAAGCGATGAAGGGTGGAACACCAGGTGTTTA
CATCTTGTCACAAGCCACTCAAGAAATCTTTGGCTCAACAGCTCAACTCTTCCTTGCAGCTATGGTTACCGTAACCTGCT
TCACAACGACTGTTGGTTTGATTGTGTCAACAGCTGAGTTCTTTAATGAGCGCTTCCCACAAATCAGCTACAAGGTTTAT
GCGACAGCCTTTACCTTGATTGGATTTGCTATTGCCAATTTGGGTCTTGATGCGATTATCAAGTACTCAATTCCAGTACT
GGTTATCTTGTACCCAATCACGATTGCTATCGTTATGATTGTCATTGTCAACAAATTTGTTGCCCTTTCAAAACCAGGTA
TGCAGTTGACAATTGCTGTGGTTACAGTTATTGCCATTGCAAGCGTACTAGGAAGCTCGTTTAAGGTTGAGTTTCTTGCA
AATCTTGTTAGCGTTCTTCCTTTTGCCAAGGCATCTCTCCCATGGTTGGTGCCAGCCATTGTTGGAATCTTGCTCTCATT
GGTTCTACCAAACAAGCAAGAAAGCGATGTTTTTGAAATGGAATAA

Upstream 100 bases:

>100_bases
AGAAAATTGAAAACATTTAATATTTGCCTCGTGATGCTTTTTTCAGACTCCTAATCGTGGTATACTAGGTCAGTATTTTA
TAAATATGAAGGAGATTTTT

Downstream 100 bases:

>100_bases
TCACTTAAATCACTTTTGTAGCCAAGTCTACAGGAGTGATTTTCTTTTTTTATCCGATGATAAATGTGTTATAATAGGTA
GCGAAAGAGGTGAAGAAATG

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFVFSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATL
YLSVLYLSIGPFFATPRTATTAYEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFSSKKEYISTIWVVGIVVALAFSA
LYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEIFGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVY
ATAFTLIGFAIANLGLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGSSFKVEFLA
NLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME

Sequences:

>Translated_441_residues
MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFVFSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATL
YLSVLYLSIGPFFATPRTATTAYEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFSSKKEYISTIWVVGIVVALAFSA
LYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEIFGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVY
ATAFTLIGFAIANLGLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGSSFKVEFLA
NLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME
>Mature_440_residues
AKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFVFSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLY
LSVLYLSIGPFFATPRTATTAYEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVILV
VLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFSSKKEYISTIWVVGIVVALAFSAL
YIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEIFGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYA
TAFTLIGFAIANLGLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGSSFKVEFLAN
LVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine), which is coupled to a proton motive force (Potential) [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=432, Percent_Identity=34.4907407407407, Blast_Score=222, Evalue=3e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 46651; Mature: 46520

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFVFSGVGIAVLTLIIGTL
CCCCCHHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
NPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTAYEVGISPLLSDANKGLGL
CCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCHHHHHHCCCCHHH
IVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVILVVLGAIKYGGTSPQAASAAY
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
QASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFSSKKEYISTIWVVGIVVALAFSA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEIFGSTAQLFLAAMVTVTCFTTTVGL
HHHHHHHHCCCCCCCHHHHCCCCCCEEEHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
IVSTAEFFNERFPQISYKVYATAFTLIGFAIANLGLDAIIKYSIPVLVILYPITIAIVMI
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
VIVNKFVALSKPGMQLTIAVVTVIAIASVLGSSFKVEFLANLVSVLPFAKASLPWLVPAI
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHH
VGILLSLVLPNKQESDVFEME
HHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
AKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFVFSGVGIAVLTLIIGTL
CCCCHHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC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HHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA