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The map label for this gene is uraA [H]

Identifier: 169833712

GI number: 169833712

Start: 1323215

End: 1324498

Strand: Direct

Name: uraA [H]

Synonym: SPH_1427

Alternate gene names: 169833712

Gene position: 1323215-1324498 (Clockwise)

Preceding gene: 169834467

Following gene: 169833253

Centisome position: 58.92

GC content: 42.83

Gene sequence:

>1284_bases
ATGAAACAAGAATCAACTGTTGATTTGTTACTTGACGTTGACCAACGTCCTTCGGCTGGAAAAGGAATTCTCCTTAGTTT
CCAACACGTTTTCGCCATGTTTGGTGCGACCATCTTGGTACCCTTAATTTTGGGAATGCCTGTATCTGTTGCCCTTTTTG
CTTCAGGTATTGGAACACTCATCTACATGATCGCAACTGGTTTTAGAGTTCCAGTTTATCTAGGATCATCATTCGCCTTT
ATCACAGCTATGTCCCTAGCTATGAAGGAACTAGGAGGGGATGTATCAGCTGCCCAAACAGGGGTTATCCTTACAGGTTT
TATCTATGTGCTTGTCGCTGCTGGTGTTCGTTTTGCGGGTACAAAATGGATTGATAAACTCTTGCCACCAATCATCATCG
GACCTATGATCATCGTTATCGGTCTTGGACTTGCAGGTTCAGCTGTTACAAACGCTGGTCTTGTAGCGGATGGAAATTGG
AAAAATGCACTTGTAGCAGTTGTTACTTTCTTGATTGCTGCCTTTATCAATACAAAAGGAAAAGGCTTCCTACGAATCAT
TCCATTCCTCTTTGCCATTATCGGTGGTTACCTCTTCGCACTAACCCTTGGCTTGGTTGACTTTACACCAGTTCTTAAAG
CCAACTGGTTTGAAATTCCTGGTTTCTACTTGCCATTTAGCACAGGTGGTGCCTTTAAAGAATACAATCTTTACTTTGGT
CCAGAAACTATCGCTATCTTGACAATCGCTATCGTAACAATTTCTGAACATATCGGAGACCATACTGTTTTGGGTCAAAT
CTGTGGCCGTCAATTCTTGAAAGAACCAGGTCTTCACCGTACTCTTCTTGGTGACGGTATCGCAACTTCAGTTTCTGCCT
TCCTTGGTGGACCAGCCAATACAACTTATGGAGAAAATACAGGGGTTATCGGTATGACTCGTATCGCTTCTGTCTCAGTT
ATCCGTAACGCTGCCTTTATCGCGATTGCCCTCAGCTTCCTTGGTAAATTCACTGCCTTGATTTCAACCATTCCAAATGC
TGTACTTGGTGGTATGTCAATCCTTCTCTACGGAGTTATCGCAAGTAATGGTTTGAAAGTCTTGATCAAAGAACGTGTAG
ACTTCAGTCAAATGCGTAACCTCATCATTGCAAGTGCTATGTTGGTCCTTGGACTTGGAGGAGCTATCCTTAAACTTGGT
CCAGTTACCCTTTCAGGTACTGCCCTATCAGCCATGACAGGAATCATCTTGAACTTGATCTTACCATACGAAAATAAAGA
CTAA

Upstream 100 bases:

>100_bases
TTAAAACTCTTTAAAATCTAGATTTTAGAGAAGTCTTACTCTGAGGGCCTATTGCTGTAAAATAATGGGCTCTTTTTTGA
TGCCCAAAAGTGAGGTTTAT

Downstream 100 bases:

>100_bases
GAATCTAAATACATCGAAAAACGAGCATTTCCAACTACGGAAGTGCTCATTTTTCTTCTTTCTAAAAAAGGAAAGCCCTG
CGGCCTTCCTTTGTCTTACT

Product: uracil permease

Products: Proton [Cytoplasm]; uracil [Cytoplasm] [C]

Alternate protein names: Uracil transporter [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLILGMPVSVALFASGIGTLIYMIATGFRVPVYLGSSFAF
ITAMSLAMKELGGDVSAAQTGVILTGFIYVLVAAGVRFAGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFEIPGFYLPFSTGGAFKEYNLYFG
PETIAILTIAIVTISEHIGDHTVLGQICGRQFLKEPGLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSV
IRNAAFIAIALSFLGKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFSQMRNLIIASAMLVLGLGGAILKLG
PVTLSGTALSAMTGIILNLILPYENKD

Sequences:

>Translated_427_residues
MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLILGMPVSVALFASGIGTLIYMIATGFRVPVYLGSSFAF
ITAMSLAMKELGGDVSAAQTGVILTGFIYVLVAAGVRFAGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFEIPGFYLPFSTGGAFKEYNLYFG
PETIAILTIAIVTISEHIGDHTVLGQICGRQFLKEPGLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSV
IRNAAFIAIALSFLGKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFSQMRNLIIASAMLVLGLGGAILKLG
PVTLSGTALSAMTGIILNLILPYENKD
>Mature_427_residues
MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLILGMPVSVALFASGIGTLIYMIATGFRVPVYLGSSFAF
ITAMSLAMKELGGDVSAAQTGVILTGFIYVLVAAGVRFAGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFEIPGFYLPFSTGGAFKEYNLYFG
PETIAILTIAIVTISEHIGDHTVLGQICGRQFLKEPGLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSV
IRNAAFIAIALSFLGKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFSQMRNLIIASAMLVLGLGGAILKLG
PVTLSGTALSAMTGIILNLILPYENKD

Specific function: Transport of uracil in the cell [H]

COG id: COG2233

COG function: function code F; Xanthine/uracil permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily [H]

Homologues:

Organism=Homo sapiens, GI44680145, Length=468, Percent_Identity=25.2136752136752, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI44680143, Length=465, Percent_Identity=25.5913978494624, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI44680148, Length=463, Percent_Identity=24.4060475161987, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI40316845, Length=463, Percent_Identity=24.4060475161987, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1788843, Length=413, Percent_Identity=42.3728813559322, Blast_Score=295, Evalue=4e-81,
Organism=Escherichia coli, GI87081818, Length=434, Percent_Identity=35.9447004608295, Blast_Score=233, Evalue=3e-62,
Organism=Escherichia coli, GI87082181, Length=451, Percent_Identity=27.2727272727273, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1790087, Length=473, Percent_Identity=26.8498942917548, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87082178, Length=471, Percent_Identity=25.2653927813163, Blast_Score=100, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17558856, Length=460, Percent_Identity=23.695652173913, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17541904, Length=452, Percent_Identity=22.5663716814159, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71993493, Length=433, Percent_Identity=23.7875288683603, Blast_Score=68, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17542262, Length=450, Percent_Identity=22.2222222222222, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI21356175, Length=450, Percent_Identity=24.2222222222222, Blast_Score=86, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006042
- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 44823; Mature: 44823

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS01116 XANTH_URACIL_PERMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLILGMPVSVALFASGIGTL
CCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
IYMIATGFRVPVYLGSSFAFITAMSLAMKELGGDVSAAQTGVILTGFIYVLVAAGVRFAG
HHHHHCCCEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHCCHHHH
TKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNWKNALVAVVTFLIAAFINTKG
HHHHHHHCCHHHHHHHHHHHHCCCCCCHHCCCCEEECCCCHHHHHHHHHHHHHHHHCCCC
KGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFEIPGFYLPFSTGGAFKEYNLYFG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCEEEECCCCCCCEECEEEEC
PETIAILTIAIVTISEHIGDHTVLGQICGRQFLKEPGLHRTLLGDGIATSVSAFLGGPAN
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHCCCCC
TTYGENTGVIGMTRIASVSVIRNAAFIAIALSFLGKFTALISTIPNAVLGGMSILLYGVI
CCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASNGLKVLIKERVDFSQMRNLIIASAMLVLGLGGAILKLGPVTLSGTALSAMTGIILNLI
HCCCCEEHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHEECCEEECCHHHHHHHHHHHHHH
LPYENKD
CCCCCCC
>Mature Secondary Structure
MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLILGMPVSVALFASGIGTL
CCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
IYMIATGFRVPVYLGSSFAFITAMSLAMKELGGDVSAAQTGVILTGFIYVLVAAGVRFAG
HHHHHCCCEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHCCHHHH
TKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNWKNALVAVVTFLIAAFINTKG
HHHHHHHCCHHHHHHHHHHHHCCCCCCHHCCCCEEECCCCHHHHHHHHHHHHHHHHCCCC
KGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFEIPGFYLPFSTGGAFKEYNLYFG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCEEEECCCCCCCEECEEEEC
PETIAILTIAIVTISEHIGDHTVLGQICGRQFLKEPGLHRTLLGDGIATSVSAFLGGPAN
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHCCCCC
TTYGENTGVIGMTRIASVSVIRNAAFIAIALSFLGKFTALISTIPNAVLGGMSILLYGVI
CCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASNGLKVLIKERVDFSQMRNLIIASAMLVLGLGGAILKLGPVTLSGTALSAMTGIILNLI
HCCCCEEHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHEECCEEECCHHHHHHHHHHHHHH
LPYENKD
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; uracil [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uracil [Periplasm] = Proton [Cytoplasm] + uracil [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8206848 [H]