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The map label for this gene is purF [H]

Identifier: 169833656

GI number: 169833656

Start: 128473

End: 129915

Strand: Direct

Name: purF [H]

Synonym: SPH_0153

Alternate gene names: 169833656

Gene position: 128473-129915 (Clockwise)

Preceding gene: 169834038

Following gene: 169833754

Centisome position: 5.72

GC content: 46.99

Gene sequence:

>1443_bases
ATGACATACGAAGTAAAATCTCTTAATGAAGAATGTGGTGTTTTCGGTATTTGGGGACATCCAGATGCTGCTAAGTTGAC
CTATTTTGGACTCCACAGTCTTCAACACCGTGGTCAGGAGGGGGCAGGAATCCTCTCCAATGATCAAGGACAACTGAAGC
GGCATCGCGATATGGGTCTTCTATCAGAAGTCTTCAGAAATCCTGCTAACTTAGATAAATTGACGGGAACTGGTGCGATT
GGGCATGTGCGTTATGCGACTGCTGGCGAAGCTTCTGTAGATAACATCCAGCCCTTCCTCTTCCGTTTTCACGATATGCA
GTTTGGCTTGGCTCATAATGGAAATCTGACCAATGCAGCCTCTCTCAAGAAAGAACTGGAACAAAGAGGAGCAATTTTCA
GCGCGACTTCGGACTCTGAAATCTTGGCTCACCTCATTCGTCGCAGTCATAATCCTAGCCTGATGGGCAAAATCAAGGAA
GCGCTCAGCCTTGTCAAAGGTGGTTTTGCCTATATCTTGCTGTTTGAGGACAAGTTGATTGCGGCTCTTGACCCTAATGG
CTTTCGTCCGCTTTCTATCGGGAAAATGGCCAACGGAGCGGTGGTTGTTTCCTCTGAAACCTGTGCTTTTGAGGTCATTG
GTGCCGAGTGGATTCGTGATTTGAAGCCAGGTGAGATTGTGATCATTGATGACGAGGGCATTCAGTATGACAGCTATACA
GATGATACCCAGTTGGCGGTTTGTTCTATGGAGTATATCTACTTTGCTCGCCCTGATTCTAATATCCACGGTGTCAATGT
CCATACGGCACGTAAGAGAATGGGAGCGCAATTGGCGCGAGAATTTAAGCATGAGGCAGATATTGTAGTTGGTGTGCCCA
ATTCTTCCCTAAGCGCGGCTATGGGATTTGCAGAAGAATCAGGCTTACCAAATGAAATGGGTCTGATCAAAAACCAATAC
ACCCAGCGAACTTTTATCCAACCGACTCAAGAATTGCGGGAGCAAGGAGTGCGGATGAAACTGTCTGCTGTTTCGGGTGT
TGTCAAAGGCAAACGTGTGGTCATGGTGGATGATTCCATTGTACGTGGAACAACCTCTCGTCGTATCGTTCAGCTCTTGA
AAGAAGCGGGTGCGACTGAGGTTCACGTTGCCATTGGAAGTCCTGCACTAGCGTATCCATGTTTCTACGGGATTGATATC
CAGACCCGTCAGGAGCTGATTGCAGCCAATCATACGGTCGAAGAAACTCGCCAAATCATTGGTGCGGACAGTCTGACTTA
TCTTTCAATTGATGGCTTGATTGAGTCGATTGGTATCGAAACAGATGCGCCGAACGGTGGTCTCTGTGTCGCTTACTTTG
ACGGTGACTACCCAACGCCTCTTTATGACTACGAAGAAGACTATCGTAGAAGTTTGGAAGAAAAGACCAGTTTTTACAAG
TAG

Upstream 100 bases:

>100_bases
GAAAATAAGACTTACAGATTTTCTAATAGATAGTATCAGTAATGTAAAAGTCATGTAAATCTAGCTCTTGATGATTACAA
ATGAAAATTAGGTATAAAAA

Downstream 100 bases:

>100_bases
GCGACAGATTCTCCATTAAAGAAAAGGAAAAAACAAATGACAAATAAAAATGCATATGCCCAGTCGGGTGTGGATGTTGA
AGCGGGTTATGAAGTTGTTG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGILSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGTGAI
GHVRYATAGEASVDNIQPFLFRFHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWIRDLKPGEIVIIDDEGIQYDSYT
DDTQLAVCSMEYIYFARPDSNIHGVNVHTARKRMGAQLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQY
TQRTFIQPTQELREQGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAYPCFYGIDI
QTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCVAYFDGDYPTPLYDYEEDYRRSLEEKTSFYK

Sequences:

>Translated_480_residues
MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGILSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGTGAI
GHVRYATAGEASVDNIQPFLFRFHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWIRDLKPGEIVIIDDEGIQYDSYT
DDTQLAVCSMEYIYFARPDSNIHGVNVHTARKRMGAQLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQY
TQRTFIQPTQELREQGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAYPCFYGIDI
QTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCVAYFDGDYPTPLYDYEEDYRRSLEEKTSFYK
>Mature_479_residues
TYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGILSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGTGAIG
HVRYATAGEASVDNIQPFLFRFHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKEA
LSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWIRDLKPGEIVIIDDEGIQYDSYTD
DTQLAVCSMEYIYFARPDSNIHGVNVHTARKRMGAQLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYT
QRTFIQPTQELREQGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAYPCFYGIDIQ
TRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCVAYFDGDYPTPLYDYEEDYRRSLEEKTSFYK

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=470, Percent_Identity=40.8510638297872, Blast_Score=335, Evalue=5e-92,
Organism=Escherichia coli, GI1788651, Length=476, Percent_Identity=38.8655462184874, Blast_Score=301, Evalue=4e-83,
Organism=Escherichia coli, GI1790167, Length=205, Percent_Identity=28.780487804878, Blast_Score=79, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17554892, Length=480, Percent_Identity=39.375, Blast_Score=312, Evalue=3e-85,
Organism=Saccharomyces cerevisiae, GI6323958, Length=503, Percent_Identity=36.3817097415507, Blast_Score=257, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24659598, Length=484, Percent_Identity=40.9090909090909, Blast_Score=346, Evalue=2e-95,
Organism=Drosophila melanogaster, GI28573187, Length=500, Percent_Identity=41, Blast_Score=343, Evalue=2e-94,
Organism=Drosophila melanogaster, GI24659604, Length=463, Percent_Identity=41.036717062635, Blast_Score=338, Evalue=6e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 52820; Mature: 52689

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGILSNDQGQLKRHRDMGL
CCEECCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
LSEVFRNPANLDKLTGTGAIGHVRYATAGEASVDNIQPFLFRFHDMQFGLAHNGNLTNAA
HHHHHCCCCCCHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHCCEECEECCCCCCHHH
SLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKEALSLVKGGFAYILLFEDKLI
HHHHHHHHCCCEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCEE
AALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWIRDLKPGEIVIIDDEGIQYDSYT
EEECCCCCCCEECCCCCCCEEEEECCCCEEEEECHHHHHCCCCCEEEEECCCCCEECCCC
DDTQLAVCSMEYIYFARPDSNIHGVNVHTARKRMGAQLAREFKHEADIVVGVPNSSLSAA
CCCEEEEEEEEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHH
MGFAEESGLPNEMGLIKNQYTQRTFIQPTQELREQGVRMKLSAVSGVVKGKRVVMVDDSI
HCCHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEEHHHHHHHCCCEEEEECCHH
VRGTTSRRIVQLLKEAGATEVHVAIGSPALAYPCFYGIDIQTRQELIAANHTVEETRQII
HCCCHHHHHHHHHHHCCCCEEEEEECCCHHHCCEEECCCHHHHHHHHHHCCCHHHHHHHH
GADSLTYLSIDGLIESIGIETDAPNGGLCVAYFDGDYPTPLYDYEEDYRRSLEEKTSFYK
CCCCEEEEEHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGILSNDQGQLKRHRDMGL
CEECCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
LSEVFRNPANLDKLTGTGAIGHVRYATAGEASVDNIQPFLFRFHDMQFGLAHNGNLTNAA
HHHHHCCCCCCHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHCCEECEECCCCCCHHH
SLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKEALSLVKGGFAYILLFEDKLI
HHHHHHHHCCCEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCEE
AALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWIRDLKPGEIVIIDDEGIQYDSYT
EEECCCCCCCEECCCCCCCEEEEECCCCEEEEECHHHHHCCCCCEEEEECCCCCEECCCC
DDTQLAVCSMEYIYFARPDSNIHGVNVHTARKRMGAQLAREFKHEADIVVGVPNSSLSAA
CCCEEEEEEEEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHH
MGFAEESGLPNEMGLIKNQYTQRTFIQPTQELREQGVRMKLSAVSGVVKGKRVVMVDDSI
HCCHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEEHHHHHHHCCCEEEEECCHH
VRGTTSRRIVQLLKEAGATEVHVAIGSPALAYPCFYGIDIQTRQELIAANHTVEETRQII
HCCCHHHHHHHHHHHCCCCEEEEEECCCHHHCCEEECCCHHHHHHHHHHCCCHHHHHHHH
GADSLTYLSIDGLIESIGIETDAPNGGLCVAYFDGDYPTPLYDYEEDYRRSLEEKTSFYK
CCCCEEEEEHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]