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The map label for this gene is dus1 [H]

Identifier: 169833585

GI number: 169833585

Start: 2192166

End: 2193146

Strand: Direct

Name: dus1 [H]

Synonym: SPH_2384

Alternate gene names: 169833585

Gene position: 2192166-2193146 (Clockwise)

Preceding gene: 169832972

Following gene: 169832561

Centisome position: 97.62

GC content: 48.11

Gene sequence:

>981_bases
GTGACAAATCTTAATACACCTTTTATGATTGGCAATGTTGAGATTCCCAATCGTACCGTTTTAGCGCCTATGGCTGGCGT
GACCAACTCAGCCTTTCGTACCATCGCAAAAGAGCTCGGAGCTGGACTCGTTGTAATGGAAATGGTCTCTGACAAGGGAA
TCCAATACAACAACGAAAAAACCCTGCACATGCTTCATATCGATGAGGGCGAAAACCCTGTCTCTATCCAACTTTTTGGT
AGCGATGAAGACAGCCTAGCACGCGCAGCAGAATTCATCCAAGAAAACACCAAGACCGATATCGTCGATATCAACATGGG
CTGCCCTGTCAACAAAATCGTGAAGAACGAAGCTGGTGCTATGTGGCTCAAGGATCCTGACAAGATCTACTCTATCATCA
ACAAGGTCCAGTCTGTCCTTGATATCCCACTTACTGTCAAAATGCGTACCGGCTGGGCGGACCCATCTCTTGCAGTAGAA
AATGCCCTCGCTGCTGAGGCTGCAGGTGTTTCTGCCCTCGCCATGCATGGCCGTACCCGTGAACAAATGTATACTGGCCA
CGCAGACCTTGAGACCCTTTACAAGGTTGCCCAAGCTCTAACCAAGATTCCATTCATCGCCAACGGTGATATCCGTACTG
TCCAAGAAGCCAAGCAACGCATCGAAGAAGTTGGTGCTGACGCAGTCATGATTGGCCGAGCTGCCATGGGAAATCCTTAC
CTCTTCAACCAAATCAACCATTACTTTGAAACAGGAGAAATCCTACCTGATTTGACCTTTGAAGACAAGATGAAGATCGC
CTACGAACACTTGAAACGATTGATTAACCTCAAAGGAGAAAACGTCGCAGTTCGTGAATTCCGCGGCCTCGCTCCTCACT
ATCTCCGTGGAACATCTGGCGCTGCCAAACTCCGTGGAGCCATTTCGCAAGCTAGCACCCTAGCAGAGATTGAAGCTCTC
TTGCAATTGGAAAAAGTATAA

Upstream 100 bases:

>100_bases
TTACAGGAAATGAAAGAGGAAGACCACGGGGCAGAAATCACTTGTCAATTCTGCCAAACTACTTACAACTTTGATGAAAA
GGACCTGGAGGAACTCATTC

Downstream 100 bases:

>100_bases
TGCAAAAAAGAGCCTAGGACAAAAGTATAATATTAAATATAAAAAGCGAACAAAATCAGTTATCTGACACTCAGAATTCT
GTCTTGTTCGCTTTTTTGTC

Product: tRNA-dihydrouridine synthase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLVVMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFG
SDEDSLARAAEFIQENTKTDIVDINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAKQRIEEVGADAVMIGRAAMGNPY
LFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLINLKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEAL
LQLEKV

Sequences:

>Translated_326_residues
MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLVVMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFG
SDEDSLARAAEFIQENTKTDIVDINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAKQRIEEVGADAVMIGRAAMGNPY
LFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLINLKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEAL
LQLEKV
>Mature_325_residues
TNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLVVMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGS
DEDSLARAAEFIQENTKTDIVDINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVEN
ALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAKQRIEEVGADAVMIGRAAMGNPYL
FNQINHYFETGEILPDLTFEDKMKIAYEHLKRLINLKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALL
QLEKV

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=256, Percent_Identity=32.03125, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI239788462, Length=243, Percent_Identity=31.6872427983539, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI31742496, Length=296, Percent_Identity=26.3513513513513, Blast_Score=98, Evalue=8e-21,
Organism=Homo sapiens, GI8923374, Length=249, Percent_Identity=32.1285140562249, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI40807366, Length=289, Percent_Identity=26.9896193771626, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1789660, Length=316, Percent_Identity=39.5569620253165, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI1788462, Length=243, Percent_Identity=34.5679012345679, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI145693211, Length=255, Percent_Identity=24.7058823529412, Blast_Score=78, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17543114, Length=252, Percent_Identity=29.3650793650794, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17507177, Length=239, Percent_Identity=28.0334728033473, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI25144369, Length=250, Percent_Identity=26.8, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17510279, Length=247, Percent_Identity=26.3157894736842, Blast_Score=88, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6323560, Length=252, Percent_Identity=30.1587301587302, Blast_Score=106, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6323433, Length=261, Percent_Identity=26.0536398467433, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323437, Length=229, Percent_Identity=24.0174672489083, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24585320, Length=236, Percent_Identity=32.2033898305085, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24580595, Length=231, Percent_Identity=29.8701298701299, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI19920448, Length=231, Percent_Identity=29.8701298701299, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI45549423, Length=246, Percent_Identity=31.7073170731707, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI19921524, Length=229, Percent_Identity=28.3842794759825, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35796; Mature: 35664

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00634 RIBOSOMAL_L30 ; PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLVVMEMVSDKGIQYNNEK
CCCCCCCEEEEEEECCCCEEECCCCCCCHHHHHHHHHHHCCCEEEEEHHHCCCCEECCCC
TLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIVDINMGCPVNKIVKNEAGA
EEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHCCCCE
MWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVENALAAEAAGVSALAMHGRTR
EEECCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHCCCCH
EQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAKQRIEEVGADAVMIGRAAMGNPY
HHHHCCCCCHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHCCCCEEEECCHHCCCCH
LFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLINLKGENVAVREFRGLAPHYLRGTSG
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCHHHCCCCC
AAKLRGAISQASTLAEIEALLQLEKV
HHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLVVMEMVSDKGIQYNNEK
CCCCCCEEEEEEECCCCEEECCCCCCCHHHHHHHHHHHCCCEEEEEHHHCCCCEECCCC
TLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIVDINMGCPVNKIVKNEAGA
EEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHCCCCE
MWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVENALAAEAAGVSALAMHGRTR
EEECCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHCCCCH
EQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAKQRIEEVGADAVMIGRAAMGNPY
HHHHCCCCCHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHCCCCEEEECCHHCCCCH
LFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLINLKGENVAVREFRGLAPHYLRGTSG
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCHHHCCCCC
AAKLRGAISQASTLAEIEALLQLEKV
HHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]