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The map label for this gene is gyrB [H]

Identifier: 169833521

GI number: 169833521

Start: 840616

End: 842562

Strand: Direct

Name: gyrB [H]

Synonym: SPH_0908

Alternate gene names: 169833521

Gene position: 840616-842562 (Clockwise)

Preceding gene: 169833171

Following gene: 169833880

Centisome position: 37.43

GC content: 42.84

Gene sequence:

>1947_bases
ATGACAGAAGAAATCAAAAATCTGCAGGCACAGGATTATGATGCCAGTCAAATTCAAGTTTTAGAGGGTTTAGAGGCTGT
TCGTATGCGTCCAGGGATGTACATTGGATCAACCTCAAAAGAAGGTCTTCACCATCTAGTCTGGGAAATTGTTGATAACT
CAATTGACGAGGCCTTGGCAGGATTTGCCAGCCATATTCAAGTTTTTATTGAGCCAGATGATTCGATTACTGTTGTGGAT
GATGGGCGTGGTATCCCAGTCGATATTCAGGAAAAAACAGGCCGTCCTGCTGTTGAGACCGTCTTTACAGTCCTTCATGC
TGGAGGAAAGTTCGGCGGTGGTGGATACAAGGTTTCAGGTGGCCTTCACGGGGTGGGGTCGTCAGTAGTTAATGCCCTTT
CCACTCAATTAGACGTTCATGTCCATAAAAACGGTAAGATTCATTACCAAGAATACCGTCGTGGTCATGTTGTTGCAGAC
CTTGAGGTGGTTGGGGATACAGATAGAACTGGAACAACGGTTCACTTCACACCGGACCCAGAAATTTTCACTGAAACAAC
AATCTTTGATTTTGATAAATTAAATAAACGGATTCAAGAGTTGGCCTTTCTAAATCGCGGTCTTCAAATTTCTATCACTG
ATAAGCGCCAAGGTTTGGAACAAACCAAGCATTATCATTATGAAGGTGGGATTGCTAGTTACGTTGAATATATCAACGAG
AACAAGGATGTAATCTTTGATACACCAATCTATACAGACGGTGAGATGGATGATATTACAGTTGAGGTAGCCATGCAGTA
CACAACAGGTTACCATGAAAATGTCATGAGTTTCGCCAATAATATTCATACCCATGAAGGTGGAACGCATGAACAAGGTT
TCCGTACGGCACTGACTCGTGTTATCAATGATTATGCTCGTAAGAATAAGTTACTGAAAGACAATGAAGATAATTTAACA
GGGGAAGATGTTCGCGAAGGCTTAACTGCAGTTATCTCAGTTAAACACCCAAATCCACAGTTTGAAGGACAAACCAAGAC
CAAATTGGGAAATAGCGAAGTGGTCAAGATTACCAATCGCCTCTTCAGTGAAGCCTTCTCCGATTTCCTCATGGAAAATC
CACAGATTGCCAAACGTATCGTAGAAAAAGGAATTTTGGCTGCCAAGGCTCGTGTGGCTGCCAAGCGTGCGCGTGAAGTC
ACACGTAAAAAATCTGGTTTGGAAATTTCCAACCTTCCAGGGAAACTAGCAGACTGTTCTTCTAATAACCCTGCTGAAAC
AGAACTCTTCATCGTCGAAGGAGACTCAGCTGGTGGATCAGCCAAATCTGGTCGTAACCGTGAGTTTCAGGCTATCCTTC
CAATTCGCGGTAAGATTTTGAATGTTGAAAAAGCAAGTATGGATAAGATTCTAGCCAACGAAGAAATTCGTAGTCTTTTC
ACAGCCATGGGAACAGGATTTGGCGCAGAATTTGATGTTTCGAAAGCCCGTTACCAAAAACTCGTTTTGATGACCGATGC
CGATGTCGATGGAGCCCACATTCGTACCCTTCTTTTAACCTTGATTTATCGTTATATGAAACCAATCCTAGAAGCTGGAT
ATGTTTATATTGCCCAACCACCAATCTATGGTGTCAAGGTTGGAAGCGAGATTAAAGAATATATCCAGCCGGGTGCAGAT
CAAGAAATCAAACTCCAAGAAGCTTTAGCCCGTTATAGTGAAGGTCGTACCAAACCGACTATTCAGCGTTATAAGGGGCT
AGGTGAAATGGACGATCATCAGCTGTGGGAAACAACCATGGATCCCGAACATCGCTTGATGGCTAGAGTTTCTGTAGATG
ATGCTGCAGAAGCAGATAAAATCTTTGATATGTTGATGGGGGATCGAGTAGAGCCTCGTCGTGAGTTTATCGAAGAAAAT
GCTGTCTATAGTACACTTGATGTCTAA

Upstream 100 bases:

>100_bases
TTGATATCGAAGCAGGTCAAGCTGCAGGACTTGATACCCACTTGTTTACCAGTATCGTGAATTTAAGACAAGTATTAGAC
ATATAAGAAAAAGGAATAAG

Downstream 100 bases:

>100_bases
AAAATAGGGAGAAGTAGTTCCCTTGGTCTAAAAAATATGATATAATCGTTACGATTGTTTCAAGTAAAAAAGGAGTTTGA
TATGTCTAATGGACAACTAA

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 648; Mature: 647

Protein sequence:

>648_residues
MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLHHLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVD
DGRGIPVDIQEKTGRPAVETVFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
LEVVGDTDRTGTTVHFTPDPEIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQGLEQTKHYHYEGGIASYVEYINE
NKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVMSFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLT
GEDVREGLTAVISVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARVAAKRAREV
TRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQAILPIRGKILNVEKASMDKILANEEIRSLF
TAMGTGFGAEFDVSKARYQKLVLMTDADVDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGAD
QEIKLQEALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDMLMGDRVEPRREFIEEN
AVYSTLDV

Sequences:

>Translated_648_residues
MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLHHLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVD
DGRGIPVDIQEKTGRPAVETVFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
LEVVGDTDRTGTTVHFTPDPEIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQGLEQTKHYHYEGGIASYVEYINE
NKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVMSFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLT
GEDVREGLTAVISVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARVAAKRAREV
TRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQAILPIRGKILNVEKASMDKILANEEIRSLF
TAMGTGFGAEFDVSKARYQKLVLMTDADVDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGAD
QEIKLQEALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDMLMGDRVEPRREFIEEN
AVYSTLDV
>Mature_647_residues
TEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLHHLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDD
GRGIPVDIQEKTGRPAVETVFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVADL
EVVGDTDRTGTTVHFTPDPEIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQGLEQTKHYHYEGGIASYVEYINEN
KDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVMSFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTG
EDVREGLTAVISVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARVAAKRAREVT
RKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQAILPIRGKILNVEKASMDKILANEEIRSLFT
AMGTGFGAEFDVSKARYQKLVLMTDADVDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQ
EIKLQEALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDMLMGDRVEPRREFIEENA
VYSTLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=678, Percent_Identity=25.5162241887906, Blast_Score=134, Evalue=4e-31,
Organism=Homo sapiens, GI19913406, Length=665, Percent_Identity=25.4135338345865, Blast_Score=122, Evalue=1e-27,
Organism=Escherichia coli, GI48994957, Length=565, Percent_Identity=53.6283185840708, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI1789408, Length=646, Percent_Identity=39.3188854489164, Blast_Score=441, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI212645845, Length=600, Percent_Identity=23.1666666666667, Blast_Score=130, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17535065, Length=676, Percent_Identity=24.7041420118343, Blast_Score=129, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI212645657, Length=235, Percent_Identity=25.531914893617, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=595, Percent_Identity=25.0420168067227, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI17136538, Length=693, Percent_Identity=25.1082251082251, Blast_Score=136, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 72253; Mature: 72122

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLHHLVWEIVDNSIDEALA
CCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHH
GFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVFTVLHAGGKFGGGGYKVSG
HHHHCEEEEECCCCCEEEEECCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEECC
GLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVADLEVVGDTDRTGTTVHFTPDP
CCCHHHHHHHHHHHHHEEEEEECCCCEEHHHHCCCCEEEEEEEECCCCCCCCEEEECCCC
EIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQGLEQTKHYHYEGGIASYVEYINE
CCEEECEEECHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHCEECCCCHHHHHHHHCC
NKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVMSFANNIHTHEGGTHEQGFRTALTR
CCCEEEECCCCCCCCCCCEEEEEEEEHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
VINDYARKNKLLKDNEDNLTGEDVREGLTAVISVKHPNPQFEGQTKTKLGNSEVVKITNR
HHHHHHHHCCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCHHHCCCCCHHHHHHHH
LFSEAFSDFLMENPQIAKRIVEKGILAAKARVAAKRAREVTRKKSGLEISNLPGKLADCS
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCC
SNNPAETELFIVEGDSAGGSAKSGRNREFQAILPIRGKILNVEKASMDKILANEEIRSLF
CCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEECCHHHHHHHHHHHHHHHHH
TAMGTGFGAEFDVSKARYQKLVLMTDADVDGAHIRTLLLTLIYRYMKPILEAGYVYIAQP
HHHCCCCCCCCCCCHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECC
PIYGVKVGSEIKEYIQPGADQEIKLQEALARYSEGRTKPTIQRYKGLGEMDDHQLWETTM
CCEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCHHHHCCC
DPEHRLMARVSVDDAAEADKIFDMLMGDRVEPRREFIEENAVYSTLDV
CHHHHHEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEECCC
>Mature Secondary Structure 
TEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLHHLVWEIVDNSIDEALA
CCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHH
GFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVFTVLHAGGKFGGGGYKVSG
HHHHCEEEEECCCCCEEEEECCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEECC
GLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVADLEVVGDTDRTGTTVHFTPDP
CCCHHHHHHHHHHHHHEEEEEECCCCEEHHHHCCCCEEEEEEEECCCCCCCCEEEECCCC
EIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQGLEQTKHYHYEGGIASYVEYINE
CCEEECEEECHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHCEECCCCHHHHHHHHCC
NKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVMSFANNIHTHEGGTHEQGFRTALTR
CCCEEEECCCCCCCCCCCEEEEEEEEHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
VINDYARKNKLLKDNEDNLTGEDVREGLTAVISVKHPNPQFEGQTKTKLGNSEVVKITNR
HHHHHHHHCCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCHHHCCCCCHHHHHHHH
LFSEAFSDFLMENPQIAKRIVEKGILAAKARVAAKRAREVTRKKSGLEISNLPGKLADCS
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCC
SNNPAETELFIVEGDSAGGSAKSGRNREFQAILPIRGKILNVEKASMDKILANEEIRSLF
CCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEECCHHHHHHHHHHHHHHHHH
TAMGTGFGAEFDVSKARYQKLVLMTDADVDGAHIRTLLLTLIYRYMKPILEAGYVYIAQP
HHHCCCCCCCCCCCHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECC
PIYGVKVGSEIKEYIQPGADQEIKLQEALARYSEGRTKPTIQRYKGLGEMDDHQLWETTM
CCEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCHHHHCCC
DPEHRLMARVSVDDAAEADKIFDMLMGDRVEPRREFIEENAVYSTLDV
CHHHHHEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7608096; 11463916 [H]