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The map label for this gene is yhcT [H]

Identifier: 169833510

GI number: 169833510

Start: 1990796

End: 1991632

Strand: Direct

Name: yhcT [H]

Synonym: SPH_2167

Alternate gene names: 169833510

Gene position: 1990796-1991632 (Clockwise)

Preceding gene: 169833584

Following gene: 169834394

Centisome position: 88.65

GC content: 39.67

Gene sequence:

>837_bases
ATGACGGTAAAGCAATTACTTGAGGAACAACTCCTCATCCCTAGAAAAATCCGTCATTTTTTGAGAATCAAGAAACATAT
TTTGATAAATCAAGAAGAAGTCCACTGGAACGAAATCGTAAATCCTGGAGATGTTTGCCAGTTGACTTTTGACGAGGAAG
ATTATTCCCAAAAGACGATCCCTTGGGGCAACCCAGACTTAGTGCAGGAAGTTTATCAAGATCAACACTTGATTATTGTA
AACAAACCAGAGGGGATGAAAACGCATGGTAATCAACCAAACGAAATTGCCCTTCTTAACCATGTCAGTACCTATGTTGG
CCAAACCTGCTATGTCGTTCATCGTCTGGACATGGAAACCAGTGGCTTAGTTCTCTTTGCCAAAAATCCTTTTATCCTGC
CCATTCTCAATCGCTTATTGGAGAAAAAAGAGATTTCTAGAGAATATTGGGCTCTAGTTGATGGAAATATCAACAGAAAA
GAACTTGTTTTCAGAGACAAAATTGGACGTGATCGCCATGATCGTAGAAAAAGAATAGTTGATGCAAAAAATGGGCAATA
TGCTGAAACGCATGTAAGCAGGTTAAAGCAATTCTCAAACAAGACTTCCTTGGCTCGTTGCAAGCTAAAGACAGGGCGAA
CCCATCAGATTCGTGTGCACCTTTCGCATCATAATCTTCCTATCCTGGGCGACCCTCTCTATAATAGTAAATCAAAGACA
AGTCGGCTTATGCTTCATGCCTTCCGACTTTCCTTTACCCACCCACTTACTTTAGAGAAGCTAACTTTCACTACCCTTTC
AAATACATTTGAAAAAGAATTAAAAAAGAATGGATGA

Upstream 100 bases:

>100_bases
AGGAAGACAAGAATTTAGCTATTTCCTATCCAAATAGGGCTTTTTTTGTTACAATATCTGTATGCAATTCACATTTACAT
TACCCGCCTCTCTACCTCAA

Downstream 100 bases:

>100_bases
TCGTGTCATCCATTTTTCCATATAAAAAGCAAGACCACAAAGCCTTGCTTTCTATCAACTCAAGAATTATTTAGCAATTT
TTGCGAAGTATTCAAGAGTA

Product: ribosomal large subunit pseudouridine synthase D

Products: NA

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGDVCQLTFDEEDYSQKTIPWGNPDLVQEVYQDQHLIIV
NKPEGMKTHGNQPNEIALLNHVSTYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
ELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLARCKLKTGRTHQIRVHLSHHNLPILGDPLYNSKSKT
SRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKKNG

Sequences:

>Translated_278_residues
MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGDVCQLTFDEEDYSQKTIPWGNPDLVQEVYQDQHLIIV
NKPEGMKTHGNQPNEIALLNHVSTYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
ELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLARCKLKTGRTHQIRVHLSHHNLPILGDPLYNSKSKT
SRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKKNG
>Mature_277_residues
TVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGDVCQLTFDEEDYSQKTIPWGNPDLVQEVYQDQHLIIVN
KPEGMKTHGNQPNEIALLNHVSTYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRKE
LVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLARCKLKTGRTHQIRVHLSHHNLPILGDPLYNSKSKTS
RLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKKNG

Specific function: Unknown

COG id: COG0564

COG function: function code J; Pseudouridylate synthases, 23S RNA-specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI23308689, Length=176, Percent_Identity=32.3863636363636, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI17158025, Length=202, Percent_Identity=27.7227722772277, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI221316663, Length=191, Percent_Identity=24.6073298429319, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1789155, Length=235, Percent_Identity=29.3617021276596, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1788946, Length=211, Percent_Identity=33.6492890995261, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786244, Length=221, Percent_Identity=30.7692307692308, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787327, Length=202, Percent_Identity=32.1782178217822, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17534965, Length=172, Percent_Identity=33.7209302325581, Blast_Score=70, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6324506, Length=226, Percent_Identity=26.5486725663717, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320168, Length=176, Percent_Identity=29.5454545454545, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321608, Length=188, Percent_Identity=26.5957446808511, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24583449, Length=174, Percent_Identity=36.2068965517241, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24583447, Length=174, Percent_Identity=36.2068965517241, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24583445, Length=174, Percent_Identity=36.2068965517241, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19921090, Length=174, Percent_Identity=30.4597701149425, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006225
- InterPro:   IPR006224
- InterPro:   IPR006145
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 32565; Mature: 32434

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: PS01129 PSI_RLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGDVCQLTFDEEDYSQKTI
CCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCEEEEEECCCCCCCCCC
PWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHVSTYVGQTCYVVHRLDMET
CCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEHHHHHHHHHCCEEEEEEEECCCC
SGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRKELVFRDKIGRDRHDRRKRIV
CCEEEEECCCEEHHHHHHHHHHHHHCCHHEEEECCCCCHHHHHHHHHCCCCHHHHHHHCC
DAKNGQYAETHVSRLKQFSNKTSLARCKLKTGRTHQIRVHLSHHNLPILGDPLYNSKSKT
CCCCCCHHHHHHHHHHHHHCCCHHHEEEECCCCEEEEEEEEECCCCCEECCCCCCCCHHH
SRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKKNG
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGDVCQLTFDEEDYSQKTI
CHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCEEEEEECCCCCCCCCC
PWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHVSTYVGQTCYVVHRLDMET
CCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEHHHHHHHHHCCEEEEEEEECCCC
SGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRKELVFRDKIGRDRHDRRKRIV
CCEEEEECCCEEHHHHHHHHHHHHHCCHHEEEECCCCCHHHHHHHHHCCCCHHHHHHHCC
DAKNGQYAETHVSRLKQFSNKTSLARCKLKTGRTHQIRVHLSHHNLPILGDPLYNSKSKT
CCCCCCHHHHHHHHHHHHHCCCHHHEEEECCCCEEEEEEEEECCCCCEECCCCCCCCHHH
SRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKKNG
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]