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The map label for this gene is xylS [H]

Identifier: 169833476

GI number: 169833476

Start: 372469

End: 374682

Strand: Direct

Name: xylS [H]

Synonym: SPH_0424

Alternate gene names: 169833476

Gene position: 372469-374682 (Clockwise)

Preceding gene: 169834404

Following gene: 169833327

Centisome position: 16.59

GC content: 35.91

Gene sequence:

>2214_bases
TTGAAAATTTTTAAGGGAGAGTTTTATCGAATCTCTGTATTAACAGACAAGCTAGTAAGGTTAGAATACTCTCGAACTGG
AAATTTTGAGGATAGAACGACACAACTTATCTATAATAGAGATTTTGGCCAAATTTCGTTAGATTATATCGAGACATCAA
ATGTACTAGATATTATGACGGACTATTTTCATCTGCACTTTAATAAAGGAGAATTTAACGCCGAAAATTTATTTATAGAA
TTAAAAGGAAATTTTGCCGTATATGGTAGTCGCTGGTATTTTGGTGAATCTATTGAAACGTTAAAAGGAACAGCTCGGAC
TCTGGATAAGGCAGATGGAGCAATCTCGTTAGAAGATGGAATTATTAGCCGAAATGGTATAGCCTTATTGGATGATTCTC
AAGGATTTATTTGGGATGAACAGTCTGGTTATATTGAGAGAGAAAATCAAATTGATCTGTATTTCTTTGCCTATGGGCAT
GATTATAGAGGAGCAATCAGAGACTTTTACCATTTGACTGGTTCAACACCCTTGTTGCCAAGATATGCTTTAGGCAATTG
GTGGAGTAGATATTGGCCTTATACGTCGGATGAATACTTGGATTTAATAGACAGATTTGAAACAGAGAAAATTCCATTAT
CTATCGGTGTGTTAGATATGGATTGGCATATAACTGACATTCCAGCTCGTTTTGGAAGTGGCTGGACAGGATATAGTTGG
AATAGAAACTTAATACCAAATCCAGAACAGTTATTGCAACAACTTCATGATAGAAAGTTAAAACTCTCTTTAAATGTCCA
TCCTGCTGATGGTATACGGGCTTATGAAGAAGCTTATCCTCAAGTCGCCAAACGATTAGGGTTAAATGTAGAACTAGAAG
AACCTGCCATTTTTGATTTTTTTAATCCCTCTTTTAGGGAAGCCTACTTTAAAGATGTTCATTATGAGCTAGAAAAGCAG
GGAGTAGATTTTTGGTGGATTGACTGGCAACAAGGGACTCAAGGCATATTGGATCCACTATGGCTTTTAAACCATTATCA
TTATCAGGATAGTTGTAAAAATGCAGAAGGTGGTTTGATTTTATCAAGATATGCAGGTCCTGGTAGCCACCGCTACCCTG
TTGGTTTTTCAGGGGACACTATTATTAGTTGGAATTCCTTAAGATTTCAACCTTATTTTACAGCGACAGCATCTAATATC
GGTTATAGTTGGTGGAGCCATGATATCGGTGGGCACATGCTGGGGGATTATGACGAAGAGCTGCAAACTAGATGGCTACA
GTTTGGTGTTTTTAGTCCGATCACTCGATTACATAGTTCTAGAAGTCCTTTTAATAGTAAAGAACCTTGGTTTTTTTCAG
AAACAACATCTAAGATTATGAAGAAATACCTTCGTTTGAGGCATCAGATGATTCCATATCTATACACTATGAATGTAAAG
ACACACGAGGAAGGTGCCCCATTAATCAGCCCAATGTATTATTTCTACCCAGAGAATGATGAGAGCTATAATGTTCCAAA
CCAATACTTTTTTGGAACAGAACTAATGGTGGCTCCCATTGTAGAAAAGATGGACTTGGCATTCCAATCTGCAAAAGTGG
ATGTATGGTTCCCTGAAGGTGAATGGTATGACTTCTTTTCAGAGAAAAAATATACAGGTGGTGTGAAGTTGAGTGTTTAT
AGAGACATTTCGACTATACCTGTGTTTGCAAAAAGTGGTGCGATCATTCCCTTGGTTGGTTCTGAGATAGATATGGGTGT
TGATTTACCTGAAGTTGTAGATTGGTATGTATTCCCAGGCAAACAACATTCTTTTGAAATGCTTGAAGATCAAAATGGTC
AAAGATATAAAACAAGATTATCAATCGATTGGGAAATGGGGATGGTAGAGTTAACATTACAAGGAGATTCTAGTATCGTT
CCAAGCAATAGAAGGCATAGAATTCATTTTAAAGGAACGAATGTGTCTATAATTGAATTACCAAATAAGAATGATACAGC
TAGATTTGAATGTAAAGATAATAAAACAATATCTTTAAATGATGAAGTTTTTAGACTACTAAAGACAGCTTCTCTTCCAT
ATGAATTAAAAGATAGATTGTTAAATCAATTCATCAATGCGAAAAACTCTCATGACTTAATGAATATCTTGCATCATCAG
GATAAGGAATTGAGAGGGCGTTTGTTGGAAATGATATTTACTAGCCAAAACTAA

Upstream 100 bases:

>100_bases
TCATAGATATAACAGATAGAACATTGCATTCAGAATAATGAAAAACGCATCAAAAATGGTGCGTTCTGTTTTTATGCTAC
CATAAAAGGAGGAAAACAAA

Downstream 100 bases:

>100_bases
TTGATATAATTAGAAAGTCATTTGTTTAGTGTATATGAAGATTCTGTATGGTATTCGTTATCACTGGTCACATTGAAAAT
GTGAAATAAGCACCTACAAC

Product: glycosy hydrolase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 737; Mature: 737

Protein sequence:

>737_residues
MKIFKGEFYRISVLTDKLVRLEYSRTGNFEDRTTQLIYNRDFGQISLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIE
LKGNFAVYGSRWYFGESIETLKGTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVLDMDWHITDIPARFGSGWTGYSW
NRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYEEAYPQVAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQ
GVDFWWIDWQQGTQGILDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPYFTATASNI
GYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPWFFSETTSKIMKKYLRLRHQMIPYLYTMNVK
THEEGAPLISPMYYFYPENDESYNVPNQYFFGTELMVAPIVEKMDLAFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVY
RDISTIPVFAKSGAIIPLVGSEIDMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDWEMGMVELTLQGDSSIV
PSNRRHRIHFKGTNVSIIELPNKNDTARFECKDNKTISLNDEVFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQ
DKELRGRLLEMIFTSQN

Sequences:

>Translated_737_residues
MKIFKGEFYRISVLTDKLVRLEYSRTGNFEDRTTQLIYNRDFGQISLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIE
LKGNFAVYGSRWYFGESIETLKGTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVLDMDWHITDIPARFGSGWTGYSW
NRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYEEAYPQVAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQ
GVDFWWIDWQQGTQGILDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPYFTATASNI
GYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPWFFSETTSKIMKKYLRLRHQMIPYLYTMNVK
THEEGAPLISPMYYFYPENDESYNVPNQYFFGTELMVAPIVEKMDLAFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVY
RDISTIPVFAKSGAIIPLVGSEIDMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDWEMGMVELTLQGDSSIV
PSNRRHRIHFKGTNVSIIELPNKNDTARFECKDNKTISLNDEVFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQ
DKELRGRLLEMIFTSQN
>Mature_737_residues
MKIFKGEFYRISVLTDKLVRLEYSRTGNFEDRTTQLIYNRDFGQISLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIE
LKGNFAVYGSRWYFGESIETLKGTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVLDMDWHITDIPARFGSGWTGYSW
NRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYEEAYPQVAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQ
GVDFWWIDWQQGTQGILDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPYFTATASNI
GYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPWFFSETTSKIMKKYLRLRHQMIPYLYTMNVK
THEEGAPLISPMYYFYPENDESYNVPNQYFFGTELMVAPIVEKMDLAFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVY
RDISTIPVFAKSGAIIPLVGSEIDMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDWEMGMVELTLQGDSSIV
PSNRRHRIHFKGTNVSIIELPNKNDTARFECKDNKTISLNDEVFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQ
DKELRGRLLEMIFTSQN

Specific function: Catalyzes the liberation of alpha-xylose from the non- reducing terminal glucose of xyloglucan oligosaccharides. Has high hydrolytic activity on the disaccharide isoprimeverose. Follows a retaining mechanism of substrate hydrolysis [H]

COG id: COG1501

COG function: function code G; Alpha-glucosidases, family 31 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 31 family [H]

Homologues:

Organism=Homo sapiens, GI66346737, Length=483, Percent_Identity=25.4658385093168, Blast_Score=158, Evalue=2e-38,
Organism=Homo sapiens, GI88900491, Length=487, Percent_Identity=25.0513347022587, Blast_Score=144, Evalue=3e-34,
Organism=Homo sapiens, GI38202257, Length=487, Percent_Identity=25.0513347022587, Blast_Score=144, Evalue=3e-34,
Organism=Homo sapiens, GI119393895, Length=557, Percent_Identity=22.9802513464991, Blast_Score=102, Evalue=2e-21,
Organism=Homo sapiens, GI119393893, Length=557, Percent_Identity=22.9802513464991, Blast_Score=102, Evalue=2e-21,
Organism=Homo sapiens, GI119393891, Length=557, Percent_Identity=22.9802513464991, Blast_Score=102, Evalue=2e-21,
Organism=Homo sapiens, GI310115361, Length=336, Percent_Identity=24.1071428571429, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI221316699, Length=336, Percent_Identity=24.1071428571429, Blast_Score=99, Evalue=2e-20,
Organism=Homo sapiens, GI157364974, Length=503, Percent_Identity=23.8568588469185, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI2367256, Length=634, Percent_Identity=21.4511041009464, Blast_Score=133, Evalue=4e-32,
Organism=Escherichia coli, GI2367323, Length=242, Percent_Identity=22.7272727272727, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17560798, Length=486, Percent_Identity=24.8971193415638, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17560800, Length=486, Percent_Identity=24.8971193415638, Blast_Score=132, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI71991189, Length=557, Percent_Identity=23.3393177737882, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71985706, Length=225, Percent_Identity=29.7777777777778, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI32563849, Length=226, Percent_Identity=26.5486725663717, Blast_Score=92, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6319706, Length=366, Percent_Identity=25.6830601092896, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24643749, Length=484, Percent_Identity=25, Blast_Score=112, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24643753, Length=484, Percent_Identity=25, Blast_Score=112, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24643751, Length=484, Percent_Identity=25, Blast_Score=112, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24643746, Length=484, Percent_Identity=25, Blast_Score=112, Evalue=9e-25,
Organism=Drosophila melanogaster, GI21357605, Length=484, Percent_Identity=25, Blast_Score=112, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24650054, Length=368, Percent_Identity=23.6413043478261, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011013
- InterPro:   IPR000322
- InterPro:   IPR017853 [H]

Pfam domain/function: PF01055 Glyco_hydro_31 [H]

EC number: NA

Molecular weight: Translated: 86138; Mature: 86138

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIFKGEFYRISVLTDKLVRLEYSRTGNFEDRTTQLIYNRDFGQISLDYIETSNVLDIMT
CCCCCCCEEEEEEEECEEEEEEECCCCCCCCCEEEEEEECCCCCEEEEEEECCCHHHHHC
DYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLKGTARTLDKADGAISLEDG
CEEEEEECCCCCCCCEEEEEEECCEEEEECEEEECCCHHHHHHHHHHHHHCCCEEEECCC
IISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGHDYRGAIRDFYHLTGSTPLLP
EECCCCEEEEECCCCCEEECCCCCEECCCCEEEEEEEECCCHHHHHHHHHHHCCCCCCCC
RYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVLDMDWHITDIPARFGSGWTGYSW
CHHHHCHHHHHCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCEEEECCHHHCCCCCCCCC
NRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYEEAYPQVAKRLGLNVELEEPAIFDF
CCCCCCCHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCEEEEC
FNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQGILDPLWLLNHYHYQDSCKNAEGGLI
CCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHCCCCCCCEE
LSRYAGPGSHRYPVGFSGDTIISWNSLRFQPYFTATASNIGYSWWSHDIGGHMLGDYDEE
EEECCCCCCCCCCCCCCCCEEEEECCEEEEEEEEEECCCCCCCHHCCCCCCCCCCCCHHH
LQTRWLQFGVFSPITRLHSSRSPFNSKEPWFFSETTSKIMKKYLRLRHQMIPYLYTMNVK
HHHHHHHHCCHHHHHHHHCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHCCEEEEEEEE
THEEGAPLISPMYYFYPENDESYNVPNQYFFGTELMVAPIVEKMDLAFQSAKVDVWFPEG
ECCCCCCEECCEEEEECCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHECCEEEEECCCC
EWYDFFSEKKYTGGVKLSVYRDISTIPVFAKSGAIIPLVGSEIDMGVDLPEVVDWYVFPG
CCCHHHCCCCCCCCEEEEEEECCCCCEEEECCCCEEEEECCCCCCCCCCHHHHEEEEECC
KQHSFEMLEDQNGQRYKTRLSIDWEMGMVELTLQGDSSIVPSNRRHRIHFKGTNVSIIEL
CCCCHHHHHCCCCCEEEEEEEEEEECCEEEEEECCCCCCCCCCCCEEEEEECCCEEEEEC
PNKNDTARFECKDNKTISLNDEVFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQ
CCCCCCEEEEECCCCEEEECHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCC
DKELRGRLLEMIFTSQN
CHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKIFKGEFYRISVLTDKLVRLEYSRTGNFEDRTTQLIYNRDFGQISLDYIETSNVLDIMT
CCCCCCCEEEEEEEECEEEEEEECCCCCCCCCEEEEEEECCCCCEEEEEEECCCHHHHHC
DYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLKGTARTLDKADGAISLEDG
CEEEEEECCCCCCCCEEEEEEECCEEEEECEEEECCCHHHHHHHHHHHHHCCCEEEECCC
IISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGHDYRGAIRDFYHLTGSTPLLP
EECCCCEEEEECCCCCEEECCCCCEECCCCEEEEEEEECCCHHHHHHHHHHHCCCCCCCC
RYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVLDMDWHITDIPARFGSGWTGYSW
CHHHHCHHHHHCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCEEEECCHHHCCCCCCCCC
NRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYEEAYPQVAKRLGLNVELEEPAIFDF
CCCCCCCHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCEEEEC
FNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQGILDPLWLLNHYHYQDSCKNAEGGLI
CCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHCCCCCCCEE
LSRYAGPGSHRYPVGFSGDTIISWNSLRFQPYFTATASNIGYSWWSHDIGGHMLGDYDEE
EEECCCCCCCCCCCCCCCCEEEEECCEEEEEEEEEECCCCCCCHHCCCCCCCCCCCCHHH
LQTRWLQFGVFSPITRLHSSRSPFNSKEPWFFSETTSKIMKKYLRLRHQMIPYLYTMNVK
HHHHHHHHCCHHHHHHHHCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHCCEEEEEEEE
THEEGAPLISPMYYFYPENDESYNVPNQYFFGTELMVAPIVEKMDLAFQSAKVDVWFPEG
ECCCCCCEECCEEEEECCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHECCEEEEECCCC
EWYDFFSEKKYTGGVKLSVYRDISTIPVFAKSGAIIPLVGSEIDMGVDLPEVVDWYVFPG
CCCHHHCCCCCCCCEEEEEEECCCCCEEEECCCCEEEEECCCCCCCCCCHHHHEEEEECC
KQHSFEMLEDQNGQRYKTRLSIDWEMGMVELTLQGDSSIVPSNRRHRIHFKGTNVSIIEL
CCCCHHHHHCCCCCEEEEEEEEEEECCEEEEEECCCCCCCCCCCCEEEEEECCCEEEEEC
PNKNDTARFECKDNKTISLNDEVFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQ
CCCCCCEEEEECCCCEEEECHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCC
DKELRGRLLEMIFTSQN
CHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10801892; 11427726 [H]