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The map label for this gene is hsdS [H]

Identifier: 169833432

GI number: 169833432

Start: 565346

End: 566626

Strand: Direct

Name: hsdS [H]

Synonym: SPH_0614

Alternate gene names: 169833432

Gene position: 565346-566626 (Clockwise)

Preceding gene: 169833785

Following gene: 169834025

Centisome position: 25.18

GC content: 32.4

Gene sequence:

>1281_bases
GTGAGGATAAAATCAATTTATTGGAATTTTGGGCAAAATAAGCCAGAGAAATCCTTTAGGTATATAGATACGTCTAGTAT
TGATAGAAAAAAGAACATAATCAACTACAAAAATCTACAATATCTTTCACCTGAACAAGCGCCTTCCCGTGCCAGAAAAT
TAGTTTCGCAGAATAGTGTCTTATTTTCAACAGTTAGACCATATCTAAAAAATATTGCTGTAGTTAGAGAACTTAAAGAG
TATTTGATAGCTAGTACAGCATTTATTGTTTTGGATACTTTACTTAACGAAACATATTTGAAATATTATTTATTATCAGA
TAATTTTATCAATCGTGTTAATAATAAATCTACTGGAACAAGTTATCCTGCAATCAATGATTATAATTTTAATCTATTAT
TAATTGCTCTCCCCCCACTATCCGAACAACAACGAATAGTAGAAGCAATCGAATCAGCTTTAGAAAAAGTAGATGAATAT
GCTGAAAGTTATAATAGACTAGAACAGCTAGATAAAGAATTTCCAGATAAACTAAAAAAATCTATTCTTCAATATGCTAT
GCAAGGAAAATTAGTTGAACAAGACCCAAATGATGAATCAGTCGAAGTTTTACTTGAAAAAATACGAGCAGAAAAACAAA
AACTCTTTGAAGAAGGCAAGATTAAAAAGAAAGATTTGGACATTTCTATTGTTTCCCAAGGAGATGATAACTCTTATTAT
GAGGAAGTACCTTGTGAAATACCAGAAAGTTGGGAATGGGTGAGGTTAAACGATATAACCTCCTACATTCAAAGAGGGAA
GTCTCCAAAATATTCAAATATTCCTATATACCCTGTGATTGCTCAGAAATGTAATCAATGGTCTGGCTTTTCTATTGACT
TAGCAAGATTTATTGATCCCGAAACAGTTCATAGTTACCAGAAAGAAAGATTACTAAGGGATGGTGATTTAATGTGGAAT
TCAACAGGTCTAGGTACGTTAGGACGATTAGCTATTTACCATGAAAATAAAAATCCTTATGGCTGGGCTGTTGCAGATAG
CCATGTTACAGTTATAAGAGTCTTATCGGGGGTTATTAATTGCCATTTCATCTATAATTTTCTTTCCTCTCCTATTGTTC
AGTCAGTTATTGAAGAAAAAGCGTCGGGTAGTACAAAACAAAAAGAATTATTAACGAAAACTATAAAAGAATATTTAATC
CCCCTCCCACCCCTTCCTGAACAATCCAGAATCGTTGATAAAATCGAACAATTTTTCGCCCATATTGACGCACTAATTTA
G

Upstream 100 bases:

>100_bases
TTATATACTTGAAGCACATAAGAAACATTATGGGAAGTTTGCTGATGGAAGTACTCAAGAAATTGATGTTCCTTATGATA
TTCCTGATACTTGGGAGTGG

Downstream 100 bases:

>100_bases
TATAATAGTTTTACGTGCGGTGGAATTTCTATCAAACATAAGATAGGAGTTTTACGTTGAAAGATTTTGAATTACATTTG
AAGAAAGCTGGTTTAGCTGA

Product: type I site-specific deoxyribonuclease chain S

Products: NA

Alternate protein names: S.EcoEI; Type I restriction enzyme EcoEI specificity protein; S protein [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MRIKSIYWNFGQNKPEKSFRYIDTSSIDRKKNIINYKNLQYLSPEQAPSRARKLVSQNSVLFSTVRPYLKNIAVVRELKE
YLIASTAFIVLDTLLNETYLKYYLLSDNFINRVNNKSTGTSYPAINDYNFNLLLIALPPLSEQQRIVEAIESALEKVDEY
AESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNSYY
EEVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCNQWSGFSIDLARFIDPETVHSYQKERLLRDGDLMWN
STGLGTLGRLAIYHENKNPYGWAVADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLI
PLPPLPEQSRIVDKIEQFFAHIDALI

Sequences:

>Translated_426_residues
MRIKSIYWNFGQNKPEKSFRYIDTSSIDRKKNIINYKNLQYLSPEQAPSRARKLVSQNSVLFSTVRPYLKNIAVVRELKE
YLIASTAFIVLDTLLNETYLKYYLLSDNFINRVNNKSTGTSYPAINDYNFNLLLIALPPLSEQQRIVEAIESALEKVDEY
AESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNSYY
EEVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCNQWSGFSIDLARFIDPETVHSYQKERLLRDGDLMWN
STGLGTLGRLAIYHENKNPYGWAVADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLI
PLPPLPEQSRIVDKIEQFFAHIDALI
>Mature_426_residues
MRIKSIYWNFGQNKPEKSFRYIDTSSIDRKKNIINYKNLQYLSPEQAPSRARKLVSQNSVLFSTVRPYLKNIAVVRELKE
YLIASTAFIVLDTLLNETYLKYYLLSDNFINRVNNKSTGTSYPAINDYNFNLLLIALPPLSEQQRIVEAIESALEKVDEY
AESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNSYY
EEVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCNQWSGFSIDLARFIDPETVHSYQKERLLRDGDLMWN
STGLGTLGRLAIYHENKNPYGWAVADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLI
PLPPLPEQSRIVDKIEQFFAHIDALI

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 49275; Mature: 49275

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIKSIYWNFGQNKPEKSFRYIDTSSIDRKKNIINYKNLQYLSPEQAPSRARKLVSQNSV
CCCCEEEECCCCCCCCCCEEECCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCH
LFSTVRPYLKNIAVVRELKEYLIASTAFIVLDTLLNETYLKYYLLSDNFINRVNNKSTGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHCCCCCCCC
SYPAINDYNFNLLLIALPPLSEQQRIVEAIESALEKVDEYAESYNRLEQLDKEFPDKLKK
CCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNSYY
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCH
EEVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCNQWSGFSIDLARFIDP
HHCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCEEHHHHCCH
ETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYGWAVADSHVTVIRVLSGVIN
HHHHHHHHHHHHHCCCEEECCCCCCCHHEEEEEECCCCCCEEEEECCHHHHHHHHHHHHH
CHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLIPLPPLPEQSRIVDKIEQFFA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
HIDALI
HHHHCC
>Mature Secondary Structure
MRIKSIYWNFGQNKPEKSFRYIDTSSIDRKKNIINYKNLQYLSPEQAPSRARKLVSQNSV
CCCCEEEECCCCCCCCCCEEECCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCH
LFSTVRPYLKNIAVVRELKEYLIASTAFIVLDTLLNETYLKYYLLSDNFINRVNNKSTGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHCCCCCCCC
SYPAINDYNFNLLLIALPPLSEQQRIVEAIESALEKVDEYAESYNRLEQLDKEFPDKLKK
CCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNSYY
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCH
EEVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCNQWSGFSIDLARFIDP
HHCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCEEHHHHCCH
ETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYGWAVADSHVTVIRVLSGVIN
HHHHHHHHHHHHHCCCEEECCCCCCCHHEEEEEECCCCCCEEEEECCHHHHHHHHHHHHH
CHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLIPLPPLPEQSRIVDKIEQFFA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
HIDALI
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2642743 [H]