Click here to switch to the map view.

The map label for this gene is capD [H]

Identifier: 169833402

GI number: 169833402

Start: 177001

End: 178851

Strand: Direct

Name: capD [H]

Synonym: SPH_0202

Alternate gene names: 169833402

Gene position: 177001-178851 (Clockwise)

Preceding gene: 169832797

Following gene: 169832419

Centisome position: 7.88

GC content: 42.03

Gene sequence:

>1851_bases
ATGAATAAAAAACTAACAGATTATGTGATTGATCTGGTGGAAATTTTAAATAAACAACAAAAGCAGGTTTTCTGGGGAAT
ATTTGATATTTTCAGTATGGTGGTTTCCATAATTGTATCTTATATTTTATTTTATGGGCTGATTAATCCAGCACCTGTTG
ACTACATTATCTATACGAGTTTGGCCTTCTTGTTCTATCAATTGATGATTGGTTTTTGGGGGTTGAACGCGAGCATTAGT
CGTTACAGCAAGATTACGGATTTCATGAAAATCTTTTTTGGTGTGACTGCTAGCAGTGTCTTGTCATATAGTATCTGTTA
TGCCTTCTTGCCACTCTTCTCCATCCGTTTCATCATTCTCTTTATCTTGTTGAGTACCTTCTTGATTTTATTGCCACGGA
TTACTTGGCAGTTAATCTACTCCAGACGCAAAAAAGGTAGTGGTGATGGAGAACACCGTCGGACCTTCTTGATTGGTGCC
GGTGATGGTGGGGCTCTCTTTATGGATAGTTACCAACATCCAACCAGTGAATTAGAACTGGTCGGTATTTTGGATAAGGA
TTCTAAGAAAAAGGGTCAAAAACTTGGTGGTATTCCTGTTTTGGGCTCTTATGACAATCTGCCTGAATTAGCCAAACGCC
ATCAAATCGAGCGTGTCATCGTTGCGATTCCGTCGCTGGATCCGTCAGAATATGAGCGTATCTTGCAGATGTGTAATAAG
CTGGGTGTCAAATGTTACAAGATGCCTAAGGTTGAAACTGTTGTTCAGGGCCTTCACCAAGCAGGTACTGGCTTCCAAAA
AATTGATATTACGGACCTTTTGGGTCGTCAGGAAATCCGTCTTGACGAATCGCGTCTGGGTGCAGAACTGACAGGTAAGA
CCATCTTAGTCACAGGAGCTGGAGGTTCAATCGGTTCTGAAATCTGTCGTCAAGTTAGTCGCTTCAATCCTGAACGCATT
GTCTTGCTCGGTCATGGGGAAAACTCAATCTACCTTGTTTATCATGAATTGATTCGTAAGTTCCAAGGGATTGATTATGT
ACCTGTGATTGCGGACATTCAAGACTATGATCGTTTGTTGCAAGTCTTTGAGCAGTACAAACCTGCTATTGTTTATCATG
CGGCAGCCCACAAGCATGTTCCTATGATGGAGCGCAATCCAAAAGAAGCCTTCAAAAACAATATCCGTGGAACTTACAAT
GTTGCTAAGGCTGTTGATGAAGCTAAAGTGTCTAAGATGGTTATGATTTCGACAGATAAGGCAGTTAATCCACCAAATGT
TATGGGAGCAACCAAGCGCGTGGCGGAGTTGATTGTCACTGGCTTTAACCAACGTAGCCAATCAACCTACTGTGCAGTTC
GTTTTGGGAATGTTCTTGGTAGCCGTGGTAGTGTCATTCCAGTCTTTGAACGTCAGATTGCTGAAGGTGGGCCTGTAACG
GTGACAGATTTCCGCATGACCCGTTACTTTATGACCATTCCAGAAGCTAGCCGTCTGGTTATCCATGCTGGTGCTTATGC
CAAAGATGGGGAAGTCTTTATCCTTGATATGGGTAAACCAGTCAAGATTTATGACTTGGCCAAGAAGATGGTGCTTCTAA
GTGGCCACACTGAAAGTGAAATTCCAATCGTTGAAGTTGGAATCCGCCCAGGTGAAAAACTCTACGAAGAACTCTTGGTA
TCAACCGAACTCGTTGACAATCAAGTTATGGATAAGATTTTCGTTGGTAAGGTTAATGTCATGCCTTTAGAATCCATCAA
TCAAAAGATTGGAGAGTTCCGCACTCTCAGTGGAGATGAGTTGAAGCAAGCTATTATCGCCTTTGCTAATCAAACAACCC
ACATTGAATAA

Upstream 100 bases:

>100_bases
ACAAGGGAACAATAATTTATTTGGATAAAATAAGCCAAATAAATCAAAATATGCTATAATAACAATATAGTAAAACTCTT
TTAAGGAGGAGTAGATTTCT

Downstream 100 bases:

>100_bases
AAAAGAAAAACGCATATTATCAAGTTAAATAACCTTGGTAATATGCGTTTTATTATGTAGAGACTTATACTCTTCGAAAA
TCTCTTCAAATCACGTCAAC

Product: CapD protein, required for the biosynthesis of type 1 capsular polysaccharide

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 616; Mature: 616

Protein sequence:

>616_residues
MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILFYGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASIS
RYSKITDFMKIFFGVTASSVLSYSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIERVIVAIPSLDPSEYERILQMCNK
LGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGRQEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERI
VLLGHGENSIYLVYHELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFKNNIRGTYN
VAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTYCAVRFGNVLGSRGSVIPVFERQIAEGGPVT
VTDFRMTRYFMTIPEASRLVIHAGAYAKDGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLV
STELVDNQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE

Sequences:

>Translated_616_residues
MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILFYGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASIS
RYSKITDFMKIFFGVTASSVLSYSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIERVIVAIPSLDPSEYERILQMCNK
LGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGRQEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERI
VLLGHGENSIYLVYHELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFKNNIRGTYN
VAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTYCAVRFGNVLGSRGSVIPVFERQIAEGGPVT
VTDFRMTRYFMTIPEASRLVIHAGAYAKDGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLV
STELVDNQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE
>Mature_616_residues
MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILFYGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASIS
RYSKITDFMKIFFGVTASSVLSYSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIERVIVAIPSLDPSEYERILQMCNK
LGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGRQEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERI
VLLGHGENSIYLVYHELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFKNNIRGTYN
VAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTYCAVRFGNVLGSRGSVIPVFERQIAEGGPVT
VTDFRMTRYFMTIPEASRLVIHAGAYAKDGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLV
STELVDNQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 69208; Mature: 69208

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILFYGLINPAPVDYIIYTS
CCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLSYSICYAFLPLFSIRFIIL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGAGDGGALFMDSYQHPTSELEL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCEEE
VGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIERVIVAIPSLDPSEYERILQMCNK
EEEECCCCHHCCCCCCCCCEECCCCCCHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHH
LGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGRQEIRLDESRLGAELTGKTILVTGA
CCCEEEECCCHHHHHHHHHHHCCCCEEECHHHHHCCHHHCCCHHHCCCEECCCEEEEECC
GGSIGSEICRQVSRFNPERIVLLGHGENSIYLVYHELIRKFQGIDYVPVIADIQDYDRLL
CCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEHHHHHHHHHCCCCCEEHHHCHHHHHHHH
QVFEQYKPAIVYHAAAHKHVPMMERNPKEAFKNNIRGTYNVAKAVDEAKVSKMVMISTDK
HHHHHCCCEEEEEEHHCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHCEEEEEECCC
AVNPPNVMGATKRVAELIVTGFNQRSQSTYCAVRFGNVLGSRGSVIPVFERQIAEGGPVT
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHCCCCCEEHHHHHHHCCCCCEE
VTDFRMTRYFMTIPEASRLVIHAGAYAKDGEVFILDMGKPVKIYDLAKKMVLLSGHTESE
EEEEEEEEEEEECCCCCEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHEEECCCCCC
IPIVEVGIRPGEKLYEELLVSTELVDNQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDE
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHCCCCHHH
LKQAIIAFANQTTHIE
HHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILFYGLINPAPVDYIIYTS
CCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLSYSICYAFLPLFSIRFIIL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGAGDGGALFMDSYQHPTSELEL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCEEE
VGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIERVIVAIPSLDPSEYERILQMCNK
EEEECCCCHHCCCCCCCCCEECCCCCCHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHH
LGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGRQEIRLDESRLGAELTGKTILVTGA
CCCEEEECCCHHHHHHHHHHHCCCCEEECHHHHHCCHHHCCCHHHCCCEECCCEEEEECC
GGSIGSEICRQVSRFNPERIVLLGHGENSIYLVYHELIRKFQGIDYVPVIADIQDYDRLL
CCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEHHHHHHHHHCCCCCEEHHHCHHHHHHHH
QVFEQYKPAIVYHAAAHKHVPMMERNPKEAFKNNIRGTYNVAKAVDEAKVSKMVMISTDK
HHHHHCCCEEEEEEHHCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHCEEEEEECCC
AVNPPNVMGATKRVAELIVTGFNQRSQSTYCAVRFGNVLGSRGSVIPVFERQIAEGGPVT
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHCCCCCEEHHHHHHHCCCCCEE
VTDFRMTRYFMTIPEASRLVIHAGAYAKDGEVFILDMGKPVKIYDLAKKMVLLSGHTESE
EEEEEEEEEEEECCCCCEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHEEECCCCCC
IPIVEVGIRPGEKLYEELLVSTELVDNQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDE
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHCCCCHHH
LKQAIIAFANQTTHIE
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]