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The map label for this gene is tcyA [H]

Identifier: 169833332

GI number: 169833332

Start: 227622

End: 228452

Strand: Direct

Name: tcyA [H]

Synonym: SPH_0260

Alternate gene names: 169833332

Gene position: 227622-228452 (Clockwise)

Preceding gene: 169832867

Following gene: 169833558

Centisome position: 10.14

GC content: 40.79

Gene sequence:

>831_bases
ATGAAAAAAATCGTTAAATACTCATCTCTTGCTGCCCTAGGACTTGTTGCTGCAGGTGTGCTTGCGGCTTGCTCAGGTGG
TGCTAAGAAAGAAGGAGAAGCAGCTAGCAAGAAAGAAATCATCGTTGCAACCAATGGATCACCAAGGCCATTTATCTATG
AAGAAAATGGCGAATTGACTGGTTACGAGATTGAAGTCGTTCGCGCTATCTTTAAAGATTCTGACAAATATGATGTCAAG
TTTGAAAAGACAGAATGGTCAGGTGTCTTTGCTGGTCTTGACGCTGACCGTTACAATATGGCTGTCAACAATCTTAGCTA
CACTAAAGAACGTGCGGAGAAATACCTCTATGCCGCACCAATTGCCCAAAATCCTAATGTCCTTGTCGTGAAGAAAGATG
ACTCTAGTATCAAGTCTCTCGATGATATCGGTGGAAAATCGACGGAAGTCGTTCAAGCCACTACATCAGCTAAGCAGTTA
GAAGCATACAACGCTGAACACACGGACAACCCAACTATCCTTAACTATACTAAGGCAGATTTCCAACAAATCATGGTACG
TTTGAGCGATGGACAATTTGACTATAAGATTTTTGATAAAATCGGTGTTGAAACAGTTATCAAGAACCAAGGTTTGGACA
ACTTGAAAGTTATCGAACTTCCAAGCGACCAACAACCGTACGTTTACCCACTTCTTGCTCAAGGTCAAGATGAGTTGAAA
TCATTTGTAGACAAACGCATTAAAGAACTCTACAAAGACGGAACGCTTGAAAAATTGTCTAAACAATTCTTCGGAGATAC
TTATCTACCAGCAGAGGCTGATATTAAATAA

Upstream 100 bases:

>100_bases
ACCCATAAAAAAAACTTATCACAGAGATAGATATCATATAATGGCGTAAATGCTCTTTTTCTGTTAAGATTATAAGGTAT
TCTATTTTGGAGGAAATGAC

Downstream 100 bases:

>100_bases
TTTCTTGAAATCATCCATTCTGAGTAAGATGGGTGATTTCTCAGTTTTTAGGGATATAGTTTTAAACTATATATCTGATT
ATCTAATAACAATTTGTGAA

Product: ABC transporter substrate-binding protein - amino acid transport

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVATNGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVK
FEKTEWSGVFAGLDADRYNMAVNNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVIELPSDQQPYVYPLLAQGQDELK
SFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK

Sequences:

>Translated_276_residues
MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVATNGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVK
FEKTEWSGVFAGLDADRYNMAVNNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVIELPSDQQPYVYPLLAQGQDELK
SFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
>Mature_276_residues
MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVATNGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVK
FEKTEWSGVFAGLDADRYNMAVNNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVIELPSDQQPYVYPLLAQGQDELK
SFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK

Specific function: Part of the ABC transporter complex tcyABC involved in L-cystine import [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=276, Percent_Identity=28.2608695652174, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787031, Length=267, Percent_Identity=25.0936329588015, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 30628; Mature: 30628

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVATNGSPRPFIYEENGELT
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCEEECCCCCCC
GYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAVNNLSYTKERAEKYLYAAP
CHHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCCHHHEECCCCHHHHHHHHHEEEECC
IAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQLEAYNAEHTDNPTILNYTKAD
CCCCCCEEEEECCCCHHHHHHHCCCCCCEEEEEHHHHHHHHHCCCCCCCCCCEEEECHHH
FQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVIELPSDQQPYVYPLLAQGQDELK
HHHHHHHHCCCCCCCHHHHHHCHHHHHHCCCCCCEEEEECCCCCCCEEEEEECCCHHHHH
SFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
HHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVATNGSPRPFIYEENGELT
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCEEECCCCCCC
GYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAVNNLSYTKERAEKYLYAAP
CHHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCCHHHEECCCCHHHHHHHHHEEEECC
IAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQLEAYNAEHTDNPTILNYTKAD
CCCCCCEEEEECCCCHHHHHHHCCCCCCEEEEEHHHHHHHHHCCCCCCCCCCEEEECHHH
FQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVIELPSDQQPYVYPLLAQGQDELK
HHHHHHHHCCCCCCCHHHHHHCHHHHHHCCCCCCEEEEECCCCCCCEEEEEECCCHHHHH
SFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
HHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7551042; 8969502; 9384377 [H]