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The map label for this gene is bbmA [H]

Identifier: 169833289

GI number: 169833289

Start: 1177785

End: 1179527

Strand: Direct

Name: bbmA [H]

Synonym: SPH_1270

Alternate gene names: 169833289

Gene position: 1177785-1179527 (Clockwise)

Preceding gene: 169833771

Following gene: 169832857

Centisome position: 52.45

GC content: 39.19

Gene sequence:

>1743_bases
GTGGAATTAACAGCCATTTACCACAGACCAGAGTCGGAGTATGCCTATCTTTATAAGGATAAGAAACTTCATATTCGGAT
TCGAACTAAGAAAGGGGATATTGAAAACATCAACTTGCATTATGGTGACCCTTTTATCTTTATGGAGGAGTTTTATCAAG
ATACAAAGAAAATGGCAAAGATAACTTCTGATGCCTTATTTGACCATTGGCAGGTTGAAGTGTCCGTCGACTTTGCACGT
ATCCAGTATCTATTTGAACTCAAGGATACAGAAGGTCAAAGTATTTTGTATGGCGATAAAGGGTGTGTTGAAAATTCTTT
AGAAAATCTTCGTGCTATTGGAAATGGATTTAAGTTGCCCTATCTTCATGAGATTGATGCCTGCAAGGTTCCTGACTGGG
TTTTAAATACGGTATGGTATCAGATATTTCCTGAGAGATTTGCCAATGGAAATGCTCTATTAAACCCAGAAGGGACATTA
GACTGGGATTTATCTATCACACCTCAGAGTGATGATTTCTTTGGTGGTGATTTACAGGGGATTATTGACCATCTGGATTA
TTTGCAAGACTTGGGCATTACTGGACTGTATCTTTGTCCTATCTTTGAATCTACGAGCAATCATAAGTACAATACGACAG
ATTACTTTGAAATTGACCGTCATTTTGGGGACAAGGATACTTTTCGGGAACTGGTCGAGCAAGCGCATCAGCGAGGTATG
AAAATTATGTTGGATGCAGTATTTAACCATATTGGTTCGCAATCGACTCAATGGCAAGATGTTGTCCAAAATGGTGAACA
GTCTGCTTATAAAGATTGGTTCCATATCCAACAATTCCCAGTGACTACTGAAAAGCCAGCTAATAAGAGAGACTTACCCT
ATCATGCTTTTGGTTTCGAGGACTATATGCCTAAGCTAAATACGGCCAATCCAGAAGTCAAGGACTATCTTTTAAAGGTT
GCGACTTATTGGATTGAAGAGTTTGATATCGATGCTTGGCGTTTGGATGTGGCTAATGAGATTGACCATCAGTTTTGGAA
GGATTTTCGAAAAGCAGTTTTAGCTAAAAAGCCAGATCTTTATATCCTAGGAGAAGTCTGGCATACGTCTCAGCCTTGGT
TAAATGGAGATGAGTTCCATGCGGTCATGAATTATCCTTTATCTGATAGTATCAAGGACTATTTCTTACGAGGAATTAAG
AAGACAGACCAATTCATCGATGAAATCAATTGCCAATCTATGTATTACAAGCAACAGATTTCAGAAGTTATGTTTAATCT
CTTGGATTCTCATGATACAGAGCGAATCCTGTGGACGGCTAATGAGGATGCTCAACTGGTCAAATCAGCTCTAGCCTTTC
TCTTTTTACAAAAGGGAACACCGTGTATTTATTACGGAACAGAGTTAGCTTTAACCGGAGGACCGGACCCAGATTGTCGA
CGTTGTATGCCTTGGGAACGTGTATCAAGTGACAATGAGATGCTGAACTTTATGAAGAGGCTGATTAAGATTCGCAAACA
TGCGTCAGCAACCATTCAGCATGGCAAGCATAGTCTCCAAGAAATCAAAGCTGATGTAGTAGCTTTGCAGTGGAAATATG
AAGGGCGAATCCTCAAAGCGATTTTCAACCAATCAAAGGAAGATTATTATTTACAAAAAGAAGCAGTAGCACTAGCAAGC
AATTGCCAAGAAATAGAGAATCAGCTTGTCATCTCTCCAAAAGGATTTGTGATTTTTCATTAA

Upstream 100 bases:

>100_bases
ACAAGCCTTGAAAATAAAATACGAAAGTAGTATACTAGAATCACAATTATGAAAACGTTTGCGTCGTAAATGCAACCAAG
TAAATCAGGAGACAAATATA

Downstream 100 bases:

>100_bases
AGAGCAATTAAAAGTGACATGAAAATTGTAGTATAGAGAGCATTTTACAAACAGACTATTTGTCGTCTTGCTACAAATGA
TATATAATCTAACTTAGAGT

Product: neopullulanase

Products: alpha-D-glucose; beta-D-glucose; amylose(n-1) [C]

Alternate protein names: NA

Number of amino acids: Translated: 580; Mature: 580

Protein sequence:

>580_residues
MELTAIYHRPESEYAYLYKDKKLHIRIRTKKGDIENINLHYGDPFIFMEEFYQDTKKMAKITSDALFDHWQVEVSVDFAR
IQYLFELKDTEGQSILYGDKGCVENSLENLRAIGNGFKLPYLHEIDACKVPDWVLNTVWYQIFPERFANGNALLNPEGTL
DWDLSITPQSDDFFGGDLQGIIDHLDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEIDRHFGDKDTFRELVEQAHQRGM
KIMLDAVFNHIGSQSTQWQDVVQNGEQSAYKDWFHIQQFPVTTEKPANKRDLPYHAFGFEDYMPKLNTANPEVKDYLLKV
ATYWIEEFDIDAWRLDVANEIDHQFWKDFRKAVLAKKPDLYILGEVWHTSQPWLNGDEFHAVMNYPLSDSIKDYFLRGIK
KTDQFIDEINCQSMYYKQQISEVMFNLLDSHDTERILWTANEDAQLVKSALAFLFLQKGTPCIYYGTELALTGGPDPDCR
RCMPWERVSSDNEMLNFMKRLIKIRKHASATIQHGKHSLQEIKADVVALQWKYEGRILKAIFNQSKEDYYLQKEAVALAS
NCQEIENQLVISPKGFVIFH

Sequences:

>Translated_580_residues
MELTAIYHRPESEYAYLYKDKKLHIRIRTKKGDIENINLHYGDPFIFMEEFYQDTKKMAKITSDALFDHWQVEVSVDFAR
IQYLFELKDTEGQSILYGDKGCVENSLENLRAIGNGFKLPYLHEIDACKVPDWVLNTVWYQIFPERFANGNALLNPEGTL
DWDLSITPQSDDFFGGDLQGIIDHLDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEIDRHFGDKDTFRELVEQAHQRGM
KIMLDAVFNHIGSQSTQWQDVVQNGEQSAYKDWFHIQQFPVTTEKPANKRDLPYHAFGFEDYMPKLNTANPEVKDYLLKV
ATYWIEEFDIDAWRLDVANEIDHQFWKDFRKAVLAKKPDLYILGEVWHTSQPWLNGDEFHAVMNYPLSDSIKDYFLRGIK
KTDQFIDEINCQSMYYKQQISEVMFNLLDSHDTERILWTANEDAQLVKSALAFLFLQKGTPCIYYGTELALTGGPDPDCR
RCMPWERVSSDNEMLNFMKRLIKIRKHASATIQHGKHSLQEIKADVVALQWKYEGRILKAIFNQSKEDYYLQKEAVALAS
NCQEIENQLVISPKGFVIFH
>Mature_580_residues
MELTAIYHRPESEYAYLYKDKKLHIRIRTKKGDIENINLHYGDPFIFMEEFYQDTKKMAKITSDALFDHWQVEVSVDFAR
IQYLFELKDTEGQSILYGDKGCVENSLENLRAIGNGFKLPYLHEIDACKVPDWVLNTVWYQIFPERFANGNALLNPEGTL
DWDLSITPQSDDFFGGDLQGIIDHLDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEIDRHFGDKDTFRELVEQAHQRGM
KIMLDAVFNHIGSQSTQWQDVVQNGEQSAYKDWFHIQQFPVTTEKPANKRDLPYHAFGFEDYMPKLNTANPEVKDYLLKV
ATYWIEEFDIDAWRLDVANEIDHQFWKDFRKAVLAKKPDLYILGEVWHTSQPWLNGDEFHAVMNYPLSDSIKDYFLRGIK
KTDQFIDEINCQSMYYKQQISEVMFNLLDSHDTERILWTANEDAQLVKSALAFLFLQKGTPCIYYGTELALTGGPDPDCR
RCMPWERVSSDNEMLNFMKRLIKIRKHASATIQHGKHSLQEIKADVVALQWKYEGRILKAIFNQSKEDYYLQKEAVALAS
NCQEIENQLVISPKGFVIFH

Specific function: Hydrolyzes beta-cyclodextrin to maltose and glucose, soluble starch to maltose and glucose, and pullulan to panose with trace amounts of maltose and glucose. It is also able to hydrolyze acarbose. Can also exhibit a transglycosylation activity transferrin

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family. BbmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=401, Percent_Identity=22.6932668329177, Blast_Score=73, Evalue=8e-13,
Organism=Escherichia coli, GI1786604, Length=490, Percent_Identity=29.7959183673469, Blast_Score=194, Evalue=2e-50,
Organism=Escherichia coli, GI1790687, Length=414, Percent_Identity=27.536231884058, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI1789995, Length=162, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6322245, Length=231, Percent_Identity=24.6753246753247, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321726, Length=287, Percent_Identity=23.3449477351916, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322241, Length=292, Percent_Identity=23.972602739726, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322021, Length=292, Percent_Identity=23.972602739726, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324416, Length=292, Percent_Identity=23.972602739726, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321731, Length=145, Percent_Identity=26.8965517241379, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319776, Length=145, Percent_Identity=26.8965517241379, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24586587, Length=230, Percent_Identity=27.8260869565217, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI221330053, Length=239, Percent_Identity=29.2887029288703, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24586599, Length=281, Percent_Identity=27.7580071174377, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24586597, Length=225, Percent_Identity=28.8888888888889, Blast_Score=84, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24583747, Length=228, Percent_Identity=29.3859649122807, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24583749, Length=228, Percent_Identity=29.3859649122807, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24586591, Length=225, Percent_Identity=26.2222222222222, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24583745, Length=225, Percent_Identity=27.5555555555556, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24586589, Length=256, Percent_Identity=27.34375, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45549022, Length=224, Percent_Identity=26.3392857142857, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24586593, Length=223, Percent_Identity=26.457399103139, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: 3.2.1.20 [C]

Molecular weight: Translated: 67823; Mature: 67823

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELTAIYHRPESEYAYLYKDKKLHIRIRTKKGDIENINLHYGDPFIFMEEFYQDTKKMAK
CCCEEEEECCCCCEEEEEECCEEEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHH
ITSDALFDHWQVEVSVDFARIQYLFELKDTEGQSILYGDKGCVENSLENLRAIGNGFKLP
HHHHHHHCCEEEEEEEEHHHEEEEEEECCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCC
YLHEIDACKVPDWVLNTVWYQIFPERFANGNALLNPEGTLDWDLSITPQSDDFFGGDLQG
CEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEEECCCCCCCCCCCHHH
IIDHLDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEIDRHFGDKDTFRELVEQAHQRGM
HHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCEEEEHHHCCCHHHHHHHHHHHHHCCH
KIMLDAVFNHIGSQSTQWQDVVQNGEQSAYKDWFHIQQFPVTTEKPANKRDLPYHAFGFE
HHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHEECCCCCCCCCCCCCCCEEECCHH
DYMPKLNTANPEVKDYLLKVATYWIEEFDIDAWRLDVANEIDHQFWKDFRKAVLAKKPDL
HHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCE
YILGEVWHTSQPWLNGDEFHAVMNYPLSDSIKDYFLRGIKKTDQFIDEINCQSMYYKQQI
EEEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
SEVMFNLLDSHDTERILWTANEDAQLVKSALAFLFLQKGTPCIYYGTELALTGGPDPDCR
HHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEECCEEEECCCCCCCHH
RCMPWERVSSDNEMLNFMKRLIKIRKHASATIQHGKHSLQEIKADVVALQWKYEGRILKA
HCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCHHHH
IFNQSKEDYYLQKEAVALASNCQEIENQLVISPKGFVIFH
HHCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEC
>Mature Secondary Structure
MELTAIYHRPESEYAYLYKDKKLHIRIRTKKGDIENINLHYGDPFIFMEEFYQDTKKMAK
CCCEEEEECCCCCEEEEEECCEEEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHH
ITSDALFDHWQVEVSVDFARIQYLFELKDTEGQSILYGDKGCVENSLENLRAIGNGFKLP
HHHHHHHCCEEEEEEEEHHHEEEEEEECCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCC
YLHEIDACKVPDWVLNTVWYQIFPERFANGNALLNPEGTLDWDLSITPQSDDFFGGDLQG
CEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEEECCCCCCCCCCCHHH
IIDHLDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEIDRHFGDKDTFRELVEQAHQRGM
HHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCEEEEHHHCCCHHHHHHHHHHHHHCCH
KIMLDAVFNHIGSQSTQWQDVVQNGEQSAYKDWFHIQQFPVTTEKPANKRDLPYHAFGFE
HHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHEECCCCCCCCCCCCCCCEEECCHH
DYMPKLNTANPEVKDYLLKVATYWIEEFDIDAWRLDVANEIDHQFWKDFRKAVLAKKPDL
HHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCCE
YILGEVWHTSQPWLNGDEFHAVMNYPLSDSIKDYFLRGIKKTDQFIDEINCQSMYYKQQI
EEEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
SEVMFNLLDSHDTERILWTANEDAQLVKSALAFLFLQKGTPCIYYGTELALTGGPDPDCR
HHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEECCEEEECCCCCCCHH
RCMPWERVSSDNEMLNFMKRLIKIRKHASATIQHGKHSLQEIKADVVALQWKYEGRILKA
HCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCHHHH
IFNQSKEDYYLQKEAVALASNCQEIENQLVISPKGFVIFH
HHCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): {} [C]

Substrates: Maltose; H2O; 1,4-alpha-D-glucan [C]

Specific reaction: Maltose + H2O = 2 alpha-D-glucose H2O + 1,4-alpha-D-glucan = beta-D-glucose + amylose(n-1) [C]

General reaction: O-Glycosyl bond hydrolysis; Exohydrolysis [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]