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The map label for this gene is argG [H]

Identifier: 169833085

GI number: 169833085

Start: 188464

End: 189660

Strand: Direct

Name: argG [H]

Synonym: SPH_0215

Alternate gene names: 169833085

Gene position: 188464-189660 (Clockwise)

Preceding gene: 169834421

Following gene: 169832670

Centisome position: 8.39

GC content: 41.52

Gene sequence:

>1197_bases
ATGAGTAAAGAAAAAGTAATTTTGGCCTATTCTGGCGGTTTGGACACATCAGTTGCTATTACCTGGTTAAAGAAAGACTA
TGATGTTGTTTCAGTTTGTATGGATGTGGGTGAAGGGAAAGACTTAGATTTCATCCATGATAAGGCTCTTAAGGTTGGGG
CAGTAGAATCTTATGTCATTGATGTTAAGGACGAATTTGCTACAGATTATGTGCTAGTGGCTCATCAGTCACATGCCTAT
TATGAACAAAAGTATCCCTTGGTATCTGCCTTGAGCCGCCCTCTGATTTCTAAAAAATTGGTTGAAATAGCGCATCAGAT
AGGAGCGACTACAATTGCTCATGGTTGTACCGGTAAGGGGAATGACCAAGTACGTTTTGAAGTATCGATTGCAGCCTTGG
ATCTCAATTTAAAAGTGATTGCTCCAGTTCGTGAATGGAAATGGTCTCGGGAGGAAGAAATTTATTATGCCAAGGAAAAT
GGGGTGCCTGTTCCTGCTGACCTTGACAATCCCTACTCTGTCGACCAAAATCTTTGGGGACGTGCCAATGAGTGTGGTAT
TTTGGAAAATCCATGGAATCAGGCTCCAGAAGAAGCCTTTGGCATCACAACTTCTCCAGAGCAAGCTCCAGATATGCCAG
AATACATTGAGATTGAGTTTAGTGAAGGTGTTCCAGTTTCCCTCAATGGAGAAGTGTTAAAATTGGCAGATTTGATTCAA
AAACTCAATGAAATAGCTGGAAAACATGGGGTTGGACGGATTGACCACGTGGAAAATCGCTTGGTTGGGATTAAATCAAG
AGAAATCTATGAGTGTCCAGGTGCAGTGACCCTGCTGACAGCTCATAAGGAAATTGAGGACTTGACGCTTGTGAGAGAAG
TGGCTCATTTTAAACCAATCATAGAAAATGAATTATCAAATCTCATTTATAATGCTTTGTGGTTTAGTTCGGCTACACAA
GCCTTGATTGCTTATATTAAAGAGACTCAAAAAGTTGTCAATGGGACTGCAAAAGTCAAACTGTATAAGGGGAGCGCTCA
GGTCGTGGCTCGGAAATCACCAAGTTCTCTTTACGATGAAAATTTGGCGACGTATACTAGTGCAGATACCTTTGACCAAG
ATGCGGCTGTTGGCTTTATTAAGTTATGGGGACTTCCAACCAAGGTTCATTCTGAGGTTCAAAAAAGCGCCAAATAG

Upstream 100 bases:

>100_bases
GATAAAACCATTCAAAAGTTGAAGGAGTCTGGGGAATTAGACAAACTCATTGAGGATGCCTTTAAAGCATCCATTGAAAA
ATAGAAAGAAGGAAAAGAAC

Downstream 100 bases:

>100_bases
GACGATTGGATAGAGAGGTGGATGTGATATGGCTAAGAATACAAAATTATGGGGTGGTCGCTTTGAAGGTACTGTCGAAG
ACTGGGTAGAGCGCTTTGGT

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MSKEKVILAYSGGLDTSVAITWLKKDYDVVSVCMDVGEGKDLDFIHDKALKVGAVESYVIDVKDEFATDYVLVAHQSHAY
YEQKYPLVSALSRPLISKKLVEIAHQIGATTIAHGCTGKGNDQVRFEVSIAALDLNLKVIAPVREWKWSREEEIYYAKEN
GVPVPADLDNPYSVDQNLWGRANECGILENPWNQAPEEAFGITTSPEQAPDMPEYIEIEFSEGVPVSLNGEVLKLADLIQ
KLNEIAGKHGVGRIDHVENRLVGIKSREIYECPGAVTLLTAHKEIEDLTLVREVAHFKPIIENELSNLIYNALWFSSATQ
ALIAYIKETQKVVNGTAKVKLYKGSAQVVARKSPSSLYDENLATYTSADTFDQDAAVGFIKLWGLPTKVHSEVQKSAK

Sequences:

>Translated_398_residues
MSKEKVILAYSGGLDTSVAITWLKKDYDVVSVCMDVGEGKDLDFIHDKALKVGAVESYVIDVKDEFATDYVLVAHQSHAY
YEQKYPLVSALSRPLISKKLVEIAHQIGATTIAHGCTGKGNDQVRFEVSIAALDLNLKVIAPVREWKWSREEEIYYAKEN
GVPVPADLDNPYSVDQNLWGRANECGILENPWNQAPEEAFGITTSPEQAPDMPEYIEIEFSEGVPVSLNGEVLKLADLIQ
KLNEIAGKHGVGRIDHVENRLVGIKSREIYECPGAVTLLTAHKEIEDLTLVREVAHFKPIIENELSNLIYNALWFSSATQ
ALIAYIKETQKVVNGTAKVKLYKGSAQVVARKSPSSLYDENLATYTSADTFDQDAAVGFIKLWGLPTKVHSEVQKSAK
>Mature_397_residues
SKEKVILAYSGGLDTSVAITWLKKDYDVVSVCMDVGEGKDLDFIHDKALKVGAVESYVIDVKDEFATDYVLVAHQSHAYY
EQKYPLVSALSRPLISKKLVEIAHQIGATTIAHGCTGKGNDQVRFEVSIAALDLNLKVIAPVREWKWSREEEIYYAKENG
VPVPADLDNPYSVDQNLWGRANECGILENPWNQAPEEAFGITTSPEQAPDMPEYIEIEFSEGVPVSLNGEVLKLADLIQK
LNEIAGKHGVGRIDHVENRLVGIKSREIYECPGAVTLLTAHKEIEDLTLVREVAHFKPIIENELSNLIYNALWFSSATQA
LIAYIKETQKVVNGTAKVKLYKGSAQVVARKSPSSLYDENLATYTSADTFDQDAAVGFIKLWGLPTKVHSEVQKSAK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=404, Percent_Identity=42.3267326732673, Blast_Score=302, Evalue=3e-82,
Organism=Homo sapiens, GI16950633, Length=404, Percent_Identity=42.3267326732673, Blast_Score=302, Evalue=3e-82,
Organism=Escherichia coli, GI1789563, Length=341, Percent_Identity=27.8592375366569, Blast_Score=113, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324514, Length=413, Percent_Identity=36.8038740920097, Blast_Score=263, Evalue=4e-71,
Organism=Drosophila melanogaster, GI21358151, Length=405, Percent_Identity=40, Blast_Score=282, Evalue=2e-76,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 44081; Mature: 43950

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEKVILAYSGGLDTSVAITWLKKDYDVVSVCMDVGEGKDLDFIHDKALKVGAVESYVI
CCCCCEEEEECCCCCCEEEEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHEECCEEEEEE
DVKDEFATDYVLVAHQSHAYYEQKYPLVSALSRPLISKKLVEIAHQIGATTIAHGCTGKG
ECCHHHCCCEEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCCC
NDQVRFEVSIAALDLNLKVIAPVREWKWSREEEIYYAKENGVPVPADLDNPYSVDQNLWG
CCEEEEEEEEEEEEECEEEEECHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHCC
RANECGILENPWNQAPEEAFGITTSPEQAPDMPEYIEIEFSEGVPVSLNGEVLKLADLIQ
CCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCEEEEEECCCCCEECCCCHHHHHHHHH
KLNEIAGKHGVGRIDHVENRLVGIKSREIYECPGAVTLLTAHKEIEDLTLVREVAHFKPI
HHHHHHCCCCCCCHHHHHHHEEECCCCCEEECCCCEEEEECHHHHHHHHHHHHHHHHHHH
IENELSNLIYNALWFSSATQALIAYIKETQKVVNGTAKVKLYKGSAQVVARKSPSSLYDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEEEECCCCHHHHC
NLATYTSADTFDQDAAVGFIKLWGLPTKVHSEVQKSAK
CCHHCCCCCCCCCCHHEEEEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SKEKVILAYSGGLDTSVAITWLKKDYDVVSVCMDVGEGKDLDFIHDKALKVGAVESYVI
CCCCEEEEECCCCCCEEEEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHEECCEEEEEE
DVKDEFATDYVLVAHQSHAYYEQKYPLVSALSRPLISKKLVEIAHQIGATTIAHGCTGKG
ECCHHHCCCEEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCCC
NDQVRFEVSIAALDLNLKVIAPVREWKWSREEEIYYAKENGVPVPADLDNPYSVDQNLWG
CCEEEEEEEEEEEEECEEEEECHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHCC
RANECGILENPWNQAPEEAFGITTSPEQAPDMPEYIEIEFSEGVPVSLNGEVLKLADLIQ
CCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCEEEEEECCCCCEECCCCHHHHHHHHH
KLNEIAGKHGVGRIDHVENRLVGIKSREIYECPGAVTLLTAHKEIEDLTLVREVAHFKPI
HHHHHHCCCCCCCHHHHHHHEEECCCCCEEECCCCEEEEECHHHHHHHHHHHHHHHHHHH
IENELSNLIYNALWFSSATQALIAYIKETQKVVNGTAKVKLYKGSAQVVARKSPSSLYDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEEEECCCCHHHHC
NLATYTSADTFDQDAAVGFIKLWGLPTKVHSEVQKSAK
CCHHCCCCCCCCCCHHEEEEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA