Click here to switch to the map view.

The map label for this gene is birA [H]

Identifier: 169832935

GI number: 169832935

Start: 1877060

End: 1877995

Strand: Direct

Name: birA [H]

Synonym: SPH_2017

Alternate gene names: 169832935

Gene position: 1877060-1877995 (Clockwise)

Preceding gene: 169833559

Following gene: 169834301

Centisome position: 83.59

GC content: 36.43

Gene sequence:

>936_bases
ATGAAATCCTACCAAGCTGTCTACCAAATCCTATCTAAAGAAACCGACTATATCAGCGGAGAAAAAATCGCAGAAAAACT
ATCCCTAAGCCGAACATCAATTTGGAAAGCCATCAAGCGACTAGAACAAGAAGGCATTGAAATTGATAGTATCAAAAATA
GAGGATATAAACTGATGAATGGTGACCTTATTCTTCCAGAGATTCTAGAAGAAAATCTTCCAATTAAAGTCAGCTTTAAA
CCCGAAACAAAATCAACACAACTAGATGCAAAAGAAGCAATTGATTTAGGCCATGAAGCAAATACCCTCTATCTAGCTTC
CTATCAAACAGCAGGCCGAGGCCGTTTTCAACGTTCCTTCTACTCACCACAAGGTGGTATTTATATGACACTCCATCTTA
AACCAAATCTCCCCTATGACAAATTACCATCCTACACACTACTTGTAGCTGGAGCTGTCTACAAAGCCATTAAGAACCTA
ACTTTAATAGATGTCGACATAAAATGGGTCAATGATATCTATCTAAACAATCATAAAATTGGAGGAATCCTTACTGAAGC
AATGACCTCTGTAGAAACTGGCTTAGTCACAGATATCATTATTGGAGTAGGTATCAATTTCACTATTAAAGACTTCCCTC
AGGAATTAAAAGAAAAAGCTGGCAGCTTATTTAAAGCTACAGCTCCTATAACAAGGAATGAATTGATCATAGAAATCTGG
CGTACTTTCTTCGAAACACCAGCAGAAGAGCTATTATACCTATACAAAAAACAGTCATTCATTCTAGGAAAAGAAGTCAC
TTTCACACTAGAGCAAAAAGACTACAAGGGATTTGCTAAAGACATCTCAGAAAATGGAAAACTTTTAGTTCAATGTGATA
ACGGAAAAGAAATCTGGCTAAATAGTGGCGAAATTTCTCTCAATAGTTGGAAGTAA

Upstream 100 bases:

>100_bases
CACTCCATTTTTCGAAAGCTTATAAACAATACTTTAATCAGACTCCAAGTCATACAAGAAAAGAATACTCTCAATACCAA
CTAGTAAGAAAGGCAACATT

Downstream 100 bases:

>100_bases
AATAACACAATTATAATATAAACGATATAAAAATAACTTCAGATTAGTAATTCAATTAAGTTTTACGGATCTGAAGTTTT
ATTGTCTCTAAAAACAAAAA

Product: biotin--protein ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQEGIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFK
PETKSTQLDAKEAIDLGHEANTLYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKEKAGSLFKATAPITRNELIIEIW
RTFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYKGFAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK

Sequences:

>Translated_311_residues
MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQEGIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFK
PETKSTQLDAKEAIDLGHEANTLYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKEKAGSLFKATAPITRNELIIEIW
RTFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYKGFAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK
>Mature_311_residues
MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQEGIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFK
PETKSTQLDAKEAIDLGHEANTLYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKEKAGSLFKATAPITRNELIIEIW
RTFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYKGFAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=312, Percent_Identity=27.8846153846154, Blast_Score=99, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 35294; Mature: 35294

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQEGIEIDSIKNRGYKLMN
CCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEEC
GDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANTLYLASYQTAGRGRFQRSF
CCEECHHHHHCCCCEEEEECCCCCCCCCCHHHHHHCCCCCCEEEEEECCCCCCCHHHHHH
YSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNLTLIDVDIKWVNDIYLNNHKI
CCCCCCEEEEEEECCCCCHHHCCCHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEECCCCC
GGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKEKAGSLFKATAPITRNELIIEIW
CHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHH
RTFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYKGFAKDISENGKLLVQCDNGKEIWL
HHHHHCCHHHHHHHHHHCCEEECCEEEEEEECCHHHHHHHHHCCCCCEEEEECCCCEEEE
NSGEISLNSWK
ECCEEEECCCC
>Mature Secondary Structure
MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQEGIEIDSIKNRGYKLMN
CCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEEC
GDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANTLYLASYQTAGRGRFQRSF
CCEECHHHHHCCCCEEEEECCCCCCCCCCHHHHHHCCCCCCEEEEEECCCCCCCHHHHHH
YSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNLTLIDVDIKWVNDIYLNNHKI
CCCCCCEEEEEEECCCCCHHHCCCHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEECCCCC
GGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKEKAGSLFKATAPITRNELIIEIW
CHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHH
RTFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYKGFAKDISENGKLLVQCDNGKEIWL
HHHHHCCHHHHHHHHHHCCEEECCEEEEEEECCHHHHHHHHHCCCCCEEEEECCCCEEEE
NSGEISLNSWK
ECCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]