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The map label for this gene is pmrA [H]

Identifier: 169832907

GI number: 169832907

Start: 989357

End: 990556

Strand: Direct

Name: pmrA [H]

Synonym: SPH_1073

Alternate gene names: 169832907

Gene position: 989357-990556 (Clockwise)

Preceding gene: 169833367

Following gene: 169833249

Centisome position: 44.06

GC content: 43.08

Gene sequence:

>1200_bases
ATGACAGAGATTAACTGGAAGGATAATCTGCGTATTGCCTGGTTTGGTAATTTTCTGACAGGAGCCAGTATTTCTTTGGT
TGTACCTTTTATGCCCATCTTCGTGGAAAATCTAGGTGTAGGGAGTCAGCAAGTCGCTTTTTATGCAGGCTTAGCAATTT
CTGTCTCTGCTATTTCCGCGGCGCTCTTTTCTCCTATTTGGGGTATTCTTGCTGACAAATACGGCCGAAAACCCATGATG
ATTCGGGCAGGTCTTGCTATGACTATCACTATGGGAGGCTTGGCCTTTGTCCCAAATATCTATTGGTTAATCTTTCTTCG
TTTACTAAACGGTGTATTTGCAGGTTTTGTTCCTAATGCAACGGCACTGATAGCCAGTCAGGTTCCAAAGGAGAAATCAG
GCTCTGCCTTAGGTACTTTGTCTACAGGCGTAGTTGCAGGTACTCTAACTGGTCCCTTTATTGGTGGCTTTATCGCAGAA
TTATTTGGCATTCGTACAGTTTTCTTACTGGTTGGTAGTTTTCTATTTTTAGCTGCTATTTTGACTATTTGCTTTATCAA
GGAAGATTTTCAACCAGTAGCCAAGGAAAAGGCTATTCCAACAAAGGAATTATTTACCTCGGTTAAATATCCCTATCTTT
TGCTCAATCTCTTTTTAACCAGTTTTGTCATCCAATTTTCAGCTCAATCGATCGGCCCTATTTTGGCTCTTTATGTACGC
GACTTAGGGCAGACAGAGAATCTTCTTTTTGTCTCTGGTTTGATTGTGTCCAGTATGGGCTTTTCCAGTATAATGAGTGC
AGGAGTCATGGGCAAGCTAGGTGACAAGGTGGGCAATCATCGTCTCTTGGTTGTCGCCCAGTTTTATTCAGTCATCATCT
ATCTCCTCTGTGCCAATGCCTCTAGCCCCCTTCAACTAGGACTCTATCGTTTCCTCTTTGGCTTGGGAACCGGTGCCTTG
ATTCCCGGGGTTAATGCCCTACTCAGCAAAATGACTCCCAAAGCCGGCATTTCGAGGGTCTTTGCCTTCAATCAGGTATT
CTTTTATCTGGGAGGTGTTGTTGGTCCCATGGCAGGTTCTGCAGTAGCAGGTCAATTTGGCTACCATGCTGTCTTTTATG
CGACAAGCCTTTGTGTTGCCTTTAGTTGTCTCTTTAACCTGATTCAATTTCGAACATTATTAAAAGTAAAGGAAATCTAG

Upstream 100 bases:

>100_bases
GAAAAAAAGAAAGATTTGGCCAGCCAGGTTCTTGATAATAATGGCAATCAGAACCAGCTTCTTAATCAAGTGCATATCCT
TCTTGAGGGAGGTAGGCAAG

Downstream 100 bases:

>100_bases
TGCGAGTAAAAATTAATCTCAAATGCTCCTCTTGTGACAGTATCAATTACCTAACCAGTAAAAATTCAAAAACCCATCCA
GACAAGATTGAGGTTTTAAA

Product: multidrug resistance protein MdtG

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQVAFYAGLAISVSAISAALFSPIWGILADKYGRKPMM
IRAGLAMTITMGGLAFVPNIYWLIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLFLTSFVIQFSAQSIGPILALYVR
DLGQTENLLFVSGLIVSSMGFSSIMSAGVMGKLGDKVGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGAL
IPGVNALLSKMTPKAGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRTLLKVKEI

Sequences:

>Translated_399_residues
MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQVAFYAGLAISVSAISAALFSPIWGILADKYGRKPMM
IRAGLAMTITMGGLAFVPNIYWLIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLFLTSFVIQFSAQSIGPILALYVR
DLGQTENLLFVSGLIVSSMGFSSIMSAGVMGKLGDKVGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGAL
IPGVNALLSKMTPKAGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRTLLKVKEI
>Mature_398_residues
TEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQVAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMI
RAGLAMTITMGGLAFVPNIYWLIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAEL
FGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLFLTSFVIQFSAQSIGPILALYVRD
LGQTENLLFVSGLIVSSMGFSSIMSAGVMGKLGDKVGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALI
PGVNALLSKMTPKAGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRTLLKVKEI

Specific function: Efflux pump for various substrates [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1787291, Length=392, Percent_Identity=44.8979591836735, Blast_Score=326, Evalue=2e-90,
Organism=Escherichia coli, GI87082404, Length=389, Percent_Identity=24.9357326478149, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 42822; Mature: 42691

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQVAFYAGLAISVSAISA
CCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
ALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYWLIFLRLLNGVFAGFVPNA
HHHHHHHHHHHHHHCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
TALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAELFGIRTVFLLVGSFLFLAAI
HHHHHHHCCCCCCCCCCEEHHHCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLFLTSFVIQFSAQSIGPILALYVR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLGQTENLLFVSGLIVSSMGFSSIMSAGVMGKLGDKVGNHRLLVVAQFYSVIIYLLCANA
HCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCC
SSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPKAGISRVFAFNQVFFYLGGVVGPMAGS
CCHHHHHHHHHHHHCCCCCHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
AVAGQFGYHAVFYATSLCVAFSCLFNLIQFRTLLKVKEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQVAFYAGLAISVSAISA
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
ALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYWLIFLRLLNGVFAGFVPNA
HHHHHHHHHHHHHHCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
TALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAELFGIRTVFLLVGSFLFLAAI
HHHHHHHCCCCCCCCCCEEHHHCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLFLTSFVIQFSAQSIGPILALYVR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLGQTENLLFVSGLIVSSMGFSSIMSAGVMGKLGDKVGNHRLLVVAQFYSVIIYLLCANA
HCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCC
SSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPKAGISRVFAFNQVFFYLGGVVGPMAGS
CCHHHHHHHHHHHHCCCCCHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
AVAGQFGYHAVFYATSLCVAFSCLFNLIQFRTLLKVKEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9869592; 11463916 [H]