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The map label for this gene is rlmN [H]

Identifier: 169832893

GI number: 169832893

Start: 805461

End: 806546

Strand: Direct

Name: rlmN [H]

Synonym: SPH_0867

Alternate gene names: 169832893

Gene position: 805461-806546 (Clockwise)

Preceding gene: 169834306

Following gene: 169833365

Centisome position: 35.87

GC content: 42.45

Gene sequence:

>1086_bases
ATGAAACCTTCAATTCATAGTTTGACACATCAAACCATGCAGGAATGGGTATTGGAGCAGGGAGAAAAGAAATTCCGTGC
AGATCAAATCTGGGAATGGCTCTACCGTAAACGTGTGCAGTCATTTGAAGAAATGACCAACCTTTCCAAGGATTTGATTG
CTAAGCTCAATGACCAGTTTGTGGTCAATCCCTTGAAACAACGTATTGTGCAAGAGTCTGCTGATGGTACAGTCAAATAT
CTCTTTGAATTGCCTGACGGTATGTTAATTGAGACTGTACTCATGCGTCAACACTATGGTTTATCTGTCTGTGTGACCAC
TCAGGTCGGCTGTAATATCGGTTGTACCTTCTGTGCCTCTGGTTTGATTAAGAAGCAGCGTGACCTCAATAACGGTGAAA
TCGTAGCGCAAATCATGCTGGTTCAGAAATACTTTGATGAGCGTGGTCAGGATGAACGCGTCAGCCATATCGTTGTCATG
GGAATCGGTGAGCCCTTTGATAATTACAACAATGTCTTGAATTTCTTTCGTACTATCAATGATGATAAAGGAATGGCTAT
CGGTGCTCGTCACATCACGGTTTCAACCTTAGGTTTGGCTCATAAAATTCGTGACTTTGCTGATGAAGGGGTTCAGGTCA
ATCTTGCCGTGTCTCTTCACGCACCTAACAATGAACTTCGTTCAAGTATCATGAAGATTAACCGTGCCTTTCCGATTGAA
AAACTCTTTGCTGCTATTGAATACTACATTGAGACAACAAACCGTCGTGTAACCTTTGAATATATCATGCTTAATGAAGT
CAACGACGGTGTAGAACAAGCCTTGGAATTGGCTGAATTGCTCAAGAACATCAAGAAATTGTCTTATGTAAACTTGATTT
CTTATAACCCAGTTAGTGAGCATGACCAATATAGCCGTAGTCCCAAAGAGCGCGTGTTGGCTTTCTATGATACGCTTAAG
AAAAAAGGGGGCAACTGTGTGGTTCGTCAAGAGCATGGTACAGATATTGACGCAGCTTGTGGACAATTGCGCTCTAACAC
AATGAAACGTGACCGCCAGAAGGCAGTTGCAGCGGTAAATCCTTAA

Upstream 100 bases:

>100_bases
AAAATAGACGTCGTCAGAAAGACCAGCATTCTCAGAAAGAGGACAAGGGACAACGTCATTTTGTCATTCGTCAGAAGTGA
GTAGAGAATAAGGAGAAGAA

Downstream 100 bases:

>100_bases
TGATGAAGAAGACTTATAATCATATTTTGGTCTGGGGAGTCATTTTCTATAGCATTTGCATTGTCTGTTTTTGCTTTACT
CCTCAAGAACAATCTACCGT

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MKPSIHSLTHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEEMTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKY
LFELPDGMLIETVLMRQHYGLSVCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERVSHIVVM
GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSTLGLAHKIRDFADEGVQVNLAVSLHAPNNELRSSIMKINRAFPIE
KLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALELAELLKNIKKLSYVNLISYNPVSEHDQYSRSPKERVLAFYDTLK
KKGGNCVVRQEHGTDIDAACGQLRSNTMKRDRQKAVAAVNP

Sequences:

>Translated_361_residues
MKPSIHSLTHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEEMTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKY
LFELPDGMLIETVLMRQHYGLSVCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERVSHIVVM
GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSTLGLAHKIRDFADEGVQVNLAVSLHAPNNELRSSIMKINRAFPIE
KLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALELAELLKNIKKLSYVNLISYNPVSEHDQYSRSPKERVLAFYDTLK
KKGGNCVVRQEHGTDIDAACGQLRSNTMKRDRQKAVAAVNP
>Mature_361_residues
MKPSIHSLTHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEEMTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKY
LFELPDGMLIETVLMRQHYGLSVCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERVSHIVVM
GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSTLGLAHKIRDFADEGVQVNLAVSLHAPNNELRSSIMKINRAFPIE
KLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALELAELLKNIKKLSYVNLISYNPVSEHDQYSRSPKERVLAFYDTLK
KKGGNCVVRQEHGTDIDAACGQLRSNTMKRDRQKAVAAVNP

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=358, Percent_Identity=37.4301675977654, Blast_Score=233, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 41269; Mature: 41269

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSIHSLTHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEEMTNLSKDLIAKLNDQF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLSVCVTTQVGCNIGCTFCAS
EECHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHH
GLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERVSHIVVMGIGEPFDNYNNVLNFFRTIN
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCC
DDKGMAIGARHITVSTLGLAHKIRDFADEGVQVNLAVSLHAPNNELRSSIMKINRAFPIE
CCCCCEEEEHEEEHHHHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHHCCCHH
KLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALELAELLKNIKKLSYVNLISYNPVSE
HHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCC
HDQYSRSPKERVLAFYDTLKKKGGNCVVRQEHGTDIDAACGQLRSNTMKRDRQKAVAAVN
CHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
P
C
>Mature Secondary Structure
MKPSIHSLTHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEEMTNLSKDLIAKLNDQF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLSVCVTTQVGCNIGCTFCAS
EECHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHH
GLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERVSHIVVMGIGEPFDNYNNVLNFFRTIN
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCC
DDKGMAIGARHITVSTLGLAHKIRDFADEGVQVNLAVSLHAPNNELRSSIMKINRAFPIE
CCCCCEEEEHEEEHHHHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHHCCCHH
KLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALELAELLKNIKKLSYVNLISYNPVSE
HHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCC
HDQYSRSPKERVLAFYDTLKKKGGNCVVRQEHGTDIDAACGQLRSNTMKRDRQKAVAAVN
CHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
P
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA