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The map label for this gene is rumA [C]

Identifier: 169832868

GI number: 169832868

Start: 1040602

End: 1042233

Strand: Direct

Name: rumA [C]

Synonym: SPH_1131

Alternate gene names: 169832868

Gene position: 1040602-1042233 (Clockwise)

Preceding gene: 169832636

Following gene: 169833789

Centisome position: 46.34

GC content: 40.75

Gene sequence:

>1632_bases
ATGTTAAAGAAAAATGATATTGTAGAAGTTGAAATTGTTGATTTGACCCATGAAGGGGCAGGAGTTGCCAAGGTAGATGG
TTTGGTCTTTTTTGTAGAGAATGCTTTACCGAGTGAAAAAATTCTCATGCGTGTCCTCAAGGTCAATAAAAAGATTGGCT
TTGGAAAAGTTGAAAAATACCTTGTCCAGTCACCACACCGTAATCAAGATCTAGATTTGGCTTACCTGCGTTCAGGAATC
GCGGATTTAGGACACCTTTCTTATCCAGAACAGCTCAAGTTTAAAACCAAGCAAGTCAAGGACAGTCTCTACAAGATTGC
TGGAATTGCAGATGTAGAAGTTGCTGAAACGCTTGGTATGGAACATCCAGTCAAGTATCGCAATAAGGCGCAGGTGCCCG
TTCGTCGAGTGAATGGTGTCTTGGAAACAGGATTTTTCCGTAAGAATTCGCACAACCTCATGCCCCTTGAAGATTTCTTT
ATACAGGATCCTGTCATTGACCAAGTCGTAGTAGCTCTTCGAGACCTGCTCCGTCGTTTTGATTTAAAACCTTATGACGA
AAAGGAACAGTCTGGATTGATTCGGAATCTTGTGGTGCGTCGTGGTCACTATTCAGGACAAATCATGGTCGTTTTGGTGG
CAACTCGTCCAAAAGTTTTTCGTGTTGACCAATTGATTGAACAAGTTATCAAGCAGTTCCCAGAGATTGTGTCTGTCATG
CAAAATATCAACGACCAGAATACCAATGCGATTTTTGGTAAGGAGTGGCGCACTCTTTATGGTCAAGACTATATTACGGA
CCAGATGTTGGGAAATGACTTCCAAATCGCTGGCCCAGCCTTTTACCAAGTCAATACTGAAATGGCGGAGAAACTCTATC
AAACAGCCATTGACTTTGCAGAGTTAAAAAAAGATGACGTGGTTATTGATGCTTATTCTGGTATTGGAACCATTGGTTTA
TCAGTCGCCAAGCATGTCAAAGAAGTCTACGGTGTTGAACTGATTCCAGAAGCGGTTGAGAATAGTAAAAAAAATGCTCA
GTTGAACAATATTTCAAACGCCCACTATGTCTGTGACACAGCTGAAAATGCTATGAAGAATTGGCTTAAAGATGGGATTC
AACCAACCGTTATCTTGGTTGATCCTCCACGCAAGGGCTTGACAGAAAGCTTTATCAAAGCAAGCGCCCAAACAGGAGCC
GATCGCATCGCCTATATCTCCTGCAATGTCGCAACCATGGCGCGTGATATCAAACTCTACCAAGAATTGGGATATGAATT
GAAGAAAGTCCAGCCGGTGGATCTATTTCCTCAAACGCATCACGTCGAGACGGTAGCACTTTTGTCCAAACTCGATGTCG
ATAAGCACATAAGTGTTGAAATTGAGCTGGATGAGATGAATTTGACAAGTGCGGAGAGCAAAGCAACATATGCTCAAATC
AAAGAATATGTTTGGAATAAATTTGAATTAAAAGTTTCGACATTATATATTGCACAGATAAAAAAGAAATGTGGAATAGA
ATTACGAGAACATTACAACAAGTCTAAAAAGGATAAACAAATTATTCCACAGTGTACACCTGAAAAAGAAGAAGCCATCA
TGGATGCTTTGAGACACTTCAAAATGATTTAA

Upstream 100 bases:

>100_bases
AACTTCTTCAAGAGTTTTGGTTCTTAAGAAATTTCATGGTATAATGGATGGTAACCGTAACGTTGCCGTCTTTTTTGTCG
GCCAATAGAAAGAGAAAAGA

Downstream 100 bases:

>100_bases
TAGAAAAGAATGACAGTATATGACTTTCTGCATTTATTACATTCCTACTTGGTATAGGAACAGCTATTATTCCTTTCTTG
CAAGGTATCAATTAGAAAAT

Product: 23S rRNA (uracil-5-)-methyltransferase RumA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 543; Mature: 543

Protein sequence:

>543_residues
MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMRVLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGI
ADLGHLSYPEQLKFKTKQVKDSLYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVATRPKVFRVDQLIEQVIKQFPEIVSVM
QNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQIAGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGL
SVAKHVKEVYGVELIPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFIKASAQTGA
DRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSKLDVDKHISVEIELDEMNLTSAESKATYAQI
KEYVWNKFELKVSTLYIAQIKKKCGIELREHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI

Sequences:

>Translated_543_residues
MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMRVLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGI
ADLGHLSYPEQLKFKTKQVKDSLYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVATRPKVFRVDQLIEQVIKQFPEIVSVM
QNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQIAGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGL
SVAKHVKEVYGVELIPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFIKASAQTGA
DRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSKLDVDKHISVEIELDEMNLTSAESKATYAQI
KEYVWNKFELKVSTLYIAQIKKKCGIELREHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI
>Mature_543_residues
MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMRVLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGI
ADLGHLSYPEQLKFKTKQVKDSLYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVATRPKVFRVDQLIEQVIKQFPEIVSVM
QNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQIAGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGL
SVAKHVKEVYGVELIPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFIKASAQTGA
DRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSKLDVDKHISVEIELDEMNLTSAESKATYAQI
KEYVWNKFELKVSTLYIAQIKKKCGIELREHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=308, Percent_Identity=33.1168831168831, Blast_Score=133, Evalue=5e-31,
Organism=Homo sapiens, GI51173878, Length=308, Percent_Identity=33.1168831168831, Blast_Score=133, Evalue=5e-31,
Organism=Homo sapiens, GI269784680, Length=421, Percent_Identity=28.2660332541568, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI269784676, Length=421, Percent_Identity=28.2660332541568, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI20149688, Length=421, Percent_Identity=28.2660332541568, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI269784678, Length=310, Percent_Identity=29.3548387096774, Blast_Score=118, Evalue=1e-26,
Organism=Escherichia coli, GI1787083, Length=372, Percent_Identity=26.6129032258064, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1789148, Length=173, Percent_Identity=31.7919075144509, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1790403, Length=207, Percent_Identity=28.5024154589372, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71997438, Length=520, Percent_Identity=22.6923076923077, Blast_Score=106, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6322909, Length=433, Percent_Identity=22.8637413394919, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24666579, Length=418, Percent_Identity=27.7511961722488, Blast_Score=142, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 61759; Mature: 61759

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS50926 TRAM ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMRVLKVNKKIGFGKVEKY
CCCCCCEEEEEEEEEECCCCCEEECCCEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHH
LVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDSLYKIAGIADVEVAETLGM
HHCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCC
EHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFFIQDPVIDQVVVALRDLLRRF
CCCCCCCCCCCCCHHHHHHHHHHCCEECCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHC
DLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVATRPKVFRVDQLIEQVIKQFPEIVSVM
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
QNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQIAGPAFYQVNTEMAEKLYQTAIDFA
HCCCCCCCCEEECCHHHHHCCCCHHHHHHCCCCEEECCCEEEEECHHHHHHHHHHHHHHH
ELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVELIPEAVENSKKNAQLNNISNAHYVCDT
HCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCEEECC
AENAMKNWLKDGIQPTVILVDPPRKGLTESFIKASAQTGADRIAYISCNVATMARDIKLY
HHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHH
QELGYELKKVQPVDLFPQTHHVETVALLSKLDVDKHISVEIELDEMNLTSAESKATYAQI
HHHCCHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCHHHHHHHHH
KEYVWNKFELKVSTLYIAQIKKKCGIELREHYNKSKKDKQIIPQCTPEKEEAIMDALRHF
HHHHCCCEEEEEHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
KMI
CCC
>Mature Secondary Structure
MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMRVLKVNKKIGFGKVEKY
CCCCCCEEEEEEEEEECCCCCEEECCCEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHH
LVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDSLYKIAGIADVEVAETLGM
HHCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCC
EHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFFIQDPVIDQVVVALRDLLRRF
CCCCCCCCCCCCCHHHHHHHHHHCCEECCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHC
DLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVATRPKVFRVDQLIEQVIKQFPEIVSVM
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
QNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQIAGPAFYQVNTEMAEKLYQTAIDFA
HCCCCCCCCEEECCHHHHHCCCCHHHHHHCCCCEEECCCEEEEECHHHHHHHHHHHHHHH
ELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVELIPEAVENSKKNAQLNNISNAHYVCDT
HCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCEEECC
AENAMKNWLKDGIQPTVILVDPPRKGLTESFIKASAQTGADRIAYISCNVATMARDIKLY
HHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHH
QELGYELKKVQPVDLFPQTHHVETVALLSKLDVDKHISVEIELDEMNLTSAESKATYAQI
HHHCCHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCHHHHHHHHH
KEYVWNKFELKVSTLYIAQIKKKCGIELREHYNKSKKDKQIIPQCTPEKEEAIMDALRHF
HHHHCCCEEEEEHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
KMI
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]