Click here to switch to the map view.

The map label for this gene is braE [H]

Identifier: 169832760

GI number: 169832760

Start: 788970

End: 789926

Strand: Direct

Name: braE [H]

Synonym: SPH_0852

Alternate gene names: 169832760

Gene position: 788970-789926 (Clockwise)

Preceding gene: 169832785

Following gene: 169834444

Centisome position: 35.13

GC content: 40.23

Gene sequence:

>957_bases
ATGAAGGAAAATTTAAAAGTTAATATTCTATGGTTACTCCTTTTGTTAGCTGGCTATAGCTTGATTAGTGTACTGGTTTC
AGTCGGAGTACTTAATCTATTCTATGTACAGATTTTACAACAAATTGGAATTAATATTATTTTGGCTGTTGGTCTCAACT
TAATCGTTGGTTTTTCAGGACAATTTTCACTTGGTCATGCTGGTTTCATGGCGATTGGTGCCTATGCAGCAGCTATTATT
GGTTCTAAATCACCAACCTACGGTGCCTTCTTTGGAGCTATGCTTGTAGGAGCTTTGCTTTCAGGAGCAGTTGCCTTACT
TGTCGGCATTCCAACCTTGCGCTTGAAGGGGGACTATCTTGCGGTAGCAACTCTAGGTGTTTCTGAAATTATCCGTATCT
TTATCATCAATGGTGGAAGCCTTACAAATGGTGCGGCAGGTATTTTAGGGATTCCTAACTTTACAACTTGGCAAATGGTT
TACTTCTTTGTCGTGATTACAACCATTGCAACCTTGAACTTCTTGCGTAGCCCAATTGGTCGTTCAACCCTCTCTGTTCG
TGAAGATGAAATCGCTGCTGAGTCAGTTGGGGTTAATACGACTAAAATTAAAATCATCGCTTTTGTCTTTGGTGCCATTA
CTGCAAGTATTGCTGGGTCACTTCAGGCAGGATTTATCGGGTCTGTTGTACCGAAAGATTACACCTTCATCAACTCAATC
AACGTTTTGATTATTGTTGTATTTGGTGGACTCGGTTCCATTACAGGTGCGATTGTTTCGGCTATTGTTCTGGGAATTTT
GAATATGCTTCTCCAAGATGTTGCTAGTGTGCGTATGATTATTTACGCTTTGGCCTTGGTATTGGTAATGATTTTCAGAC
CAGGTGGACTCCTTGGAACATGGGAACTGAGCCTATCACGTTTCTTTAAAAAATCTAAGAAGGAGGAACAAAACTAA

Upstream 100 bases:

>100_bases
AGATTTCCGTGATGCCATTGTTTATGGAATCTTGTTGTTGATCTTGATTGTCCGCCCAGCTGGTATCCTTGGTAAGAATG
TGAAAGAGAAGGTGTAAGCG

Downstream 100 bases:

>100_bases
TGGCATTACTTGAAGTAAAACAGTTAACCAAACATTTTGGTGGTCTAACAGCTGTTGGAGATGTGACTCTTGAATTGAAC
GAAGGGGAACTGGTTGGATT

Product: ABC transporter membrane-spanning permease - branched chain amino acid transport

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGINIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAII
GSKSPTYGAFFGAMLVGALLSGAVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIAGSLQAGFIGSVVPKDYTFINSI
NVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVASVRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN

Sequences:

>Translated_318_residues
MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGINIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAII
GSKSPTYGAFFGAMLVGALLSGAVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIAGSLQAGFIGSVVPKDYTFINSI
NVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVASVRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN
>Mature_318_residues
MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGINIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAII
GSKSPTYGAFFGAMLVGALLSGAVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIAGSLQAGFIGSVVPKDYTFINSI
NVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVASVRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=290, Percent_Identity=37.2413793103448, Blast_Score=161, Evalue=6e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 33565; Mature: 33565

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGINIILAVGLNLIVGFSG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEHHHHHHHHCCCCC
QFSLGHAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGALLSGAVALLVGIPTLRLKGDYL
CCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEE
AVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMVYFFVVITTIATLNFLRSPIG
EEEHHCHHHHHEEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
RSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIAGSLQAGFIGSVVPKDYTFINSI
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCC
NVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVASVRMIIYALALVLVMIFRPGGLLGT
CEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEH
WELSLSRFFKKSKKEEQN
HHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGINIILAVGLNLIVGFSG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEHHHHHHHHCCCCC
QFSLGHAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGALLSGAVALLVGIPTLRLKGDYL
CCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEE
AVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMVYFFVVITTIATLNFLRSPIG
EEEHHCHHHHHEEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
RSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIAGSLQAGFIGSVVPKDYTFINSI
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCC
NVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVASVRMIIYALALVLVMIFRPGGLLGT
CEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEH
WELSLSRFFKKSKKEEQN
HHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]