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The map label for this gene is folE [H]

Identifier: 169832677

GI number: 169832677

Start: 361031

End: 361585

Strand: Direct

Name: folE [H]

Synonym: SPH_0408

Alternate gene names: 169832677

Gene position: 361031-361585 (Clockwise)

Preceding gene: 169832986

Following gene: 169834513

Centisome position: 16.08

GC content: 43.24

Gene sequence:

>555_bases
ATGGATACACAAAAGATTGAAGCAGCTGTAAAAATGATTATCGAGGCTGTAGGAGAGAACGCTAATCGCGAGGGCTTGCA
GGAAACACCTGCTCGTGTAGCCCGTATGTATCAAGAGATTTTTTCAGGTCTTGGTCAAACAGCAGAGGAACATTTGTCAA
AATCCTTTGAAATTATTGACGATAATATGGTGGTAGAAAAGGATATCTTTTTCCATACCATGTGTGAACACCACTTCTTG
CCATTTTATGGTAGAGCGCACATTGCCTACATTCCAGATGGTCGTGTGGCAGGCTTGTCTAAGCTAGCCCGTACGGTTGA
AGTTTATTCGAAAAAACCACAAATTCAAGAACGTTTGAATATCGAAGTGGCCGATGCCTTGATGGACTATCTAGGTGCTA
AAGGAGCCTTTGTTGTCATTGAGGCGGAACATATGTGTATGAGTATGCGTGGTGTTAGAAAACCAGGCACTGCAACCTTG
ACGACAGTAGCTCGTGGTCTATTTGAAACAGATAAGGACCTCCGCGACCAAGCTTATCGTTTAATGGGGCTATAA

Upstream 100 bases:

>100_bases
ACAGATAAAAAAGAAGAGAAACAAACAGTTAGGATTGTCACAGGTTCCTTGTATTTCTTGAGCCAAGTGAGGGCCTATCT
GATGGAGAGGAAGAACGAGA

Downstream 100 bases:

>100_bases
AAAGAATCCGCTTCAAGCGGATTTTTCTAGAAAGGAATCATTATGGATCAACTGCAGATTAAGGATTTGGAAATGTTTGC
CTATCATGGTCTTTTTCCTA

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGLGQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFL
PFYGRAHIAYIPDGRVAGLSKLARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
TTVARGLFETDKDLRDQAYRLMGL

Sequences:

>Translated_184_residues
MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGLGQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFL
PFYGRAHIAYIPDGRVAGLSKLARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
TTVARGLFETDKDLRDQAYRLMGL
>Mature_184_residues
MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGLGQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFL
PFYGRAHIAYIPDGRVAGLSKLARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
TTVARGLFETDKDLRDQAYRLMGL

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=183, Percent_Identity=42.0765027322404, Blast_Score=152, Evalue=2e-37,
Organism=Homo sapiens, GI4503949, Length=183, Percent_Identity=42.0765027322404, Blast_Score=152, Evalue=2e-37,
Organism=Homo sapiens, GI66932970, Length=143, Percent_Identity=42.6573426573427, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI66932972, Length=143, Percent_Identity=42.6573426573427, Blast_Score=119, Evalue=1e-27,
Organism=Escherichia coli, GI1788476, Length=159, Percent_Identity=40.251572327044, Blast_Score=112, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17560486, Length=176, Percent_Identity=43.1818181818182, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6321706, Length=182, Percent_Identity=42.8571428571429, Blast_Score=152, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24656782, Length=173, Percent_Identity=41.0404624277457, Blast_Score=134, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24656796, Length=174, Percent_Identity=40.8045977011494, Blast_Score=134, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24656791, Length=174, Percent_Identity=40.8045977011494, Blast_Score=134, Evalue=5e-32,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 20713; Mature: 20713

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1 ; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGLGQTAEEHLSKSFEIID
CCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHC
DNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKLARTVEVYSKKPQIQERLN
CCEEEHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHC
IEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATLTTVARGLFETDKDLRDQAYR
HHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHH
LMGL
HHCC
>Mature Secondary Structure
MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGLGQTAEEHLSKSFEIID
CCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHC
DNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKLARTVEVYSKKPQIQERLN
CCEEEHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHC
IEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATLTTVARGLFETDKDLRDQAYR
HHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHH
LMGL
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA