Click here to switch to the map view.

The map label for this gene is ruvA [H]

Identifier: 169832637

GI number: 169832637

Start: 249025

End: 249618

Strand: Direct

Name: ruvA [H]

Synonym: SPH_0282

Alternate gene names: 169832637

Gene position: 249025-249618 (Clockwise)

Preceding gene: 169833770

Following gene: 169832655

Centisome position: 11.09

GC content: 43.6

Gene sequence:

>594_bases
ATGTACGCATATTTAAAAGGAATCATTACCAAAATTACTGCCAAATACATTGTTCTTGAAACCAATGGTATTGGTTATAT
CCTGCATGTGGCCAATCCTTATGCCTATTCAGGTCAGGTTAATCAGGAGGCTCAGATTTATGTGCATCAGGTTGTGCGTG
AGGACGCCCATTTGCTTTATGGATTTCGCTCAGAGGATGAGAAAAAGCTCTTTCTTAGTCTGATTTCGGTCTCTGGGATT
GGTCCTGTATCAGCTCTTGCTATTATCGCTGCTGATGACAATGCTGGCTTGGTTCAAGCCATTGAAACCAAGAACATCAC
CTACTTGACCAAGTTCCCTAAAATTGGCAAGAAAACAGCCCAGCAGATGGTGCTGGACTTGGAAGGCAAGGTAGTAGTTG
CAGGAGATGACCTTCCTGCCAAGGTCGCAGTGCAAGCAAGTGCTGAAAACCAAGAATTGGAAGAAGCTATGGAAGCCATG
TTGGCTCTGGGCTACAAGGCAACAGAGCTCAAGAAAATCAAGAAATTCTTTGAAGGAACGACAGATACAGCTGAGAACTA
TATCAAGTCGGCCCTTAAAATGTTGGTCAAATAG

Upstream 100 bases:

>100_bases
GATTGTTTTTGATATACTGTATTTGTGATAATCGATTCTCGCTCCTCGGATAAAGAAAATATGATATACTAGATAAACGA
AATAAGAGAGAAGGAACACT

Downstream 100 bases:

>100_bases
GAGCAGAGAATGACAAAACGTTGTTCGTGGGTCAAGATGACCAACCCACTCTACATCGCCTATCATGATGAGGAGTGGGG
CCAGCCCCTCCATGATGACC

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEAQIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGI
GPVSALAIIAADDNAGLVQAIETKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAM
LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK

Sequences:

>Translated_197_residues
MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEAQIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGI
GPVSALAIIAADDNAGLVQAIETKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAM
LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK
>Mature_197_residues
MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEAQIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGI
GPVSALAIIAADDNAGLVQAIETKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAM
LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family [H]

Homologues:

Organism=Escherichia coli, GI1788168, Length=201, Percent_Identity=33.8308457711443, Blast_Score=116, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994 [H]

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 21606; Mature: 21606

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEAQIYVHQVVREDAHLLY
CHHHHHHHHHHHHEEEEEEEECCCEEEEEECCCEEECCCCCCHHHHHHHHHHHHCHHEEE
GFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIETKNITYLTKFPKIGKKTA
EECCCCHHHHHHHHHHHCCCCCHHEEEEEEECCCCCEEEEEECCCEEEEECCCCHHHHHH
QQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAMLALGYKATELKKIKKFFEGT
HHHHHCCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
TDTAENYIKSALKMLVK
CHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEAQIYVHQVVREDAHLLY
CHHHHHHHHHHHHEEEEEEEECCCEEEEEECCCEEECCCCCCHHHHHHHHHHHHCHHEEE
GFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIETKNITYLTKFPKIGKKTA
EECCCCHHHHHHHHHHHCCCCCHHEEEEEEECCCCCEEEEEECCCEEEEECCCCHHHHHH
QQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAMLALGYKATELKKIKKFFEGT
HHHHHCCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
TDTAENYIKSALKMLVK
CHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA