Click here to switch to the map view.

The map label for this gene is pyk [H]

Identifier: 169832591

GI number: 169832591

Start: 929695

End: 931200

Strand: Direct

Name: pyk [H]

Synonym: SPH_1004

Alternate gene names: 169832591

Gene position: 929695-931200 (Clockwise)

Preceding gene: 169833547

Following gene: 169833769

Centisome position: 41.4

GC content: 41.1

Gene sequence:

>1506_bases
ATGAACAAACGTGTAAAAATCGTTGCAACTTTGGGTCCTGCGGTAGAAATCCGTGGTGGTAAAAAATTCGGTGAGGACGG
ATACTGGGGTGAAAAACTTGATGTTGAAGCTTCAGCTAAAAACATTGCTAAATTGATTGAAGCTGGTGCTAACACATTCC
GATTCAACTTCTCACACGGCGACCACCAAGAACAAGGTGAGCGTATGGCAACTGTTAAACTTGCGGAAAAAATTGCAGGT
AAAAAAGTTGGTTTCCTTCTTGATACAAAAGGACCTGAAATCCGTACAGAATTGTTCGAAGGTGAAGCTAAAGAATATTC
ATACAAAACTGGTGAAAAAATTCGTGTTGCAACTAAACAAGGAATCAAATCAACTCGTGAAGTGATTGCGTTGAACGTTG
CTGGTGCTCTTGATATCTATGATGATGTTGAAGTTGGTCGTCAAGTTTTGGTTGACGATGGTAAACTTGGTCTTCGTGTG
GTTGCTAAAGATGATGAAACTCGTGAATTTGAAGTTGAAGTTGAAAACGATGGTATCATCGCTAAACAAAAAGGTGTGAA
CATCCCTAACACTAAAATTCCTTTCCCAGCTCTTGCTGAACGCGATAACGACGATATCCGTTTCGGTCTTGAACAAGGTA
TCAACTTCATCGCAATTTCATTCGTACGTACTGCAAAAGATGTGAACGAAGTTCGTGCAATCTGTGAAGAAACTGGAAAC
GGACATGTTCAATTGTTCGCTAAAATCGAAAACCAACAAGGTATCGATAACTTAGACGAAATCATCGAAGCAGCTGATGG
TATTATGATTGCTCGTGGTGATATGGGTATCGAAGTACCGTTCGAAATGGTTCCAGTTTATCAAAAAATGATTATCAAGA
AAGTCAATGCTGCAGGTAAAGTTGTTATCACTGCAACAAACATGCTTGAAACAATGACTGAAAAACCACGTGCAACTCGT
TCAGAAGTATCAGACGTATTCAACGCTGTTATCGACGGAACTGACGCTACAATGCTGTCAGGCGAGTCTGCAAACGGTAA
ATACCCACTCGAGTCAGTAACTACAATGGCTACAATCGACAAGAACGCTCAAGCTCTTCTTAATGAATACGGACGTCTTG
ATTCAGATTCATTTGAGCGTAACTCTAAGACAGAAGTAATGGCTTCTGCTGTTAAAGATGCTACTAGCTCAATGGATATC
AAATTGGTTGTAACTCTTACTAAGACAGGTCATACTGCACGTTTGATTTCTAAATACCGTCCAAATGCTGACATCTTAGC
ATTGACATTTGACGAATTGACAGAACGTGGCTTGATGTTGAACTGGGGTGTTATCCCAATGTTGACAGATGCTCCATCTT
CAACTGACGATATGTTCGAAATCGCTGAACGTAAAGCGGTAGAAGCAGGTCTCGTTGAGTCAGGCGATGATATCGTTATC
GTTGCTGGTGTGCCAGTAGGAGAAGCTGTTCGCACAAACACAATGCGTATCCGCACAGTACGTTAA

Upstream 100 bases:

>100_bases
GCCGATATTGAGCTATCTAGCTTGAATAAGAGCTTGTCATAATTTACAATTTAGTTAATAAGACCAAAAAGGTCATATAT
ATAAAGGAGTCACAAAAATC

Downstream 100 bases:

>100_bases
GAAAAATATAAAAACCTATCATATCCAGCTTTCTAGCCTGTATGATGGGCTCTTTTTTTTGTCCAGGGGGCAAACCTTTC
ATAATGGATTGATACTCTTC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAKLIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAG
KKVGFLLDTKGPEIRTELFEGEAKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
VAKDDETREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIAISFVRTAKDVNEVRAICEETGN
GHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMGIEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATR
SEVSDVFNAVIDGTDATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVKDATSSMDI
KLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTDAPSSTDDMFEIAERKAVEAGLVESGDDIVI
VAGVPVGEAVRTNTMRIRTVR

Sequences:

>Translated_501_residues
MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAKLIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAG
KKVGFLLDTKGPEIRTELFEGEAKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
VAKDDETREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIAISFVRTAKDVNEVRAICEETGN
GHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMGIEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATR
SEVSDVFNAVIDGTDATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVKDATSSMDI
KLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTDAPSSTDDMFEIAERKAVEAGLVESGDDIVI
VAGVPVGEAVRTNTMRIRTVR
>Mature_501_residues
MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAKLIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAG
KKVGFLLDTKGPEIRTELFEGEAKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
VAKDDETREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIAISFVRTAKDVNEVRAICEETGN
GHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMGIEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATR
SEVSDVFNAVIDGTDATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVKDATSSMDI
KLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTDAPSSTDDMFEIAERKAVEAGLVESGDDIVI
VAGVPVGEAVRTNTMRIRTVR

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=484, Percent_Identity=38.2231404958678, Blast_Score=305, Evalue=9e-83,
Organism=Homo sapiens, GI33286422, Length=484, Percent_Identity=38.2231404958678, Blast_Score=304, Evalue=1e-82,
Organism=Homo sapiens, GI33286420, Length=484, Percent_Identity=38.2231404958678, Blast_Score=304, Evalue=1e-82,
Organism=Homo sapiens, GI10835121, Length=518, Percent_Identity=36.8725868725869, Blast_Score=300, Evalue=2e-81,
Organism=Homo sapiens, GI32967597, Length=518, Percent_Identity=36.8725868725869, Blast_Score=300, Evalue=2e-81,
Organism=Homo sapiens, GI310128732, Length=280, Percent_Identity=40.3571428571429, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI310128730, Length=280, Percent_Identity=40.3571428571429, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI310128736, Length=228, Percent_Identity=35.9649122807018, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI310128734, Length=228, Percent_Identity=35.9649122807018, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI310128738, Length=221, Percent_Identity=36.6515837104072, Blast_Score=133, Evalue=5e-31,
Organism=Escherichia coli, GI1787965, Length=501, Percent_Identity=42.1157684630739, Blast_Score=377, Evalue=1e-105,
Organism=Escherichia coli, GI1788160, Length=509, Percent_Identity=32.4165029469548, Blast_Score=214, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17544584, Length=514, Percent_Identity=36.7704280155642, Blast_Score=316, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI71984413, Length=511, Percent_Identity=38.3561643835616, Blast_Score=290, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI71984406, Length=511, Percent_Identity=38.3561643835616, Blast_Score=290, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI17506831, Length=511, Percent_Identity=38.3561643835616, Blast_Score=290, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI17506829, Length=511, Percent_Identity=38.3561643835616, Blast_Score=290, Evalue=2e-78,
Organism=Saccharomyces cerevisiae, GI6319279, Length=509, Percent_Identity=37.328094302554, Blast_Score=294, Evalue=2e-80,
Organism=Saccharomyces cerevisiae, GI6324923, Length=511, Percent_Identity=38.160469667319, Blast_Score=289, Evalue=7e-79,
Organism=Drosophila melanogaster, GI24648964, Length=514, Percent_Identity=39.8832684824903, Blast_Score=317, Evalue=9e-87,
Organism=Drosophila melanogaster, GI28571814, Length=514, Percent_Identity=39.8832684824903, Blast_Score=317, Evalue=1e-86,
Organism=Drosophila melanogaster, GI24648966, Length=413, Percent_Identity=38.498789346247, Blast_Score=270, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24581235, Length=477, Percent_Identity=31.4465408805031, Blast_Score=242, Evalue=4e-64,
Organism=Drosophila melanogaster, GI24646914, Length=263, Percent_Identity=37.6425855513308, Blast_Score=179, Evalue=5e-45,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 54813; Mature: 54813

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAKLIEAGANTFRFNFSHG
CCCEEEEEEEECCEEEECCCCCCCCCCCCCCEEECCHHHHHHHHHHHCCCCEEEEECCCC
DHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGEAKEYSYKTGEKIRVATKQ
CCHHHCCHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHCCCCHHCCCCCCCEEEEEHHH
GIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRVVAKDDETREFEVEVENDGII
HHHHHHHEEEEEECCCCCCHHHHHHCCEEEEECCCCCEEEEECCCCCEEEEEEECCCCEE
AKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIAISFVRTAKDVNEVRAICEETGN
EECCCCCCCCCCCCCCHHCCCCCCCEEEEHHHCCCEEEEEHHHHHHHHHHHHHHHHHCCC
GHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMGIEVPFEMVPVYQKMIIKKVNAAGK
CEEEEEEEECCCCCCCHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCE
VVITATNMLETMTEKPRATRSEVSDVFNAVIDGTDATMLSGESANGKYPLESVTTMATID
EEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHEEEEEC
KNAQALLNEYGRLDSDSFERNSKTEVMASAVKDATSSMDIKLVVTLTKTGHTARLISKYR
CCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHC
PNADILALTFDELTERGLMLNWGVIPMLTDAPSSTDDMFEIAERKAVEAGLVESGDDIVI
CCCCEEEEEHHHHHHCCEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEE
VAGVPVGEAVRTNTMRIRTVR
EECCCCCCHHCCCCEEEEEEC
>Mature Secondary Structure
MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAKLIEAGANTFRFNFSHG
CCCEEEEEEEECCEEEECCCCCCCCCCCCCCEEECCHHHHHHHHHHHCCCCEEEEECCCC
DHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGEAKEYSYKTGEKIRVATKQ
CCHHHCCHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHCCCCHHCCCCCCCEEEEEHHH
GIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRVVAKDDETREFEVEVENDGII
HHHHHHHEEEEEECCCCCCHHHHHHCCEEEEECCCCCEEEEECCCCCEEEEEEECCCCEE
AKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIAISFVRTAKDVNEVRAICEETGN
EECCCCCCCCCCCCCCHHCCCCCCCEEEEHHHCCCEEEEEHHHHHHHHHHHHHHHHHCCC
GHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMGIEVPFEMVPVYQKMIIKKVNAAGK
CEEEEEEEECCCCCCCHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCE
VVITATNMLETMTEKPRATRSEVSDVFNAVIDGTDATMLSGESANGKYPLESVTTMATID
EEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHEEEEEC
KNAQALLNEYGRLDSDSFERNSKTEVMASAVKDATSSMDIKLVVTLTKTGHTARLISKYR
CCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHC
PNADILALTFDELTERGLMLNWGVIPMLTDAPSSTDDMFEIAERKAVEAGLVESGDDIVI
CCCCEEEEEHHHHHHCCEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEE
VAGVPVGEAVRTNTMRIRTVR
EECCCCCCHHCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8478320; 11337471 [H]