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The map label for this gene is purA [H]

Identifier: 169832536

GI number: 169832536

Start: 21927

End: 23255

Strand: Direct

Name: purA [H]

Synonym: SPH_0025

Alternate gene names: 169832536

Gene position: 21927-23255 (Clockwise)

Preceding gene: 169834198

Following gene: 169834423

Centisome position: 0.98

GC content: 40.71

Gene sequence:

>1329_bases
ATGTATGTGAGAAAGGAAGAGCCTGAGGGCTCAGACAAAATTATGACTTCAGTTGTTGTTGTAGGTACCCAATGGGGTGA
TGAAGGTAAAGGGAAGATTACAGACTTCCTTTCAGCGAATGCAGAAGTGATTGCACGTTACCAAGGTGGTGATAATGCTG
GTCACACGATTGTGATTGACGGTAAGAAATTTAAGTTGCACTTGATTCCATCTGGGATTTTCTTCCCTGAAAAAATATCT
GTCATTGGGAATGGTATGGTTGTAAATCCTAAATCTCTTGTAAAAGAGTTGAGCTATCTTCATGAGGAAGGTGTAACAAC
TGATAACTTGCGTATTTCTGATCGTGCGCATGTTATTTTGCCTTATCATATCGAGTTGGATCGCTTGCAAGAAGAAGCTA
AGGGCGACAATAAGATTGGTACGACAATTAAGGGAATTGGTCCAGCTTATATGGACAAGGCTGCTCGTGTTGGAATTCGT
ATTGCAGATCTTTTAGATAAAGATATTTTCCGTGAGCGTTTAGAACGTAACCTTGCTGAAAAGAATCGTCTTTTTGAAAA
ATTGTATGACAGTAAAGCGATTGTTTTCGATGATATTTTTGAAGAATATTACGAATATGGTCAACAAATCAAGAAATACG
TGATAGATACATCTGTTATCTTGAATGATGCGCTTGATAATGGCAAACGTGTGCTTTTTGAAGGTGCACAAGGTGTTATG
CTAGATATCGACCAAGGTACTTATCCATTTGTTACGTCATCAAACCCTGTAGCTGGTGGTGTGACAATTGGTTCTGGTGT
CGGTCCAAGCAAGATTGACAAGGTTGTAGGTGTATGTAAAGCTTATACGAGTCGTGTAGGAGATGGTCCTTTCCCAACTG
AGTTGTTTGATGAAGTGGGAGAACGTATCCGTGAAGTGGGTCATGAATATGGTACAACAACTGGTCGTCCACGTCGTGTA
GGTTGGTTTGACTCAGTTGTGATGCGTCATAGCCGTCGTGTTTCTGGTATTACTAACCTTTCTTTGAACTCTATTGATGT
TTTGAGCGGTTTGGATACTGTGAAAATCTGTGTGGCCTATGATCTTGACGGTCAACGTATTGACTACTATCCAGCTAGTC
TTGAGCAATTGAAACGTTGCAAGCCTATCTATGAAGAGTTGCCAGGTTGGTCAGAAGATATTACCGGAGTTCGCAATTTG
GAAGATCTTCCTGAGAATGCGCGTAACTATGTTCGTCGTGTGAGTGAATTGGTTGGCGTTCGTATTTCTACTTTCTCAGT
AGGTCCTGGTCGTGAACAAACAAATATTTTAGAAAGTGTTTGGTCCTAA

Upstream 100 bases:

>100_bases
TAGTTGGAAATAGCAAATCTTTCTATTGTTTCTTCTTGATAAAAAGGCGATTTTTTATTATAATAAATTGTAAGATATAA
TTGCAGGTGAGAGTCCTGCC

Downstream 100 bases:

>100_bases
GAGATTTTTAAGATTTGTTTAAGATAGGTCGGGTATACTATAGACGGTTACAAGAAGACCTCCTAACTTGTTGTAACAAA
TATCCTAAACTTTTCTTTTT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MYVRKEEPEGSDKIMTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIVIDGKKFKLHLIPSGIFFPEKIS
VIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRAHVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIR
IADLLDKDIFRERLERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVLFEGAQGVM
LDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGPFPTELFDEVGERIREVGHEYGTTTGRPRRV
GWFDSVVMRHSRRVSGITNLSLNSIDVLSGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNL
EDLPENARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS

Sequences:

>Translated_442_residues
MYVRKEEPEGSDKIMTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIVIDGKKFKLHLIPSGIFFPEKIS
VIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRAHVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIR
IADLLDKDIFRERLERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVLFEGAQGVM
LDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGPFPTELFDEVGERIREVGHEYGTTTGRPRRV
GWFDSVVMRHSRRVSGITNLSLNSIDVLSGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNL
EDLPENARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS
>Mature_442_residues
MYVRKEEPEGSDKIMTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIVIDGKKFKLHLIPSGIFFPEKIS
VIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRAHVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIR
IADLLDKDIFRERLERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVLFEGAQGVM
LDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGPFPTELFDEVGERIREVGHEYGTTTGRPRRV
GWFDSVVMRHSRRVSGITNLSLNSIDVLSGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNL
EDLPENARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=422, Percent_Identity=45.9715639810426, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI34577063, Length=423, Percent_Identity=42.5531914893617, Blast_Score=336, Evalue=3e-92,
Organism=Homo sapiens, GI40316944, Length=392, Percent_Identity=44.8979591836735, Blast_Score=326, Evalue=3e-89,
Organism=Escherichia coli, GI1790620, Length=423, Percent_Identity=48.2269503546099, Blast_Score=416, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI25146553, Length=425, Percent_Identity=43.5294117647059, Blast_Score=341, Evalue=4e-94,
Organism=Caenorhabditis elegans, GI25146555, Length=425, Percent_Identity=43.5294117647059, Blast_Score=340, Evalue=6e-94,
Organism=Saccharomyces cerevisiae, GI6324109, Length=424, Percent_Identity=42.2169811320755, Blast_Score=357, Evalue=2e-99,
Organism=Drosophila melanogaster, GI21356033, Length=419, Percent_Identity=43.6754176610979, Blast_Score=358, Evalue=4e-99,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 49233; Mature: 49233

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYVRKEEPEGSDKIMTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIVID
CCCCCCCCCCHHHHEEEEEEEEECCCCCCCCCHHHHHCCCCEEEEEECCCCCCCCEEEEE
GKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRAHVIL
CCEEEEEEECCCCCCCCHHHEECCCEEECHHHHHHHHHHHHHCCCCCCCEEECCCEEEEE
PYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERLERNLAE
EEECCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
KNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVLFEGAQGVM
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEE
LDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGPFPTELFDEVG
EEECCCCCCEEECCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
ERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVLSGLDTVKICVAY
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCEEEEEEEE
DLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPENARNYVRRVSELVGV
CCCCCEEECCCHHHHHHHHCCHHHHHCCCCCHHHCCHHHHHHCHHHHHHHHHHHHHHHCC
RISTFSVGPGREQTNILESVWS
EEEEEECCCCCHHHHHHHHHCC
>Mature Secondary Structure
MYVRKEEPEGSDKIMTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIVID
CCCCCCCCCCHHHHEEEEEEEEECCCCCCCCCHHHHHCCCCEEEEEECCCCCCCCEEEEE
GKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRAHVIL
CCEEEEEEECCCCCCCCHHHEECCCEEECHHHHHHHHHHHHHCCCCCCCEEECCCEEEEE
PYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERLERNLAE
EEECCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
KNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVLFEGAQGVM
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEE
LDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGPFPTELFDEVG
EEECCCCCCEEECCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
ERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVLSGLDTVKICVAY
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCEEEEEEEE
DLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPENARNYVRRVSELVGV
CCCCCEEECCCHHHHHHHHCCHHHHHCCCCCHHHCCHHHHHHCHHHHHHHHHHHHHHHCC
RISTFSVGPGREQTNILESVWS
EEEEEECCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA