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The map label for this gene is purB [H]

Identifier: 169832528

GI number: 169832528

Start: 137334

End: 138632

Strand: Direct

Name: purB [H]

Synonym: SPH_0163

Alternate gene names: 169832528

Gene position: 137334-138632 (Clockwise)

Preceding gene: 169832671

Following gene: 169833244

Centisome position: 6.12

GC content: 46.57

Gene sequence:

>1299_bases
ATGATCAACCGTTACTCTCGCCCTGAGATGGCGAATATTTGGAGTGAAGAAAATAAATACCGTGCTTGGCTTGAGGTGGA
AATCCTCTCTGACGAGGCATGGGCTGAGTTGGGGGAAATCCCTAAGGAAGATGTGGCTTTGATTCGCAAGAAGGCGGACT
TTGACATCGACCGTATTTTGGAAATTGAGCAGGAGACGCGCCACGATGTGGTGGCTTTCACGCGTGCGGTTTCTGAGACT
CTTGGTGAAGAGCGCAAGTGGGTTCACTATGGGTTAACTTCTACCGACGTGGTGGATACTGCTTATGGTTACCTCTACAA
GCAGGCCAACGACATCATCCGTCGTGACCTTGAAAACTTCACTAATATCATCGCTGACAAGGCCAAGGAGCACAAGTTCA
CCATCATGATGGGGCGTACTCATGGTGTGCACGCTGAGCCGACAACCTTTGGTCTTAAATTGGCAACTTGGTACAGCGAA
ATGAAACGCAATATCGAGCGCTTCGAGCATGCGGCTGCTGGTGTAGAAGCTGGTAAGATTTCTGGTGCGGTTGGGAACTT
TGCCAATATCCCACCATTTGTAGAGGAGTATGTCTGCGATAAACTTGGCATCCGTGCCCAAGAAATCTCTACACAAGTCC
TTCCTCGTGACCTTCACGCTGAGTACTTTGCGGTTCTTGCCAGCATTGCGACTTCAATAGAACGTATGGCGACTGAGATT
CGTGGTCTACAAAAATCTGAGCAACGCGAAGTAGAAGAGTTCTTTGCTAAAGGGCAAAAAGGGTCTTCAGCAATGCCTCA
CAAACGCAACCCAATCGGTTCTGAAAATATGACTGGTCTGGCGCGTGTCATTCGTGGTCACATGATTACGGCTTATGAAA
ACGTCGCTCTCTGGCACGAACGCGATATTTCTCACTCATCAGCTGAGCGTATCATCACACCAGATACGACCATTTTGATT
GACTACATGCTCAACCGTTTTGGAAATATCGTCAAGAACTTGACAGTCTTCCCAGAAAATATGATCCGAAACATGAACTC
GACTTTTGGTCTTATCTTTAGCCAACGGGCTATGTTGACATTGATTGAAAAAGGCATGACCCGTGAGCAAGCCTATGACT
TGGTGCAACCAAAAACAGCCTACTCTTGGGACAACCAAGTAGACTTTAAACCACTTCTTGAGGCAGATTCAGAAGTAACA
TCACGTCTCACTCAAGAAGAAATCGATGAAATCTTCAACCCAGTTTATTACACCAAACGAGTGGATGATATCTTTGAACG
TCTTGGACTAGGTGATTAA

Upstream 100 bases:

>100_bases
ACCCGTCTGTGTTTATTGGAAAAGAGGAGTTTGAGTAGAAATCTTGGTTTACCTAGATAGCTTATTCCCAACAGCTTAAG
AAGAAAGGAAAAATTAACAC

Downstream 100 bases:

>100_bases
TTAAAAAATAAACAGCGAGCTTCAATCTCGCTGTTTATTTTTTATCGAAAAGACTTAGTCTTCTTTTCTTTTAGTGAGTC
CATAGGCTGCTAGTGTGGAC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKEDVALIRKKADFDIDRILEIEQETRHDVVAFTRAVSET
LGEERKWVHYGLTSTDVVDTAYGYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDLHAEYFAVLASIATSIERMATEI
RGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSENMTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILI
DYMLNRFGNIVKNLTVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPLLEADSEVT
SRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD

Sequences:

>Translated_432_residues
MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKEDVALIRKKADFDIDRILEIEQETRHDVVAFTRAVSET
LGEERKWVHYGLTSTDVVDTAYGYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDLHAEYFAVLASIATSIERMATEI
RGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSENMTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILI
DYMLNRFGNIVKNLTVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPLLEADSEVT
SRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD
>Mature_432_residues
MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKEDVALIRKKADFDIDRILEIEQETRHDVVAFTRAVSET
LGEERKWVHYGLTSTDVVDTAYGYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDLHAEYFAVLASIATSIERMATEI
RGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSENMTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILI
DYMLNRFGNIVKNLTVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPLLEADSEVT
SRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI183227688, Length=399, Percent_Identity=27.5689223057644, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI4557269, Length=449, Percent_Identity=25.8351893095768, Blast_Score=154, Evalue=2e-37,
Organism=Escherichia coli, GI1787376, Length=347, Percent_Identity=28.5302593659942, Blast_Score=101, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17508577, Length=447, Percent_Identity=26.1744966442953, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI32564234, Length=351, Percent_Identity=26.7806267806268, Blast_Score=92, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6323391, Length=391, Percent_Identity=28.3887468030691, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24647570, Length=443, Percent_Identity=23.9277652370203, Blast_Score=122, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49592; Mature: 49592

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKEDVALIRKKADFDIDRIL
CCCCCCCCHHHHHHCCCCCEEEEEEEEEECCHHHHHHHCCCHHHHHHHHHHCCCCHHHHH
EIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAYGYLYKQANDIIRRDLENF
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSEMKRNIERFEHAAAGVEAGKI
HHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHC
SGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDLHAEYFAVLASIATSIERMATEI
CCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
RGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSENMTGLARVIRGHMITAYENVALWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RDISHSSAERIITPDTTILIDYMLNRFGNIVKNLTVFPENMIRNMNSTFGLIFSQRAMLT
CCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHH
LIEKGMTREQAYDLVQPKTAYSWDNQVDFKPLLEADSEVTSRLTQEEIDEIFNPVYYTKR
HHHHCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHH
VDDIFERLGLGD
HHHHHHHHCCCC
>Mature Secondary Structure
MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKEDVALIRKKADFDIDRIL
CCCCCCCCHHHHHHCCCCCEEEEEEEEEECCHHHHHHHCCCHHHHHHHHHHCCCCHHHHH
EIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAYGYLYKQANDIIRRDLENF
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSEMKRNIERFEHAAAGVEAGKI
HHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHC
SGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDLHAEYFAVLASIATSIERMATEI
CCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
RGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSENMTGLARVIRGHMITAYENVALWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RDISHSSAERIITPDTTILIDYMLNRFGNIVKNLTVFPENMIRNMNSTFGLIFSQRAMLT
CCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHH
LIEKGMTREQAYDLVQPKTAYSWDNQVDFKPLLEADSEVTSRLTQEEIDEIFNPVYYTKR
HHHHCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHH
VDDIFERLGLGD
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12397186 [H]