Click here to switch to the map view.

The map label for this gene is dnaA

Identifier: 169832473

GI number: 169832473

Start: 197

End: 1558

Strand: Direct

Name: dnaA

Synonym: SPH_0001

Alternate gene names: 169832473

Gene position: 197-1558 (Clockwise)

Preceding gene: NA

Following gene: 169834446

Centisome position: 0.01

GC content: 36.34

Gene sequence:

>1362_bases
TTGAAAGAAAAACAATTTTGGAATCGTATATTAGAATTTGCACAAGAAAGACTGACTCGATCCATGTATGATTTCTATGC
TATTCAAGCTGAACTCATCAAGGTAGAGGAAAATGTTGCCACTATATTTCTACCTCGCTCTGAAATGGAAATGGTCTGGG
AAAAACAACTAAAAGATATTATTGTAGTAGCTGGTTTTGAAATTTATGACGCTGAAATAACTCCCCACTATATTTTCACC
AAACCTCAAGATACGACTAGCTCACAAGTTGAAGAAGCTACAAATTTAACTCTTTATGACTATAGTCCAAAGTTAGTATC
TATTCCTTATTCAGATACGGGATTAAAAGAAAAGTATACCTTTGATAATTTTATTCAAGGGGATGGAAATGTTTGGGCTG
TATCAGCCGCTTTAGCTGTCTCTGAAGATTTGGCTCTGACCTATAACCCTCTTTTTATCTATGGAGGACCAGGCCTTGGT
AAGACTCACTTATTAAACGCTATTGGAAATGAAATTCTAAAAAATATTCCTAATGCGCGTGTTAAATATATCCCTGCCGA
AAGCTTTATTAATGACTTTCTTGATCACCTAAGACTTGGGGAAATGGAAAAGTTTAAAAAGACCTATCGTAGTCTTGATC
TTTTGTTAATCGATGATATACAGTCACTCAGCGGAAAAAAAGTCGCAACTCAGGAAGAATTTTTCAATACCTTTAACGCC
CTTCATGACAAGCAAAAACAGATTGTCCTAACGAGTGATCGTAGTCCAAAACATCTAGAAGGGCTCGAGGAGAGGCTTGT
CACGCGTTTTAGTTGGGGATTGACACAAACTATCACACCCCCTGACTTTGAAACACGTATTGCCATTTTACAAAGTAAGA
CGGAACATTTAGGCTACAATTTCCAAAGTGATACTCTAGAATACCTAGCTGGGCAATTTGATTCAAATGTTCGAGATCTT
GAGGGAGCCATCAACGACATCACTTTAATTGCCAGAGTAAAAAAAATCAAGGATATCACTATTGATATTGCTGCAGAAGC
CATTAGAGCCCGCAAACAAGATGTTAGCCAAATGCTCGTCATCCCAATTGATAAAATCCAAACTGAAGTTGGTAACTTTT
ATGGTGTTAGTATCAAAGAAATGAAGGGAAGTAGACGCCTTCAAAATATTGTTTTGGCCCGTCAAGTAGCCATGTATTTA
TCTAGAGAACTAACAGATAATAGTCTTCCAAAAATTGGGAAGGAATTTGGGGGAAAAGATCATACCACAGTCATTCATGC
CCATGCCAAAATAAAATCTTTGATTGATCAAGACGATAATTTACGTTTAGAAATTGAATCAATCAAAAAGAAAATCAAAT
AA

Upstream 100 bases:

>100_bases
AACATTGTGGATTATTTTTCACAGCTTGTGGAAAATTCTTGCTATCTATGGTAAAATATCTCTAGTATTAAACTTTTAAA
TAGTAAAGGAGGAGAAAGGA

Downstream 100 bases:

>100_bases
TTTGTGGATAACTTTTAGTTTTTTATCTTTTTTATCCACATTTTTTAAACAAGCTAAAAAACTTGATATGACTTGTTTAA
AGGCTGTTTTCCACAGATTT

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFLPRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFT
KPQDTTSSQVEEATNLTLYDYSPKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLIDDIQSLSGKKVATQEEFFNTFNA
LHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQTITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDL
EGAINDITLIARVKKIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVLARQVAMYL
SRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKKKIK

Sequences:

>Translated_453_residues
MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFLPRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFT
KPQDTTSSQVEEATNLTLYDYSPKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLIDDIQSLSGKKVATQEEFFNTFNA
LHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQTITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDL
EGAINDITLIARVKKIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVLARQVAMYL
SRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKKKIK
>Mature_453_residues
MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFLPRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFT
KPQDTTSSQVEEATNLTLYDYSPKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLIDDIQSLSGKKVATQEEFFNTFNA
LHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQTITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDL
EGAINDITLIARVKKIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVLARQVAMYL
SRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKKKIK

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family

Homologues:

Organism=Escherichia coli, GI2367267, Length=465, Percent_Identity=37.4193548387097, Blast_Score=296, Evalue=2e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNAA_STRP2 (Q04N63)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_815417.1
- ProteinModelPortal:   Q04N63
- SMR:   Q04N63
- STRING:   Q04N63
- EnsemblBacteria:   EBSTRT00000018868
- GeneID:   4442762
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_0001
- eggNOG:   COG0593
- GeneTree:   EBGT00050000028345
- HOGENOM:   HBG695666
- OMA:   CHTLNAL
- ProtClustDB:   PRK00149
- GO:   GO:0005737
- HAMAP:   MF_00377
- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921
- Gene3D:   G3DSA:1.10.1750.10
- PRINTS:   PR00051
- SMART:   SM00382
- SMART:   SM00760
- TIGRFAMs:   TIGR00362

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C; SSF48295 Trp_repress_rep

EC number: NA

Molecular weight: Translated: 51783; Mature: 51783

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFLPRSEMEMVWEKQLKDI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEEEEECCHHHHHHHHHHHHHE
IVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYSPKLVSIPYSDTGLKEKYT
EEEECCEEEECCCCCEEEEECCCCCCHHHHHHHHCEEEEECCCCEEECCCCCCCCCCCCC
FDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLGKTHLLNAIGNEILKNIPNAR
CCCEECCCCCEEEEEEHHHHCCCCEEEECCEEEECCCCCCHHHHHHHHHHHHHHHCCCCC
VKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLIDDIQSLSGKKVATQEEFFNTFNA
EEEECHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEECHHHCCCCCCCHHHHHHHHHHH
LHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQTITPPDFETRIAILQSKTEHLGYN
HHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
FQSDTLEYLAGQFDSNVRDLEGAINDITLIARVKKIKDITIDIAAEAIRARKQDVSQMLV
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
IPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVLARQVAMYLSRELTDNSLPKIGKEFGGKD
EEHHHHHHHHCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCC
HTTVIHAHAKIKSLIDQDDNLRLEIESIKKKIK
CCEEEEHHHHHHHHHCCCCCCEEEHHHHHHHCC
>Mature Secondary Structure
MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFLPRSEMEMVWEKQLKDI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEEEEECCHHHHHHHHHHHHHE
IVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYSPKLVSIPYSDTGLKEKYT
EEEECCEEEECCCCCEEEEECCCCCCHHHHHHHHCEEEEECCCCEEECCCCCCCCCCCCC
FDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLGKTHLLNAIGNEILKNIPNAR
CCCEECCCCCEEEEEEHHHHCCCCEEEECCEEEECCCCCCHHHHHHHHHHHHHHHCCCCC
VKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLIDDIQSLSGKKVATQEEFFNTFNA
EEEECHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEECHHHCCCCCCCHHHHHHHHHHH
LHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQTITPPDFETRIAILQSKTEHLGYN
HHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
FQSDTLEYLAGQFDSNVRDLEGAINDITLIARVKKIKDITIDIAAEAIRARKQDVSQMLV
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
IPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVLARQVAMYLSRELTDNSLPKIGKEFGGKD
EEHHHHHHHHCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCC
HTTVIHAHAKIKSLIDQDDNLRLEIESIKKKIK
CCEEEEHHHHHHHHHCCCCCCEEEHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA