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The map label for this gene is bbmA [H]

Identifier: 169832387

GI number: 169832387

Start: 1055336

End: 1057075

Strand: Direct

Name: bbmA [H]

Synonym: SPH_1147

Alternate gene names: 169832387

Gene position: 1055336-1057075 (Clockwise)

Preceding gene: 169833007

Following gene: 169832886

Centisome position: 47.0

GC content: 39.25

Gene sequence:

>1740_bases
ATGGAATTGAGTGCTATTTACCATAGGCCTGAGTCGGAGTATGCCTATCTTTATAAGGATAAGAAACTCCATATTCGAAT
TCGAACTAAGAAAGGGGACATTGAAAGCATTAACTTGCACTATGGGGACCCTTTTATCTTTATGGAGGAGTTTTATCAGG
ATACAAAAGAAATGGTCAAGATAACTTCTGGTACCTTATTTGACCATTGGCAGGTTGAAGTGTCAGTTGACTTTGCACAT
ATCCAGTATCTCTTTGAGATCAGAGATACAGAAGGTCAAAATATTTTGTATGGCGATAAAGGGTGTGTGGAAAATTCTCT
AGAAAATCTTCATGCAATTGGGAATGGATTTAAGTTGCCTTATCTTCATGAGATTGATGCCTGCAAGGTTCCTGACTGGG
TTTCAAATACGGTATGGTATCAGATATTTCCTGAAAGATTTGCCAATGGCAATGCTCTATTAAACCCAGAAGGGACTTTA
GACTGGGATTCATCTGTCACACCTAAGAGCGATGATTTCTTTGGTGGTGATTTACAGGGGATTATTGATCATATGGATTA
CTTGCAAGACTTGGGTATTACTGGACTATATCTTTGTCCCATCTTTGAATCTACAAGCAATCACAAGTACAATACGACAG
ATTACTTTGAAATTGACCGTCATTTTGGAGACAAGGAGACCTTTCGGGAACTGGTGGATCAAGCGCATCATCGTGGCATG
AAAGTCATGCTGGATGCGGTATTTAATCATATTGGTTCGCAATCTCTTCAATGGAAAAATGTCGTCAAAAATGGTGAACA
GTCTGCTTATAAGGATTGGTTCCATATTCAACAATTCCCAGTGACAACTGAAAAGCTAGTTAATAAGAGAGACTTACCCT
ATCATGTTTTTGGTTTCGAGGACTATATGCCTAAGCTAAATACAGCCAATCCAGAGGTCAAGAATTATCTTTTAAAGGTT
GCGACTTATTGGATTGAAGAGTTTAATATCGATGCTTGGCGTTTGGATGTGGCTAATGAGATTGACCATCAGTTCTGGAA
GGATTTTCGTAAGGCAGTTTTAGCTAAAAATCCTGATCTTTATATCCTAGGAGAAGTCTGGCATACATCTCAGCCTTGGC
TAAATGGAGATGAGTTCCATGCCGTCATGAATTATCCTTTATCTGATAGTATCAAGGACTATTTCTTACGAGGAATTAAG
AAGACAGACCAGTTCATCGATGAAATCAATGGAGAGTCTATGTATTACAAGCAGCAGATTTCAGAGGTCATGTTTAATCT
CTTGGATTCACATGATACAGAGCGAATCCTGTGGACGGCCAATGAAGATGTTCAACTGGTTAAATCAGCCTTAGCCTTTC
TCTTTTTACAAAAAGGAACACCGTGCATTTATTACGGAACCGAGCTAGCCTTGACTGGAGGACCAGATCCAGATTGTCGT
CGTTGTATGCCTTGGGAACGTGTATCAAGTGACAATGATATGCTGAACTTTATGAAGAGGCTGATTAAGATTCGGAAATA
CGCGTCAGTAATCATTTCGCATGGCAAGTATAGCCTTCAAGAAATCAAATCTGATCTAGTAGCTCTGGAATGGAAATACG
AAGGACGGATCCTCAAAGCAATATTCAACCAATCAACAGAAGATTATCTTTTAGAGAAAGAAGCAGTAGCACTAGCAAGC
AATTGCCAAGAATTGGAGAATCAGCTTGTCATCTCTCCAGATGGATTTGTGATTTTCTAA

Upstream 100 bases:

>100_bases
GGACATTCCTTGTAAATAGTTTACAAAAATAGTATACTGGATTTATTGAGTTTGAAAACGTTTGCGTAAAATTTGAATGA
ATACTTTAGGAGACAAATTG

Downstream 100 bases:

>100_bases
AAACTAGTTGATGAAGATTATGGTACATTTCATATCTTATATAGTATAATAAGGCTAGTTACTAAACTTGTAAAGGAGAA
CTTAAATGAATTGTAGAGGA

Product: neopullulanase

Products: alpha-D-glucose; beta-D-glucose; amylose(n-1) [C]

Alternate protein names: NA

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MELSAIYHRPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDPFIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFAH
IQYLFEIRDTEGQNILYGDKGCVENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFANGNALLNPEGTL
DWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEIDRHFGDKETFRELVDQAHHRGM
KVMLDAVFNHIGSQSLQWKNVVKNGEQSAYKDWFHIQQFPVTTEKLVNKRDLPYHVFGFEDYMPKLNTANPEVKNYLLKV
ATYWIEEFNIDAWRLDVANEIDHQFWKDFRKAVLAKNPDLYILGEVWHTSQPWLNGDEFHAVMNYPLSDSIKDYFLRGIK
KTDQFIDEINGESMYYKQQISEVMFNLLDSHDTERILWTANEDVQLVKSALAFLFLQKGTPCIYYGTELALTGGPDPDCR
RCMPWERVSSDNDMLNFMKRLIKIRKYASVIISHGKYSLQEIKSDLVALEWKYEGRILKAIFNQSTEDYLLEKEAVALAS
NCQELENQLVISPDGFVIF

Sequences:

>Translated_579_residues
MELSAIYHRPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDPFIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFAH
IQYLFEIRDTEGQNILYGDKGCVENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFANGNALLNPEGTL
DWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEIDRHFGDKETFRELVDQAHHRGM
KVMLDAVFNHIGSQSLQWKNVVKNGEQSAYKDWFHIQQFPVTTEKLVNKRDLPYHVFGFEDYMPKLNTANPEVKNYLLKV
ATYWIEEFNIDAWRLDVANEIDHQFWKDFRKAVLAKNPDLYILGEVWHTSQPWLNGDEFHAVMNYPLSDSIKDYFLRGIK
KTDQFIDEINGESMYYKQQISEVMFNLLDSHDTERILWTANEDVQLVKSALAFLFLQKGTPCIYYGTELALTGGPDPDCR
RCMPWERVSSDNDMLNFMKRLIKIRKYASVIISHGKYSLQEIKSDLVALEWKYEGRILKAIFNQSTEDYLLEKEAVALAS
NCQELENQLVISPDGFVIF
>Mature_579_residues
MELSAIYHRPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDPFIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFAH
IQYLFEIRDTEGQNILYGDKGCVENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFANGNALLNPEGTL
DWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEIDRHFGDKETFRELVDQAHHRGM
KVMLDAVFNHIGSQSLQWKNVVKNGEQSAYKDWFHIQQFPVTTEKLVNKRDLPYHVFGFEDYMPKLNTANPEVKNYLLKV
ATYWIEEFNIDAWRLDVANEIDHQFWKDFRKAVLAKNPDLYILGEVWHTSQPWLNGDEFHAVMNYPLSDSIKDYFLRGIK
KTDQFIDEINGESMYYKQQISEVMFNLLDSHDTERILWTANEDVQLVKSALAFLFLQKGTPCIYYGTELALTGGPDPDCR
RCMPWERVSSDNDMLNFMKRLIKIRKYASVIISHGKYSLQEIKSDLVALEWKYEGRILKAIFNQSTEDYLLEKEAVALAS
NCQELENQLVISPDGFVIF

Specific function: Hydrolyzes beta-cyclodextrin to maltose and glucose, soluble starch to maltose and glucose, and pullulan to panose with trace amounts of maltose and glucose. It is also able to hydrolyze acarbose. Can also exhibit a transglycosylation activity transferrin

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family. BbmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=407, Percent_Identity=22.3587223587224, Blast_Score=71, Evalue=4e-12,
Organism=Escherichia coli, GI1786604, Length=488, Percent_Identity=29.0983606557377, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1790687, Length=414, Percent_Identity=26.5700483091787, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1789995, Length=200, Percent_Identity=29.5, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321726, Length=287, Percent_Identity=22.9965156794425, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322245, Length=229, Percent_Identity=23.5807860262009, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319776, Length=145, Percent_Identity=27.5862068965517, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6321731, Length=145, Percent_Identity=27.5862068965517, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324416, Length=289, Percent_Identity=22.4913494809689, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6322241, Length=289, Percent_Identity=22.4913494809689, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6322021, Length=289, Percent_Identity=22.4913494809689, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24586587, Length=230, Percent_Identity=27.3913043478261, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI221330053, Length=243, Percent_Identity=27.9835390946502, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24586599, Length=241, Percent_Identity=27.8008298755187, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24583747, Length=229, Percent_Identity=28.82096069869, Blast_Score=84, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24583749, Length=229, Percent_Identity=28.82096069869, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24586597, Length=225, Percent_Identity=26.6666666666667, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24586589, Length=263, Percent_Identity=26.9961977186312, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24583745, Length=224, Percent_Identity=27.6785714285714, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24586591, Length=225, Percent_Identity=25.7777777777778, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI45549022, Length=226, Percent_Identity=25.6637168141593, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24586593, Length=223, Percent_Identity=26.0089686098655, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: 3.2.1.20 [C]

Molecular weight: Translated: 67639; Mature: 67639

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELSAIYHRPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDPFIFMEEFYQDTKEMVK
CCCCHHEECCCCCEEEEEECCEEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
ITSGTLFDHWQVEVSVDFAHIQYLFEIRDTEGQNILYGDKGCVENSLENLHAIGNGFKLP
HHCCCEEEEEEEEEEEEHHHEEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHCCCCCCC
YLHEIDACKVPDWVSNTVWYQIFPERFANGNALLNPEGTLDWDSSVTPKSDDFFGGDLQG
EEECCCCCCCCCHHCCCEEEEECHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHH
IIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEIDRHFGDKETFRELVDQAHHRGM
HHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCEEEEHHHCCCHHHHHHHHHHHHHCCH
KVMLDAVFNHIGSQSLQWKNVVKNGEQSAYKDWFHIQQFPVTTEKLVNKRDLPYHVFGFE
HHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEECHH
DYMPKLNTANPEVKNYLLKVATYWIEEFNIDAWRLDVANEIDHQFWKDFRKAVLAKNPDL
HHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCCE
YILGEVWHTSQPWLNGDEFHAVMNYPLSDSIKDYFLRGIKKTDQFIDEINGESMYYKQQI
EEEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
SEVMFNLLDSHDTERILWTANEDVQLVKSALAFLFLQKGTPCIYYGTELALTGGPDPDCR
HHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEECCEEEECCCCCCCHH
RCMPWERVSSDNDMLNFMKRLIKIRKYASVIISHGKYSLQEIKSDLVALEWKYEGRILKA
HCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEEEEECCCHHHH
IFNQSTEDYLLEKEAVALASNCQELENQLVISPDGFVIF
HHCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEC
>Mature Secondary Structure
MELSAIYHRPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDPFIFMEEFYQDTKEMVK
CCCCHHEECCCCCEEEEEECCEEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
ITSGTLFDHWQVEVSVDFAHIQYLFEIRDTEGQNILYGDKGCVENSLENLHAIGNGFKLP
HHCCCEEEEEEEEEEEEHHHEEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHCCCCCCC
YLHEIDACKVPDWVSNTVWYQIFPERFANGNALLNPEGTLDWDSSVTPKSDDFFGGDLQG
EEECCCCCCCCCHHCCCEEEEECHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHH
IIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEIDRHFGDKETFRELVDQAHHRGM
HHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCEEEEHHHCCCHHHHHHHHHHHHHCCH
KVMLDAVFNHIGSQSLQWKNVVKNGEQSAYKDWFHIQQFPVTTEKLVNKRDLPYHVFGFE
HHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEECHH
DYMPKLNTANPEVKNYLLKVATYWIEEFNIDAWRLDVANEIDHQFWKDFRKAVLAKNPDL
HHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCCE
YILGEVWHTSQPWLNGDEFHAVMNYPLSDSIKDYFLRGIKKTDQFIDEINGESMYYKQQI
EEEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
SEVMFNLLDSHDTERILWTANEDVQLVKSALAFLFLQKGTPCIYYGTELALTGGPDPDCR
HHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEECCEEEECCCCCCCHH
RCMPWERVSSDNDMLNFMKRLIKIRKYASVIISHGKYSLQEIKSDLVALEWKYEGRILKA
HCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEEEEECCCHHHH
IFNQSTEDYLLEKEAVALASNCQELENQLVISPDGFVIF
HHCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): {} [C]

Substrates: Maltose; H2O; 1,4-alpha-D-glucan [C]

Specific reaction: Maltose + H2O = 2 alpha-D-glucose H2O + 1,4-alpha-D-glucan = beta-D-glucose + amylose(n-1) [C]

General reaction: O-Glycosyl bond hydrolysis; Exohydrolysis [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]