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The map label for this gene is appA [H]

Identifier: 169825255

GI number: 169825255

Start: 1728524

End: 1730128

Strand: Direct

Name: appA [H]

Synonym: FMG_1558

Alternate gene names: 169825255

Gene position: 1728524-1730128 (Clockwise)

Preceding gene: 169825254

Following gene: 169825256

Centisome position: 96.16

GC content: 33.58

Gene sequence:

>1605_bases
ATGAAAAAATTAAAATTACTTGCATTTTTAATGGCTTTTGCATTCATATTAACTGCATGTGGAAAGCCAGCAGACAAAAA
AGGAACCGATGCTCCTAACGCACCGGCTGTTGAAGGAACTGAAAAAGGTTCTTCTAAAAAAGGTGGACTTATGGTCCTAG
GTGTTGGATCTGATCCTTCATCTGTTAACCCTCTATTTGCAAACGACAGAGTTTCATTAACTATTACAAATGTATTGTAT
GACAAGTTATACAATGTTAAGTCAGGGAAAGTTGTATATGATGGTCTAGCTGAATCTATGACTCCATCTGAAGACCATTT
AACTTATACTTTAAAGTTAAAAGATGGTATAAAGTGGCATGATGGTAAGGACATAACAGCAGATGATATTATATTTACCT
ATGACAGTATATTAGATGATAAGCAAAATGCTAAGGGACAAAATGTTCTTAAGTCAGATGCTGGTGTTGTTAAATACAAA
AAAGTTGATGACAAGACAATTGAATACAAATTACCTAAGGTAGATATGACTTTCTTAAATGGTATAGCTGGTATCTCTCC
TATTCCGAAACATGTATTCGAAGGAGAAGCAGAAATTGCTAAGAGTCCAAAAAATGCAAAACCAATAGGAAGTGGCCCTT
TCAAATTCAAAGAACATAAGACTGGTGAATTATACCAAGTTGAAAGATTTGAAGACTACCATGGTGATGTTGCTAAACTT
GATGGTATAGCTTTCAAGGTTATTCCTGATGCAAACGCATCAATGGTTGCGCTTGAAAAAGGAGAAATTTCTGTTTCATA
TATCAAACCTAAAGACGTAGCTAAATTTGAAAAGAATGGCAACTTTGAAATTGTTACATTCCCTGAAGGAATGGTTGACA
ACCTATTCTTCAGATTAAACTCTGAAAAATTAAAGGACGTAAAAGTTCGTCAAGCTATTTCATATGCAATTGACAAAACT
AAACTAATTCAAGGAACATATTTAAGTAAAGACTTTGCAGATCCTGCATATTCAGTTTTCTCAACTAACACTGGTTTCTT
TAGTGATGATGTTGAAAAGTATGATTACAATGTAGAAAAGGCAAAAAAACTTTTAAAGGAAGCTGGTAAGGCAAACTTAG
AACTTAAATTAATGTACACTTCTGGAAGTCCTGCTCAAGAAAAAGAAAGTCTATTAATTCAAGAAATGCTTAAAAAAGTT
GGAATAAATGTAGAACTTCTTCCAATGGAAAGAGCAACTTTCATCGAAAAGTTATTAGATAAATCTAACCACGACTTTGA
CATGGCTATAAACGGATATGTAATGGGAGACAGCCCTGACTCATACGCTTCAATATTTAAGAGCGACTCTTCTGAAAACT
TCAGTAATTACAACAATCCAAAGATTGATGAGCTATTCAAACAAGCTAAAGTAGAAACTGACGAAGCTAAGCGTGGAGAA
AAATATAAAGAAATCCAACAAATACTTGCTGATGATGCAGTAGAATATCCTGTTGCAAATGTAAAATCAATTCTTGCAGT
TCGTAAAGAGTTTAAAAACTTAGATGCAGCAAACCCTGCACCTATTCACATGTTTGATTACTTCAACAAGATTGAATCAA
AATAA

Upstream 100 bases:

>100_bases
CATTAAGTTCATATTAAATAATATAATTTAGAAATTGTATTGCCCTATAGCAACAAGTATGTTAAATTATATAATGTAAA
AATACTTTTAGGAGGCAAAA

Downstream 100 bases:

>100_bases
TTAACTAATGAACAGTCCCTTTTTAGGGATTGTTCATTTATTTAGGAGATAATATGACAAGACTTATACTTAGACGACTT
TTACAAACCATACCTATGAT

Product: oligopeptide ABC transporter substrate-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 534; Mature: 534

Protein sequence:

>534_residues
MKKLKLLAFLMAFAFILTACGKPADKKGTDAPNAPAVEGTEKGSSKKGGLMVLGVGSDPSSVNPLFANDRVSLTITNVLY
DKLYNVKSGKVVYDGLAESMTPSEDHLTYTLKLKDGIKWHDGKDITADDIIFTYDSILDDKQNAKGQNVLKSDAGVVKYK
KVDDKTIEYKLPKVDMTFLNGIAGISPIPKHVFEGEAEIAKSPKNAKPIGSGPFKFKEHKTGELYQVERFEDYHGDVAKL
DGIAFKVIPDANASMVALEKGEISVSYIKPKDVAKFEKNGNFEIVTFPEGMVDNLFFRLNSEKLKDVKVRQAISYAIDKT
KLIQGTYLSKDFADPAYSVFSTNTGFFSDDVEKYDYNVEKAKKLLKEAGKANLELKLMYTSGSPAQEKESLLIQEMLKKV
GINVELLPMERATFIEKLLDKSNHDFDMAINGYVMGDSPDSYASIFKSDSSENFSNYNNPKIDELFKQAKVETDEAKRGE
KYKEIQQILADDAVEYPVANVKSILAVRKEFKNLDAANPAPIHMFDYFNKIESK

Sequences:

>Translated_534_residues
MKKLKLLAFLMAFAFILTACGKPADKKGTDAPNAPAVEGTEKGSSKKGGLMVLGVGSDPSSVNPLFANDRVSLTITNVLY
DKLYNVKSGKVVYDGLAESMTPSEDHLTYTLKLKDGIKWHDGKDITADDIIFTYDSILDDKQNAKGQNVLKSDAGVVKYK
KVDDKTIEYKLPKVDMTFLNGIAGISPIPKHVFEGEAEIAKSPKNAKPIGSGPFKFKEHKTGELYQVERFEDYHGDVAKL
DGIAFKVIPDANASMVALEKGEISVSYIKPKDVAKFEKNGNFEIVTFPEGMVDNLFFRLNSEKLKDVKVRQAISYAIDKT
KLIQGTYLSKDFADPAYSVFSTNTGFFSDDVEKYDYNVEKAKKLLKEAGKANLELKLMYTSGSPAQEKESLLIQEMLKKV
GINVELLPMERATFIEKLLDKSNHDFDMAINGYVMGDSPDSYASIFKSDSSENFSNYNNPKIDELFKQAKVETDEAKRGE
KYKEIQQILADDAVEYPVANVKSILAVRKEFKNLDAANPAPIHMFDYFNKIESK
>Mature_534_residues
MKKLKLLAFLMAFAFILTACGKPADKKGTDAPNAPAVEGTEKGSSKKGGLMVLGVGSDPSSVNPLFANDRVSLTITNVLY
DKLYNVKSGKVVYDGLAESMTPSEDHLTYTLKLKDGIKWHDGKDITADDIIFTYDSILDDKQNAKGQNVLKSDAGVVKYK
KVDDKTIEYKLPKVDMTFLNGIAGISPIPKHVFEGEAEIAKSPKNAKPIGSGPFKFKEHKTGELYQVERFEDYHGDVAKL
DGIAFKVIPDANASMVALEKGEISVSYIKPKDVAKFEKNGNFEIVTFPEGMVDNLFFRLNSEKLKDVKVRQAISYAIDKT
KLIQGTYLSKDFADPAYSVFSTNTGFFSDDVEKYDYNVEKAKKLLKEAGKANLELKLMYTSGSPAQEKESLLIQEMLKKV
GINVELLPMERATFIEKLLDKSNHDFDMAINGYVMGDSPDSYASIFKSDSSENFSNYNNPKIDELFKQAKVETDEAKRGE
KYKEIQQILADDAVEYPVANVKSILAVRKEFKNLDAANPAPIHMFDYFNKIESK

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=518, Percent_Identity=28.1853281853282, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1787052, Length=475, Percent_Identity=26.5263157894737, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1787762, Length=478, Percent_Identity=25.5230125523013, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI1787551, Length=469, Percent_Identity=24.5202558635394, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1789887, Length=468, Percent_Identity=23.9316239316239, Blast_Score=119, Evalue=7e-28,
Organism=Escherichia coli, GI87081878, Length=500, Percent_Identity=24.4, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1789397, Length=468, Percent_Identity=22.8632478632479, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1787495, Length=440, Percent_Identity=25.2272727272727, Blast_Score=100, Evalue=4e-22,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59425; Mature: 59425

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLKLLAFLMAFAFILTACGKPADKKGTDAPNAPAVEGTEKGSSKKGGLMVLGVGSDPS
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCC
SVNPLFANDRVSLTITNVLYDKLYNVKSGKVVYDGLAESMTPSEDHLTYTLKLKDGIKWH
CCCCEECCCEEEEEEHHHHHHHHHCCCCCCEEECCHHHCCCCCCCCEEEEEEECCCCEEC
DGKDITADDIIFTYDSILDDKQNAKGQNVLKSDAGVVKYKKVDDKTIEYKLPKVDMTFLN
CCCCCCCCCEEEEHHHHHCCCCCCCCCHHHHCCCCEEEEEECCCCEEEEECCCCCHHHHH
GIAGISPIPKHVFEGEAEIAKSPKNAKPIGSGPFKFKEHKTGELYQVERFEDYHGDVAKL
HHCCCCCCHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHCCCHHHC
DGIAFKVIPDANASMVALEKGEISVSYIKPKDVAKFEKNGNFEIVTFPEGMVDNLFFRLN
CCEEEEEECCCCCCEEEEECCCEEEEEECCHHHHHHHCCCCEEEEECCCCHHHHHHEEEC
SEKLKDVKVRQAISYAIDKTKLIQGTYLSKDFADPAYSVFSTNTGFFSDDVEKYDYNVEK
CHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHCCCCCCCCCCHHHHCCCHHH
AKKLLKEAGKANLELKLMYTSGSPAQEKESLLIQEMLKKVGINVELLPMERATFIEKLLD
HHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHH
KSNHDFDMAINGYVMGDSPDSYASIFKSDSSENFSNYNNPKIDELFKQAKVETDEAKRGE
CCCCCEEEEEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHCCH
KYKEIQQILADDAVEYPVANVKSILAVRKEFKNLDAANPAPIHMFDYFNKIESK
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MKKLKLLAFLMAFAFILTACGKPADKKGTDAPNAPAVEGTEKGSSKKGGLMVLGVGSDPS
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCC
SVNPLFANDRVSLTITNVLYDKLYNVKSGKVVYDGLAESMTPSEDHLTYTLKLKDGIKWH
CCCCEECCCEEEEEEHHHHHHHHHCCCCCCEEECCHHHCCCCCCCCEEEEEEECCCCEEC
DGKDITADDIIFTYDSILDDKQNAKGQNVLKSDAGVVKYKKVDDKTIEYKLPKVDMTFLN
CCCCCCCCCEEEEHHHHHCCCCCCCCCHHHHCCCCEEEEEECCCCEEEEECCCCCHHHHH
GIAGISPIPKHVFEGEAEIAKSPKNAKPIGSGPFKFKEHKTGELYQVERFEDYHGDVAKL
HHCCCCCCHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHCCCHHHC
DGIAFKVIPDANASMVALEKGEISVSYIKPKDVAKFEKNGNFEIVTFPEGMVDNLFFRLN
CCEEEEEECCCCCCEEEEECCCEEEEEECCHHHHHHHCCCCEEEEECCCCHHHHHHEEEC
SEKLKDVKVRQAISYAIDKTKLIQGTYLSKDFADPAYSVFSTNTGFFSDDVEKYDYNVEK
CHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHCCCCCCCCCCHHHHCCCHHH
AKKLLKEAGKANLELKLMYTSGSPAQEKESLLIQEMLKKVGINVELLPMERATFIEKLLD
HHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHH
KSNHDFDMAINGYVMGDSPDSYASIFKSDSSENFSNYNNPKIDELFKQAKVETDEAKRGE
CCCCCEEEEEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHCCH
KYKEIQQILADDAVEYPVANVKSILAVRKEFKNLDAANPAPIHMFDYFNKIESK
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]