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The map label for this gene is miaB

Identifier: 169824439

GI number: 169824439

Start: 805080

End: 806465

Strand: Direct

Name: miaB

Synonym: FMG_0742

Alternate gene names: 169824439

Gene position: 805080-806465 (Clockwise)

Preceding gene: 169824438

Following gene: 169824440

Centisome position: 44.79

GC content: 29.65

Gene sequence:

>1386_bases
ATGGAAGAAAATAACGATAAATTAGCCACAAATTCTTATGTTAATTTATTTATAAATAGACACGAACACGCTCCAACCTA
TTCGATAATTACTCATGGTTGTCAAATGAATGAACATGATTCTGAAAAAATAAAAACATTATTAGAAAATATGGGATTTG
AAAAATCTGATGAAAAATTAGATGCAGATTTCATAATTTTCAATACTTGCTTAGTAAGAGAAAATGCAGAGATGAAGGTT
TATGGACAACTTGGAGCATTAAAAAATTTAAAAAGAGAGAATCCAGATATGCTTATAGCTGTATGTGGCTGTATGATGCA
AACTGGTCCTGCGCGCGATATCATTAGAGAAAAGTATCCACAAGTGGATATAGTATTTGGAGTAAATAACATAAATTCAT
TACCTTATCTTATAGATAGACATCTAAGCAGTGGAAAATTAGTTGTAGATATTGAAAGAAAAGATGATATTGATGAAGAC
ATCACTATTAAAAGAGATAATGAATATGTTGGATATGTAAATATAATGACTGGATGCAATAATTTTTGTACTTATTGCAT
AGTTCCATACGCAAGAGGTAGGGAGCAAAGTAGATCTGTTGAAAGTATATTATCTGAAGTGAAAAGAATGGTAGATCAAG
GATATAAAGATATAACTCTACTAGGACAGAATGTAAATTCATACGGTAAAACACTTGAAAATCCTGTTACTTTTACAGAG
TTATTAACGAAGGTTAATGATGTTGAAGGACTTGAGAGATTAAGATTTTTAACAAGTCATCCAAAAGATATTTCTGATGA
ATTAATTGAAGCAATGGGTAAATTAGATAAAGTTTGCGAAAACATTCATTTACCATTTCAAGCAGGATCAAATTCGGTTT
TAGAAAGAATGCACAGAAGATATACAAAAGAATCTTACTTAGAAAAGGTAGAGAAATTAAAGAAAAGTGTAAAAGGAATT
ACTTTTTCAACGGATATTATAGTAGGATTTCCAGGAGAAACTGAAGAAGATTTCCAAGACACTTTAGATGTAGTTAGAAA
AGTTGGTTATGAACAAGCTTTTACATTTAAATATAATAGAAGACCTGGAACAAAGGCAGATTTGTTTGAAGATCAAGTTG
ATGAAGATGTAAAACAAGATAGACTAGAAAGACTTTTAGATGTTGCTTATCCGATTTTTTACGAGAAAAACAAATCTTAT
CTTGGAACTATACAAGAAGTATTAATTGAAGGAGAAAGTAAGAATAATCCAGAAGTCATGACTGGCAGAACTAGAACATT
CAAATTGGTAAATGTTAAATGTGATAAATCTTATATTGGTAAATTAGTAAATACGAAAATTGTAGATTTCAATTCTTTTG
CATTAACTGGGGAAATGGTGGATTAA

Upstream 100 bases:

>100_bases
ATAAATCTGATGATAACATTACAATTACATTGATTAAAATATAGAATTTATTTATAAGCTATCGAGTATCGATGGCTTAT
ATTTTATAAAGGAGATATAA

Downstream 100 bases:

>100_bases
AATGGCTTATACTCCTATGATTAAACAATATAAAGAAATAAAAGAACAAAACAAAGATTGTATATTATTTTTTAGATTAG
GAGATTTTTATGAAATGTTC

Product: 2-methylthioadenine synthetase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MEENNDKLATNSYVNLFINRHEHAPTYSIITHGCQMNEHDSEKIKTLLENMGFEKSDEKLDADFIIFNTCLVRENAEMKV
YGQLGALKNLKRENPDMLIAVCGCMMQTGPARDIIREKYPQVDIVFGVNNINSLPYLIDRHLSSGKLVVDIERKDDIDED
ITIKRDNEYVGYVNIMTGCNNFCTYCIVPYARGREQSRSVESILSEVKRMVDQGYKDITLLGQNVNSYGKTLENPVTFTE
LLTKVNDVEGLERLRFLTSHPKDISDELIEAMGKLDKVCENIHLPFQAGSNSVLERMHRRYTKESYLEKVEKLKKSVKGI
TFSTDIIVGFPGETEEDFQDTLDVVRKVGYEQAFTFKYNRRPGTKADLFEDQVDEDVKQDRLERLLDVAYPIFYEKNKSY
LGTIQEVLIEGESKNNPEVMTGRTRTFKLVNVKCDKSYIGKLVNTKIVDFNSFALTGEMVD

Sequences:

>Translated_461_residues
MEENNDKLATNSYVNLFINRHEHAPTYSIITHGCQMNEHDSEKIKTLLENMGFEKSDEKLDADFIIFNTCLVRENAEMKV
YGQLGALKNLKRENPDMLIAVCGCMMQTGPARDIIREKYPQVDIVFGVNNINSLPYLIDRHLSSGKLVVDIERKDDIDED
ITIKRDNEYVGYVNIMTGCNNFCTYCIVPYARGREQSRSVESILSEVKRMVDQGYKDITLLGQNVNSYGKTLENPVTFTE
LLTKVNDVEGLERLRFLTSHPKDISDELIEAMGKLDKVCENIHLPFQAGSNSVLERMHRRYTKESYLEKVEKLKKSVKGI
TFSTDIIVGFPGETEEDFQDTLDVVRKVGYEQAFTFKYNRRPGTKADLFEDQVDEDVKQDRLERLLDVAYPIFYEKNKSY
LGTIQEVLIEGESKNNPEVMTGRTRTFKLVNVKCDKSYIGKLVNTKIVDFNSFALTGEMVD
>Mature_461_residues
MEENNDKLATNSYVNLFINRHEHAPTYSIITHGCQMNEHDSEKIKTLLENMGFEKSDEKLDADFIIFNTCLVRENAEMKV
YGQLGALKNLKRENPDMLIAVCGCMMQTGPARDIIREKYPQVDIVFGVNNINSLPYLIDRHLSSGKLVVDIERKDDIDED
ITIKRDNEYVGYVNIMTGCNNFCTYCIVPYARGREQSRSVESILSEVKRMVDQGYKDITLLGQNVNSYGKTLENPVTFTE
LLTKVNDVEGLERLRFLTSHPKDISDELIEAMGKLDKVCENIHLPFQAGSNSVLERMHRRYTKESYLEKVEKLKKSVKGI
TFSTDIIVGFPGETEEDFQDTLDVVRKVGYEQAFTFKYNRRPGTKADLFEDQVDEDVKQDRLERLLDVAYPIFYEKNKSY
LGTIQEVLIEGESKNNPEVMTGRTRTFKLVNVKCDKSYIGKLVNTKIVDFNSFALTGEMVD

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=423, Percent_Identity=36.8794326241135, Blast_Score=278, Evalue=8e-75,
Organism=Homo sapiens, GI28872784, Length=423, Percent_Identity=36.8794326241135, Blast_Score=278, Evalue=1e-74,
Organism=Homo sapiens, GI93277076, Length=443, Percent_Identity=29.1196388261851, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI1786882, Length=441, Percent_Identity=38.0952380952381, Blast_Score=301, Evalue=6e-83,
Organism=Escherichia coli, GI1787057, Length=395, Percent_Identity=29.1139240506329, Blast_Score=165, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17553146, Length=469, Percent_Identity=36.0341151385927, Blast_Score=272, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI71996771, Length=365, Percent_Identity=24.3835616438356, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI21356207, Length=424, Percent_Identity=36.0849056603774, Blast_Score=266, Evalue=3e-71,
Organism=Drosophila melanogaster, GI19922432, Length=442, Percent_Identity=28.0542986425339, Blast_Score=161, Evalue=8e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_FINM2 (B0S1C0)

Other databases:

- EMBL:   AP008971
- RefSeq:   YP_001692050.1
- ProteinModelPortal:   B0S1C0
- SMR:   B0S1C0
- GeneID:   6020084
- GenomeReviews:   AP008971_GR
- KEGG:   fma:FMG_0742
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- ProtClustDB:   CLSK2542024
- BioCyc:   FMAG334413:FMG_0742-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 52897; Mature: 52897

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEENNDKLATNSYVNLFINRHEHAPTYSIITHGCQMNEHDSEKIKTLLENMGFEKSDEKL
CCCCCCCEECCCEEEEEEECCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
DADFIIFNTCLVRENAEMKVYGQLGALKNLKRENPDMLIAVCGCMMQTGPARDIIREKYP
CCCEEEEEEEEEECCCCEEEEEHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHCC
QVDIVFGVNNINSLPYLIDRHLSSGKLVVDIERKDDIDEDITIKRDNEYVGYVNIMTGCN
CEEEEEECCCCCCCHHHHHHCCCCCCEEEEECCCCCCCCCCEEEECCCEEEEEEEECCCC
NFCTYCIVPYARGREQSRSVESILSEVKRMVDQGYKDITLLGQNVNSYGKTLENPVTFTE
CHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCHHHHH
LLTKVNDVEGLERLRFLTSHPKDISDELIEAMGKLDKVCENIHLPFQAGSNSVLERMHRR
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
YTKESYLEKVEKLKKSVKGITFSTDIIVGFPGETEEDFQDTLDVVRKVGYEQAFTFKYNR
HHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECC
RPGTKADLFEDQVDEDVKQDRLERLLDVAYPIFYEKNKSYLGTIQEVLIEGESKNNPEVM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHCCCCCCCCCEE
TGRTRTFKLVNVKCDKSYIGKLVNTKIVDFNSFALTGEMVD
ECCCEEEEEEEEEECHHHHHHHHCCEEEECCCEEECCCCCC
>Mature Secondary Structure
MEENNDKLATNSYVNLFINRHEHAPTYSIITHGCQMNEHDSEKIKTLLENMGFEKSDEKL
CCCCCCCEECCCEEEEEEECCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
DADFIIFNTCLVRENAEMKVYGQLGALKNLKRENPDMLIAVCGCMMQTGPARDIIREKYP
CCCEEEEEEEEEECCCCEEEEEHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHCC
QVDIVFGVNNINSLPYLIDRHLSSGKLVVDIERKDDIDEDITIKRDNEYVGYVNIMTGCN
CEEEEEECCCCCCCHHHHHHCCCCCCEEEEECCCCCCCCCCEEEECCCEEEEEEEECCCC
NFCTYCIVPYARGREQSRSVESILSEVKRMVDQGYKDITLLGQNVNSYGKTLENPVTFTE
CHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCHHHHH
LLTKVNDVEGLERLRFLTSHPKDISDELIEAMGKLDKVCENIHLPFQAGSNSVLERMHRR
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
YTKESYLEKVEKLKKSVKGITFSTDIIVGFPGETEEDFQDTLDVVRKVGYEQAFTFKYNR
HHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECC
RPGTKADLFEDQVDEDVKQDRLERLLDVAYPIFYEKNKSYLGTIQEVLIEGESKNNPEVM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHCCCCCCCCCEE
TGRTRTFKLVNVKCDKSYIGKLVNTKIVDFNSFALTGEMVD
ECCCEEEEEEEEEECHHHHHHHHCCEEEECCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA