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The map label for this gene is ispG [H]

Identifier: 169824427

GI number: 169824427

Start: 790840

End: 791889

Strand: Direct

Name: ispG [H]

Synonym: FMG_0730

Alternate gene names: 169824427

Gene position: 790840-791889 (Clockwise)

Preceding gene: 169824426

Following gene: 169824428

Centisome position: 43.99

GC content: 32.48

Gene sequence:

>1050_bases
ATGAAAAGAGAAGATACAAAAAGAATTTATGTAGGAAATGTTGCCGTGGGAAATGGAGCAAGAGTTACAGTTCAATCTAT
GACTAATACTGACACAAAAGACATAGAGAGCACTGTAAAACAAATCAAAGAATTCGAATCAGTAGGTTGTGATATCTCTA
GAAGTGCAATAAATGATTTGGAAGATGCAAAAGCAATACCAGTTATAAAAAGCATGACAAATATTCCGCTTGTTGCAGAC
ATTCAATTTCATCACAAATTAGCAATAGCTGCAATTGAAAATGGATGTGACTGCATAAGAATTAATCCAGGCAATATTGG
AGGAATAGACAGGGTAAGAGAAGTTGTAGATTGTTGTAAACACCATAAAATTTCAATGAGAATAGGAGTTAACAGTGGAT
CTGTTAATCAAAAATTCATTGACAAGTATCATGGAGTAAATGTTGATTCAATATGCTACAGTTGTCTTGATCAAATAAGA
ATGATTGAGGATATGGGATTTAACGATATCAAATTATCATTAAAATCTTCTAATGTTAATATGTCAATAAAAGCCTATGA
AAAAATGTCGCAACTTTGTAATTATCCACTTCATGTTGGAATAACAGAAGCAGGTCCTTCTACTAAAGGAATTATAAAAT
CGAGTGTAGGGATAGGTAGCATTTTATCAAAAGGTATTGGAGATACTATCAGAGTATCGTTAACTGGTGATCCTAAAGAA
GAGATTATTGTAGGAAGGCAAATATTAATGAGTTTGGGATTATTAAATGAAGGGATTGAAATAATCTCATGTCCAACATG
CGCTAGAACCAAGATTGATTTAATTAGTATTGTCAATGAGGCTGAAAAAAAATTGGATAAAATAGACAAACATATCAAGG
TTGCTATTATGGGTTGTGTTGTAAATGGACCTGGCGAAGCAAAGGAAGCTGATCTTGGTATAGCTGGGGGAAATGGAGTA
GGATTAATCTTCAAAAAAGGTGAAATAATACGAAAAGTTAAGGAAGAAGAATTATTAGACGTATTAATTGAAGAAATTGA
GAGGTTATAA

Upstream 100 bases:

>100_bases
TAGGACTAATAGCTATAGTTACAATTAAAGATGTTATCAAATTATTTTAGATGTTTTAATCAGGCCTGTATAGGCCTTTT
TAAATTTGAAAGGTGAAGTA

Downstream 100 bases:

>100_bases
ATGCATTTATTACTAAACGATGTGTTGAAGAAAATAGGTCTAAAATATGATTCAAGATATCCAATCTACATCGATAAATT
AACTTACAATAAGGATAATG

Product: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MKREDTKRIYVGNVAVGNGARVTVQSMTNTDTKDIESTVKQIKEFESVGCDISRSAINDLEDAKAIPVIKSMTNIPLVAD
IQFHHKLAIAAIENGCDCIRINPGNIGGIDRVREVVDCCKHHKISMRIGVNSGSVNQKFIDKYHGVNVDSICYSCLDQIR
MIEDMGFNDIKLSLKSSNVNMSIKAYEKMSQLCNYPLHVGITEAGPSTKGIIKSSVGIGSILSKGIGDTIRVSLTGDPKE
EIIVGRQILMSLGLLNEGIEIISCPTCARTKIDLISIVNEAEKKLDKIDKHIKVAIMGCVVNGPGEAKEADLGIAGGNGV
GLIFKKGEIIRKVKEEELLDVLIEEIERL

Sequences:

>Translated_349_residues
MKREDTKRIYVGNVAVGNGARVTVQSMTNTDTKDIESTVKQIKEFESVGCDISRSAINDLEDAKAIPVIKSMTNIPLVAD
IQFHHKLAIAAIENGCDCIRINPGNIGGIDRVREVVDCCKHHKISMRIGVNSGSVNQKFIDKYHGVNVDSICYSCLDQIR
MIEDMGFNDIKLSLKSSNVNMSIKAYEKMSQLCNYPLHVGITEAGPSTKGIIKSSVGIGSILSKGIGDTIRVSLTGDPKE
EIIVGRQILMSLGLLNEGIEIISCPTCARTKIDLISIVNEAEKKLDKIDKHIKVAIMGCVVNGPGEAKEADLGIAGGNGV
GLIFKKGEIIRKVKEEELLDVLIEEIERL
>Mature_349_residues
MKREDTKRIYVGNVAVGNGARVTVQSMTNTDTKDIESTVKQIKEFESVGCDISRSAINDLEDAKAIPVIKSMTNIPLVAD
IQFHHKLAIAAIENGCDCIRINPGNIGGIDRVREVVDCCKHHKISMRIGVNSGSVNQKFIDKYHGVNVDSICYSCLDQIR
MIEDMGFNDIKLSLKSSNVNMSIKAYEKMSQLCNYPLHVGITEAGPSTKGIIKSSVGIGSILSKGIGDTIRVSLTGDPKE
EIIVGRQILMSLGLLNEGIEIISCPTCARTKIDLISIVNEAEKKLDKIDKHIKVAIMGCVVNGPGEAKEADLGIAGGNGV
GLIFKKGEIIRKVKEEELLDVLIEEIERL

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=342, Percent_Identity=44.4444444444444, Blast_Score=315, Evalue=3e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 37961; Mature: 37961

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKREDTKRIYVGNVAVGNGARVTVQSMTNTDTKDIESTVKQIKEFESVGCDISRSAINDL
CCCCCCCEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
EDAKAIPVIKSMTNIPLVADIQFHHKLAIAAIENGCDCIRINPGNIGGIDRVREVVDCCK
HHHHHCHHHHHHCCCCEEEEEEHHHHEEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHH
HHKISMRIGVNSGSVNQKFIDKYHGVNVDSICYSCLDQIRMIEDMGFNDIKLSLKSSNVN
CCEEEEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
MSIKAYEKMSQLCNYPLHVGITEAGPSTKGIIKSSVGIGSILSKGIGDTIRVSLTGDPKE
EEHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHCCCHHHHHHCCCCCEEEEEECCCCHH
EIIVGRQILMSLGLLNEGIEIISCPTCARTKIDLISIVNEAEKKLDKIDKHIKVAIMGCV
HHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEE
VNGPGEAKEADLGIAGGNGVGLIFKKGEIIRKVKEEELLDVLIEEIERL
ECCCCCCCCCCEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKREDTKRIYVGNVAVGNGARVTVQSMTNTDTKDIESTVKQIKEFESVGCDISRSAINDL
CCCCCCCEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
EDAKAIPVIKSMTNIPLVADIQFHHKLAIAAIENGCDCIRINPGNIGGIDRVREVVDCCK
HHHHHCHHHHHHCCCCEEEEEEHHHHEEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHH
HHKISMRIGVNSGSVNQKFIDKYHGVNVDSICYSCLDQIRMIEDMGFNDIKLSLKSSNVN
CCEEEEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
MSIKAYEKMSQLCNYPLHVGITEAGPSTKGIIKSSVGIGSILSKGIGDTIRVSLTGDPKE
EEHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHCCCHHHHHHCCCCCEEEEEECCCCHH
EIIVGRQILMSLGLLNEGIEIISCPTCARTKIDLISIVNEAEKKLDKIDKHIKVAIMGCV
HHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEE
VNGPGEAKEADLGIAGGNGVGLIFKKGEIIRKVKEEELLDVLIEEIERL
ECCCCCCCCCCEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842 [H]