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The map label for this gene is ansA [H]

Identifier: 169824294

GI number: 169824294

Start: 657526

End: 658524

Strand: Direct

Name: ansA [H]

Synonym: FMG_0597

Alternate gene names: 169824294

Gene position: 657526-658524 (Clockwise)

Preceding gene: 169824285

Following gene: 169824295

Centisome position: 36.58

GC content: 30.03

Gene sequence:

>999_bases
ATGAAAAAATTACTATTGATTACAACGGGAGGAACCATAGCTTCTGTTGCTACAGATAAAGGATTAGCTCCAGGTCTTCA
GGCTAAAGATATTTTAGAATATGTAAAATTTAAAAAATTTGATTTCGAATTGGATACTATTAGTTTATTTAGTATTGATT
CAACAGACATTAGACCGAAACATTGGTTACATATCAAACAAACTATAAAAGAAAATTACGATAAATATGATGGATTCGTA
ATAACACATGGAACTGATACGTTGGCATATACAGCATCCGCTTTGGGATATTTAATTCAAAAAAGTCCTAAGCCAATTGT
AATTACAGGTGCCCAAAAATCTATTTCAAATGATATTACTGATGCGAAGAATAATTTAATAGATGCATTGATATTTGCAA
GCAAAGAAAAATCACACGGTGTTAATATCGTGTTTAATGGGAAAGTTATTTGCGGGAATAGAGCCAGAAAAGTTAGAACA
AAAAGTTTCGATGCTTTTGATTCTATAAATTATCCACTAATTGCAATAATCCATGATGGAAAAGTTTTAAGATATATTGA
TGACAAATCAGATAATGAAGTAGAATTTTACGATCATTTAGATGACAGCGTGTATTTGTTGAAATTAATTCCATCGATGA
GGTCAGATATACTAGAAGATATTTTTGAAAAATATGACTGTATTATCGTAGAAAGTTATGGAGTGGGTGGAATGCCTGCA
GGAATATCTGAAGAATTTCTAAGATTAATGGATCGATACTCTACTAATAAGATAATTGTAATGACTACACAAGTTCCACA
AGAAGGATCAGATATTGGAGTATATCAAGTCGGAATGAGAATTAGAGAAAAGCTTGATGTTTTAGAATCAAAAGATATGA
CATTGGAATCAGTAGTATGCAAAATGATGTATGTTATGGGAAAGCACAATAGAGATATTCAAAAGATTCAAGATGAATTT
TATTCAATAATAAATCACGATATTTCGATAATAGAATAA

Upstream 100 bases:

>100_bases
TTATGTTTAAAGTTCATATAATGTTATTGTAAATTGTATGTAAAGTTATTTGTTAACATTTTTTAAAAATAAAGTGTTAT
ACTATTTTTAGGGGGAATGT

Downstream 100 bases:

>100_bases
TAAGATTAACGATAAAGTATTTTTTTAAATATTTTATCGTTTTTTATTTGCAAATTTTGAAAAGTTTGAGATATGAATTA
TAATTTAGTTAGAGGAAAAT

Product: L-asparaginase

Products: NA

Alternate protein names: L-ASNase 1; L-asparagine amidohydrolase 1 [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MKKLLLITTGGTIASVATDKGLAPGLQAKDILEYVKFKKFDFELDTISLFSIDSTDIRPKHWLHIKQTIKENYDKYDGFV
ITHGTDTLAYTASALGYLIQKSPKPIVITGAQKSISNDITDAKNNLIDALIFASKEKSHGVNIVFNGKVICGNRARKVRT
KSFDAFDSINYPLIAIIHDGKVLRYIDDKSDNEVEFYDHLDDSVYLLKLIPSMRSDILEDIFEKYDCIIVESYGVGGMPA
GISEEFLRLMDRYSTNKIIVMTTQVPQEGSDIGVYQVGMRIREKLDVLESKDMTLESVVCKMMYVMGKHNRDIQKIQDEF
YSIINHDISIIE

Sequences:

>Translated_332_residues
MKKLLLITTGGTIASVATDKGLAPGLQAKDILEYVKFKKFDFELDTISLFSIDSTDIRPKHWLHIKQTIKENYDKYDGFV
ITHGTDTLAYTASALGYLIQKSPKPIVITGAQKSISNDITDAKNNLIDALIFASKEKSHGVNIVFNGKVICGNRARKVRT
KSFDAFDSINYPLIAIIHDGKVLRYIDDKSDNEVEFYDHLDDSVYLLKLIPSMRSDILEDIFEKYDCIIVESYGVGGMPA
GISEEFLRLMDRYSTNKIIVMTTQVPQEGSDIGVYQVGMRIREKLDVLESKDMTLESVVCKMMYVMGKHNRDIQKIQDEF
YSIINHDISIIE
>Mature_332_residues
MKKLLLITTGGTIASVATDKGLAPGLQAKDILEYVKFKKFDFELDTISLFSIDSTDIRPKHWLHIKQTIKENYDKYDGFV
ITHGTDTLAYTASALGYLIQKSPKPIVITGAQKSISNDITDAKNNLIDALIFASKEKSHGVNIVFNGKVICGNRARKVRT
KSFDAFDSINYPLIAIIHDGKVLRYIDDKSDNEVEFYDHLDDSVYLLKLIPSMRSDILEDIFEKYDCIIVESYGVGGMPA
GISEEFLRLMDRYSTNKIIVMTTQVPQEGSDIGVYQVGMRIREKLDVLESKDMTLESVVCKMMYVMGKHNRDIQKIQDEF
YSIINHDISIIE

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=337, Percent_Identity=27.0029673590504, Blast_Score=123, Evalue=2e-28,
Organism=Escherichia coli, GI1788065, Length=315, Percent_Identity=30.7936507936508, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1789327, Length=136, Percent_Identity=37.5, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI193207372, Length=284, Percent_Identity=32.7464788732394, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17558278, Length=284, Percent_Identity=32.7464788732394, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17558280, Length=284, Percent_Identity=32.7464788732394, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI193207374, Length=284, Percent_Identity=32.7464788732394, Blast_Score=138, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6320527, Length=301, Percent_Identity=27.2425249169435, Blast_Score=86, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6323189, Length=206, Percent_Identity=30.0970873786408, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323187, Length=206, Percent_Identity=30.0970873786408, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323186, Length=206, Percent_Identity=30.0970873786408, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323184, Length=206, Percent_Identity=30.0970873786408, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24639609, Length=268, Percent_Identity=32.8358208955224, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24645530, Length=344, Percent_Identity=27.3255813953488, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 37577; Mature: 37577

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLLLITTGGTIASVATDKGLAPGLQAKDILEYVKFKKFDFELDTISLFSIDSTDIRPK
CCEEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCEEECEEEEEECCCCCCCCH
HWLHIKQTIKENYDKYDGFVITHGTDTLAYTASALGYLIQKSPKPIVITGAQKSISNDIT
HHHHHHHHHHHCCHHHCCEEEECCCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHCHHH
DAKNNLIDALIFASKEKSHGVNIVFNGKVICGNRARKVRTKSFDAFDSINYPLIAIIHDG
HHHHHHHHHHHEECCCCCCCEEEEECCEEEECCCCHHHHCCCCCHHHCCCCCEEEEEECC
KVLRYIDDKSDNEVEFYDHLDDSVYLLKLIPSMRSDILEDIFEKYDCIIVESYGVGGMPA
EEEEEECCCCCCCEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCC
GISEEFLRLMDRYSTNKIIVMTTQVPQEGSDIGVYQVGMRIREKLDVLESKDMTLESVVC
CCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
KMMYVMGKHNRDIQKIQDEFYSIINHDISIIE
HHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEC
>Mature Secondary Structure
MKKLLLITTGGTIASVATDKGLAPGLQAKDILEYVKFKKFDFELDTISLFSIDSTDIRPK
CCEEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCEEECEEEEEECCCCCCCCH
HWLHIKQTIKENYDKYDGFVITHGTDTLAYTASALGYLIQKSPKPIVITGAQKSISNDIT
HHHHHHHHHHHCCHHHCCEEEECCCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHCHHH
DAKNNLIDALIFASKEKSHGVNIVFNGKVICGNRARKVRTKSFDAFDSINYPLIAIIHDG
HHHHHHHHHHHEECCCCCCCEEEEECCEEEECCCCHHHHCCCCCHHHCCCCCEEEEEECC
KVLRYIDDKSDNEVEFYDHLDDSVYLLKLIPSMRSDILEDIFEKYDCIIVESYGVGGMPA
EEEEEECCCCCCCEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCC
GISEEFLRLMDRYSTNKIIVMTTQVPQEGSDIGVYQVGMRIREKLDVLESKDMTLESVVC
CCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
KMMYVMGKHNRDIQKIQDEFYSIINHDISIIE
HHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377; 8478318 [H]