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The map label for this gene is dfrA [H]

Identifier: 169823965

GI number: 169823965

Start: 316946

End: 317440

Strand: Direct

Name: dfrA [H]

Synonym: FMG_0268

Alternate gene names: 169823965

Gene position: 316946-317440 (Clockwise)

Preceding gene: 169823964

Following gene: 169823966

Centisome position: 17.63

GC content: 31.11

Gene sequence:

>495_bases
TTGTTAATGAAAGTTATATTAGCAGTAGACAAAAATTTTGCGATAGGAAAAGATGGAGATATGCTTTTTTCGATTCCTGA
AGACATGCAAAGATTTAAAGAATTGACTACGGGAAACATCGTAATCATGGGAAGAAAAACATTGGAATCTCTACCTGATT
CAAAACCTCTACCAAACAGAACCAACATTATAATTACAAGCAAAGATTTGGATGGAATGTTATGCGTGAAAAATGAAGAA
GAATTGCTACGCACATTAAAAGAAATAAATCCAGATCACAAAATGACAGAGTATTTGATAGGTGGCGCAAACACCATTGA
ATCATTGATTCATTTAGTTGATGAATTCGTAATTACTTATGTTGATTGTGAATTTGAATACGATGCGAAAATAAGAAACT
TATTCGAAGACCAAGATTTCTATGTATCAGAAGAATCAGACATTAAATCATACGAAGACTTGGATTATAGATATATGGTT
TTTAAAAGAAAATAG

Upstream 100 bases:

>100_bases
TACTTGTAGTAAGAATCTGCGAGGATGATTTTGATTGGACAGATGATCATGCAATGGAAAAATTGAACGAAATTGTAAGT
AATATAAGGAAGGGAGATTT

Downstream 100 bases:

>100_bases
GAAAAAGAGTATTACACATTCATGCTAAGAATATCATACGGAAAAAATTGCTTTGATTTCAAATCGAAAAAATCCGTCAA
AAAATCGTACCGCGTAAGCG

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MLMKVILAVDKNFAIGKDGDMLFSIPEDMQRFKELTTGNIVIMGRKTLESLPDSKPLPNRTNIIITSKDLDGMLCVKNEE
ELLRTLKEINPDHKMTEYLIGGANTIESLIHLVDEFVITYVDCEFEYDAKIRNLFEDQDFYVSEESDIKSYEDLDYRYMV
FKRK

Sequences:

>Translated_164_residues
MLMKVILAVDKNFAIGKDGDMLFSIPEDMQRFKELTTGNIVIMGRKTLESLPDSKPLPNRTNIIITSKDLDGMLCVKNEE
ELLRTLKEINPDHKMTEYLIGGANTIESLIHLVDEFVITYVDCEFEYDAKIRNLFEDQDFYVSEESDIKSYEDLDYRYMV
FKRK
>Mature_164_residues
MLMKVILAVDKNFAIGKDGDMLFSIPEDMQRFKELTTGNIVIMGRKTLESLPDSKPLPNRTNIIITSKDLDGMLCVKNEE
ELLRTLKEINPDHKMTEYLIGGANTIESLIHLVDEFVITYVDCEFEYDAKIRNLFEDQDFYVSEESDIKSYEDLDYRYMV
FKRK

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=162, Percent_Identity=30.2469135802469, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 19121; Mature: 19121

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMKVILAVDKNFAIGKDGDMLFSIPEDMQRFKELTTGNIVIMGRKTLESLPDSKPLPNR
CCEEEEEEECCCCEECCCCCEEEECHHHHHHHHHCCCCCEEEEECHHHHHCCCCCCCCCC
TNIIITSKDLDGMLCVKNEEELLRTLKEINPDHKMTEYLIGGANTIESLIHLVDEFVITY
CEEEEEECCCCCEEEECCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHEEE
VDCEFEYDAKIRNLFEDQDFYVSEESDIKSYEDLDYRYMVFKRK
EECCEECCHHHHHHHCCCCEEECCCCCCCHHHCCCEEEEEEECC
>Mature Secondary Structure
MLMKVILAVDKNFAIGKDGDMLFSIPEDMQRFKELTTGNIVIMGRKTLESLPDSKPLPNR
CCEEEEEEECCCCEECCCCCEEEECHHHHHHHHHCCCCCEEEEECHHHHHCCCCCCCCCC
TNIIITSKDLDGMLCVKNEEELLRTLKEINPDHKMTEYLIGGANTIESLIHLVDEFVITY
CEEEEEECCCCCEEEECCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHEEE
VDCEFEYDAKIRNLFEDQDFYVSEESDIKSYEDLDYRYMVFKRK
EECCEECCHHHHHHHCCCCEEECCCCCCCHHHCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840350; 9384377 [H]