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The map label for this gene is braB [H]

Identifier: 169823935

GI number: 169823935

Start: 280088

End: 281383

Strand: Direct

Name: braB [H]

Synonym: FMG_0238

Alternate gene names: 169823935

Gene position: 280088-281383 (Clockwise)

Preceding gene: 169823934

Following gene: 169823936

Centisome position: 15.58

GC content: 36.96

Gene sequence:

>1296_bases
TTGAATAAAAAATTTAAGGACATCATTATCGTAGGATTTGCGCTATTTGCAATATTCTTCGGTGCAGGAAATCTGATTTT
CCCGCCTTATTTGGGTGTGCTTGCAGGAACTGAATACAAAAAAGCGATGCTCGGGTTTTTGCTTACAGATCCCGTACTTC
CGATACTTGGTGTTATCATAACTGCAAAGCTCGGAGGAGCAGCAGAAGATCTCGGAAAAAGAGTTGGGAATAATTTTTCT
AAGATTCTTGGAACCGTTACCATTTTAACGATTGGACCGCTTTTTGCTGTCCCTAGGACTGCAGCTACAACTCACGAAGT
TTTTGTATCTAAAGTTTTTCCGGGTACTCCTTTGTGGGTAACATCTCTAATATTTTTCATCTTAACTGCGATTTTTGTGT
TTAATGAAACAGGAGTTATCGACAAAATCGGTAAATATTTGACTCCGGGATTGGTAATTATTTTATTAGTTATTATTGTA
AAATGCATCGTAGATCCTATTGGGGTGATGAATGTAGCAACTGAAAATCAAATATTCTTGAAAGGATTTGCGGAAGGATA
CCAAACTATGGATGCTCTTGGAGCAGCTCTCATGACAGGAATTGTGGTTTCTGATTTGAAAAGAAAAGGATACTTAAATG
AACAAGAAAGAGTGAAGCTTGTCAAGTGGGTTGGACTTGTGGCATTTTTGTTGTTGGCGATAATTTACGGCGGATTAATA
TATGTTGGAGCTACAGCTTCCAAGATTTACACAGTTCAATATACGAGAGTAGATATTTTACTTGGAACAGTGCAACATAT
TTTGGGCTCAGCTGGTAAGTTTGCGATAGGAATTGCTGTGTCACTAGCATGCTTGACGACATCCGTTGGACTTAGCGCAG
TTGCTGGAGATTTCTTATCCGGAATTACAAAAAACAAATTGGGATACAAGCCAATTGTTTTGGCGACAGTTTTTGTTTCG
GGATTAATTTCTCTTGCAGGAGTTGAAGGACTTATTAAAGCAGCAGTTCCGATTTTATCGACTGTTTATCCGATAATAAT
GGTCCTAATATTTTTAGGAATTTTCGATAAATATATTAAATACGATATGACTTTCAAAGGTGCGGTACTTGCGACTTTTG
TAGTGAGTTTGGTAGAAGTTTTGAATAGCAAATTCAAGATTTTAGAAGGATTTACAGATGTAATCAAAACTTTGCCATTA
AGTTCTGTAGGATTTGAATGGTTACTTCCTGCGATTGTAACGGGAGCGATTTTCGGATTAATCGCTTATATTCAAGATAA
AAGAAAAATTGCTTAG

Upstream 100 bases:

>100_bases
GATAATGAAATGAATAAATTTGACAATAAAACTTTTTGGAATTAAAATTTAATTATGAAATCATTTTCTATAGATATATA
GAAATACATAGGAGGATATT

Downstream 100 bases:

>100_bases
ATTAATCAGCACGAATTTTGTGCTGATTTTTTGGACTCAAATTTTACGAACAATTTTGCAATTTATTTACAAATTTTTTA
CGCTTTAATGATTGAAAAAT

Product: branched-chain amino acid transporter

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BraB [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MNKKFKDIIIVGFALFAIFFGAGNLIFPPYLGVLAGTEYKKAMLGFLLTDPVLPILGVIITAKLGGAAEDLGKRVGNNFS
KILGTVTILTIGPLFAVPRTAATTHEVFVSKVFPGTPLWVTSLIFFILTAIFVFNETGVIDKIGKYLTPGLVIILLVIIV
KCIVDPIGVMNVATENQIFLKGFAEGYQTMDALGAALMTGIVVSDLKRKGYLNEQERVKLVKWVGLVAFLLLAIIYGGLI
YVGATASKIYTVQYTRVDILLGTVQHILGSAGKFAIGIAVSLACLTTSVGLSAVAGDFLSGITKNKLGYKPIVLATVFVS
GLISLAGVEGLIKAAVPILSTVYPIIMVLIFLGIFDKYIKYDMTFKGAVLATFVVSLVEVLNSKFKILEGFTDVIKTLPL
SSVGFEWLLPAIVTGAIFGLIAYIQDKRKIA

Sequences:

>Translated_431_residues
MNKKFKDIIIVGFALFAIFFGAGNLIFPPYLGVLAGTEYKKAMLGFLLTDPVLPILGVIITAKLGGAAEDLGKRVGNNFS
KILGTVTILTIGPLFAVPRTAATTHEVFVSKVFPGTPLWVTSLIFFILTAIFVFNETGVIDKIGKYLTPGLVIILLVIIV
KCIVDPIGVMNVATENQIFLKGFAEGYQTMDALGAALMTGIVVSDLKRKGYLNEQERVKLVKWVGLVAFLLLAIIYGGLI
YVGATASKIYTVQYTRVDILLGTVQHILGSAGKFAIGIAVSLACLTTSVGLSAVAGDFLSGITKNKLGYKPIVLATVFVS
GLISLAGVEGLIKAAVPILSTVYPIIMVLIFLGIFDKYIKYDMTFKGAVLATFVVSLVEVLNSKFKILEGFTDVIKTLPL
SSVGFEWLLPAIVTGAIFGLIAYIQDKRKIA
>Mature_431_residues
MNKKFKDIIIVGFALFAIFFGAGNLIFPPYLGVLAGTEYKKAMLGFLLTDPVLPILGVIITAKLGGAAEDLGKRVGNNFS
KILGTVTILTIGPLFAVPRTAATTHEVFVSKVFPGTPLWVTSLIFFILTAIFVFNETGVIDKIGKYLTPGLVIILLVIIV
KCIVDPIGVMNVATENQIFLKGFAEGYQTMDALGAALMTGIVVSDLKRKGYLNEQERVKLVKWVGLVAFLLLAIIYGGLI
YVGATASKIYTVQYTRVDILLGTVQHILGSAGKFAIGIAVSLACLTTSVGLSAVAGDFLSGITKNKLGYKPIVLATVFVS
GLISLAGVEGLIKAAVPILSTVYPIIMVLIFLGIFDKYIKYDMTFKGAVLATFVVSLVEVLNSKFKILEGFTDVIKTLPL
SSVGFEWLLPAIVTGAIFGLIAYIQDKRKIA

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=424, Percent_Identity=32.311320754717, Blast_Score=208, Evalue=6e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 46147; Mature: 46147

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]