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The map label for this gene is mtbC1 [H]

Identifier: 169823929

GI number: 169823929

Start: 274577

End: 275230

Strand: Direct

Name: mtbC1 [H]

Synonym: FMG_0232

Alternate gene names: 169823929

Gene position: 274577-275230 (Clockwise)

Preceding gene: 169823928

Following gene: 169823930

Centisome position: 15.27

GC content: 35.78

Gene sequence:

>654_bases
ATGGATACACAACAAGAATATTTAGATAAAATGGCAGAAATGGTAGTAGAAATGGAAGACGAAGATATTGCAGATGTGTG
TCAAGAATACATCGACAAGGGATTTGATCCTAAAAAAGGTATCTTCGATGGACTTATTAGAGGCATGACAGAAGCGAATA
AACTTTACGACGAAGAAGAATATTTCATCACAGATGTATTGCTTTGCAGTGATGCTATGTATGCTGGCTTGAATGTTTTA
GAAAAATACGTTGAAATAAGCGACGAAGATATTCTAGGAGTTGCAGTTATCGGTGTTGTCGAAGGTGATACTCATGATAT
CGGCAAAAATTTGGTTAAATTAATGATGGAGACTGCTGGATTCAAAATGATTGATTTGGGTCGTGATGTTAAATTGCAAA
ACTTCATAGATGCAGCAGTAGAAAACAATGCTGATTTGATTTGTATGTCAACTTTGATGACAACTACTATGGAAAACATG
GGAAAAGTTATCGAAATGTTGAAGGAACAAGGACTTAGAGATAGATTCAAAGTAATGATTGGTGGAGGACCAATTTCACA
AGGTTTTGCAGACAAAATCGGCGCAGATTCATACACAGAAAATGCCGTCGAAGCTGTAAAGGCTGCTAAGAAATTAATTG
GAAAAGATAATTAG

Upstream 100 bases:

>100_bases
TTCTTGTTTGAAGGAGAACACAAGACTTTTGGAGACAGATGTATGAATTTGTCAAACAAGAAATTCAAGAAAATAAATGA
AATCATAATTAGGAGTTAAC

Downstream 100 bases:

>100_bases
ATGTGATTAACGAAAAAGATTGATTAAAACAAGATTTACATCATATTTTCGTAAATCGAATTCCAGTGATTTGTCCAGGC
GGAGATAGCCGCTTGAAAAC

Product: putative dimethylamine corrinoid protein

Products: L-Methionine; 5,6,7,8-Tetrahydrofolate [C]

Alternate protein names: NA

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MDTQQEYLDKMAEMVVEMEDEDIADVCQEYIDKGFDPKKGIFDGLIRGMTEANKLYDEEEYFITDVLLCSDAMYAGLNVL
EKYVEISDEDILGVAVIGVVEGDTHDIGKNLVKLMMETAGFKMIDLGRDVKLQNFIDAAVENNADLICMSTLMTTTMENM
GKVIEMLKEQGLRDRFKVMIGGGPISQGFADKIGADSYTENAVEAVKAAKKLIGKDN

Sequences:

>Translated_217_residues
MDTQQEYLDKMAEMVVEMEDEDIADVCQEYIDKGFDPKKGIFDGLIRGMTEANKLYDEEEYFITDVLLCSDAMYAGLNVL
EKYVEISDEDILGVAVIGVVEGDTHDIGKNLVKLMMETAGFKMIDLGRDVKLQNFIDAAVENNADLICMSTLMTTTMENM
GKVIEMLKEQGLRDRFKVMIGGGPISQGFADKIGADSYTENAVEAVKAAKKLIGKDN
>Mature_217_residues
MDTQQEYLDKMAEMVVEMEDEDIADVCQEYIDKGFDPKKGIFDGLIRGMTEANKLYDEEEYFITDVLLCSDAMYAGLNVL
EKYVEISDEDILGVAVIGVVEGDTHDIGKNLVKLMMETAGFKMIDLGRDVKLQNFIDAAVENNADLICMSTLMTTTMENM
GKVIEMLKEQGLRDRFKVMIGGGPISQGFADKIGADSYTENAVEAVKAAKKLIGKDN

Specific function: Acts as a methyl group carrier between mtbB and mtbA [H]

COG id: COG5012

COG function: function code R; Predicted cobalamin binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=183, Percent_Identity=27.3224043715847, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1790450, Length=178, Percent_Identity=32.0224719101124, Blast_Score=82, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17535405, Length=182, Percent_Identity=28.021978021978, Blast_Score=77, Evalue=9e-15,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR003759
- InterPro:   IPR012741 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2 [H]

EC number: 2.1.1.13 [C]

Molecular weight: Translated: 24086; Mature: 24086

Theoretical pI: Translated: 4.05; Mature: 4.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
6.9 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
6.9 %Met     (Mature Protein)
8.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTQQEYLDKMAEMVVEMEDEDIADVCQEYIDKGFDPKKGIFDGLIRGMTEANKLYDEEE
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHH
YFITDVLLCSDAMYAGLNVLEKYVEISDEDILGVAVIGVVEGDTHDIGKNLVKLMMETAG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEEEEECCCHHHHHHHHHHHHHHHCC
FKMIDLGRDVKLQNFIDAAVENNADLICMSTLMTTTMENMGKVIEMLKEQGLRDRFKVMI
CEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEE
GGGPISQGFADKIGADSYTENAVEAVKAAKKLIGKDN
CCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDTQQEYLDKMAEMVVEMEDEDIADVCQEYIDKGFDPKKGIFDGLIRGMTEANKLYDEEE
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHH
YFITDVLLCSDAMYAGLNVLEKYVEISDEDILGVAVIGVVEGDTHDIGKNLVKLMMETAG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEEEEECCCHHHHHHHHHHHHHHHCC
FKMIDLGRDVKLQNFIDAAVENNADLICMSTLMTTTMENMGKVIEMLKEQGLRDRFKVMI
CEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEE
GGGPISQGFADKIGADSYTENAVEAVKAAKKLIGKDN
CCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; Cobinamide; S-adenosyl-L-methionine [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 500-780 [C]

Specific activity: 3.8

Km value (mM): 0.06 {methyltetrahydrofolate}} 0.03 {methyltetrahydrofolate}} 0.016 {homocysteine}} 0.035 {S-methyltetrahydrofolate}} 0.0016 {S-adenosyl-L-methionine}} 0.00065 {S-adenosyl-L-methionine}} [C]

Substrates: 5-Methyltetrahydrofolate; L-Homocysteine [C]

Specific reaction: 5-Methyltetrahydrofolate + L-Homocysteine --> L-Methionine + 5,6,7,8-Tetrahydrofolate [C]

General reaction: Methyl group transfer [C]

Inhibitor: 5, 6-Dimethyl benzimidazole; alpha-Ribazole 3'-phosphate; Be2+; Cobinamide; Propyl iodide; Sr2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824 [H]