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The map label for this gene is sigB [H]

Identifier: 169823706

GI number: 169823706

Start: 9740

End: 10516

Strand: Direct

Name: sigB [H]

Synonym: FMG_0009

Alternate gene names: 169823706

Gene position: 9740-10516 (Clockwise)

Preceding gene: 169823705

Following gene: 169823707

Centisome position: 0.54

GC content: 37.97

Gene sequence:

>777_bases
ATGGCGAATAAATCAATTAGTAAAGAAGAACAGGAAAATATTAAAGAATTATTCTTGAAATTCCAAGAAACACGAGACAA
GAAAACAAGGGATACTCTCGTGGAAAAAAACCTGTATATCGCTGAAATATTAGCAAAAAAATACACAGGAAGGGGAATCG
AATACGATGATTTGTACCAAGTCGCATGTATCGGACTGATTTATGCGATTGACCGCTACGATCCATCAAAAGGCTACGAG
TTCTCCTCTTATGCAACTCCTACAATCGTCGGAGAAATCAAAAAATATTTCAGAGACAAAGGCTGGGTCATCAGAGTTCC
ACGCAGAATTCAAGAGCTCAACAAAAAAGTTGCGACAGCCAAGGTTCATTTGTCACAAACAATGCAAAAATCTCCAACAG
TTGAAGACATCGCAGATTATCTGTCGATATCAAACGAAGAAGTGTTGGAAGTGTTGGAAGGAGCGAAGGTCTACGCCCCA
CAATCATTGGATCAATCGATCGATCAATCAAATGAAGACAGGGAAACTAATCTTAGCGATTTGATTGGAGAAGAAGACAA
GGACATGCTGAAGTTCGAAAACAGAGATTTAATCAACAGATGCATGCAAGATTTGAACAAAGTTGAAAGGAAAATTCTTC
TGGGAAGATATTTTGACAAGAAAACACAAATCGTAATCGCAGAGGAATTGGATATTTCGCAAATGACTGTCAGCAGAATG
GAGAAGAAAATTCTGAAAAAATTCAAGGCTGCACTCAAGGAGCAACTAGAATTTTAA

Upstream 100 bases:

>100_bases
GAAAAGGATAAAATATCAGAAATGATTATCCAATCATTAATGGATGAATACGAAATCAATGGAAGTGAGATAAAAGTTAT
CAAGAATAGGAAATAATATT

Downstream 100 bases:

>100_bases
ATGGAAATATTGAGATTTGAAAATGTTAAATTCATCAAGGAATACGATGATTTTACTATAAATAAAAACGAAATAGTTTT
TATTGTGGGAAAGAGTGGAA

Product: RNA polymerase sigma B factor

Products: NA

Alternate protein names: General stress protein 84; GSP84; Sigma-37 [H]

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MANKSISKEEQENIKELFLKFQETRDKKTRDTLVEKNLYIAEILAKKYTGRGIEYDDLYQVACIGLIYAIDRYDPSKGYE
FSSYATPTIVGEIKKYFRDKGWVIRVPRRIQELNKKVATAKVHLSQTMQKSPTVEDIADYLSISNEEVLEVLEGAKVYAP
QSLDQSIDQSNEDRETNLSDLIGEEDKDMLKFENRDLINRCMQDLNKVERKILLGRYFDKKTQIVIAEELDISQMTVSRM
EKKILKKFKAALKEQLEF

Sequences:

>Translated_258_residues
MANKSISKEEQENIKELFLKFQETRDKKTRDTLVEKNLYIAEILAKKYTGRGIEYDDLYQVACIGLIYAIDRYDPSKGYE
FSSYATPTIVGEIKKYFRDKGWVIRVPRRIQELNKKVATAKVHLSQTMQKSPTVEDIADYLSISNEEVLEVLEGAKVYAP
QSLDQSIDQSNEDRETNLSDLIGEEDKDMLKFENRDLINRCMQDLNKVERKILLGRYFDKKTQIVIAEELDISQMTVSRM
EKKILKKFKAALKEQLEF
>Mature_257_residues
ANKSISKEEQENIKELFLKFQETRDKKTRDTLVEKNLYIAEILAKKYTGRGIEYDDLYQVACIGLIYAIDRYDPSKGYEF
SSYATPTIVGEIKKYFRDKGWVIRVPRRIQELNKKVATAKVHLSQTMQKSPTVEDIADYLSISNEEVLEVLEGAKVYAPQ
SLDQSIDQSNEDRETNLSDLIGEEDKDMLKFENRDLINRCMQDLNKVERKILLGRYFDKKTQIVIAEELDISQMTVSRME
KKILKKFKAALKEQLEF

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma B is not essential for sporulation; rather it is required for maximal expression of ctc and csbA which are transcr

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. SigB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=242, Percent_Identity=28.5123966942149, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789098, Length=240, Percent_Identity=26.25, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788231, Length=203, Percent_Identity=25.1231527093596, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789871, Length=273, Percent_Identity=27.1062271062271, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014288
- InterPro:   IPR014322
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 30089; Mature: 29958

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANKSISKEEQENIKELFLKFQETRDKKTRDTLVEKNLYIAEILAKKYTGRGIEYDDLYQ
CCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHH
VACIGLIYAIDRYDPSKGYEFSSYATPTIVGEIKKYFRDKGWVIRVPRRIQELNKKVATA
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHH
KVHLSQTMQKSPTVEDIADYLSISNEEVLEVLEGAKVYAPQSLDQSIDQSNEDRETNLSD
HHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCHHCCHHH
LIGEEDKDMLKFENRDLINRCMQDLNKVERKILLGRYFDKKTQIVIAEELDISQMTVSRM
HHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
EKKILKKFKAALKEQLEF
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ANKSISKEEQENIKELFLKFQETRDKKTRDTLVEKNLYIAEILAKKYTGRGIEYDDLYQ
CCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHH
VACIGLIYAIDRYDPSKGYEFSSYATPTIVGEIKKYFRDKGWVIRVPRRIQELNKKVATA
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHH
KVHLSQTMQKSPTVEDIADYLSISNEEVLEVLEGAKVYAPQSLDQSIDQSNEDRETNLSD
HHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCHHCCHHH
LIGEEDKDMLKFENRDLINRCMQDLNKVERKILLGRYFDKKTQIVIAEELDISQMTVSRM
HHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
EKKILKKFKAALKEQLEF
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3016731; 3027048; 2170324; 9384377; 9298659 [H]