Click here to switch to the map view.

The map label for this gene is truA [H]

Identifier: 169630847

GI number: 169630847

Start: 3825569

End: 3826453

Strand: Reverse

Name: truA [H]

Synonym: MAB_3768c

Alternate gene names: 169630847

Gene position: 3826453-3825569 (Counterclockwise)

Preceding gene: 169630848

Following gene: 169630846

Centisome position: 75.51

GC content: 65.99

Gene sequence:

>885_bases
TTGGTCACATCTCACGACCAGCCCGCCATCCCTGATGGCGGGCTGGTTCGTTTGCGGCTCGATATCGCCTATGACGGAAC
CGATTTCGCGGGATGGGCCACTCAGGTAAACCAGCGCACGGTCGCCGGGGTGCTGGGCGAGGCATTCACGACCATCGTGG
GTGATTCGGTCCAGCTCTATGCCGCGGGACGTACCGACGCGGGTGTGCACGCGACGGGCCAGGTTGCCCACCTGGATGTT
CCGGTCGCCTGCCTACCCGGGTTGCGCGGCCGCTACCGCCCCGCGGGCGCACCGGAATTCGACAGGCTGGTTCGGCGGCT
GTCACGCTTCCTGCCCAGCGATGTACGCGTGCTCGATATCTCGCGCGCTCCGGCACATTTCGATGCGCGTTTCTCGGCGC
TGCGGCGGCATTATGAGTACCGGTTGTCCACGGCATCTTTTGGGGTGCTGCCACTGGCGCGTCACGATGTCGTGGGTTGG
CATCGTCCGCTGGATGTCGATGCGATGAACGAGGCCTCACGGAACTTGTTGGGGTTGCGCGATTTCGCGGCTTTCTGCCG
CCACCGCGAGGGCGCCACGACCATCCGTGAGCTGCAGCGCTTCGACTGGGGCGTGGATGGGCAGCGACTGACCGCGTACG
TGAGCGCCGACGCCTTCTGCTGGTCCATGGTGCGCTCGCTGGTGGGCGCGGTACTGGCTGTGGGGGAGGGTAGGCGGCCG
GTGCAGTGGTGCGTCGACCTGCTGAATAAAGAAAGGCGCTCAAGTGATTTCGCGGCGGCCCCGGCGCGTGGGCTGACCCT
GGTCGGGGTTGATTATCCGCCGGACGACCAGATGGCGGCCCGGGTGATGATCACCCGTGACGTGCGTATCAGACCGTCCG
GCTAG

Upstream 100 bases:

>100_bases
CGAGGTCGAGGCCGATACCGCCGACGACGCGGCCGCCGAGGTCGAAGGCGCGGCAGACACCGCCGATGCGGCTGAGGACG
CAGACAAGAAGGACTGACCT

Downstream 100 bases:

>100_bases
CCTTTCGGCATGAGGAACGTTCGTTGGATCGGGGCGGTCGCTGTCGCGGGCGCGATGTGCGTTGCCGGGTGCGGGGGCGG
ATCGACAACGGAGATTCCGC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MVTSHDQPAIPDGGLVRLRLDIAYDGTDFAGWATQVNQRTVAGVLGEAFTTIVGDSVQLYAAGRTDAGVHATGQVAHLDV
PVACLPGLRGRYRPAGAPEFDRLVRRLSRFLPSDVRVLDISRAPAHFDARFSALRRHYEYRLSTASFGVLPLARHDVVGW
HRPLDVDAMNEASRNLLGLRDFAAFCRHREGATTIRELQRFDWGVDGQRLTAYVSADAFCWSMVRSLVGAVLAVGEGRRP
VQWCVDLLNKERRSSDFAAAPARGLTLVGVDYPPDDQMAARVMITRDVRIRPSG

Sequences:

>Translated_294_residues
MVTSHDQPAIPDGGLVRLRLDIAYDGTDFAGWATQVNQRTVAGVLGEAFTTIVGDSVQLYAAGRTDAGVHATGQVAHLDV
PVACLPGLRGRYRPAGAPEFDRLVRRLSRFLPSDVRVLDISRAPAHFDARFSALRRHYEYRLSTASFGVLPLARHDVVGW
HRPLDVDAMNEASRNLLGLRDFAAFCRHREGATTIRELQRFDWGVDGQRLTAYVSADAFCWSMVRSLVGAVLAVGEGRRP
VQWCVDLLNKERRSSDFAAAPARGLTLVGVDYPPDDQMAARVMITRDVRIRPSG
>Mature_294_residues
MVTSHDQPAIPDGGLVRLRLDIAYDGTDFAGWATQVNQRTVAGVLGEAFTTIVGDSVQLYAAGRTDAGVHATGQVAHLDV
PVACLPGLRGRYRPAGAPEFDRLVRRLSRFLPSDVRVLDISRAPAHFDARFSALRRHYEYRLSTASFGVLPLARHDVVGW
HRPLDVDAMNEASRNLLGLRDFAAFCRHREGATTIRELQRFDWGVDGQRLTAYVSADAFCWSMVRSLVGAVLAVGEGRRP
VQWCVDLLNKERRSSDFAAAPARGLTLVGVDYPPDDQMAARVMITRDVRIRPSG

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=296, Percent_Identity=31.7567567567568, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI199559785, Length=288, Percent_Identity=27.0833333333333, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788657, Length=263, Percent_Identity=32.6996197718631, Blast_Score=130, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6321112, Length=269, Percent_Identity=26.7657992565056, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 32392; Mature: 32392

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTSHDQPAIPDGGLVRLRLDIAYDGTDFAGWATQVNQRTVAGVLGEAFTTIVGDSVQLY
CCCCCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
AAGRTDAGVHATGQVAHLDVPVACLPGLRGRYRPAGAPEFDRLVRRLSRFLPSDVRVLDI
ECCCCCCCCCCCCCEEEECCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEE
SRAPAHFDARFSALRRHYEYRLSTASFGVLPLARHDVVGWHRPLDVDAMNEASRNLLGLR
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHH
DFAAFCRHREGATTIRELQRFDWGVDGQRLTAYVSADAFCWSMVRSLVGAVLAVGEGRRP
HHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCH
VQWCVDLLNKERRSSDFAAAPARGLTLVGVDYPPDDQMAARVMITRDVRIRPSG
HHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHEEEEEEECEEEECCC
>Mature Secondary Structure
MVTSHDQPAIPDGGLVRLRLDIAYDGTDFAGWATQVNQRTVAGVLGEAFTTIVGDSVQLY
CCCCCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
AAGRTDAGVHATGQVAHLDVPVACLPGLRGRYRPAGAPEFDRLVRRLSRFLPSDVRVLDI
ECCCCCCCCCCCCCEEEECCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEE
SRAPAHFDARFSALRRHYEYRLSTASFGVLPLARHDVVGWHRPLDVDAMNEASRNLLGLR
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHH
DFAAFCRHREGATTIRELQRFDWGVDGQRLTAYVSADAFCWSMVRSLVGAVLAVGEGRRP
HHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCH
VQWCVDLLNKERRSSDFAAAPARGLTLVGVDYPPDDQMAARVMITRDVRIRPSG
HHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHEEEEEEECEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]